(Circular) (Six-base) MAPSORT of: pFN21AE4224.seq Check: 267 from: 1 to: 7063 pFN21AE4224 7063 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3770 3770 Size: 7063 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4873 278 Size: 53 83 186 936 3337 2468 Fragments arranged by size: 3337 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3843 4406 1937 Size: 1906 563 4594 Fragments arranged by size: 4594 1906 563 Acc65I G'GTAC_C Cuts at: 4384 4384 Size: 7063 AclI AA'CG_TT Cuts at: 1563 5190 5563 1563 Size: 3627 373 3063 Fragments arranged by size: 3627 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5126 6510 6778 1632 Size: 3494 1384 268 1917 Fragments arranged by size: 3494 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6866 AflIII A'CryG_T Cuts at: 1203 5962 6939 1203 Size: 4759 977 1327 Fragments arranged by size: 4759 1327 977 AgeI A'CCGG_T Cuts at: 5894 5894 Size: 7063 AhdI GACnn_n'nnGTC Cuts at: 2157 5792 2157 Size: 3635 3428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7063 AlwNI CAG_nnn'CTG Cuts at: 2020 2034 3586 4806 6378 2020 Size: 14 1552 1220 1572 2705 Fragments arranged by size: 2705 1572 1552 1220 14 ApaLI G'TGCA_C Cuts at: 5120 6276 5120 Size: 1156 5907 ApoI r'AATT_y Cuts at: 1493 2270 2278 2357 2452 3340 4372 4598 4750 Size: 777 8 79 95 888 1032 226 152 Cuts at: 4750 6859 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1032 888 777 226 152 95 79 8 AseI AT'TA_AT Cuts at: 160 1032 5617 160 Size: 872 4585 1606 Fragments arranged by size: 4585 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2540 4124 4203 4388 1870 Size: 73 147 450 1584 79 185 4545 Fragments arranged by size: 4545 1584 450 185 147 79 73 BamHI G'GATC_C Cuts at: 2067 4393 4760 2067 Size: 2326 367 4370 Fragments arranged by size: 4370 2326 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4384 5839 618 Size: 325 217 627 2597 1455 1842 Fragments arranged by size: 2597 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2751 3759 4382 1391 Size: 730 630 1008 623 4072 Fragments arranged by size: 4072 1008 730 630 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3368 3392 928 Size: 971 1469 24 4599 Fragments arranged by size: 4599 1469 971 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5254 5288 1903 Size: 34 3317 34 3678 Fragments arranged by size: 3678 3317 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4224 4955 6165 4224 Size: 731 1210 5122 Fragments arranged by size: 5122 1210 731 BclI T'GATC_A Cuts at: 1548 1548 Size: 7063 BfrBI ATG'CAT Cuts at: 2250 3210 2250 Size: 960 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3796 5674 136 Size: 107 122 71 631 605 2124 1878 1525 Fragments arranged by size: 2124 1878 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2927 7058 2927 Size: 4131 2932 BlpI GC'TnA_GC Cuts at: 4480 4480 Size: 7063 Bme1580I G_kGCm'C Cuts at: 1790 5124 6280 1790 Size: 3334 1156 2573 Fragments arranged by size: 3334 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7063 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4027 4794 5752 Size: 416 53 83 399 24 2610 767 958 Cuts at: 5752 442 Size: 1753 Fragments arranged by size: 2610 1753 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7063 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2315 2952 3356 3878 4270 5723 1239 Size: 803 273 637 404 522 392 1453 2579 Fragments arranged by size: 2579 1453 803 637 522 404 392 273 Bpu10I CC'TnA_GC Cuts at: 3753 3753 Size: 7063 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5188 6053 6351 5188 Size: 865 298 5900 Fragments arranged by size: 5900 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5726 882 Size: 617 4227 2219 Fragments arranged by size: 4227 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2267 4854 493 Size: 711 1063 2587 2702 Fragments arranged by size: 2702 2587 1063 711 BsaBI GATnn'nnATC Cuts at: 7057 7057 Size: 7063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3731 4870 5252 5887 Size: 53 83 186 936 2198 1139 382 635 Cuts at: 5887 275 Size: 1451 Fragments arranged by size: 2198 1451 1139 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5496 5894 6168 6315 6726 5496 Size: 398 274 147 411 5833 Fragments arranged by size: 5833 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6838 Fragments arranged by size: 6838 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3409 4140 1186 Size: 2223 731 4109 Fragments arranged by size: 4109 2223 731 BseYI C'CCAG_C Cuts at: 2172 6266 6762 2172 Size: 4094 496 2473 Fragments arranged by size: 4094 2473 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4049 4513 5274 6302 1540 Size: 275 186 2048 464 761 1028 2301 Fragments arranged by size: 2301 2048 1028 761 464 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2751 2925 5124 5209 6280 2121 Size: 630 174 2199 85 1071 2904 Fragments arranged by size: 2904 2199 1071 630 174 85 BsmI GAATG_Cn' Cuts at: 4574 4667 4574 Size: 93 6970 BsmBI CGTCTCn'nnnn_ Cuts at: 3942 3942 Size: 7063 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2751 2925 3759 4382 5124 5209 Size: 399 331 630 174 834 623 742 85 Cuts at: 5209 6280 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 834 742 630 623 399 331 174 85 BspEI T'CCGG_A Cuts at: 6726 6726 Size: 7063 BspHI T'CATG_A Cuts at: 2751 3414 4952 6682 2751 Size: 663 1538 1730 3132 Fragments arranged by size: 3132 1730 1538 663 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3770 4418 844 Size: 2926 648 3489 Fragments arranged by size: 3489 2926 648 BsrBI CCG'CTC Cuts at: 1394 1823 4950 1394 Size: 429 3127 3507 Fragments arranged by size: 3507 3127 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3328 5558 5732 65 Size: 1183 2080 2230 174 1396 Fragments arranged by size: 2230 2080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2514 5707 5894 1700 Size: 110 704 3193 187 2869 Fragments arranged by size: 3193 2869 704 187 110 BsrGI T'GTAC_A Cuts at: 96 3252 4276 96 Size: 3156 1024 2883 Fragments arranged by size: 3156 2883 1024 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7063 BssSI C'ACGA_G Cuts at: 3228 3474 5123 6135 3228 Size: 246 1649 1012 4156 Fragments arranged by size: 4156 1649 1012 246 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7063 BstBI TT'CG_AA Cuts at: 5867 5867 Size: 7063 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7063 BstXI CCAn_nnnn'nTGG Cuts at: 3916 3916 Size: 7063 BstYI r'GATC_y Cuts at: 1972 2067 2927 4393 4760 5145 5162 6614 6700 Size: 95 860 1466 367 385 17 1452 86 Cuts at: 6700 6712 7058 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1466 1452 860 385 367 346 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2051 3834 513 Size: 552 939 47 1783 3742 Fragments arranged by size: 3742 1783 939 552 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2128 487 Size: 683 56 902 5422 Fragments arranged by size: 5422 902 683 56 BtsI GCAGTG_nn' Cuts at: 3095 4579 5373 5393 3095 Size: 1484 794 20 4765 Fragments arranged by size: 4765 1484 794 20 ClaI AT'CG_AT Cuts at: 1554 2716 1554 Size: 1162 5901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7028 DraI TTT'AAA Cuts at: 4367 4367 Size: 7063 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4048 6070 809 Size: 507 2732 2022 1802 Fragments arranged by size: 2732 2022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3837 4510 5399 Size: 54 1425 50 275 18 2007 673 889 Cuts at: 5399 7028 8 Size: 1629 43 Fragments arranged by size: 2007 1629 1425 889 673 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4510 1537 Size: 275 2698 4090 Fragments arranged by size: 4090 2698 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 3069 3800 4993 1353 Size: 1386 330 731 1193 3423 Fragments arranged by size: 3423 1386 1193 731 330 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5650 6024 6170 387 Size: 1064 138 4061 374 146 1280 Fragments arranged by size: 4061 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2749 2119 Size: 630 6433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2315 2952 3356 3878 4270 5126 Size: 393 410 273 637 404 522 392 856 Cuts at: 5126 5723 6510 6778 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 856 787 637 597 522 410 404 393 392 273 268 EcoRI G'AATT_C Cuts at: 3340 4372 4750 3340 Size: 1032 378 5653 Fragments arranged by size: 5653 1032 378 EcoRV GAT'ATC Cuts at: 2497 2497 Size: 7063 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2390 2390 Size: 7063 FspI TGC'GCA Cuts at: 5569 5569 Size: 7063 HaeII r_GCGC'y Cuts at: 1678 6210 1678 Size: 4532 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2059 2091 2143 2175 2912 Size: 32 135 32 487 32 52 32 737 Cuts at: 2912 2944 4007 4039 5704 5736 5778 5810 1373 Size: 32 1063 32 1665 32 42 32 2626 Fragments arranged by size: 2626 1665 1063 737 487 135 52 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4407 4653 669 Size: 988 281 2469 246 3079 Fragments arranged by size: 3079 2469 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7063 HpaI GTT'AAC Cuts at: 4653 4653 Size: 7063 KpnI G_GTAC'C Cuts at: 4388 4388 Size: 7063 MfeI C'AATT_G Cuts at: 4662 4662 Size: 7063 MluI A'CGCG_T Cuts at: 6939 6939 Size: 7063 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3075 3156 3513 4106 4122 4175 6177 6361 Size: 1770 81 357 593 16 53 2002 184 Cuts at: 6361 1305 Size: 2007 Fragments arranged by size: 2007 2002 1770 593 357 184 81 53 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5241 Fragments arranged by size: 5241 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2526 5021 5380 5539 518 Size: 648 1360 2495 359 159 2042 Fragments arranged by size: 2495 2042 1360 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 3953 4106 4479 5156 6304 6549 1606 Size: 570 1777 153 373 677 1148 245 2120 Fragments arranged by size: 2120 1777 1148 677 570 373 245 153 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5572 Fragments arranged by size: 5572 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7063 NotI GC'GGCC_GC Cuts at: 4510 4510 Size: 7063 NsiI A_TGCA'T Cuts at: 2252 3212 2252 Size: 960 6103 NspI r_CATG'y Cuts at: 1132 1229 2881 3321 4421 5966 1132 Size: 97 1652 440 1100 1545 2229 Fragments arranged by size: 2229 1652 1545 1100 440 97 PasI CC'CwG_GG Cuts at: 1348 3959 1348 Size: 2611 4452 PciI A'CATG_T Cuts at: 5962 5962 Size: 7063 PflMI CCAn_nnn'nTGG Cuts at: 1108 3496 3991 1108 Size: 2388 495 4180 Fragments arranged by size: 4180 2388 495 PfoI T'CCnGG_A Cuts at: 2058 2826 3967 2058 Size: 768 1141 5154 Fragments arranged by size: 5154 1141 768 PmeI GTTT'AAAC Cuts at: 4367 4367 Size: 7063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2523 2555 5132 5164 6645 6677 2523 Size: 32 2577 32 1481 32 2909 Fragments arranged by size: 2909 2577 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7063 PsiI TTA'TAA Cuts at: 4633 4633 Size: 7063 PspXI vC'TCGA_Gb Cuts at: 4203 4203 Size: 7063 PstI C_TGCA'G Cuts at: 830 2016 2562 3349 4415 830 Size: 1186 546 787 1066 3478 Fragments arranged by size: 3478 1186 1066 787 546 PvuI CG_AT'CG Cuts at: 2001 4049 2001 Size: 2048 5015 PvuII CAG'CTG Cuts at: 3953 4106 3953 Size: 153 6910 SacI G_AGCT'C Cuts at: 2121 2751 2121 Size: 630 6433 SalI G'TCGA_C Cuts at: 1936 4405 1936 Size: 2469 4594 SapI GCTCTTCn'nnn_ Cuts at: 2739 2739 Size: 7063 SbfI CC_TGCA'GG Cuts at: 4415 4415 Size: 7063 ScaI AGT'ACT Cuts at: 5311 7023 5311 Size: 1712 5351 SfcI C'TryA_G Cuts at: 826 1045 2012 2558 2687 3345 3948 4161 4411 Size: 219 967 546 129 658 603 213 250 Cuts at: 4411 5546 6227 6418 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 967 681 658 603 546 250 219 213 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7063 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7063 SmaI CCC'GGG Cuts at: 1872 4126 4390 1872 Size: 2254 264 4545 Fragments arranged by size: 4545 2254 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 2540 4203 5167 6068 6330 Size: 197 926 147 450 1663 964 901 262 Cuts at: 6330 820 Size: 1553 Fragments arranged by size: 1663 1553 964 926 901 450 262 197 147 SnaBI TAC'GTA Cuts at: 493 4854 493 Size: 4361 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7063 SphI G_CATG'C Cuts at: 1132 4421 1132 Size: 3289 3774 SspI AAT'ATT Cuts at: 5 52 3685 4987 5 Size: 47 3633 1302 2081 Fragments arranged by size: 3633 2081 1302 47 StuI AGG'CCT Cuts at: 1517 2839 1517 Size: 1322 5741 StyI C'CwwG_G Cuts at: 513 1065 2004 2349 2775 3244 3794 3891 4502 Size: 552 939 345 426 469 550 97 611 Cuts at: 4502 513 Size: 3074 Fragments arranged by size: 3074 939 611 552 550 469 426 345 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 5090 5107 5260 5445 5890 325 Size: 1255 1515 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1515 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 4276 5309 Size: 274 80 33 51 1443 1275 1024 1033 Cuts at: 5309 7021 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1275 1033 1024 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7023 7055 7023 Size: 32 7031 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7063 XbaI T'CTAG_A Cuts at: 4399 4399 Size: 7063 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3832 3832 Size: 7063 XhoI C'TCGA_G Cuts at: 1943 2090 2540 4203 1943 Size: 147 450 1663 4803 Fragments arranged by size: 4803 1663 450 147 XmaI C'CCGG_G Cuts at: 1870 4124 4388 1870 Size: 2254 264 4545 Fragments arranged by size: 4545 2254 264 XmnI GAAnn'nnTTC Cuts at: 5192 5192 Size: 7063 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4871 276 Size: 53 83 186 936 3337 2468 Fragments arranged by size: 3337 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI