(Circular) (Six-base) MAPSORT of: pFN21AE2367.seq Check: 6139 from: 1 to: 11140 pFN21AE2367 11140 bp With 182 enzymes: SgfI * August 4, 2011 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3196 3196 Size: 11140 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2076 5310 8950 278 Size: 53 83 186 936 540 3234 3640 2468 Fragments arranged by size: 3640 3234 2468 936 540 186 83 53 AccI GT'mk_AC Cuts at: 1937 2076 8483 1937 Size: 139 6407 4594 Fragments arranged by size: 6407 4594 139 Acc65I G'GTAC_C Cuts at: 8461 8461 Size: 11140 AclI AA'CG_TT Cuts at: 1563 6740 9267 9640 1563 Size: 5177 2527 373 3063 Fragments arranged by size: 5177 3063 2527 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2267 2538 4256 4837 5183 5933 6632 Size: 434 201 271 1718 581 346 750 699 Cuts at: 6632 6645 6801 7469 9203 10587 10855 1632 Size: 13 156 668 1734 1384 268 1917 Fragments arranged by size: 1917 1734 1718 1384 750 699 668 581 434 346 271 268 201 156 13 AflII C'TTAA_G Cuts at: 820 1017 6428 820 Size: 197 5411 5532 Fragments arranged by size: 5532 5411 197 AflIII A'CryG_T Cuts at: 1203 2721 3387 3855 5190 6896 10039 11016 1203 Size: 1518 666 468 1335 1706 3143 977 1327 Fragments arranged by size: 3143 1706 1518 1335 1327 977 666 468 AgeI A'CCGG_T Cuts at: 9971 9971 Size: 11140 AhdI GACnn_n'nnGTC Cuts at: 3372 4643 5754 8274 9869 3372 Size: 1271 1111 2520 1595 4643 Fragments arranged by size: 4643 2520 1595 1271 1111 AleI CACnn'nnGTG Cuts at: 1166 5287 1166 Size: 4121 7019 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 11140 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4346 4378 4346 Size: 32 11108 AlwNI CAG_nnn'CTG Cuts at: 3893 6668 7186 8023 8041 8883 10455 3893 Size: 2775 518 837 18 842 1572 4578 Fragments arranged by size: 4578 2775 1572 842 837 518 18 ApaI G_GGCC'C Cuts at: 2998 6637 2998 Size: 3639 7501 ApaLI G'TGCA_C Cuts at: 3066 9197 10353 3066 Size: 6131 1156 3853 Fragments arranged by size: 6131 3853 1156 ApoI r'AATT_y Cuts at: 1493 2554 3788 4263 4838 4909 6574 6805 8449 Size: 1061 1234 475 575 71 1665 231 1644 Cuts at: 8449 8675 8827 10936 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1665 1644 1234 1061 575 475 231 226 152 71 AseI AT'TA_AT Cuts at: 160 1032 4082 9694 160 Size: 872 3050 5612 1606 Fragments arranged by size: 5612 3050 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11140 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2983 3873 8465 1870 Size: 73 117 923 890 4592 4545 Fragments arranged by size: 4592 4545 923 890 117 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2971 3004 3844 3877 2971 Size: 33 840 33 10234 Fragments arranged by size: 10234 840 33 33 BamHI G'GATC_C Cuts at: 2766 3692 4927 8143 8470 8837 2766 Size: 926 1235 3216 327 367 5069 Fragments arranged by size: 5069 3216 1235 926 367 327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7592 8461 9916 618 Size: 325 217 627 5805 869 1455 1842 Fragments arranged by size: 5805 1842 1455 869 627 325 217 BanII G_rGCy'C Cuts at: 1391 2998 3032 5419 6637 7221 8069 8459 1391 Size: 1607 34 2387 1218 584 848 390 4072 Fragments arranged by size: 4072 2387 1607 1218 848 584 390 34 BbeI G_GCGC'C Cuts at: 7596 7596 Size: 11140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3829 928 Size: 971 1930 8239 Fragments arranged by size: 8239 1930 971 BbvCI CC'TCA_GC Cuts at: 4963 7773 4963 Size: 2810 8330 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7611 7645 9331 9365 1903 Size: 34 5674 34 1686 34 3678 Fragments arranged by size: 5674 3678 1686 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7797 9032 10242 7797 Size: 1235 1210 8695 Fragments arranged by size: 8695 1235 1210 BclI T'GATC_A Cuts at: 1548 2481 4393 4699 6003 7104 1548 Size: 933 1912 306 1304 1101 5584 Fragments arranged by size: 5584 1912 1304 1101 933 306 BfrBI ATG'CAT Cuts at: 4785 6764 4785 Size: 1979 9161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3015 7922 9751 Size: 107 122 71 631 605 1343 4907 1829 Cuts at: 9751 136 Size: 1525 Fragments arranged by size: 4907 1829 1525 1343 631 605 122 107 71 BglII A'GATC_T Cuts at: 11135 11135 Size: 11140 BlpI GC'TnA_GC Cuts at: 2176 2921 7260 8557 2176 Size: 745 4339 1297 4759 Fragments arranged by size: 4759 4339 1297 745 Bme1580I G_kGCm'C Cuts at: 1790 2902 2998 3070 6637 8133 9201 10357 1790 Size: 1112 96 72 3567 1496 1068 1156 2573 Fragments arranged by size: 3567 2573 1496 1156 1112 1068 96 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6610 6640 8871 Size: 416 53 83 399 24 5193 30 2231 Cuts at: 8871 9829 442 Size: 958 1753 Fragments arranged by size: 5193 2231 1753 958 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 2714 1056 Size: 1658 9482 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4978 4978 Size: 11140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2617 3620 4011 4358 4373 4594 5321 Size: 942 436 1003 391 347 15 221 727 Cuts at: 5321 5978 6335 6455 6523 6977 7163 7212 7488 Size: 657 357 120 68 454 186 49 276 Cuts at: 7488 7498 7894 8177 8357 9800 1239 Size: 10 396 283 180 1443 2579 Fragments arranged by size: 2579 1443 1003 942 727 657 454 436 396 391 357 347 283 276 221 186 180 120 68 49 15 10 Bpu10I CC'TnA_GC Cuts at: 4963 7588 7773 4963 Size: 2625 185 8330 Fragments arranged by size: 8330 2625 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5424 5978 7324 9265 10130 10428 5424 Size: 554 1346 1941 865 298 6136 Fragments arranged by size: 6136 1941 1346 865 554 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2032 4839 6995 7003 8146 8282 9803 Size: 617 533 2807 2156 8 1143 136 1521 Cuts at: 9803 882 Size: 2219 Fragments arranged by size: 2807 2219 2156 1521 1143 617 533 136 8 BsaAI yAC'GTr Cuts at: 493 1204 5191 8931 493 Size: 711 3987 3740 2702 Fragments arranged by size: 3987 3740 2702 711 BsaBI GATnn'nnATC Cuts at: 2805 4398 7353 11134 2805 Size: 1593 2955 3781 2811 Fragments arranged by size: 3781 2955 2811 1593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2905 5307 5364 Size: 53 83 186 936 540 832 2402 57 Cuts at: 5364 7593 8947 9329 9964 275 Size: 2229 1354 382 635 1451 Fragments arranged by size: 2402 2229 1451 1354 936 832 635 540 382 186 83 57 53 BsaWI w'CCGG_w Cuts at: 9573 9971 10245 10392 10803 9573 Size: 398 274 147 411 9910 Fragments arranged by size: 9910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 4346 4362 4376 Size: 30 165 30 1212 30 1701 16 14 Cuts at: 4376 4392 6975 7005 8108 8138 1178 Size: 16 2583 30 1103 30 4180 Fragments arranged by size: 4180 2583 1701 1212 1103 165 30 30 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3891 5098 6622 7130 7217 7393 8180 1186 Size: 2705 1207 1524 508 87 176 787 4146 Fragments arranged by size: 4146 2705 1524 1207 787 508 176 87 BseYI C'CCAG_C Cuts at: 2999 4990 5152 6302 6718 7051 7464 7898 8208 Size: 1991 162 1150 416 333 413 434 310 Cuts at: 8208 8217 8223 8235 8241 8295 8334 10343 10839 Size: 9 6 12 6 54 39 2009 496 Cuts at: 10839 2999 Size: 3300 Fragments arranged by size: 3300 2009 1991 1150 496 434 416 413 333 310 162 54 39 12 9 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3056 3333 5113 5333 6623 8091 1163 Size: 573 1320 277 1780 220 1290 1468 4212 Fragments arranged by size: 4212 1780 1468 1320 1290 573 277 220 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3908 7926 8355 8590 9351 10379 Size: 275 186 1907 4018 429 235 761 1028 Cuts at: 10379 1540 Size: 2301 Fragments arranged by size: 4018 2301 1907 1028 761 429 275 235 186 BsiHKAI G_wGCw'C Cuts at: 3070 4283 5419 6614 9201 9286 10357 3070 Size: 1213 1136 1195 2587 85 1071 3853 Fragments arranged by size: 3853 2587 1213 1195 1136 1071 85 BsmI GAATG_Cn' Cuts at: 4012 5650 8651 8744 4012 Size: 1638 3001 93 6408 Fragments arranged by size: 6408 3001 1638 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2902 2998 3032 3070 4283 5419 6614 Size: 399 1112 96 34 38 1213 1136 1195 Cuts at: 6614 6637 7221 8069 8133 8459 9201 9286 10357 Size: 23 584 848 64 326 742 85 1071 Cuts at: 10357 1391 Size: 2174 Fragments arranged by size: 2174 1213 1195 1136 1112 1071 848 742 584 399 326 96 85 64 38 34 23 BspEI T'CCGG_A Cuts at: 10803 10803 Size: 11140 BspHI T'CATG_A Cuts at: 4165 4938 5049 9029 10759 4165 Size: 773 111 3980 1730 4546 Fragments arranged by size: 4546 3980 1730 773 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3196 3250 6431 8022 8495 844 Size: 2352 54 3181 1591 473 3489 Fragments arranged by size: 3489 3181 2352 1591 473 54 BsrBI CCG'CTC Cuts at: 1394 1823 9027 1394 Size: 429 7204 3507 Fragments arranged by size: 7204 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2696 5265 5779 6557 8292 9635 9809 Size: 1183 1448 2569 514 778 1735 1343 174 Cuts at: 9809 65 Size: 1396 Fragments arranged by size: 2569 1735 1448 1396 1343 1183 778 514 174 BsrFI r'CCGG_y Cuts at: 1700 1810 9784 9971 1700 Size: 110 7974 187 2869 Fragments arranged by size: 7974 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5807 96 Size: 5711 5429 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11140 BssSI C'ACGA_G Cuts at: 6184 7451 9200 10212 6184 Size: 1267 1749 1012 7112 Fragments arranged by size: 7112 1749 1267 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6547 8323 794 Size: 5753 1776 3611 Fragments arranged by size: 5753 3611 1776 BstBI TT'CG_AA Cuts at: 4913 9944 4913 Size: 5031 6109 BstEII G'GTnAC_C Cuts at: 1184 3912 1184 Size: 2728 8412 BstXI CCAn_nnnn'nTGG Cuts at: 3586 3586 Size: 11140 BstYI r'GATC_y Cuts at: 1972 2766 3196 3692 4927 4998 6776 8143 8470 Size: 794 430 496 1235 71 1778 1367 327 Cuts at: 8470 8837 9222 9239 10691 10777 10789 11135 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1778 1452 1367 1235 794 496 430 385 367 346 327 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 7200 8139 7200 Size: 939 10201 BtgI C'CryG_G Cuts at: 513 1065 2004 3441 3579 3908 7022 513 Size: 552 939 1437 138 329 3114 4631 Fragments arranged by size: 4631 3114 1437 939 552 329 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2658 4936 5647 6241 7614 7848 Size: 683 56 1432 2278 711 594 1373 234 Cuts at: 7848 487 Size: 3779 Fragments arranged by size: 3779 2278 1432 1373 711 683 594 234 56 BtsI GCAGTG_nn' Cuts at: 3321 3891 5969 7184 7888 8656 9450 9470 3321 Size: 570 2078 1215 704 768 794 20 4991 Fragments arranged by size: 4991 2078 1215 794 768 704 570 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11105 DraI TTT'AAA Cuts at: 5601 8444 5601 Size: 2843 8297 DraIII CAC_nnn'GTG Cuts at: 6618 7437 6618 Size: 819 10321 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10147 809 Size: 507 8831 1802 Fragments arranged by size: 8831 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2601 3905 Size: 54 1425 50 275 18 177 594 1304 Cuts at: 3905 5016 6042 7923 8352 8587 9476 11105 8 Size: 1111 1026 1881 429 235 889 1629 43 Fragments arranged by size: 1881 1629 1425 1304 1111 1026 889 594 429 275 235 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3905 7923 8352 8587 1537 Size: 275 2093 4018 429 235 4090 Fragments arranged by size: 4090 4018 2093 429 275 235 EarI CTCTTCn'nnn_ Cuts at: 1353 3599 3815 4055 4271 4856 5340 5414 6149 Size: 2246 216 240 216 585 484 74 735 Cuts at: 6149 6280 6303 6777 7093 7203 7209 7620 9070 Size: 131 23 474 316 110 6 411 1450 Cuts at: 9070 1353 Size: 3423 Fragments arranged by size: 3423 2246 1450 735 585 484 474 411 316 240 216 216 131 110 74 23 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3815 6557 9727 10101 10247 387 Size: 1064 138 2226 2742 3170 374 146 1280 Fragments arranged by size: 3170 2742 2226 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5417 5417 Size: 11140 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2181 2267 2538 2617 3620 4011 Size: 393 434 115 86 271 79 1003 391 Cuts at: 4011 4256 4358 4373 4594 4837 5183 5321 5933 Size: 245 102 15 221 243 346 138 612 Cuts at: 5933 5978 6335 6455 6523 6632 6645 6801 6977 Size: 45 357 120 68 109 13 156 176 Cuts at: 6977 7163 7212 7469 7488 7498 7894 8177 8357 Size: 186 49 257 19 10 396 283 180 Cuts at: 8357 9203 9800 10587 10855 1239 Size: 846 597 787 268 1524 Fragments arranged by size: 1524 1003 846 787 612 597 434 396 393 391 357 346 283 271 268 257 245 243 221 186 180 176 156 138 120 115 109 102 86 79 68 49 45 19 15 13 10 EcoNI CCTnn'n_nnAGG Cuts at: 8138 8138 Size: 11140 EcoO109I rG'GnC_Cy Cuts at: 2086 2994 2995 3745 6022 6634 2086 Size: 908 1 750 2277 612 6592 Fragments arranged by size: 6592 2277 908 750 612 1 EcoRI G'AATT_C Cuts at: 8449 8827 8449 Size: 378 10762 EcoRV GAT'ATC Cuts at: 2574 2585 4255 5656 2574 Size: 11 1670 1401 8058 Fragments arranged by size: 8058 1670 1401 11 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 5493 2152 Size: 3341 7799 FspI TGC'GCA Cuts at: 7755 9646 7755 Size: 1891 9249 HaeII r_GCGC'y Cuts at: 1678 2053 4672 7588 7596 10287 1678 Size: 375 2619 2916 8 2691 2531 Fragments arranged by size: 2916 2691 2619 2531 375 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3103 3135 3358 3390 3439 Size: 32 135 32 1531 32 223 32 49 Cuts at: 3439 3471 3650 3682 3824 3856 3964 3996 4629 Size: 32 179 32 142 32 108 32 633 Cuts at: 4629 4661 5740 5772 6014 6046 6830 6862 7208 Size: 32 1079 32 242 32 784 32 346 Cuts at: 7208 7240 7405 7437 8017 8049 8260 8292 9781 Size: 32 165 32 580 32 211 32 1489 Cuts at: 9781 9813 9855 9887 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 1489 1079 784 633 580 346 242 223 211 179 165 142 135 108 49 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5759 6395 6484 6673 8484 8730 Size: 988 281 3821 636 89 189 1811 246 Cuts at: 8730 669 Size: 3079 Fragments arranged by size: 3821 3079 1811 988 636 281 246 189 89 HindIII A'AGCT_T Cuts at: 748 2249 4447 7655 748 Size: 1501 2198 3208 4233 Fragments arranged by size: 4233 3208 2198 1501 HpaI GTT'AAC Cuts at: 6484 8730 6484 Size: 2246 8894 KasI G'GCGC_C Cuts at: 7592 7592 Size: 11140 KpnI G_GTAC'C Cuts at: 8465 8465 Size: 11140 MfeI C'AATT_G Cuts at: 8739 8739 Size: 11140 MluI A'CGCG_T Cuts at: 11016 11016 Size: 11140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3372 3738 4370 7738 7959 10254 10438 1305 Size: 2067 366 632 3368 221 2295 184 2007 Fragments arranged by size: 3368 2295 2067 2007 632 366 221 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2603 10 Size: 54 1425 343 771 8547 Fragments arranged by size: 8547 1425 771 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3584 3856 5073 5287 5519 9098 9457 Size: 648 2418 272 1217 214 232 3579 359 Cuts at: 9457 9616 518 Size: 159 2042 Fragments arranged by size: 3579 2418 2042 1217 648 359 272 232 214 159 MspA1I CmG'CkG Cuts at: 1606 2044 3040 3910 4234 5501 6302 7259 7582 Size: 438 996 870 324 1267 801 957 323 Cuts at: 7582 8556 9233 10381 10626 1606 Size: 974 677 1148 245 2120 Fragments arranged by size: 2120 1267 1148 996 974 957 870 801 677 438 324 323 245 NarI GG'CG_CC Cuts at: 7593 7593 Size: 11140 NcoI C'CATG_G Cuts at: 513 1065 2004 3441 3579 513 Size: 552 939 1437 138 8074 Fragments arranged by size: 8074 1437 939 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 11140 NheI G'CTAG_C Cuts at: 1052 2710 1052 Size: 1658 9482 NotI GC'GGCC_GC Cuts at: 8587 8587 Size: 11140 NsiI A_TGCA'T Cuts at: 4787 6766 4787 Size: 1979 9161 NspI r_CATG'y Cuts at: 1132 1229 2725 3859 4748 5321 5391 6679 6718 Size: 97 1496 1134 889 573 70 1288 39 Cuts at: 6718 6900 7576 8498 10043 1132 Size: 182 676 922 1545 2229 Fragments arranged by size: 2229 1545 1496 1288 1134 922 889 676 573 182 97 70 39 PasI CC'CwG_GG Cuts at: 1348 6770 1348 Size: 5422 5718 PciI A'CATG_T Cuts at: 2721 3855 6896 10039 2721 Size: 1134 3041 3143 3822 Fragments arranged by size: 3822 3143 3041 1134 PflMI CCAn_nnn'nTGG Cuts at: 1108 7437 1108 Size: 6329 4811 PfoI T'CCnGG_A Cuts at: 2262 2727 2769 3597 2262 Size: 465 42 828 9805 Fragments arranged by size: 9805 828 465 42 PmeI GTTT'AAAC Cuts at: 8444 8444 Size: 11140 PmlI CAC'GTG Cuts at: 5191 5191 Size: 11140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 9209 9241 10722 10754 4346 Size: 32 4831 32 1481 32 4732 Fragments arranged by size: 4831 4732 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 6022 2086 Size: 3936 7204 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11140 PsiI TTA'TAA Cuts at: 8710 8710 Size: 11140 PspOMI G'GGCC_C Cuts at: 2994 6633 2994 Size: 3639 7501 PspXI vC'TCGA_Gb Cuts at: 2983 2983 Size: 11140 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4849 4881 4849 Size: 32 11108 PstI C_TGCA'G Cuts at: 830 2841 3207 5871 6109 7531 7665 7800 8019 Size: 2011 366 2664 238 1422 134 135 219 Cuts at: 8019 8492 830 Size: 473 3478 Fragments arranged by size: 3478 2664 2011 1422 473 366 238 219 135 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11140 PvuII CAG'CTG Cuts at: 3040 4234 3040 Size: 1194 9946 SacI G_AGCT'C Cuts at: 5419 5419 Size: 11140 SacII CC_GC'GG Cuts at: 3911 3911 Size: 11140 SalI G'TCGA_C Cuts at: 1936 8482 1936 Size: 6546 4594 SapI GCTCTTCn'nnn_ Cuts at: 3815 4271 6303 7209 3815 Size: 456 2032 906 7746 Fragments arranged by size: 7746 2032 906 456 SbfI CC_TGCA'GG Cuts at: 7531 8492 7531 Size: 961 10179 ScaI AGT'ACT Cuts at: 9388 11100 9388 Size: 1712 9428 SexAI A'CCwGG_T Cuts at: 3798 3798 Size: 11140 SfcI C'TryA_G Cuts at: 826 1045 2837 3203 5026 5220 5867 6105 6624 Size: 219 1792 366 1823 194 647 238 519 Cuts at: 6624 6972 7527 7577 7661 7796 8015 8057 8488 Size: 348 555 50 84 135 219 42 431 Cuts at: 8488 9623 10304 10495 826 Size: 1135 681 191 1471 Fragments arranged by size: 1823 1792 1471 1135 681 647 555 519 431 366 348 238 219 219 194 191 135 84 50 42 SfiI GGCCn_nnn'nGGCC Cuts at: 3015 3015 Size: 11140 SfoI GGC'GCC Cuts at: 7594 7594 Size: 11140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11140 SmaI CCC'GGG Cuts at: 1872 8467 1872 Size: 6595 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2983 3873 5439 5957 6428 7339 Size: 197 926 1040 890 1566 518 471 911 Cuts at: 7339 9244 10145 10407 820 Size: 1905 901 262 1553 Fragments arranged by size: 1905 1566 1553 1040 926 911 901 890 518 471 262 197 SnaBI TAC'GTA Cuts at: 493 8931 493 Size: 8438 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11140 SphI G_CATG'C Cuts at: 1132 8498 1132 Size: 7366 3774 SspI AAT'ATT Cuts at: 5 52 2188 2450 2756 6070 9064 5 Size: 47 2136 262 306 3314 2994 2081 Fragments arranged by size: 3314 2994 2136 2081 306 262 47 StuI AGG'CCT Cuts at: 1517 5426 6456 8003 1517 Size: 3909 1030 1547 4654 Fragments arranged by size: 4654 3909 1547 1030 StyI C'CwwG_G Cuts at: 513 1065 2004 3138 3441 3579 4073 8272 8358 Size: 552 939 1134 303 138 494 4199 86 Cuts at: 8358 8579 513 Size: 221 3074 Fragments arranged by size: 4199 3074 1134 939 552 494 303 221 138 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3435 6629 6710 7110 9167 9184 9337 Size: 1255 1855 3194 81 400 2057 17 153 Cuts at: 9337 9522 9967 325 Size: 185 445 1498 Fragments arranged by size: 3194 2057 1855 1498 1255 445 400 185 153 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5209 5740 5807 Size: 274 80 33 51 1443 3232 531 67 Cuts at: 5807 9386 11098 96 Size: 3579 1712 138 Fragments arranged by size: 3579 3232 1712 1443 531 274 138 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2615 2647 3311 3343 11100 11132 2615 Size: 32 664 32 7757 32 2623 Fragments arranged by size: 7757 2623 664 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11140 XbaI T'CTAG_A Cuts at: 8476 8476 Size: 11140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3439 4018 5075 3439 Size: 579 1057 9504 Fragments arranged by size: 9504 1057 579 XhoI C'TCGA_G Cuts at: 1943 2983 3873 1943 Size: 1040 890 9210 Fragments arranged by size: 9210 1040 890 XmaI C'CCGG_G Cuts at: 1870 8465 1870 Size: 6595 4545 XmnI GAAnn'nnTTC Cuts at: 3427 9269 3427 Size: 5842 5298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2074 5308 8948 276 Size: 53 83 186 936 540 3234 3640 2468 Fragments arranged by size: 3640 3234 2468 936 540 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BsiWI BsmBI BstZ17I FseI FspAI NaeI NgoMIV NruI PacI RsrII SanDI SrfI SwaI