(Linear) (Six-base) MAPSORT of: KIEE2367.seq Check: 8423 from: 1 to: 6435 KIEE2367.orf With 182 enzymes: SgfI * August 4, 2011 11:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1191 6435 Size: 1191 5244 AatII G_ACGT'C Cuts at: 0 71 3305 6435 Size: 71 3234 3130 Fragments arranged by size: 3234 3130 71 AccI GT'mk_AC Cuts at: 0 71 6435 Size: 71 6364 AclI AA'CG_TT Cuts at: 0 4735 6435 Size: 4735 1700 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 262 533 2251 2832 3178 3928 4627 Size: 61 201 271 1718 581 346 750 699 Cuts at: 4627 4640 4796 5464 6435 Size: 13 156 668 971 Fragments arranged by size: 1718 971 750 699 668 581 346 271 201 156 61 13 AflII C'TTAA_G Cuts at: 0 4423 6435 Size: 4423 2012 AflIII A'CryG_T Cuts at: 0 716 1382 1850 3185 4891 6435 Size: 716 666 468 1335 1706 1544 Fragments arranged by size: 1706 1544 1335 716 666 468 AhdI GACnn_n'nnGTC Cuts at: 0 1367 2638 3749 6269 6435 Size: 1367 1271 1111 2520 166 Fragments arranged by size: 2520 1367 1271 1111 166 AleI CACnn'nnGTG Cuts at: 0 3282 6435 Size: 3282 3153 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2341 2373 6435 Size: 2341 32 4062 Fragments arranged by size: 4062 2341 32 AlwNI CAG_nnn'CTG Cuts at: 0 1888 4663 5181 6018 6036 6435 Size: 1888 2775 518 837 18 399 Fragments arranged by size: 2775 1888 837 518 399 18 ApaI G_GGCC'C Cuts at: 0 993 4632 6435 Size: 993 3639 1803 Fragments arranged by size: 3639 1803 993 ApaLI G'TGCA_C Cuts at: 0 1061 6435 Size: 1061 5374 ApoI r'AATT_y Cuts at: 0 549 1783 2258 2833 2904 4569 4800 6435 Size: 549 1234 475 575 71 1665 231 1635 Fragments arranged by size: 1665 1635 1234 575 549 475 231 71 AseI AT'TA_AT Cuts at: 0 2077 6435 Size: 2077 4358 AvaI C'yCGr_G Cuts at: 0 55 978 1868 6435 Size: 55 923 890 4567 Fragments arranged by size: 4567 923 890 55 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 966 999 1839 1872 6435 Size: 966 33 840 33 4563 Fragments arranged by size: 4563 966 840 33 33 BamHI G'GATC_C Cuts at: 0 761 1687 2922 6138 6435 Size: 761 926 1235 3216 297 Fragments arranged by size: 3216 1235 926 761 297 BanI G'GyrC_C Cuts at: 0 5587 6435 Size: 5587 848 BanII G_rGCy'C Cuts at: 0 993 1027 3414 4632 5216 6064 6435 Size: 993 34 2387 1218 584 848 371 Fragments arranged by size: 2387 1218 993 848 584 371 34 BbeI G_GCGC'C Cuts at: 0 5591 6435 Size: 5591 844 BbsI GAAGACnn'nnnn_ Cuts at: 0 1824 6435 Size: 1824 4611 BbvCI CC'TCA_GC Cuts at: 0 2958 5768 6435 Size: 2958 2810 667 Fragments arranged by size: 2958 2810 667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 5606 5640 6435 Size: 5606 34 795 Fragments arranged by size: 5606 795 34 BciVI GTATCCnnnnn_n' Cuts at: 0 5792 6435 Size: 5792 643 BclI T'GATC_A Cuts at: 0 476 2388 2694 3998 5099 6435 Size: 476 1912 306 1304 1101 1336 Fragments arranged by size: 1912 1336 1304 1101 476 306 BfrBI ATG'CAT Cuts at: 0 2780 4759 6435 Size: 2780 1979 1676 Fragments arranged by size: 2780 1979 1676 BglI GCCn_nnn'nGGC Cuts at: 0 1010 5917 6435 Size: 1010 4907 518 Fragments arranged by size: 4907 1010 518 BlpI GC'TnA_GC Cuts at: 0 171 916 5255 6435 Size: 171 745 4339 1180 Fragments arranged by size: 4339 1180 745 171 Bme1580I G_kGCm'C Cuts at: 0 897 993 1065 4632 6128 6435 Size: 897 96 72 3567 1496 307 Fragments arranged by size: 3567 1496 897 307 96 72 BmrI ACTGGGnnnn_n' Cuts at: 0 4605 4635 6435 Size: 4605 30 1800 Fragments arranged by size: 4605 1800 30 BmtI G_CTAG'C Cuts at: 0 709 6435 Size: 709 5726 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2973 6435 Size: 2973 3462 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 176 612 1615 2006 2353 2368 2589 3316 Size: 176 436 1003 391 347 15 221 727 Cuts at: 3316 3973 4330 4450 4518 4972 5158 5207 5483 Size: 657 357 120 68 454 186 49 276 Cuts at: 5483 5493 5889 6172 6352 6435 Size: 10 396 283 180 83 Fragments arranged by size: 1003 727 657 454 436 396 391 357 347 283 276 221 186 180 176 120 83 68 49 15 10 Bpu10I CC'TnA_GC Cuts at: 0 2958 5583 5768 6435 Size: 2958 2625 185 667 Fragments arranged by size: 2958 2625 667 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3419 3973 5319 6435 Size: 3419 554 1346 1116 Fragments arranged by size: 3419 1346 1116 554 BsaI GGTCTCn'nnnn_ Cuts at: 0 27 2834 4990 4998 6141 6277 6435 Size: 27 2807 2156 8 1143 136 158 Fragments arranged by size: 2807 2156 1143 158 136 27 8 BsaAI yAC'GTr Cuts at: 0 3186 6435 Size: 3186 3249 BsaBI GATnn'nnATC Cuts at: 0 800 2393 5348 6435 Size: 800 1593 2955 1087 Fragments arranged by size: 2955 1593 1087 800 BsaHI Gr'CG_yC Cuts at: 0 68 900 3302 3359 5588 6435 Size: 68 832 2402 57 2229 847 Fragments arranged by size: 2402 2229 847 832 68 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 610 640 2341 2357 2371 2387 4970 5000 Size: 610 30 1701 16 14 16 2583 30 Cuts at: 5000 6103 6133 6435 Size: 1103 30 302 Fragments arranged by size: 2583 1701 1103 610 302 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1886 3093 4617 5125 5212 5388 6175 6435 Size: 1886 1207 1524 508 87 176 787 260 Fragments arranged by size: 1886 1524 1207 787 508 260 176 87 BseYI C'CCAG_C Cuts at: 0 994 2985 3147 4297 4713 5046 5459 5893 Size: 994 1991 162 1150 416 333 413 434 Cuts at: 5893 6203 6212 6218 6230 6236 6290 6329 6435 Size: 310 9 6 12 6 54 39 106 Fragments arranged by size: 1991 1150 994 434 416 413 333 310 162 106 54 39 12 9 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1328 3108 3328 4618 6086 6435 Size: 1051 277 1780 220 1290 1468 349 Fragments arranged by size: 1780 1468 1290 1051 349 277 220 BsiEI CG_ry'CG Cuts at: 0 1903 5921 6350 6435 Size: 1903 4018 429 85 Fragments arranged by size: 4018 1903 429 85 BsiHKAI G_wGCw'C Cuts at: 0 1065 2278 3414 4609 6435 Size: 1065 1213 1136 1195 1826 Fragments arranged by size: 1826 1213 1195 1136 1065 BsmI GAATG_Cn' Cuts at: 0 2007 3645 6435 Size: 2007 1638 2790 Fragments arranged by size: 2790 2007 1638 Bsp1286I G_dGCh'C Cuts at: 0 897 993 1027 1065 2278 3414 4609 4632 Size: 897 96 34 38 1213 1136 1195 23 Cuts at: 4632 5216 6064 6128 6435 Size: 584 848 64 307 Fragments arranged by size: 1213 1195 1136 897 848 584 307 96 64 38 34 23 BspHI T'CATG_A Cuts at: 0 2160 2933 3044 6435 Size: 2160 773 111 3391 Fragments arranged by size: 3391 2160 773 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1191 1245 4426 6017 6435 Size: 1191 54 3181 1591 418 Fragments arranged by size: 3181 1591 1191 418 54 BsrDI GCAATG_nn' Cuts at: 0 691 3260 3774 4552 6287 6435 Size: 691 2569 514 778 1735 148 Fragments arranged by size: 2569 1735 778 691 514 148 BsrGI T'GTAC_A Cuts at: 0 3802 6435 Size: 3802 2633 BssSI C'ACGA_G Cuts at: 0 4179 5446 6435 Size: 4179 1267 989 Fragments arranged by size: 4179 1267 989 BstAPI GCAn_nnn'nTGC Cuts at: 0 4542 6318 6435 Size: 4542 1776 117 Fragments arranged by size: 4542 1776 117 BstBI TT'CG_AA Cuts at: 0 2908 6435 Size: 2908 3527 BstEII G'GTnAC_C Cuts at: 0 1907 6435 Size: 1907 4528 BstXI CCAn_nnnn'nTGG Cuts at: 0 1581 6435 Size: 1581 4854 BstYI r'GATC_y Cuts at: 0 761 1191 1687 2922 2993 4771 6138 6435 Size: 761 430 496 1235 71 1778 1367 297 Fragments arranged by size: 1778 1367 1235 761 496 430 297 71 Bsu36I CC'TnA_GG Cuts at: 0 5195 6134 6435 Size: 5195 939 301 Fragments arranged by size: 5195 939 301 BtgI C'CryG_G Cuts at: 0 1436 1574 1903 5017 6435 Size: 1436 138 329 3114 1418 Fragments arranged by size: 3114 1436 1418 329 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 653 2931 3642 4236 5609 5843 6435 Size: 653 2278 711 594 1373 234 592 Fragments arranged by size: 2278 1373 711 653 594 592 234 BtsI GCAGTG_nn' Cuts at: 0 1316 1886 3964 5179 5883 6435 Size: 1316 570 2078 1215 704 552 Fragments arranged by size: 2078 1316 1215 704 570 552 DraI TTT'AAA Cuts at: 0 3596 6435 Size: 3596 2839 DraIII CAC_nnn'GTG Cuts at: 0 4613 5432 6435 Size: 4613 819 1003 Fragments arranged by size: 4613 1003 819 EaeI y'GGCC_r Cuts at: 0 2 596 1900 3011 4037 5918 6347 6435 Size: 2 594 1304 1111 1026 1881 429 88 Fragments arranged by size: 1881 1304 1111 1026 594 429 88 2 EagI C'GGCC_G Cuts at: 0 1900 5918 6347 6435 Size: 1900 4018 429 88 Fragments arranged by size: 4018 1900 429 88 EarI CTCTTCn'nnn_ Cuts at: 0 1594 1810 2050 2266 2851 3335 3409 4144 Size: 1594 216 240 216 585 484 74 735 Cuts at: 4144 4275 4298 4772 5088 5198 5204 5615 6435 Size: 131 23 474 316 110 6 411 820 Fragments arranged by size: 1594 820 735 585 484 474 411 316 240 216 216 131 110 74 23 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1810 4552 6435 Size: 1810 2742 1883 Fragments arranged by size: 2742 1883 1810 EcoICRI GAG'CTC Cuts at: 0 3412 6435 Size: 3412 3023 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 176 262 533 612 1615 2006 2251 Size: 61 115 86 271 79 1003 391 245 Cuts at: 2251 2353 2368 2589 2832 3178 3316 3928 3973 Size: 102 15 221 243 346 138 612 45 Cuts at: 3973 4330 4450 4518 4627 4640 4796 4972 5158 Size: 357 120 68 109 13 156 176 186 Cuts at: 5158 5207 5464 5483 5493 5889 6172 6352 6435 Size: 49 257 19 10 396 283 180 83 Fragments arranged by size: 1003 612 396 391 357 346 283 271 257 245 243 221 186 180 176 156 138 120 115 109 102 86 83 79 68 61 49 45 19 15 13 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 6133 6435 Size: 6133 302 EcoO109I rG'GnC_Cy Cuts at: 0 81 989 990 1740 4017 4629 6435 Size: 81 908 1 750 2277 612 1806 Fragments arranged by size: 2277 1806 908 750 612 81 1 EcoRV GAT'ATC Cuts at: 0 569 580 2250 3651 6435 Size: 569 11 1670 1401 2784 Fragments arranged by size: 2784 1670 1401 569 11 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 3488 6435 Size: 147 3341 2947 Fragments arranged by size: 3341 2947 147 FspI TGC'GCA Cuts at: 0 5750 6435 Size: 5750 685 HaeII r_GCGC'y Cuts at: 0 48 2667 5583 5591 6435 Size: 48 2619 2916 8 844 Fragments arranged by size: 2916 2619 844 48 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1098 1130 1353 1385 1434 1466 1645 1677 Size: 1098 32 223 32 49 32 179 32 Cuts at: 1677 1819 1851 1959 1991 2624 2656 3735 3767 Size: 142 32 108 32 633 32 1079 32 Cuts at: 3767 4009 4041 4825 4857 5203 5235 5400 5432 Size: 242 32 784 32 346 32 165 32 Cuts at: 5432 6012 6044 6255 6287 6435 Size: 580 32 211 32 148 Fragments arranged by size: 1098 1079 784 633 580 346 242 223 211 179 165 148 142 108 49 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 3754 4390 4479 4668 6435 Size: 3754 636 89 189 1767 Fragments arranged by size: 3754 1767 636 189 89 HindIII A'AGCT_T Cuts at: 0 244 2442 5650 6435 Size: 244 2198 3208 785 Fragments arranged by size: 3208 2198 785 244 HpaI GTT'AAC Cuts at: 0 4479 6435 Size: 4479 1956 KasI G'GCGC_C Cuts at: 0 5587 6435 Size: 5587 848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1367 1733 2365 5733 5954 6435 Size: 1367 366 632 3368 221 481 Fragments arranged by size: 3368 1367 632 481 366 221 MscI TGG'CCA Cuts at: 0 598 6435 Size: 598 5837 MslI CAynn'nnrTG Cuts at: 0 1579 1851 3068 3282 3514 6435 Size: 1579 272 1217 214 232 2921 Fragments arranged by size: 2921 1579 1217 272 232 214 MspA1I CmG'CkG Cuts at: 0 39 1035 1905 2229 3496 4297 5254 5577 Size: 39 996 870 324 1267 801 957 323 Cuts at: 5577 6435 Size: 858 Fragments arranged by size: 1267 996 957 870 858 801 324 323 39 NarI GG'CG_CC Cuts at: 0 5588 6435 Size: 5588 847 NcoI C'CATG_G Cuts at: 0 1436 1574 6435 Size: 1436 138 4861 Fragments arranged by size: 4861 1436 138 NheI G'CTAG_C Cuts at: 0 705 6435 Size: 705 5730 NsiI A_TGCA'T Cuts at: 0 2782 4761 6435 Size: 2782 1979 1674 Fragments arranged by size: 2782 1979 1674 NspI r_CATG'y Cuts at: 0 720 1854 2743 3316 3386 4674 4713 4895 Size: 720 1134 889 573 70 1288 39 182 Cuts at: 4895 5571 6435 Size: 676 864 Fragments arranged by size: 1288 1134 889 864 720 676 573 182 70 39 PasI CC'CwG_GG Cuts at: 0 4765 6435 Size: 4765 1670 PciI A'CATG_T Cuts at: 0 716 1850 4891 6435 Size: 716 1134 3041 1544 Fragments arranged by size: 3041 1544 1134 716 PflMI CCAn_nnn'nTGG Cuts at: 0 5432 6435 Size: 5432 1003 PfoI T'CCnGG_A Cuts at: 0 257 722 764 1592 6435 Size: 257 465 42 828 4843 Fragments arranged by size: 4843 828 465 257 42 PmlI CAC'GTG Cuts at: 0 3186 6435 Size: 3186 3249 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2341 2373 6435 Size: 2341 32 4062 Fragments arranged by size: 4062 2341 32 PpuMI rG'GwC_Cy Cuts at: 0 81 4017 6435 Size: 81 3936 2418 Fragments arranged by size: 3936 2418 81 PspOMI G'GGCC_C Cuts at: 0 989 4628 6435 Size: 989 3639 1807 Fragments arranged by size: 3639 1807 989 PspXI vC'TCGA_Gb Cuts at: 0 978 6435 Size: 978 5457 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2844 2876 6435 Size: 2844 32 3559 Fragments arranged by size: 3559 2844 32 PstI C_TGCA'G Cuts at: 0 836 1202 3866 4104 5526 5660 5795 6014 Size: 836 366 2664 238 1422 134 135 219 Cuts at: 6014 6435 Size: 421 Fragments arranged by size: 2664 1422 836 421 366 238 219 135 134 PvuII CAG'CTG Cuts at: 0 1035 2229 6435 Size: 1035 1194 4206 Fragments arranged by size: 4206 1194 1035 SacI G_AGCT'C Cuts at: 0 3414 6435 Size: 3414 3021 SacII CC_GC'GG Cuts at: 0 1906 6435 Size: 1906 4529 SapI GCTCTTCn'nnn_ Cuts at: 0 1810 2266 4298 5204 6435 Size: 1810 456 2032 906 1231 Fragments arranged by size: 2032 1810 1231 906 456 SbfI CC_TGCA'GG Cuts at: 0 5526 6435 Size: 5526 909 SexAI A'CCwGG_T Cuts at: 0 1793 6435 Size: 1793 4642 SfcI C'TryA_G Cuts at: 0 832 1198 3021 3215 3862 4100 4619 4967 Size: 832 366 1823 194 647 238 519 348 Cuts at: 4967 5522 5572 5656 5791 6010 6052 6435 Size: 555 50 84 135 219 42 383 Fragments arranged by size: 1823 832 647 555 519 383 366 348 238 219 194 135 84 50 42 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1010 6435 Size: 1010 5425 SfoI GGC'GCC Cuts at: 0 5589 6435 Size: 5589 846 SmlI C'TyrA_G Cuts at: 0 978 1868 3434 3952 4423 5334 6435 Size: 978 890 1566 518 471 911 1101 Fragments arranged by size: 1566 1101 978 911 890 518 471 SspI AAT'ATT Cuts at: 0 183 445 751 4065 6435 Size: 183 262 306 3314 2370 Fragments arranged by size: 3314 2370 306 262 183 StuI AGG'CCT Cuts at: 0 3421 4451 5998 6435 Size: 3421 1030 1547 437 Fragments arranged by size: 3421 1547 1030 437 StyI C'CwwG_G Cuts at: 0 1133 1436 1574 2068 6267 6353 6435 Size: 1133 303 138 494 4199 86 82 Fragments arranged by size: 4199 1133 494 303 138 86 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1430 4624 4705 5105 6435 Size: 1430 3194 81 400 1330 Fragments arranged by size: 3194 1430 1330 400 81 TatI w'GTAC_w Cuts at: 0 3204 3735 3802 6435 Size: 3204 531 67 2633 Fragments arranged by size: 3204 2633 531 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 610 642 1306 1338 6435 Size: 610 32 664 32 5097 Fragments arranged by size: 5097 664 610 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1434 2013 3070 6435 Size: 1434 579 1057 3365 Fragments arranged by size: 3365 1434 1057 579 XhoI C'TCGA_G Cuts at: 0 978 1868 6435 Size: 978 890 4567 Fragments arranged by size: 4567 978 890 XmnI GAAnn'nnTTC Cuts at: 0 1422 6435 Size: 1422 5013 ZraI GAC'GTC Cuts at: 0 69 3303 6435 Size: 69 3234 3132 Fragments arranged by size: 3234 3132 69 Enzymes that do cut: AarI AatII AccI AclI AcuI AflII AflIII AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI MslI MspA1I NarI NcoI NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: Acc65I AfeI AgeI AlfI AscI AsiSI AvrII BglII BmgBI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstZ17I ClaI CspCI DrdI EcoRI FseI FspAI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI PacI PmeI PshAI PsiI PvuI RsrII SalI SanDI ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmaI