(Circular) (Six-base) MAPSORT of: pFN21AE8000.seq Check: 7484 from: 1 to: 6007 pFN21AE8000 6007 bp With 182 enzymes: SgfI * February 29, 2012 12:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2930 2930 Size: 6007 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2779 2852 3014 3143 3197 4070 5454 5722 Size: 1147 73 162 129 54 873 1384 268 Cuts at: 5722 1632 Size: 1917 Fragments arranged by size: 1917 1384 1147 873 268 162 129 73 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 2174 2739 2751 4906 5883 1203 Size: 971 565 12 2155 977 1327 Fragments arranged by size: 2155 1327 977 971 565 12 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 2065 4736 2065 Size: 2671 3336 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2069 2416 2431 2547 2670 3235 3750 5322 2069 Size: 347 15 116 123 565 515 1572 2754 Fragments arranged by size: 2754 1572 565 515 347 123 116 15 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 2515 2978 3316 3542 3694 5803 1493 Size: 1022 463 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1022 463 338 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 3332 1870 Size: 73 1389 4545 Fragments arranged by size: 4545 1389 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 2249 3328 4783 618 Size: 325 217 627 445 17 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 445 325 217 17 BanII G_rGCy'C Cuts at: 1391 2970 3204 3326 1391 Size: 1579 234 122 4072 Fragments arranged by size: 4072 1579 234 122 BbeI G_GCGC'C Cuts at: 2253 2253 Size: 6007 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 2937 928 Size: 971 377 661 3998 Fragments arranged by size: 3998 971 661 377 BbvCI CC'TCA_GC Cuts at: 2228 2228 Size: 6007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 2337 2376 2454 1548 Size: 789 39 78 5101 Fragments arranged by size: 5101 789 78 39 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2617 2709 2820 6002 2617 Size: 92 111 3182 2622 Fragments arranged by size: 3182 2622 111 92 BlpI GC'TnA_GC Cuts at: 2537 3219 3424 2537 Size: 682 205 5120 Fragments arranged by size: 5120 682 205 Bme1580I G_kGCm'C Cuts at: 1790 2237 4068 5224 1790 Size: 447 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 447 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2079 2426 3738 Size: 416 53 83 399 24 662 347 1312 Cuts at: 3738 4696 442 Size: 958 1753 Fragments arranged by size: 1753 1312 958 662 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2333 2984 4667 1239 Size: 1094 651 1683 2579 Fragments arranged by size: 2579 1683 1094 651 Bpu10I CC'TnA_GC Cuts at: 2228 2228 Size: 6007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2008 2641 4670 882 Size: 617 509 633 2029 2219 Fragments arranged by size: 2219 2029 633 617 509 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 2453 6001 2453 Size: 3548 2459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 3814 4196 4831 Size: 53 83 186 936 717 1564 382 635 Cuts at: 4831 275 Size: 1451 Fragments arranged by size: 1564 1451 936 717 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2241 2610 4440 4838 5112 5259 5670 2241 Size: 369 1830 398 274 147 411 2578 Fragments arranged by size: 2578 1830 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2807 2837 1178 Size: 30 165 30 1404 30 4348 Fragments arranged by size: 4348 1404 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2453 3029 3032 1186 Size: 1189 78 576 3 4161 Fragments arranged by size: 4161 1189 576 78 3 BseYI C'CCAG_C Cuts at: 2686 3150 3228 5210 5706 2686 Size: 464 78 1982 496 2987 Fragments arranged by size: 2987 1982 496 464 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2444 1163 Size: 573 708 4726 Fragments arranged by size: 4726 708 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2627 3204 4068 4153 5224 2627 Size: 577 864 85 1071 3410 Fragments arranged by size: 3410 1071 864 577 85 BsmI GAATG_Cn' Cuts at: 3143 3518 3611 3143 Size: 375 93 5539 Fragments arranged by size: 5539 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2627 2970 3204 3326 4068 4153 Size: 399 447 390 343 234 122 742 85 Cuts at: 4153 5224 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 390 343 234 122 85 BspEI T'CCGG_A Cuts at: 2241 2610 5670 2241 Size: 369 3060 2578 Fragments arranged by size: 3060 2578 369 BspHI T'CATG_A Cuts at: 2169 3896 5626 2169 Size: 1727 1730 2550 Fragments arranged by size: 2550 1730 1727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 3362 844 Size: 2086 432 3489 Fragments arranged by size: 3489 2086 432 BsrBI CCG'CTC Cuts at: 1394 1823 2319 3894 1394 Size: 429 496 1575 3507 Fragments arranged by size: 3507 1575 496 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2910 4651 4838 1700 Size: 110 1100 1741 187 2869 Fragments arranged by size: 2869 1741 1100 187 110 BsrGI T'GTAC_A Cuts at: 96 2047 96 Size: 1951 4056 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 4067 5079 4067 Size: 1012 4995 BstAPI GCAn_nnn'nTGC Cuts at: 794 2547 794 Size: 1753 4254 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstYI r'GATC_y Cuts at: 1972 2617 2709 2820 3337 3704 4089 4106 5558 Size: 645 92 111 517 367 385 17 1452 Cuts at: 5558 5644 5656 6002 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 645 517 385 367 346 111 92 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 4165 Fragments arranged by size: 4165 939 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 2548 2671 2934 3523 4317 4337 2548 Size: 123 263 589 794 20 4218 Fragments arranged by size: 4218 794 589 263 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 3085 3085 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3454 4343 Size: 54 1425 50 275 18 1078 546 889 Cuts at: 4343 5972 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1078 889 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3053 3937 1353 Size: 1700 884 3423 Fragments arranged by size: 3423 1700 884 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3202 3202 Size: 6007 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2779 2852 2984 3014 3143 3197 Size: 393 701 446 73 132 30 129 54 Cuts at: 3197 4070 4667 5454 5722 1239 Size: 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 701 597 446 393 268 132 129 73 54 30 EcoNI CCTnn'n_nnAGG Cuts at: 2794 2831 3278 2794 Size: 37 447 5523 Fragments arranged by size: 5523 447 37 EcoO109I rG'GnC_Cy Cuts at: 2114 2572 2114 Size: 458 5549 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 2303 3242 4513 2303 Size: 939 1271 3797 Fragments arranged by size: 3797 1271 939 HaeII r_GCGC'y Cuts at: 1678 2253 5154 1678 Size: 575 2901 2531 Fragments arranged by size: 2901 2531 575 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2392 2424 2458 Size: 32 135 32 479 32 309 32 34 Cuts at: 2458 2490 2609 2641 2807 2839 4648 4680 4722 Size: 32 119 32 166 32 1809 32 42 Cuts at: 4722 4754 1373 Size: 32 2626 Fragments arranged by size: 2626 1809 479 309 166 135 119 42 34 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2745 2858 3351 3597 669 Size: 988 281 154 653 113 493 246 3079 Fragments arranged by size: 3079 988 653 493 281 246 154 113 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KasI G'GCGC_C Cuts at: 2249 2249 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2089 2370 5121 5305 1305 Size: 784 281 2751 184 2007 Fragments arranged by size: 2751 2007 784 281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4185 Fragments arranged by size: 4185 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2163 3188 3965 4324 4483 518 Size: 648 843 154 1025 777 359 159 2042 Fragments arranged by size: 2042 1025 843 777 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 2446 2647 3007 3097 3232 3423 4100 5248 Size: 840 201 360 90 135 191 677 1148 Cuts at: 5248 5493 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 840 677 360 245 201 191 135 90 NaeI GCC'GGC Cuts at: 2912 2912 Size: 6007 NarI GG'CG_CC Cuts at: 2250 2250 Size: 6007 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4516 Fragments arranged by size: 4516 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NgoMIV G'CCGG_C Cuts at: 2910 2910 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2178 2743 2755 2992 3365 4910 1132 Size: 97 949 565 12 237 373 1545 2229 Fragments arranged by size: 2229 1545 949 565 373 237 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6007 PciI A'CATG_T Cuts at: 2174 2739 2751 4906 2174 Size: 565 12 2155 3275 Fragments arranged by size: 3275 2155 565 12 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PfoI T'CCnGG_A Cuts at: 2074 3246 2074 Size: 1172 4835 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2114 2114 Size: 6007 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PstI C_TGCA'G Cuts at: 830 2571 2646 2941 3006 3078 3102 3359 830 Size: 1741 75 295 65 72 24 257 3478 Fragments arranged by size: 3478 1741 295 257 75 72 65 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2647 3007 3097 2647 Size: 360 90 5557 Fragments arranged by size: 5557 360 90 SacI G_AGCT'C Cuts at: 3204 3204 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2567 2642 2937 3002 3074 3098 3355 Size: 219 1522 75 295 65 72 24 257 Cuts at: 3355 4490 5171 5362 826 Size: 1135 681 191 1471 Fragments arranged by size: 1522 1471 1135 681 295 257 219 191 75 72 65 24 SfoI GGC'GCC Cuts at: 2251 2251 Size: 6007 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 3365 1132 Size: 2233 3774 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 2291 1517 Size: 774 5233 StyI C'CwwG_G Cuts at: 513 1065 2004 3446 513 Size: 552 939 1442 3074 Fragments arranged by size: 3074 1442 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3242 4034 4051 4204 4389 4834 325 Size: 1255 1662 792 17 153 185 445 1498 Fragments arranged by size: 1662 1498 1255 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2047 4253 5965 Size: 274 80 33 51 1443 70 2206 1712 Cuts at: 5965 96 Size: 138 Fragments arranged by size: 2206 1712 1443 274 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 5967 5999 2377 Size: 32 3558 32 2385 Fragments arranged by size: 3558 2385 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI