(Circular) (Six-base) MAPSORT of: pFN21AB8030.seq Check: 3688 from: 1 to: 6391 pFN21AB8030 6391 bp With 182 enzymes: SgfI * November 15, 2011 13:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3734 1937 Size: 1797 4594 Acc65I G'GTAC_C Cuts at: 3712 3712 Size: 6391 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2336 4454 5838 6106 1632 Size: 701 3 2118 1384 268 1917 Fragments arranged by size: 2118 1917 1384 701 268 3 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 3353 3664 5290 6267 1203 Size: 2150 311 1626 977 1327 Fragments arranged by size: 2150 1626 1327 977 311 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6391 AlwNI CAG_nnn'CTG Cuts at: 3390 4134 5706 3390 Size: 744 1572 4075 Fragments arranged by size: 4075 1572 744 ApaLI G'TGCA_C Cuts at: 4448 5604 4448 Size: 1156 5235 ApoI r'AATT_y Cuts at: 1493 2032 2143 2816 3253 3375 3700 3926 4078 Size: 539 111 673 437 122 325 226 152 Cuts at: 4078 6187 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 673 539 437 325 226 152 122 111 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3716 1870 Size: 73 1773 4545 Fragments arranged by size: 4545 1773 73 BamHI G'GATC_C Cuts at: 3478 3721 4088 3478 Size: 243 367 5781 Fragments arranged by size: 5781 367 243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3712 5167 618 Size: 325 217 627 1925 1455 1842 Fragments arranged by size: 1925 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3710 1391 Size: 2319 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2066 2598 3498 928 Size: 971 167 532 900 3821 Fragments arranged by size: 3821 971 900 532 167 BbvCI CC'TCA_GC Cuts at: 2152 2152 Size: 6391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3605 3639 4582 4616 1903 Size: 34 1668 34 943 34 3678 Fragments arranged by size: 3678 1668 943 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3217 4283 5493 3217 Size: 1066 1210 4115 Fragments arranged by size: 4115 1210 1066 BclI T'GATC_A Cuts at: 1548 1548 Size: 6391 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2930 5002 136 Size: 107 122 71 631 605 1258 2072 1525 Fragments arranged by size: 2072 1525 1258 631 605 122 107 71 BglII A'GATC_T Cuts at: 6386 6386 Size: 6391 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 2012 4452 5608 1790 Size: 222 2440 1156 2573 Fragments arranged by size: 2573 2440 1156 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3091 3179 3453 Size: 416 53 83 399 24 1674 88 274 Cuts at: 3453 4122 5080 442 Size: 669 958 1753 Fragments arranged by size: 1753 1674 958 669 416 399 274 88 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2421 3243 2421 Size: 822 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3660 5051 1239 Size: 2421 1391 2579 Fragments arranged by size: 2579 2421 1391 Bpu10I CC'TnA_GC Cuts at: 2152 2152 Size: 6391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5054 882 Size: 617 3555 2219 Fragments arranged by size: 3555 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4182 493 Size: 711 2978 2702 Fragments arranged by size: 2978 2702 711 BsaBI GATnn'nnATC Cuts at: 3366 6385 3366 Size: 3019 3372 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4198 4580 5215 275 Size: 53 83 186 936 2665 382 635 1451 Fragments arranged by size: 2665 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4824 5222 5496 5643 6054 4824 Size: 398 274 147 411 5161 Fragments arranged by size: 5161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2859 2889 1178 Size: 30 165 30 1456 30 4680 Fragments arranged by size: 4680 1456 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3404 1186 Size: 2218 4173 BseYI C'CCAG_C Cuts at: 2847 5594 6090 2847 Size: 2747 496 3148 Fragments arranged by size: 3148 2747 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2606 1163 Size: 573 870 4948 Fragments arranged by size: 4948 870 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3841 4602 5630 1540 Size: 275 186 1840 761 1028 2301 Fragments arranged by size: 2301 1840 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4452 4537 5608 4452 Size: 85 1071 5235 Fragments arranged by size: 5235 1071 85 BsmI GAATG_Cn' Cuts at: 3565 3902 3995 3565 Size: 337 93 5961 Fragments arranged by size: 5961 337 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3710 4452 4537 5608 1391 Size: 399 222 1698 742 85 1071 2174 Fragments arranged by size: 2174 1698 1071 742 399 222 85 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3746 844 Size: 2902 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3412 4278 1394 Size: 429 1589 866 3507 Fragments arranged by size: 3507 1589 866 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3303 4886 5060 65 Size: 1183 2055 1583 174 1396 Fragments arranged by size: 2055 1583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5035 5222 1700 Size: 110 3225 187 2869 Fragments arranged by size: 3225 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 3056 794 Size: 2262 4129 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 3346 1184 Size: 2162 4229 BstYI r'GATC_y Cuts at: 1972 3478 3721 4088 4473 4490 5942 6028 6040 Size: 1506 243 367 385 17 1452 86 12 Cuts at: 6040 6386 1972 Size: 346 1977 Fragments arranged by size: 1977 1506 1452 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5839 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2219 3463 487 Size: 683 56 993 1244 3415 Fragments arranged by size: 3415 1244 993 683 56 BtsI GCAGTG_nn' Cuts at: 3207 3907 4701 4721 3207 Size: 700 794 20 4877 Fragments arranged by size: 4877 794 700 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3455 3490 529 Size: 35 2891 35 3430 Fragments arranged by size: 3430 2891 35 35 DraI TTT'AAA Cuts at: 2611 2706 3695 2611 Size: 95 989 5307 Fragments arranged by size: 5307 989 95 DraIII CAC_nnn'GTG Cuts at: 3187 3187 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5398 809 Size: 507 4082 1802 Fragments arranged by size: 4082 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3838 4727 6356 Size: 54 1425 50 275 18 2008 889 1629 Cuts at: 6356 8 Size: 43 Fragments arranged by size: 2008 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3838 1537 Size: 275 2026 4090 Fragments arranged by size: 4090 2026 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2355 2390 2994 4321 1353 Size: 947 55 35 604 1327 3423 Fragments arranged by size: 3423 1327 947 604 55 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3301 4978 5352 5498 387 Size: 1064 138 1712 1677 374 146 1280 Fragments arranged by size: 1712 1677 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2336 3660 4454 5051 5838 6106 Size: 393 701 3 1324 794 597 787 268 Cuts at: 6106 1239 Size: 1524 Fragments arranged by size: 1524 1324 794 787 701 597 393 268 3 EcoNI CCTnn'n_nnAGG Cuts at: 2299 2299 Size: 6391 EcoRI G'AATT_C Cuts at: 2032 3253 3700 4078 2032 Size: 1221 447 378 4345 Fragments arranged by size: 4345 1221 447 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2849 3288 2849 Size: 439 5952 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 5538 1678 Size: 3860 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 5032 5064 5106 Size: 32 135 32 508 32 2920 32 42 Cuts at: 5106 5138 1373 Size: 32 2626 Fragments arranged by size: 2920 2626 508 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3735 3981 669 Size: 988 281 1797 246 3079 Fragments arranged by size: 3079 1797 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KpnI G_GTAC'C Cuts at: 3716 3716 Size: 6391 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2537 2809 5505 5689 1305 Size: 1232 272 2696 184 2007 Fragments arranged by size: 2696 2007 1232 272 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4569 Fragments arranged by size: 4569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3084 3234 4349 4708 4867 518 Size: 648 1918 150 1115 359 159 2042 Fragments arranged by size: 2042 1918 1115 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2156 3390 3654 3807 4484 5632 5877 1606 Size: 550 1234 264 153 677 1148 245 2120 Fragments arranged by size: 2120 1234 1148 677 550 264 245 153 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5839 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3357 3668 3749 5294 1132 Size: 97 2128 311 81 1545 2229 Fragments arranged by size: 2229 2128 1545 311 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 3353 3664 5290 3353 Size: 311 1626 4454 Fragments arranged by size: 4454 1626 311 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6391 PfoI T'CCnGG_A Cuts at: 3257 3257 Size: 6391 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PstI C_TGCA'G Cuts at: 830 3743 830 Size: 2913 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 2156 3390 2156 Size: 1234 5157 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SexAI A'CCwGG_T Cuts at: 3458 3458 Size: 6391 SfcI C'TryA_G Cuts at: 826 1045 3739 4874 5555 5746 826 Size: 219 2694 1135 681 191 1471 Fragments arranged by size: 2694 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3718 1872 Size: 1846 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 2760 4315 5 Size: 47 2708 1555 2081 Fragments arranged by size: 2708 2081 1555 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2121 3830 513 Size: 552 1056 1709 3074 Fragments arranged by size: 3074 1709 1056 552 SwaI ATTT'AAAT Cuts at: 2706 2706 Size: 6391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3198 3633 4418 4435 4588 4773 5218 Size: 1255 1618 435 785 17 153 185 445 Cuts at: 5218 325 Size: 1498 Fragments arranged by size: 1618 1498 1255 785 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4637 6349 96 Size: 274 80 33 51 1443 2660 1712 138 Fragments arranged by size: 2660 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3546 3578 6351 6383 3546 Size: 32 2773 32 3554 Fragments arranged by size: 3554 2773 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3497 1316 Size: 2181 4210 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 3151 2269 Size: 882 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3716 1870 Size: 1846 4545 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI