(Circular) (Six-base) MAPSORT of: pFN21AE9671.seq Check: 5621 from: 1 to: 9553 pFN21AE9671 9553 bp With 182 enzymes: SgfI * May 24, 2012 15:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3673 6718 3673 Size: 3045 6508 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2323 7363 278 Size: 53 83 186 936 787 5040 2468 Fragments arranged by size: 5040 2468 936 787 186 83 53 AccI GT'mk_AC Cuts at: 1937 3876 6374 6896 1937 Size: 1939 2498 522 4594 Fragments arranged by size: 4594 2498 1939 522 Acc65I G'GTAC_C Cuts at: 6874 6874 Size: 9553 AclI AA'CG_TT Cuts at: 1563 7680 8053 1563 Size: 6117 373 3063 Fragments arranged by size: 6117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 3217 3424 5609 5674 5864 6556 7616 Size: 1166 419 207 2185 65 190 692 1060 Cuts at: 7616 9000 9268 1632 Size: 1384 268 1917 Fragments arranged by size: 2185 1917 1384 1166 1060 692 419 268 207 190 65 AfeI AGC'GCT Cuts at: 5831 5831 Size: 9553 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9356 AflIII A'CryG_T Cuts at: 1203 6000 8452 9429 1203 Size: 4797 2452 977 1327 Fragments arranged by size: 4797 2452 1327 977 AgeI A'CCGG_T Cuts at: 8384 8384 Size: 9553 AhdI GACnn_n'nnGTC Cuts at: 6115 8282 6115 Size: 2167 7386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5600 6021 6758 1184 Size: 4416 421 737 3979 Fragments arranged by size: 4416 3979 737 421 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4655 4687 5804 5836 4655 Size: 32 1117 32 8372 Fragments arranged by size: 8372 1117 32 32 AlwNI CAG_nnn'CTG Cuts at: 2776 3082 3145 5635 5766 7296 8868 2776 Size: 306 63 2490 131 1530 1572 3461 Fragments arranged by size: 3461 2490 1572 1530 306 131 63 ApaI G_GGCC'C Cuts at: 2528 6418 2528 Size: 3890 5663 ApaLI G'TGCA_C Cuts at: 5884 7610 8766 5884 Size: 1726 1156 6671 Fragments arranged by size: 6671 1726 1156 ApoI r'AATT_y Cuts at: 1493 2156 4028 4676 6862 7088 7240 9349 1493 Size: 663 1872 648 2186 226 152 2109 1697 Fragments arranged by size: 2186 2109 1872 1697 663 648 226 152 AseI AT'TA_AT Cuts at: 160 1032 8107 160 Size: 872 7075 1606 Fragments arranged by size: 7075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9553 AvaI C'yCGr_G Cuts at: 1870 1943 2028 3482 4418 4556 4745 5210 5261 Size: 73 85 1454 936 138 189 465 51 Cuts at: 5261 6012 6079 6554 6593 6804 6878 1870 Size: 751 67 475 39 211 74 4545 Fragments arranged by size: 4545 1454 936 751 475 465 211 189 138 85 74 73 67 51 39 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4310 4343 4310 Size: 33 9520 BamHI G'GATC_C Cuts at: 3472 4552 6883 7250 3472 Size: 1080 2331 367 5775 Fragments arranged by size: 5775 2331 1080 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 6350 6498 6874 8329 Size: 325 217 627 245 4318 148 376 1455 Cuts at: 8329 618 Size: 1842 Fragments arranged by size: 4318 1842 1455 627 376 325 245 217 148 BanII G_rGCy'C Cuts at: 1391 2528 3796 4893 4974 6080 6418 6600 6872 Size: 1137 1268 1097 81 1106 338 182 272 Cuts at: 6872 1391 Size: 4072 Fragments arranged by size: 4072 1268 1137 1106 1097 338 272 182 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2598 3936 4592 928 Size: 971 699 1338 656 5889 Fragments arranged by size: 5889 1338 971 699 656 BbvCI CC'TCA_GC Cuts at: 5759 6817 5759 Size: 1058 8495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 7744 7778 1903 Size: 34 50 34 5723 34 3678 Fragments arranged by size: 5723 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7445 8655 7445 Size: 1210 8343 BclI T'GATC_A Cuts at: 1548 3585 3756 4612 1548 Size: 2037 171 856 6489 Fragments arranged by size: 6489 2037 856 171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2051 4700 Size: 107 122 71 631 605 337 42 2649 Cuts at: 4700 8164 136 Size: 3464 1525 Fragments arranged by size: 3464 2649 1525 631 605 337 122 107 71 42 BglII A'GATC_T Cuts at: 2878 3519 3882 4998 6159 9548 2878 Size: 641 363 1116 1161 3389 2883 Fragments arranged by size: 3389 2883 1161 1116 641 363 BlpI GC'TnA_GC Cuts at: 2445 6026 6970 2445 Size: 3581 944 5028 Fragments arranged by size: 5028 3581 944 Bme1580I G_kGCm'C Cuts at: 1790 2037 2528 4696 5888 6418 6667 7614 8770 Size: 247 491 2168 1192 530 249 947 1156 Cuts at: 8770 1790 Size: 2573 Fragments arranged by size: 2573 2168 1192 1156 947 530 491 249 247 BmgBI CAC'GTC Cuts at: 1318 4428 5653 1318 Size: 3110 1225 5218 Fragments arranged by size: 5218 3110 1225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3471 4540 6541 Size: 416 53 83 399 24 2054 1069 2001 Cuts at: 6541 7284 8242 442 Size: 743 958 1753 Fragments arranged by size: 2054 2001 1753 1069 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9553 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5990 6832 5990 Size: 842 8711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 4589 4757 5082 5155 5819 5985 6176 Size: 3221 129 168 325 73 664 166 191 Cuts at: 6176 8213 1239 Size: 2037 2579 Fragments arranged by size: 3221 2579 2037 664 325 191 168 166 129 73 Bpu10I CC'TnA_GC Cuts at: 4968 5759 6817 6846 4968 Size: 791 1058 29 7675 Fragments arranged by size: 7675 1058 791 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7678 8543 8841 7678 Size: 865 298 8390 Fragments arranged by size: 8390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3593 5603 8216 882 Size: 617 2094 2010 2613 2219 Fragments arranged by size: 2613 2219 2094 2010 617 BsaAI yAC'GTr Cuts at: 493 1204 2210 6001 7344 493 Size: 711 1006 3791 1343 2702 Fragments arranged by size: 3791 2702 1343 1006 711 BsaBI GATnn'nnATC Cuts at: 3524 5402 9547 3524 Size: 1878 4145 3530 Fragments arranged by size: 4145 3530 1878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2320 3737 3966 7360 Size: 53 83 186 936 787 1417 229 3394 Cuts at: 7360 7742 8377 275 Size: 382 635 1451 Fragments arranged by size: 3394 1451 1417 936 787 635 382 229 186 83 53 BsaWI w'CCGG_w Cuts at: 4923 6090 7986 8384 8658 8805 9216 4923 Size: 1167 1896 398 274 147 411 5260 Fragments arranged by size: 5260 1896 1167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3037 3067 3595 3625 4200 Size: 30 165 30 1634 30 528 30 575 Cuts at: 4200 4230 5537 5567 5648 5678 5804 5834 6167 Size: 30 1307 30 81 30 126 30 333 Cuts at: 6167 6197 1178 Size: 30 4534 Fragments arranged by size: 4534 1634 1307 575 528 333 165 126 81 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2437 2798 2981 5159 6848 1186 Size: 1057 194 361 183 2178 1689 3891 Fragments arranged by size: 3891 2178 1689 1057 361 194 183 BseYI C'CCAG_C Cuts at: 2023 3213 3767 3778 4400 4650 5603 8756 9252 Size: 1190 554 11 622 250 953 3153 496 Cuts at: 9252 2023 Size: 2324 Fragments arranged by size: 3153 2324 1190 953 622 554 496 250 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2834 5167 5192 5981 1163 Size: 573 1098 2333 25 789 4735 Fragments arranged by size: 4735 2333 1098 789 573 25 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6400 7003 7764 8792 1540 Size: 275 186 4399 603 761 1028 2301 Fragments arranged by size: 4399 2301 1028 761 603 275 186 BsiHKAI G_wGCw'C Cuts at: 2293 4920 5888 6080 6624 7614 7699 8770 2293 Size: 2627 968 192 544 990 85 1071 3076 Fragments arranged by size: 3076 2627 1071 990 968 544 192 85 BsmI GAATG_Cn' Cuts at: 3063 3820 7064 7157 3063 Size: 757 3244 93 5459 Fragments arranged by size: 5459 3244 757 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2608 2832 2968 5643 2608 Size: 224 136 2675 6518 Fragments arranged by size: 6518 2675 224 136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2293 2528 3796 4696 4893 4920 Size: 399 247 256 235 1268 900 197 27 Cuts at: 4920 4974 5888 6080 6418 6600 6624 6667 6872 Size: 54 914 192 338 182 24 43 205 Cuts at: 6872 7614 7699 8770 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1268 1071 914 900 742 399 338 256 247 235 205 197 192 182 85 54 43 27 24 BspEI T'CCGG_A Cuts at: 6090 9216 6090 Size: 3126 6427 BspHI T'CATG_A Cuts at: 3033 7442 9172 3033 Size: 4409 1730 3414 Fragments arranged by size: 4409 3414 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3236 3673 4833 5133 5214 6132 6718 6908 Size: 2392 437 1160 300 81 918 586 190 Cuts at: 6908 844 Size: 3489 Fragments arranged by size: 3489 2392 1160 918 586 437 300 190 81 BsrBI CCG'CTC Cuts at: 1394 1823 4069 6669 7440 1394 Size: 429 2246 2600 771 3507 Fragments arranged by size: 3507 2600 2246 771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6742 8048 8222 65 Size: 1183 5494 1306 174 1396 Fragments arranged by size: 5494 1396 1306 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3275 3954 5778 5906 6446 6519 Size: 110 198 1267 679 1824 128 540 73 Cuts at: 6519 6693 6723 8197 8384 1700 Size: 174 30 1474 187 2869 Fragments arranged by size: 2869 1824 1474 1267 679 540 198 187 174 128 110 73 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9553 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9553 BssSI C'ACGA_G Cuts at: 7613 8625 7613 Size: 1012 8541 BstAPI GCAn_nnn'nTGC Cuts at: 794 3069 6456 794 Size: 2275 3387 3891 Fragments arranged by size: 3891 3387 2275 BstBI TT'CG_AA Cuts at: 8357 8357 Size: 9553 BstEII G'GTnAC_C Cuts at: 1184 3254 1184 Size: 2070 7483 BstXI CCAn_nnnn'nTGG Cuts at: 3871 4735 5950 6536 6772 3871 Size: 864 1215 586 236 6652 Fragments arranged by size: 6652 1215 864 586 236 BstYI r'GATC_y Cuts at: 1972 2878 3472 3519 3882 4552 4998 5296 6159 Size: 906 594 47 363 670 446 298 863 Cuts at: 6159 6883 7250 7635 7652 9104 9190 9202 9548 Size: 724 367 385 17 1452 86 12 346 Cuts at: 9548 1972 Size: 1977 Fragments arranged by size: 1977 1452 906 863 724 670 594 446 385 367 363 346 298 86 47 17 12 BstZ17I GTA'TAC Cuts at: 6375 6375 Size: 9553 Bsu36I CC'TnA_GG Cuts at: 2544 4833 6230 2544 Size: 2289 1397 5867 Fragments arranged by size: 5867 2289 1397 BtgI C'CryG_G Cuts at: 513 1065 3603 4536 4880 4893 5151 5187 5232 Size: 552 2538 933 344 13 258 36 45 Cuts at: 5232 5623 5835 5955 6354 6421 6600 513 Size: 391 212 120 399 67 179 3466 Fragments arranged by size: 3466 2538 933 552 399 391 344 258 212 179 120 67 45 36 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3973 487 Size: 683 56 2747 6067 Fragments arranged by size: 6067 2747 683 56 BtsI GCAGTG_nn' Cuts at: 4045 5993 7069 7863 7883 4045 Size: 1948 1076 794 20 5715 Fragments arranged by size: 5715 1948 1076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9518 DraI TTT'AAA Cuts at: 2262 6857 2262 Size: 4595 4958 DraIII CAC_nnn'GTG Cuts at: 5496 6643 5496 Size: 1147 8406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2742 8560 809 Size: 507 1426 5818 1802 Fragments arranged by size: 5818 1802 1426 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2052 4086 Size: 54 1425 50 275 18 213 9 2034 Cuts at: 4086 4984 5626 5952 6357 6397 6756 6795 7000 Size: 898 642 326 405 40 359 39 205 Cuts at: 7000 7889 9518 8 Size: 889 1629 43 Fragments arranged by size: 2034 1629 1425 898 889 642 405 359 326 275 213 205 54 50 43 40 39 18 9 EagI C'GGCC_G Cuts at: 1537 1812 6397 7000 1537 Size: 275 4585 603 4090 Fragments arranged by size: 4585 4090 603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2544 2606 2966 3308 4229 5009 6704 7483 Size: 1191 62 360 342 921 780 1695 779 Cuts at: 7483 1353 Size: 3423 Fragments arranged by size: 3423 1695 1191 921 780 779 360 342 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8140 8514 8660 387 Size: 1064 138 6551 374 146 1280 Fragments arranged by size: 6551 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6078 6078 Size: 9553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2798 3217 3424 4460 4589 4757 5082 Size: 393 1166 419 207 1036 129 168 325 Cuts at: 5082 5155 5609 5674 5819 5864 5985 6176 6556 Size: 73 454 65 145 45 121 191 380 Cuts at: 6556 7616 8213 9000 9268 1239 Size: 1060 597 787 268 1524 Fragments arranged by size: 1524 1166 1060 1036 787 597 454 419 393 380 325 268 207 191 168 145 129 121 73 65 45 EcoNI CCTnn'n_nnAGG Cuts at: 4568 6128 4568 Size: 1560 7993 EcoO109I rG'GnC_Cy Cuts at: 2524 2525 3427 3580 3751 3845 4732 4750 4765 Size: 1 902 153 171 94 887 18 15 Cuts at: 4765 4903 5203 5515 6083 6414 6415 2524 Size: 138 300 312 568 331 1 5662 Fragments arranged by size: 5662 902 887 568 331 312 300 171 153 138 94 18 15 1 1 EcoRI G'AATT_C Cuts at: 6862 7240 6862 Size: 378 9175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4609 4609 Size: 9553 FspI TGC'GCA Cuts at: 8059 8059 Size: 9553 HaeII r_GCGC'y Cuts at: 1678 3209 3955 4349 5833 8700 1678 Size: 1531 746 394 1484 2867 2531 Fragments arranged by size: 2867 2531 1531 1484 746 394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3037 3069 3146 3178 3329 Size: 32 135 32 1465 32 77 32 151 Cuts at: 3329 3361 3951 3983 4127 4159 4200 4232 4277 Size: 32 590 32 144 32 41 32 45 Cuts at: 4277 4298 4309 4330 4544 4576 4990 5022 6067 Size: 21 11 21 214 32 414 32 1045 Cuts at: 6067 6099 6101 6133 6167 6199 8194 8226 8268 Size: 32 2 32 34 32 1995 32 42 Cuts at: 8268 8300 1373 Size: 32 2626 Fragments arranged by size: 2626 1995 1465 1045 590 414 214 151 144 135 77 45 42 41 34 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 21 11 2 HincII GTy'rAC Cuts at: 669 1657 1938 2870 5059 6897 7143 669 Size: 988 281 932 2189 1838 246 3079 Fragments arranged by size: 3079 2189 1838 988 932 281 246 HindIII A'AGCT_T Cuts at: 748 5287 6782 748 Size: 4539 1495 3519 Fragments arranged by size: 4539 3519 1495 HpaI GTT'AAC Cuts at: 7143 7143 Size: 9553 KpnI G_GTAC'C Cuts at: 6878 6878 Size: 9553 MfeI C'AATT_G Cuts at: 7152 7152 Size: 9553 MluI A'CGCG_T Cuts at: 9429 9429 Size: 9553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3588 4186 4410 4959 5439 6144 8667 8851 Size: 2283 598 224 549 480 705 2523 184 Cuts at: 8851 1305 Size: 2007 Fragments arranged by size: 2523 2283 2007 705 598 549 480 224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4088 6359 6758 6797 10 Size: 54 1425 343 2256 2271 399 39 2766 Fragments arranged by size: 2766 2271 2256 1425 399 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 3467 5507 6101 6332 7511 7870 8029 Size: 648 2301 2040 594 231 1179 359 159 Cuts at: 8029 518 Size: 2042 Fragments arranged by size: 2301 2042 2040 1179 648 594 359 231 159 MspA1I CmG'CkG Cuts at: 1606 4324 4603 5181 5625 5632 5694 5859 5957 Size: 2718 279 578 444 7 62 165 98 Cuts at: 5957 6025 6969 7646 8794 9039 1606 Size: 68 944 677 1148 245 2120 Fragments arranged by size: 2718 2120 1148 944 677 578 444 279 245 165 98 68 62 7 NaeI GCC'GGC Cuts at: 2010 3956 6448 2010 Size: 1946 2492 5115 Fragments arranged by size: 5115 2492 1946 NcoI C'CATG_G Cuts at: 513 1065 4536 5232 5835 6354 6600 513 Size: 552 3471 696 603 519 246 3466 Fragments arranged by size: 3471 3466 696 603 552 519 246 NdeI CA'TA_TG Cuts at: 387 387 Size: 9553 NgoMIV G'CCGG_C Cuts at: 2008 3954 6446 2008 Size: 1946 2492 5115 Fragments arranged by size: 5115 2492 1946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9553 NotI GC'GGCC_GC Cuts at: 7000 7000 Size: 9553 NspI r_CATG'y Cuts at: 1132 1229 3748 4474 6280 6911 8456 1132 Size: 97 2519 726 1806 631 1545 2229 Fragments arranged by size: 2519 2229 1806 1545 726 631 97 PasI CC'CwG_GG Cuts at: 1348 4728 6299 6673 1348 Size: 3380 1571 374 4228 Fragments arranged by size: 4228 3380 1571 374 PciI A'CATG_T Cuts at: 8452 8452 Size: 9553 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9553 PfoI T'CCnGG_A Cuts at: 4734 5796 5985 6711 4734 Size: 1062 189 726 7576 Fragments arranged by size: 7576 1062 726 189 PmeI GTTT'AAAC Cuts at: 6857 6857 Size: 9553 PmlI CAC'GTG Cuts at: 6001 6001 Size: 9553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5804 5836 7622 7654 9135 9167 5804 Size: 32 1786 32 1481 32 6190 Fragments arranged by size: 6190 1786 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3580 3751 3845 4732 4750 4765 4903 5203 5515 Size: 171 94 887 18 15 138 300 312 Cuts at: 5515 6083 3580 Size: 568 7050 Fragments arranged by size: 7050 887 568 312 300 171 138 94 18 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9553 PsiI TTA'TAA Cuts at: 7123 7123 Size: 9553 PspOMI G'GGCC_C Cuts at: 2524 6414 2524 Size: 3890 5663 PspXI vC'TCGA_Gb Cuts at: 4745 5261 6554 4745 Size: 516 1293 7744 Fragments arranged by size: 7744 1293 516 PstI C_TGCA'G Cuts at: 830 2662 3555 5904 6905 830 Size: 1832 893 2349 1001 3478 Fragments arranged by size: 3478 2349 1832 1001 893 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9553 PvuII CAG'CTG Cuts at: 4603 6025 4603 Size: 1422 8131 SacI G_AGCT'C Cuts at: 6080 6080 Size: 9553 SacII CC_GC'GG Cuts at: 5626 5958 5626 Size: 332 9221 SalI G'TCGA_C Cuts at: 1936 6895 1936 Size: 4959 4594 SanDI GG'GwC_CC Cuts at: 3845 5515 3845 Size: 1670 7883 SapI GCTCTTCn'nnn_ Cuts at: 5009 6704 5009 Size: 1695 7858 SbfI CC_TGCA'GG Cuts at: 6905 6905 Size: 9553 ScaI AGT'ACT Cuts at: 7801 9513 7801 Size: 1712 7841 SexAI A'CCwGG_T Cuts at: 6068 6068 Size: 9553 SfcI C'TryA_G Cuts at: 826 1045 2658 3551 5068 5900 6901 8036 8717 Size: 219 1613 893 1517 832 1001 1135 681 Cuts at: 8717 8908 826 Size: 191 1471 Fragments arranged by size: 1613 1517 1471 1135 1001 893 832 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2051 2051 Size: 9553 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9553 SgrAI Cr'CCGG_yG Cuts at: 1700 3954 1700 Size: 2254 7299 SmaI CCC'GGG Cuts at: 1872 2030 6014 6880 1872 Size: 158 3984 866 4545 Fragments arranged by size: 4545 3984 866 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 4745 5261 6554 7657 8558 8820 Size: 197 926 2802 516 1293 1103 901 262 Cuts at: 8820 820 Size: 1553 Fragments arranged by size: 2802 1553 1293 1103 926 901 516 262 197 SnaBI TAC'GTA Cuts at: 493 2210 7344 493 Size: 1717 5134 2702 Fragments arranged by size: 5134 2702 1717 SpeI A'CTAG_T Cuts at: 152 152 Size: 9553 SphI G_CATG'C Cuts at: 1132 3748 6280 6911 1132 Size: 2616 2532 631 3774 Fragments arranged by size: 3774 2616 2532 631 SspI AAT'ATT Cuts at: 5 52 7477 5 Size: 47 7425 2081 Fragments arranged by size: 7425 2081 47 StuI AGG'CCT Cuts at: 1517 4838 5074 5267 1517 Size: 3321 236 193 5803 Fragments arranged by size: 5803 3321 236 193 StyI C'CwwG_G Cuts at: 513 1065 2277 4536 5232 5835 6291 6354 6600 Size: 552 1212 2259 696 603 456 63 246 Cuts at: 6600 6992 513 Size: 392 3074 Fragments arranged by size: 3074 2259 1212 696 603 552 456 392 246 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2779 3129 4162 5099 6642 7580 7597 Size: 1255 1199 350 1033 937 1543 938 17 Cuts at: 7597 7750 7935 8380 325 Size: 153 185 445 1498 Fragments arranged by size: 1543 1498 1255 1199 1033 938 937 445 350 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3240 5226 7799 Size: 274 80 33 51 1443 1263 1986 2573 Cuts at: 7799 9511 96 Size: 1712 138 Fragments arranged by size: 2573 1986 1712 1443 1263 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2031 2063 3595 3627 5648 5680 9513 9545 2031 Size: 32 1532 32 2021 32 3833 32 2039 Fragments arranged by size: 3833 2039 2021 1532 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2072 4289 4412 1316 Size: 756 2217 123 6457 Fragments arranged by size: 6457 2217 756 123 XbaI T'CTAG_A Cuts at: 2323 6889 2323 Size: 4566 4987 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3868 6306 3868 Size: 2438 7115 XhoI C'TCGA_G Cuts at: 1943 4745 5261 6554 1943 Size: 2802 516 1293 4942 Fragments arranged by size: 4942 2802 1293 516 XmaI C'CCGG_G Cuts at: 1870 2028 6012 6878 1870 Size: 158 3984 866 4545 Fragments arranged by size: 4545 3984 866 158 XmnI GAAnn'nnTTC Cuts at: 3462 3635 5705 7682 3462 Size: 173 2070 1977 5333 Fragments arranged by size: 5333 2070 1977 173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2321 7361 276 Size: 53 83 186 936 787 5040 2468 Fragments arranged by size: 5040 2468 936 787 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BsiWI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SfoI SrfI SwaI