################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII CfoI ClaI CpoI DraII DraIII DrdI Eam1105I Eco47III EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MunI NarI NciI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 396 GACGTC GACGT^C AcsI 286 GAATTT [GA]^AATT[CT] AcyI 396 GACGTC G[GA]^CG[CT]C AlwNI 587 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 45 CCTGAGG CC^T[ACGT]AGG ApoI 286 GAATTT [GA]^AATT[CT] AseI 268 ATTAAT AT^TAAT AsnI 268 ATTAAT AT^TAAT AvaI 52 CTCGGG C^[CT]CG[GA]G BamHI 199 GGATCC G^GATCC BcgI 375 GCAGCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 396 GACGTC G[GA]^CG[CT]C BseRI 659 CTCCTC GAGGAG(10/8) BsiEI 73 CGGCCG CG[GA][CT]^CG BsmFI 7 GGGAC GGGAC(10/14) BsmI 314 GCATTC GAATGC(1/-1) BsoBI 52 CTCGGG C^[CT]CG[GA]G BsrDI 40 CATTGC GCAATG(2/0) BsrFI 722 GCCGGC [GA]^CCGG[CT] BstF5I 510 GGATG GGATG(2/0) BstYI 199 GGATCC [GA]^GATC[CT] Bsu36I 45 CCTGAGG CC^T[ACGT]AGG Cfr10I 722 GCCGGC [GA]^CCGG[CT] DpnII 200 GATC ^GATC DraI 109 TTTAAA TTT^AAA DsaI 76 CCGCGG C^C[GA][CT]GG EagI 73 CGGCCG C^GGCCG EclXI 73 CGGCCG C^GGCCG Eco52I 73 CGGCCG C^GGCCG Eco81I 45 CCTGAGG CC^T[ACGT]AGG FokI 510 GGATG GGATG(9/13) HapII 723 CCGG C^CGG Hin1I 396 GACGTC G[GA]^CG[CT]C HincII 541 GTTGAC GT[CT]^[GA]AC HindII 541 GTTGAC GT[CT]^[GA]AC HpaII 723 CCGG C^CGG KspI 76 CCGCGG CCGC^GG MaeII 397 ACGT A^CGT MboI 200 GATC ^GATC MflI 199 GGATCC [GA]^GATC[CT] MspI 723 CCGG C^CGG NaeI 722 GCCGGC GCC^GGC NdeII 200 GATC ^GATC NgoMIV 722 GCCGGC G^CCGGC NotI 72 GCGGCCGC GC^GGCCGC PshBI 268 ATTAAT AT^TAAT SacII 76 CCGCGG CCGC^GG Sau3AI 200 GATC ^GATC SfaNI 118 GCATC GCATC(5/9) SfcI 122 CTATAG C^T[GA][CT]AG StuI 697 AGGCCT AGG^CCT TaqI 65 TCGA T^CGA TthHB8I 65 TCGA T^CGA XhoII 199 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 31 CGCG CG^CG 77 CGCG AlwI 200 GATCC GGATC(4/5) 199 GGATC AspHI 437 GAGCTC G[AT]GC[AT]^C 597 GAGCTC BanII 437 GAGCTC G[GA]GC[CT]^C 597 GAGCTC BbvCI 337 GCTGAGG CCTCAGC(-5/-2) 528 GCTGAGG BmyI 437 GAGCTC G[AGT]GC[ACT]^C 597 GAGCTC BpmI 452 CTCCAG CTGGAG(16/14) 624 CTCCAG BsaJI 76 CCGCGG C^C[ACGT][ACGT]GG 195 CCCTGG BsiHKAI 437 GAGCTC G[AT]GC[AT]^C 597 GAGCTC BsiYI 18 CCCGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 451 CCTCCAGTTGG BslI 18 CCCGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 451 CCTCCAGTTGG Bsp1286I 437 GAGCTC G[AGT]GC[ACT]^C 597 GAGCTC BspHI 273 TCATGA T^CATGA 746 TCATGA BsrI 280 ACTGG ACTGG(1/-1) 454 CCAGT BssKI 196 CCTGG ^CC[ACGT]GG 357 CCAGG BstNI 196 CCTGG CC^[AT]GG 357 CCAGG BstUI 31 CGCG CG^CG 77 CGCG EaeI 73 CGGCCG [CT]^GGCC[GA] 724 CGGCCA EarI 582 CTCTTC CTCTTC(1/4) 600 CTCTTC EcoRII 196 CCTGG ^CC[AT]GG 357 CCAGG FauI 18 CCCGC CCCGC(4/6) 78 GCGGG HinfI 589 GACTC G^A[ACGT]TC 781 GAGTC Ksp632I 582 CTCTTC CTCTTC(1/4) 600 CTCTTC MseI 110 TTAA T^TAA 269 TTAA MslI 213 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 315 CATTCAGGTG MspA1I 19 CCGCTG C[AC]G^C[GT]G 76 CCGCGG MvaI 196 CCTGG CC^[AT]GG 357 CCAGG MvnI 31 CGCG CG^CG 77 CGCG MwoI 569 GCAGTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 629 GCAGTGGAGGC PleI 589 GACTC GAGTC(4/5) 781 GAGTC RcaI 273 TCATGA T^CATGA 746 TCATGA SacI 437 GAGCTC GAGCT^C 597 GAGCTC SapI 581 GCTCTTC GCTCTTC(1/4) 599 GCTCTTC ScrFI 196 CCTGG CC^[ACGT]GG 357 CCAGG Tru9I 110 TTAA T^TAA 269 TTAA Tsp509I 177 AATT ^AATT 287 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI AvaII BbvI Cac8I Cfr13I DdeI Fnu4HI HaeIII HphI ItaI MaeIII MboII MnlI NlaIII NlaIV Sau96I TseI Tsp45I TspRI