(Circular) (Six-base) MAPSORT of: pFN21AE2114.seq Check: 1915 from: 1 to: 7351 pFN21AE2114 7351 bp With 182 enzymes: SgfI * September 11, 2017 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5161 278 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2191 2304 3026 3240 4694 1937 Size: 254 113 722 214 1454 4594 Fragments arranged by size: 4594 1454 722 254 214 113 Acc65I G'GTAC_C Cuts at: 3382 4672 3382 Size: 1290 6061 AclI AA'CG_TT Cuts at: 1563 4257 5478 5851 1563 Size: 2694 1221 373 3063 Fragments arranged by size: 3063 2694 1221 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2666 4365 5414 6798 7066 1632 Size: 1034 1699 1049 1384 268 1917 Fragments arranged by size: 1917 1699 1384 1049 1034 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7154 AflIII A'CryG_T Cuts at: 1203 2115 3361 4626 6250 7227 1203 Size: 912 1246 1265 1624 977 1327 Fragments arranged by size: 1624 1327 1265 1246 977 912 AgeI A'CCGG_T Cuts at: 2186 6182 2186 Size: 3996 3355 AhdI GACnn_n'nnGTC Cuts at: 3272 6080 3272 Size: 2808 4543 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4143 1184 Size: 2959 4392 AlwNI CAG_nnn'CTG Cuts at: 3101 5094 6666 3101 Size: 1993 1572 3786 Fragments arranged by size: 3786 1993 1572 ApaI G_GGCC'C Cuts at: 2012 2012 Size: 7351 ApaLI G'TGCA_C Cuts at: 5408 6564 5408 Size: 1156 6195 ApoI r'AATT_y Cuts at: 1493 2170 2786 4122 4152 4429 4660 4886 5038 Size: 677 616 1336 30 277 231 226 152 Cuts at: 5038 7147 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1336 677 616 277 231 226 152 30 AseI AT'TA_AT Cuts at: 160 1032 3609 5905 160 Size: 872 2577 2296 1606 Fragments arranged by size: 2577 2296 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 AvaI C'yCGr_G Cuts at: 1870 1943 3636 4248 4676 1870 Size: 73 1693 612 428 4545 Fragments arranged by size: 4545 1693 612 428 73 BamHI G'GATC_C Cuts at: 2734 4681 5048 2734 Size: 1947 367 5037 Fragments arranged by size: 5037 1947 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2133 2364 3154 3382 Size: 325 217 627 338 8 231 790 228 Cuts at: 3382 3623 4672 6127 618 Size: 241 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 790 627 338 325 241 231 228 217 8 BanII G_rGCy'C Cuts at: 1391 2012 2085 2937 4224 4544 4670 1391 Size: 621 73 852 1287 320 126 4072 Fragments arranged by size: 4072 1287 852 621 320 126 73 BbeI G_GCGC'C Cuts at: 2137 2137 Size: 7351 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 928 Size: 971 562 5818 Fragments arranged by size: 5818 971 562 BbvCI CC'TCA_GC Cuts at: 2918 2918 Size: 7351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5542 5576 1903 Size: 34 3605 34 3678 Fragments arranged by size: 3678 3605 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5243 6453 5243 Size: 1210 6141 BclI T'GATC_A Cuts at: 1548 2751 3090 1548 Size: 1203 339 5809 Fragments arranged by size: 5809 1203 339 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2094 5962 136 Size: 107 122 71 631 605 422 3868 1525 Fragments arranged by size: 3868 1525 631 605 422 122 107 71 BglII A'GATC_T Cuts at: 7346 7346 Size: 7351 BlpI GC'TnA_GC Cuts at: 3642 4768 3642 Size: 1126 6225 Bme1580I G_kGCm'C Cuts at: 1790 2012 2130 2240 2367 5412 6568 1790 Size: 222 118 110 127 3045 1156 2573 Fragments arranged by size: 3045 2573 1156 222 127 118 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7351 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2479 3006 5082 Size: 416 53 83 399 24 1062 527 2076 Cuts at: 5082 6040 442 Size: 958 1753 Fragments arranged by size: 2076 1753 1062 958 527 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 3623 3705 6011 1239 Size: 923 1461 82 2306 2579 Fragments arranged by size: 2579 2306 1461 923 82 Bpu10I CC'TnA_GC Cuts at: 2918 2931 2918 Size: 13 7338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3989 4330 5476 6341 6639 3989 Size: 341 1146 865 298 4701 Fragments arranged by size: 4701 1146 865 341 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2059 3400 6014 882 Size: 617 560 1341 2614 2219 Fragments arranged by size: 2614 2219 1341 617 560 BsaAI yAC'GTr Cuts at: 493 1204 3024 3141 3364 3670 3684 5142 493 Size: 711 1820 117 223 306 14 1458 2702 Fragments arranged by size: 2702 1820 1458 711 306 223 117 14 BsaBI GATnn'nnATC Cuts at: 2750 4443 7345 2750 Size: 1693 2902 2756 Fragments arranged by size: 2902 2756 1693 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2134 4083 5158 Size: 53 83 186 936 570 31 1949 1075 Cuts at: 5158 5540 6175 275 Size: 382 635 1451 Fragments arranged by size: 1949 1451 1075 936 635 570 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 2186 3613 5784 6182 6456 6603 7014 2186 Size: 1427 2171 398 274 147 411 2523 Fragments arranged by size: 2523 2171 1427 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7126 Fragments arranged by size: 7126 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2428 4216 4543 1186 Size: 861 381 1788 327 3994 Fragments arranged by size: 3994 1788 861 381 327 BseYI C'CCAG_C Cuts at: 3223 6554 7050 3223 Size: 3331 496 3524 Fragments arranged by size: 3524 3331 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4304 1163 Size: 573 2568 4210 Fragments arranged by size: 4210 2568 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2191 4801 5562 6590 1540 Size: 275 186 190 2610 761 1028 2301 Fragments arranged by size: 2610 2301 1028 761 275 190 186 BsiHKAI G_wGCw'C Cuts at: 2830 2937 3669 4544 5412 5497 6568 2830 Size: 107 732 875 868 85 1071 3613 Fragments arranged by size: 3613 1071 875 868 732 107 85 BsmI GAATG_Cn' Cuts at: 2728 4862 4955 2728 Size: 2134 93 5124 Fragments arranged by size: 5124 2134 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2085 2130 2240 2367 2830 2937 Size: 399 222 73 45 110 127 463 107 Cuts at: 2937 3669 4224 4544 4670 5412 5497 6568 1391 Size: 732 555 320 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 732 555 463 399 320 222 127 126 110 107 85 73 45 BspEI T'CCGG_A Cuts at: 3613 7014 3613 Size: 3401 3950 BspHI T'CATG_A Cuts at: 4377 5240 6970 4377 Size: 863 1730 4758 Fragments arranged by size: 4758 1730 863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2041 4706 844 Size: 1197 2665 3489 Fragments arranged by size: 3489 2665 1197 BsrBI CCG'CTC Cuts at: 1394 1823 4208 5238 1394 Size: 429 2385 1030 3507 Fragments arranged by size: 3507 2385 1030 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 5846 6020 65 Size: 1183 1428 3170 174 1396 Fragments arranged by size: 3170 1428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2186 5995 6182 1700 Size: 110 376 3809 187 2869 Fragments arranged by size: 3809 2869 376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7351 BssSI C'ACGA_G Cuts at: 4496 5411 6423 4496 Size: 915 1012 5424 Fragments arranged by size: 5424 1012 915 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7351 BstBI TT'CG_AA Cuts at: 6155 6155 Size: 7351 BstEII G'GTnAC_C Cuts at: 1184 2758 2989 1184 Size: 1574 231 5546 Fragments arranged by size: 5546 1574 231 BstXI CCAn_nnnn'nTGG Cuts at: 3001 3601 3001 Size: 600 6751 BstYI r'GATC_y Cuts at: 1972 2289 2734 2926 4681 5048 5433 5450 6902 Size: 317 445 192 1755 367 385 17 1452 Cuts at: 6902 6988 7000 7346 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1755 1452 445 385 367 346 317 192 86 17 12 BstZ17I GTA'TAC Cuts at: 3027 3241 3027 Size: 214 7137 Bsu36I CC'TnA_GG Cuts at: 3831 3831 Size: 7351 BtgI C'CryG_G Cuts at: 513 1065 2004 3426 513 Size: 552 939 1422 4438 Fragments arranged by size: 4438 1422 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2887 487 Size: 683 56 1661 4951 Fragments arranged by size: 4951 1661 683 56 BtsI GCAGTG_nn' Cuts at: 3375 3528 3588 4867 5661 5681 3375 Size: 153 60 1279 794 20 5045 Fragments arranged by size: 5045 1279 794 153 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7351 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7316 DraI TTT'AAA Cuts at: 4655 4655 Size: 7351 DraIII CAC_nnn'GTG Cuts at: 2707 2707 Size: 7351 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6358 809 Size: 507 5042 1802 Fragments arranged by size: 5042 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2584 4798 5687 Size: 54 1425 50 275 18 754 2214 889 Cuts at: 5687 7316 8 Size: 1629 43 Fragments arranged by size: 2214 1629 1425 889 754 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4798 1537 Size: 275 2986 4090 Fragments arranged by size: 4090 2986 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2456 2699 2835 4284 5281 1353 Size: 1103 243 136 1449 997 3423 Fragments arranged by size: 3423 1449 1103 997 243 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4299 5938 6312 6458 387 Size: 1064 138 2710 1639 374 146 1280 Fragments arranged by size: 2710 1639 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2935 4542 2935 Size: 1607 5744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2666 3623 3705 4365 5414 6011 Size: 393 530 504 957 82 660 1049 597 Cuts at: 6011 6798 7066 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1049 957 787 660 597 530 504 393 268 82 EcoO109I rG'GnC_Cy Cuts at: 2009 4327 2009 Size: 2318 5033 EcoRI G'AATT_C Cuts at: 2786 4152 4660 5038 2786 Size: 1366 508 378 5099 Fragments arranged by size: 5099 1366 508 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2846 3991 2846 Size: 1145 6206 FspI TGC'GCA Cuts at: 5857 5857 Size: 7351 HaeII r_GCGC'y Cuts at: 1678 2036 2137 6498 1678 Size: 358 101 4361 2531 Fragments arranged by size: 4361 2531 358 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2139 2171 2644 2676 3115 Size: 32 135 32 567 32 473 32 439 Cuts at: 3115 3147 3258 3290 4197 4229 5992 6024 6066 Size: 32 111 32 907 32 1763 32 42 Cuts at: 6066 6098 1373 Size: 32 2626 Fragments arranged by size: 2626 1763 907 567 473 439 135 111 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2192 4695 4941 669 Size: 988 281 254 2503 246 3079 Fragments arranged by size: 3079 2503 988 281 254 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7351 HpaI GTT'AAC Cuts at: 4941 4941 Size: 7351 KasI G'GCGC_C Cuts at: 2133 2133 Size: 7351 KpnI G_GTAC'C Cuts at: 3386 4676 3386 Size: 1290 6061 MfeI C'AATT_G Cuts at: 4950 4950 Size: 7351 MluI A'CGCG_T Cuts at: 7227 7227 Size: 7351 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 2739 6465 6649 1305 Size: 1263 171 3726 184 2007 Fragments arranged by size: 3726 2007 1263 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 2586 10 Size: 54 1425 343 754 4775 Fragments arranged by size: 4775 1425 754 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 3461 3551 5309 5668 5827 518 Size: 648 1818 477 90 1758 359 159 2042 Fragments arranged by size: 2042 1818 1758 648 477 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2490 3086 4079 4318 4767 5444 6592 6837 Size: 884 596 993 239 449 677 1148 245 Cuts at: 6837 1606 Size: 2120 Fragments arranged by size: 2120 1148 993 884 677 596 449 245 239 NarI GG'CG_CC Cuts at: 2134 2134 Size: 7351 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5860 Fragments arranged by size: 5860 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7351 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7351 NotI GC'GGCC_GC Cuts at: 4798 4798 Size: 7351 NspI r_CATG'y Cuts at: 1132 1229 4630 4709 6254 1132 Size: 97 3401 79 1545 2229 Fragments arranged by size: 3401 2229 1545 97 79 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7351 PciI A'CATG_T Cuts at: 4626 6250 4626 Size: 1624 5727 PflMI CCAn_nnn'nTGG Cuts at: 1108 2299 1108 Size: 1191 6160 PfoI T'CCnGG_A Cuts at: 2833 2833 Size: 7351 PmeI GTTT'AAAC Cuts at: 4655 4655 Size: 7351 PmlI CAC'GTG Cuts at: 3670 3670 Size: 7351 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5420 5452 6933 6965 5420 Size: 32 1481 32 5806 Fragments arranged by size: 5806 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7351 PsiI TTA'TAA Cuts at: 4921 4921 Size: 7351 PspOMI G'GGCC_C Cuts at: 2008 2008 Size: 7351 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3668 3700 3668 Size: 32 7319 PstI C_TGCA'G Cuts at: 830 2052 4703 830 Size: 1222 2651 3478 Fragments arranged by size: 3478 2651 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7351 PvuII CAG'CTG Cuts at: 2490 3086 4318 2490 Size: 596 1232 5523 Fragments arranged by size: 5523 1232 596 SacI G_AGCT'C Cuts at: 2937 4544 2937 Size: 1607 5744 SalI G'TCGA_C Cuts at: 1936 2190 4693 1936 Size: 254 2503 4594 Fragments arranged by size: 4594 2503 254 SapI GCTCTTCn'nnn_ Cuts at: 2835 2835 Size: 7351 SbfI CC_TGCA'GG Cuts at: 4703 4703 Size: 7351 ScaI AGT'ACT Cuts at: 5599 7311 5599 Size: 1712 5639 SexAI A'CCwGG_T Cuts at: 2526 2526 Size: 7351 SfcI C'TryA_G Cuts at: 826 1045 2048 2323 2572 3705 4087 4699 5834 Size: 219 1003 275 249 1133 382 612 1135 Cuts at: 5834 6515 6706 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1133 1003 681 612 382 275 249 219 191 SfoI GGC'GCC Cuts at: 2135 2135 Size: 7351 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7351 SmaI CCC'GGG Cuts at: 1872 4250 4678 1872 Size: 2378 428 4545 Fragments arranged by size: 4545 2378 428 SmlI C'TyrA_G Cuts at: 820 1017 1943 4004 4309 5455 6356 6618 820 Size: 197 926 2061 305 1146 901 262 1553 Fragments arranged by size: 2061 1553 1146 926 901 305 262 197 SnaBI TAC'GTA Cuts at: 493 3024 5142 493 Size: 2531 2118 2702 Fragments arranged by size: 2702 2531 2118 SpeI A'CTAG_T Cuts at: 152 152 Size: 7351 SphI G_CATG'C Cuts at: 1132 4709 1132 Size: 3577 3774 SspI AAT'ATT Cuts at: 5 52 5275 5 Size: 47 5223 2081 Fragments arranged by size: 5223 2081 47 StuI AGG'CCT Cuts at: 1517 2266 2393 1517 Size: 749 127 6475 Fragments arranged by size: 6475 749 127 StyI C'CwwG_G Cuts at: 513 1065 2004 2012 2587 4790 513 Size: 552 939 8 575 2203 3074 Fragments arranged by size: 3074 2203 939 575 552 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2807 3021 3974 5378 5395 5548 5733 Size: 1255 1227 214 953 1404 17 153 185 Cuts at: 5733 6178 325 Size: 445 1498 Fragments arranged by size: 1498 1404 1255 1227 953 445 214 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3351 3927 5597 Size: 274 80 33 51 1443 1374 576 1670 Cuts at: 5597 7309 96 Size: 1712 138 Fragments arranged by size: 1712 1670 1443 1374 576 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 3271 3303 7311 7343 2829 Size: 32 410 32 4008 32 2837 Fragments arranged by size: 4008 2837 410 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7351 XbaI T'CTAG_A Cuts at: 4687 4687 Size: 7351 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3424 Size: 7351 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7351 XmaI C'CCGG_G Cuts at: 1870 4248 4676 1870 Size: 2378 428 4545 Fragments arranged by size: 4545 2378 428 XmnI GAAnn'nnTTC Cuts at: 2332 4519 5480 2332 Size: 2187 961 4203 Fragments arranged by size: 4203 2187 961 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5159 276 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI RsrII SacII SanDI SfiI SrfI SwaI