Restriction map for
pFN21AE9671
(Linear) (Six-base) MAPSORT of: KIEE9671.seq Check: 2885 from: 1 to: 4848
KIEE9671.orf
With 182 enzymes: SgfI *
May 24, 2012 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1668 4713 4848
Size: 1668 3045 135
Fragments arranged by size:
3045 1668 135
AatII G_ACGT'C
Cuts at: 0 318 4848
Size: 318 4530
AccI GT'mk_AC
Cuts at: 0 1871 4369 4848
Size: 1871 2498 479
Fragments arranged by size:
2498 1871 479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1212 1419 3604 3669 3859 4551 4848
Size: 793 419 207 2185 65 190 692 297
Fragments arranged by size:
2185 793 692 419 297 207 190 65
AfeI AGC'GCT
Cuts at: 0 3826 4848
Size: 3826 1022
AflIII A'CryG_T
Cuts at: 0 3995 4848
Size: 3995 853
AhdI GACnn_n'nnGTC
Cuts at: 0 4110 4848
Size: 4110 738
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3595 4016 4753 4848
Size: 3595 421 737 95
Fragments arranged by size:
3595 737 421 95
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2650 2682 3799 3831 4848
Size: 2650 32 1117 32 1017
Fragments arranged by size:
2650 1117 1017 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 771 1077 1140 3630 3761 4848
Size: 771 306 63 2490 131 1087
Fragments arranged by size:
2490 1087 771 306 131 63
ApaI G_GGCC'C
Cuts at: 0 523 4413 4848
Size: 523 3890 435
Fragments arranged by size:
3890 523 435
ApaLI G'TGCA_C
Cuts at: 0 3879 4848
Size: 3879 969
ApoI r'AATT_y
Cuts at: 0 151 2023 2671 4848
Size: 151 1872 648 2177
Fragments arranged by size:
2177 1872 648 151
AvaI C'yCGr_G
Cuts at: 0 23 1477 2413 2551 2740 3205 3256 4007
Size: 23 1454 936 138 189 465 51 751
Cuts at: 4007 4074 4549 4588 4799 4848
Size: 67 475 39 211 49
Fragments arranged by size:
1454 936 751 475 465 211 189 138
67 51 49 39 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2305 2338 4848
Size: 2305 33 2510
Fragments arranged by size:
2510 2305 33
BamHI G'GATC_C
Cuts at: 0 1467 2547 4848
Size: 1467 1080 2301
Fragments arranged by size:
2301 1467 1080
BanI G'GyrC_C
Cuts at: 0 27 4345 4493 4848
Size: 27 4318 148 355
Fragments arranged by size:
4318 355 148 27
BanII G_rGCy'C
Cuts at: 0 523 1791 2888 2969 4075 4413 4595 4848
Size: 523 1268 1097 81 1106 338 182 253
Fragments arranged by size:
1268 1106 1097 523 338 253 182 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 1931 2587 4848
Size: 593 1338 656 2261
Fragments arranged by size:
2261 1338 656 593
BbvCI CC'TCA_GC
Cuts at: 0 3754 4812 4848
Size: 3754 1058 36
Fragments arranged by size:
3754 1058 36
BclI T'GATC_A
Cuts at: 0 1580 1751 2607 4848
Size: 1580 171 856 2241
Fragments arranged by size:
2241 1580 856 171
BglI GCCn_nnn'nGGC
Cuts at: 0 46 2695 4848
Size: 46 2649 2153
Fragments arranged by size:
2649 2153 46
BglII A'GATC_T
Cuts at: 0 873 1514 1877 2993 4154 4848
Size: 873 641 363 1116 1161 694
Fragments arranged by size:
1161 1116 873 694 641 363
BlpI GC'TnA_GC
Cuts at: 0 440 4021 4848
Size: 440 3581 827
Fragments arranged by size:
3581 827 440
Bme1580I G_kGCm'C
Cuts at: 0 32 523 2691 3883 4413 4662 4848
Size: 32 491 2168 1192 530 249 186
Fragments arranged by size:
2168 1192 530 491 249 186 32
BmgBI CAC'GTC
Cuts at: 0 2423 3648 4848
Size: 2423 1225 1200
Fragments arranged by size:
2423 1225 1200
BmrI ACTGGGnnnn_n'
Cuts at: 0 1466 2535 4536 4848
Size: 1466 1069 2001 312
Fragments arranged by size:
2001 1466 1069 312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3985 4827 4848
Size: 3985 842 21
Fragments arranged by size:
3985 842 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2455 2584 2752 3077 3150 3814 3980 4171
Size: 2455 129 168 325 73 664 166 191
Cuts at: 4171 4848
Size: 677
Fragments arranged by size:
2455 677 664 325 191 168 166 129
73
Bpu10I CC'TnA_GC
Cuts at: 0 2963 3754 4812 4841 4848
Size: 2963 791 1058 29 7
Fragments arranged by size:
2963 1058 791 29 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 1588 3598 4848
Size: 1588 2010 1250
Fragments arranged by size:
2010 1588 1250
BsaAI yAC'GTr
Cuts at: 0 205 3996 4848
Size: 205 3791 852
Fragments arranged by size:
3791 852 205
BsaBI GATnn'nnATC
Cuts at: 0 1519 3397 4848
Size: 1519 1878 1451
Fragments arranged by size:
1878 1519 1451
BsaHI Gr'CG_yC
Cuts at: 0 315 1732 1961 4848
Size: 315 1417 229 2887
Fragments arranged by size:
2887 1417 315 229
BsaWI w'CCGG_w
Cuts at: 0 2918 4085 4848
Size: 2918 1167 763
Fragments arranged by size:
2918 1167 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1032 1062 1590 1620 2195 2225 3532 3562
Size: 1032 30 528 30 575 30 1307 30
Cuts at: 3562 3643 3673 3799 3829 4162 4192 4848
Size: 81 30 126 30 333 30 656
Fragments arranged by size:
1307 1032 656 575 528 333 126 81
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 432 793 976 3154 4843 4848
Size: 238 194 361 183 2178 1689 5
Fragments arranged by size:
2178 1689 361 238 194 183 5
BseYI C'CCAG_C
Cuts at: 0 18 1208 1762 1773 2395 2645 3598 4848
Size: 18 1190 554 11 622 250 953 1250
Fragments arranged by size:
1250 1190 953 622 554 250 18 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 3162 3187 3976 4848
Size: 829 2333 25 789 872
Fragments arranged by size:
2333 872 829 789 25
BsiEI CG_ry'CG
Cuts at: 0 4395 4848
Size: 4395 453
BsiHKAI G_wGCw'C
Cuts at: 0 288 2915 3883 4075 4619 4848
Size: 288 2627 968 192 544 229
Fragments arranged by size:
2627 968 544 288 229 192
BsmI GAATG_Cn'
Cuts at: 0 1058 1815 4848
Size: 1058 757 3033
Fragments arranged by size:
3033 1058 757
BsmBI CGTCTCn'nnnn_
Cuts at: 0 603 827 963 3638 4848
Size: 603 224 136 2675 1210
Fragments arranged by size:
2675 1210 603 224 136
Bsp1286I G_dGCh'C
Cuts at: 0 32 288 523 1791 2691 2888 2915 2969
Size: 32 256 235 1268 900 197 27 54
Cuts at: 2969 3883 4075 4413 4595 4619 4662 4848
Size: 914 192 338 182 24 43 186
Fragments arranged by size:
1268 914 900 338 256 235 197 192
186 182 54 43 32 27 24
BspEI T'CCGG_A
Cuts at: 0 4085 4848
Size: 4085 763
BspHI T'CATG_A
Cuts at: 0 1028 4848
Size: 1028 3820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1231 1668 2828 3128 3209 4127 4713 4848
Size: 1231 437 1160 300 81 918 586 135
Fragments arranged by size:
1231 1160 918 586 437 300 135 81
BsrBI CCG'CTC
Cuts at: 0 2064 4664 4848
Size: 2064 2600 184
Fragments arranged by size:
2600 2064 184
BsrDI GCAATG_nn'
Cuts at: 0 4737 4848
Size: 4737 111
BsrFI r'CCGG_y
Cuts at: 0 3 1270 1949 3773 3901 4441 4514 4688
Size: 3 1267 679 1824 128 540 73 174
Cuts at: 4688 4718 4848
Size: 30 130
Fragments arranged by size:
1824 1267 679 540 174 130 128 73
30 3
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1064 4451 4848
Size: 1064 3387 397
Fragments arranged by size:
3387 1064 397
BstEII G'GTnAC_C
Cuts at: 0 1249 4848
Size: 1249 3599
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1866 2730 3945 4531 4767 4848
Size: 1866 864 1215 586 236 81
Fragments arranged by size:
1866 1215 864 586 236 81
BstYI r'GATC_y
Cuts at: 0 873 1467 1514 1877 2547 2993 3291 4154
Size: 873 594 47 363 670 446 298 863
Cuts at: 4154 4848
Size: 694
Fragments arranged by size:
873 863 694 670 594 446 363 298
47
BstZ17I GTA'TAC
Cuts at: 0 4370 4848
Size: 4370 478
Bsu36I CC'TnA_GG
Cuts at: 0 539 2828 4225 4848
Size: 539 2289 1397 623
Fragments arranged by size:
2289 1397 623 539
BtgI C'CryG_G
Cuts at: 0 1598 2531 2875 2888 3146 3182 3227 3618
Size: 1598 933 344 13 258 36 45 391
Cuts at: 3618 3830 3950 4349 4416 4595 4848
Size: 212 120 399 67 179 253
Fragments arranged by size:
1598 933 399 391 344 258 253 212
179 120 67 45 36 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1968 4848
Size: 1968 2880
BtsI GCAGTG_nn'
Cuts at: 0 2040 3988 4848
Size: 2040 1948 860
Fragments arranged by size:
2040 1948 860
DraI TTT'AAA
Cuts at: 0 257 4848
Size: 257 4591
DraIII CAC_nnn'GTG
Cuts at: 0 3491 4638 4848
Size: 3491 1147 210
Fragments arranged by size:
3491 1147 210
DrdI GACnn_nn'nnGTC
Cuts at: 0 737 4848
Size: 737 4111
EaeI y'GGCC_r
Cuts at: 0 38 47 2081 2979 3621 3947 4352 4392
Size: 38 9 2034 898 642 326 405 40
Cuts at: 4392 4751 4790 4848
Size: 359 39 58
Fragments arranged by size:
2034 898 642 405 359 326 58 40
39 38 9
EagI C'GGCC_G
Cuts at: 0 4392 4848
Size: 4392 456
EarI CTCTTCn'nnn_
Cuts at: 0 539 601 961 1303 2224 3004 4699 4848
Size: 539 62 360 342 921 780 1695 149
Fragments arranged by size:
1695 921 780 539 360 342 149 62
EcoICRI GAG'CTC
Cuts at: 0 4073 4848
Size: 4073 775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1212 1419 2455 2584 2752 3077 3150
Size: 793 419 207 1036 129 168 325 73
Cuts at: 3150 3604 3669 3814 3859 3980 4171 4551 4848
Size: 454 65 145 45 121 191 380 297
Fragments arranged by size:
1036 793 454 419 380 325 297 207
191 168 145 129 121 73 65 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2563 4123 4848
Size: 2563 1560 725
Fragments arranged by size:
2563 1560 725
EcoO109I rG'GnC_Cy
Cuts at: 0 519 520 1422 1575 1746 1840 2727 2745
Size: 519 1 902 153 171 94 887 18
Cuts at: 2745 2760 2898 3198 3510 4078 4409 4410 4848
Size: 15 138 300 312 568 331 1 438
Fragments arranged by size:
902 887 568 519 438 331 312 300
171 153 138 94 18 15 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2604 4848
Size: 2604 2244
HaeII r_GCGC'y
Cuts at: 0 1204 1950 2344 3828 4848
Size: 1204 746 394 1484 1020
Fragments arranged by size:
1484 1204 1020 746 394
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1032 1064 1141 1173 1324 1356 1946 1978
Size: 1032 32 77 32 151 32 590 32
Cuts at: 1978 2122 2154 2195 2227 2272 2293 2304 2325
Size: 144 32 41 32 45 21 11 21
Cuts at: 2325 2539 2571 2985 3017 4062 4094 4096 4128
Size: 214 32 414 32 1045 32 2 32
Cuts at: 4128 4162 4194 4848
Size: 34 32 654
Fragments arranged by size:
1045 1032 654 590 414 214 151 144
77 45 41 34 32 32 32 32
32 32 32 32 32 32 32 21
21 11 2
HincII GTy'rAC
Cuts at: 0 865 3054 4848
Size: 865 2189 1794
Fragments arranged by size:
2189 1794 865
HindIII A'AGCT_T
Cuts at: 0 3282 4777 4848
Size: 3282 1495 71
Fragments arranged by size:
3282 1495 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1583 2181 2405 2954 3434 4139 4848
Size: 1583 598 224 549 480 705 709
Fragments arranged by size:
1583 709 705 598 549 480 224
MscI TGG'CCA
Cuts at: 0 2083 4354 4753 4792 4848
Size: 2083 2271 399 39 56
Fragments arranged by size:
2271 2083 399 56 39
MslI CAynn'nnrTG
Cuts at: 0 1462 3502 4096 4327 4848
Size: 1462 2040 594 231 521
Fragments arranged by size:
2040 1462 594 521 231
MspA1I CmG'CkG
Cuts at: 0 2319 2598 3176 3620 3627 3689 3854 3952
Size: 2319 279 578 444 7 62 165 98
Cuts at: 3952 4020 4848
Size: 68 828
Fragments arranged by size:
2319 828 578 444 279 165 98 68
62 7
NaeI GCC'GGC
Cuts at: 0 5 1951 4443 4848
Size: 5 1946 2492 405
Fragments arranged by size:
2492 1946 405 5
NcoI C'CATG_G
Cuts at: 0 2531 3227 3830 4349 4595 4848
Size: 2531 696 603 519 246 253
Fragments arranged by size:
2531 696 603 519 253 246
NgoMIV G'CCGG_C
Cuts at: 0 3 1949 4441 4848
Size: 3 1946 2492 407
Fragments arranged by size:
2492 1946 407 3
NspI r_CATG'y
Cuts at: 0 1743 2469 4275 4848
Size: 1743 726 1806 573
Fragments arranged by size:
1806 1743 726 573
PasI CC'CwG_GG
Cuts at: 0 2723 4294 4668 4848
Size: 2723 1571 374 180
Fragments arranged by size:
2723 1571 374 180
PfoI T'CCnGG_A
Cuts at: 0 2729 3791 3980 4706 4848
Size: 2729 1062 189 726 142
Fragments arranged by size:
2729 1062 726 189 142
PmlI CAC'GTG
Cuts at: 0 3996 4848
Size: 3996 852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3799 3831 4848
Size: 3799 32 1017
Fragments arranged by size:
3799 1017 32
PpuMI rG'GwC_Cy
Cuts at: 0 1575 1746 1840 2727 2745 2760 2898 3198
Size: 1575 171 94 887 18 15 138 300
Cuts at: 3198 3510 4078 4848
Size: 312 568 770
Fragments arranged by size:
1575 887 770 568 312 300 171 138
94 18 15
PspOMI G'GGCC_C
Cuts at: 0 519 4409 4848
Size: 519 3890 439
Fragments arranged by size:
3890 519 439
PspXI vC'TCGA_Gb
Cuts at: 0 2740 3256 4549 4848
Size: 2740 516 1293 299
Fragments arranged by size:
2740 1293 516 299
PstI C_TGCA'G
Cuts at: 0 657 1550 3899 4848
Size: 657 893 2349 949
Fragments arranged by size:
2349 949 893 657
PvuII CAG'CTG
Cuts at: 0 2598 4020 4848
Size: 2598 1422 828
Fragments arranged by size:
2598 1422 828
SacI G_AGCT'C
Cuts at: 0 4075 4848
Size: 4075 773
SacII CC_GC'GG
Cuts at: 0 3621 3953 4848
Size: 3621 332 895
Fragments arranged by size:
3621 895 332
SanDI GG'GwC_CC
Cuts at: 0 1840 3510 4848
Size: 1840 1670 1338
Fragments arranged by size:
1840 1670 1338
SapI GCTCTTCn'nnn_
Cuts at: 0 3004 4699 4848
Size: 3004 1695 149
Fragments arranged by size:
3004 1695 149
SexAI A'CCwGG_T
Cuts at: 0 4063 4848
Size: 4063 785
SfcI C'TryA_G
Cuts at: 0 653 1546 3063 3895 4848
Size: 653 893 1517 832 953
Fragments arranged by size:
1517 953 893 832 653
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 46 4848
Size: 46 4802
SgrAI Cr'CCGG_yG
Cuts at: 0 1949 4848
Size: 1949 2899
SmaI CCC'GGG
Cuts at: 0 25 4009 4848
Size: 25 3984 839
Fragments arranged by size:
3984 839 25
SmlI C'TyrA_G
Cuts at: 0 2740 3256 4549 4848
Size: 2740 516 1293 299
Fragments arranged by size:
2740 1293 516 299
SnaBI TAC'GTA
Cuts at: 0 205 4848
Size: 205 4643
SphI G_CATG'C
Cuts at: 0 1743 4275 4848
Size: 1743 2532 573
Fragments arranged by size:
2532 1743 573
StuI AGG'CCT
Cuts at: 0 2833 3069 3262 4848
Size: 2833 236 193 1586
Fragments arranged by size:
2833 1586 236 193
StyI C'CwwG_G
Cuts at: 0 272 2531 3227 3830 4286 4349 4595 4848
Size: 272 2259 696 603 456 63 246 253
Fragments arranged by size:
2259 696 603 456 272 253 246 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 1124 2157 3094 4637 4848
Size: 774 350 1033 937 1543 211
Fragments arranged by size:
1543 1033 937 774 350 211
TatI w'GTAC_w
Cuts at: 0 1235 3221 4848
Size: 1235 1986 1627
Fragments arranged by size:
1986 1627 1235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 26 58 1590 1622 3643 3675 4848
Size: 26 32 1532 32 2021 32 1173
Fragments arranged by size:
2021 1532 1173 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 67 2284 2407 4848
Size: 67 2217 123 2441
Fragments arranged by size:
2441 2217 123 67
XbaI T'CTAG_A
Cuts at: 0 318 4848
Size: 318 4530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1863 4301 4848
Size: 1863 2438 547
Fragments arranged by size:
2438 1863 547
XhoI C'TCGA_G
Cuts at: 0 2740 3256 4549 4848
Size: 2740 516 1293 299
Fragments arranged by size:
2740 1293 516 299
XmaI C'CCGG_G
Cuts at: 0 23 4007 4848
Size: 23 3984 841
Fragments arranged by size:
3984 841 23
XmnI GAAnn'nnTTC
Cuts at: 0 1457 1630 3700 4848
Size: 1457 173 2070 1148
Fragments arranged by size:
2070 1457 1148 173
ZraI GAC'GTC
Cuts at: 0 316 4848
Size: 316 4532
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfiI SgrAI SmaI SmlI SnaBI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AscI AseI AsiSI
AvrII BbeI BcgI BciVI BfrBI BmtI BpuEI BsiWI
BsrGI BssHII BssSI BstBI ClaI CspCI EciI EcoRI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PshAI PsiI PsrI PvuI RsrII
SalI SbfI ScaI SfoI SgfI SpeI SrfI SspI
SwaI