(Circular) (Six-base) MAPSORT of: pFN21AE9275.seq Check: 3344 from: 1 to: 5509 pFN21AE9275 5509 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2405 3319 278 Size: 53 83 186 936 869 914 2468 Fragments arranged by size: 2468 936 914 869 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 2574 3572 4956 5224 1632 Size: 930 12 998 1384 268 1917 Fragments arranged by size: 1917 1384 998 930 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 4408 5385 1203 Size: 3205 977 1327 Fragments arranged by size: 3205 1327 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 2597 3252 4824 2597 Size: 655 1572 3282 Fragments arranged by size: 3282 1572 655 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2291 2818 3044 3196 5305 1493 Size: 798 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 527 226 152 AseI AT'TA_AT Cuts at: 160 1032 2274 4063 160 Size: 872 1242 1789 1606 Fragments arranged by size: 1789 1606 1242 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2834 1870 Size: 73 114 777 4545 Fragments arranged by size: 4545 777 114 73 BamHI G'GATC_C Cuts at: 2204 2839 3206 2204 Size: 635 367 4507 Fragments arranged by size: 4507 635 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2830 4285 618 Size: 325 217 627 1043 1455 1842 Fragments arranged by size: 1842 1455 1043 627 325 217 BanII G_rGCy'C Cuts at: 1391 2446 2606 2828 1391 Size: 1055 160 222 4072 Fragments arranged by size: 4072 1055 222 160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4538 BbvCI CC'TCA_GC Cuts at: 2343 2534 2343 Size: 191 5318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2369 2403 3700 3734 1903 Size: 34 432 34 1297 34 3678 Fragments arranged by size: 3678 1297 432 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5504 5504 Size: 5509 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 3570 4726 1790 Size: 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2614 4169 1239 Size: 1203 172 1555 2579 Fragments arranged by size: 2579 1555 1203 172 Bpu10I CC'TnA_GC Cuts at: 2343 2534 2343 Size: 191 5318 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3634 4499 4797 3634 Size: 865 298 4346 Fragments arranged by size: 4346 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2402 3316 3698 4333 Size: 53 83 186 936 869 914 382 635 Cuts at: 4333 275 Size: 1451 Fragments arranged by size: 1451 936 914 869 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 4340 4614 4761 5172 3942 Size: 398 274 147 411 4279 Fragments arranged by size: 4279 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2160 2190 2377 2407 2440 Size: 30 165 30 757 30 187 30 33 Cuts at: 2440 2470 2612 2642 1178 Size: 30 142 30 4045 Fragments arranged by size: 4045 757 187 165 142 33 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2655 1186 Size: 1469 4040 BseYI C'CCAG_C Cuts at: 2656 4712 5208 2656 Size: 2056 496 2957 Fragments arranged by size: 2957 2056 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4936 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 2959 3720 4748 1540 Size: 275 186 80 878 761 1028 2301 Fragments arranged by size: 2301 1028 878 761 275 186 80 BsiHKAI G_wGCw'C Cuts at: 2446 2606 3570 3655 4726 2446 Size: 160 964 85 1071 3229 Fragments arranged by size: 3229 1071 964 160 85 BsmI GAATG_Cn' Cuts at: 2319 3020 3113 2319 Size: 701 93 4715 Fragments arranged by size: 4715 701 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2446 2606 2828 3570 3655 4726 1391 Size: 399 656 160 222 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 656 399 222 160 85 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 2278 2751 3398 5128 2278 Size: 473 647 1730 2659 Fragments arranged by size: 2659 1730 647 473 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3396 1394 Size: 429 1573 3507 Fragments arranged by size: 3507 1573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2044 4004 4178 65 Size: 1183 796 1960 174 1396 Fragments arranged by size: 1960 1396 1183 796 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2727 4153 4340 1700 Size: 110 917 1426 187 2869 Fragments arranged by size: 2869 1426 917 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5509 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2204 2839 3206 3591 3608 5060 5146 5158 Size: 232 635 367 385 17 1452 86 12 Cuts at: 5158 5504 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 635 385 367 346 232 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2051 2051 Size: 5509 BtgI C'CryG_G Cuts at: 513 1065 2081 513 Size: 552 1016 3941 Fragments arranged by size: 3941 1016 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4770 Fragments arranged by size: 4770 683 56 BtsI GCAGTG_nn' Cuts at: 2581 2641 2800 3025 3819 3839 2581 Size: 60 159 225 794 20 4251 Fragments arranged by size: 4251 794 225 159 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2116 2813 2116 Size: 697 4812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2729 2956 Size: 54 1425 50 275 18 248 651 227 Cuts at: 2956 3845 5474 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 651 275 248 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2078 2956 1537 Size: 275 266 878 4090 Fragments arranged by size: 4090 878 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 2593 2611 3439 1353 Size: 1240 18 828 3423 Fragments arranged by size: 3423 1240 828 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 4470 4616 387 Size: 1064 138 2507 374 146 1280 Fragments arranged by size: 2507 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2444 2604 2444 Size: 160 5349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2442 2562 2574 2614 3572 4169 4956 Size: 393 810 120 12 40 958 597 787 Cuts at: 4956 5224 1239 Size: 268 1524 Fragments arranged by size: 1524 958 810 787 597 393 268 120 40 12 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2168 2168 Size: 5509 FspI TGC'GCA Cuts at: 4015 4015 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 4656 1678 Size: 2978 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2160 2192 2480 2512 2612 Size: 32 135 32 588 32 288 32 100 Cuts at: 2612 2644 4150 4182 4224 4256 1373 Size: 32 1506 32 42 32 2626 Fragments arranged by size: 2626 1506 588 288 135 100 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2548 2853 3099 669 Size: 988 281 610 305 246 3079 Fragments arranged by size: 3079 988 610 305 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2443 2471 2493 4623 4807 1305 Size: 1138 28 22 2130 184 2007 Fragments arranged by size: 2130 2007 1138 184 28 22 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3687 Fragments arranged by size: 3687 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2324 3467 3826 3985 518 Size: 648 1056 102 1143 359 159 2042 Fragments arranged by size: 2042 1143 1056 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2026 2083 2925 3602 4750 4995 1606 Size: 420 57 842 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 420 245 57 NaeI GCC'GGC Cuts at: 2729 2729 Size: 5509 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4957 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NgoMIV G'CCGG_C Cuts at: 2727 2727 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2078 2956 2078 Size: 878 4631 NspI r_CATG'y Cuts at: 1132 1229 2867 4412 1132 Size: 97 1638 1545 2229 Fragments arranged by size: 2229 1638 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PstI C_TGCA'G Cuts at: 830 2861 830 Size: 2031 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 SacI G_AGCT'C Cuts at: 2446 2606 2446 Size: 160 5349 SacII CC_GC'GG Cuts at: 2084 2084 Size: 5509 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SapI GCTCTTCn'nnn_ Cuts at: 2593 2611 2593 Size: 18 5491 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2127 2857 3992 4673 4864 826 Size: 219 1082 730 1135 681 191 1471 Fragments arranged by size: 1471 1135 1082 730 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2836 1872 Size: 964 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3613 4514 4776 820 Size: 197 926 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 2704 1517 Size: 1187 4322 StyI C'CwwG_G Cuts at: 513 1065 2948 513 Size: 552 1883 3074 Fragments arranged by size: 3074 1883 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 3536 3553 3706 3891 4336 325 Size: 1255 650 1306 17 153 185 445 1498 Fragments arranged by size: 1498 1306 1255 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 5469 5501 2377 Size: 32 3060 32 2385 Fragments arranged by size: 3060 2385 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2834 1870 Size: 964 4545 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2403 3317 276 Size: 53 83 186 936 869 914 2468 Fragments arranged by size: 2468 936 914 869 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI