################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## Acc65I AccIII AgeI Aor51HI AscI Asp718I AspI AvrII BglII BlnI BsaWI BseAI BsiWI BsmBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI DrdI Eco47III EcoRI FseI KpnI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI PacI PflFI PinAI PmeI PshAI PvuI RsrII SalI ScaI SgrAI SmaI SnaBI SpeI SphI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 70 GTCTAC GT^[AC][GT]AC AclI 4734 AACGTT AA^CGTT AflII 4423 CTTAAG C^TTAAG Alw44I 1061 GTGCAC G^TGCAC ApaLI 1061 GTGCAC G^TGCAC AseI 2076 ATTAAT AT^TAAT AsnI 2076 ATTAAT AT^TAAT Asp700I 1418 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 5748 TGCGCA TGC^GCA BalI 596 TGGCCA TGG^CCA BanI 5587 GGCGCC G^G[CT][GA]CC BbeI 5587 GGCGCC GGCGC^C BbrPI 3184 CACGTG CAC^GTG BbsI 1831 GTCTTC GAAGAC(2/6) BcgI 5617 GCAATCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5781 GTATCC GTATCC(6/5) BfrI 4423 CTTAAG C^TTAAG BpuAI 1831 GTCTTC GAAGAC(2/6) BsaAI 3184 CACGTG [CT]AC^GT[GA] Bsp1407I 3802 TGTACA T^GTACA BsrGI 3802 TGTACA T^GTACA BstBI 2907 TTCGAA TT^CGAA BstEII 1907 GGTCACC G^GT[ACGT]ACC BstPI 1907 GGTCACC G^GT[ACGT]ACC BstXI 1574 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 3594 TTTAAA TTT^AAA EcoNI 6129 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1907 GGTCACC G^GT[ACGT]ACC FspI 5748 TGCGCA TGC^GCA HpaI 4477 GTTAAC GTT^AAC KasI 5587 GGCGCC G^GCGCC KspI 1903 CCGCGG CCGC^GG MluNI 596 TGGCCA TGG^CCA MscI 596 TGGCCA TGG^CCA NarI 5587 GGCGCC GG^CGCC NheI 705 GCTAGC G^CTAGC NspV 2907 TTCGAA TT^CGAA PflMI 5426 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3184 CACGTG CAC^GTG PmlI 3184 CACGTG CAC^GTG PshBI 2076 ATTAAT AT^TAAT Psp1406I 4734 AACGTT AA^CGTT SacI 3410 GAGCTC GAGCT^C SacII 1903 CCGCGG CCGC^GG SbfI 5521 CCTGCAGG CCTGCA^GG SexAI 1793 ACCTGGT A^CC[AT]GGT SfiI 1003 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 5587 GGCGCC GGC^GCC SfuI 2907 TTCGAA TT^CGAA Sse8387I 5521 CCTGCAGG CCTGCA^GG SspBI 3802 TGTACA T^GTACA Van91I 5426 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1418 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AatII 67 GACGTC GACGT^C 3301 GACGTC AccII 1904 CGCG CG^CG 4532 CGCG AocI 5194 CCTGAGG CC^T[ACGT]AGG 6133 CCTCAGG ApaI 989 GGGCCC GGGCC^C 4628 GGGCCC BbvCI 2957 CCTCAGC CCTCAGC(-5/-2) 5767 CCTCAGC BglI 1004 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5911 GCCTATCCGGC BsmI 2001 GAATGC GAATGC(1/-1) 3639 GAATGC BssSI 4179 CACGAG CACGAG(-5/-1) 5446 CTCGTG BstAPI 4536 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6312 GCAAGGCTTGC BstUI 1904 CGCG CG^CG 4532 CGCG Bsu36I 5194 CCTGAGG CC^T[ACGT]AGG 6133 CCTCAGG DraIII 4608 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 5427 CACCTTGTG Eco81I 5194 CCTGAGG CC^T[ACGT]AGG 6133 CCTCAGG EcoT22I 2778 ATGCAT ATGCA^T 4757 ATGCAT MvnI 1904 CGCG CG^CG 4532 CGCG NcoI 1436 CCATGG C^CATGG 1574 CCATGG NsiI 2778 ATGCAT ATGCA^T 4757 ATGCAT PaeR7I 978 CTCGAG C^TCGAG 1868 CTCGAG PpuMI 80 AGGACCC [GA]G^G[AT]CC[CT] 4016 AGGACCC PvuII 1033 CAGCTG CAG^CTG 2227 CAGCTG XhoI 978 CTCGAG C^TCGAG 1868 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApoI AspEI AspHI AvaI AvaII BamHI BanII BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoO109I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII