################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI AlwI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbsI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI MamI MfeI MflI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 866 TCCGGA T^CCGGA AflIII 772 ACGTGT A^C[GA][CT]GT Alw44I 1418 GTGCAC G^TGCAC AocI 102 CCTGAGG CC^T[ACGT]AGG ApaLI 1418 GTGCAC G^TGCAC Asp700I 1578 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 837 GGACC G^G[AT]CC BalI 746 TGGCCA TGG^CCA BfaI 1000 CTAG C^TAG BsaI 908 GAGACC GGTCTC(1/5) BsaWI 866 TCCGGA [AT]^CCGG[AT] BseAI 866 TCCGGA T^CCGGA BspEI 866 TCCGGA T^CCGGA BsrDI 1202 GCAATG GCAATG(2/0) BstAPI 1716 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 542 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 102 CCTGAGG CC^T[ACGT]AGG DpnII 351 GATC ^GATC DraIII 354 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1156 CCGCGG C^C[GA][CT]GG Eco81I 102 CCTGAGG CC^T[ACGT]AGG EcoRV 1020 GATATC GAT^ATC HincII 1054 GTCAAC GT[CT]^[GA]AC HindII 1054 GTCAAC GT[CT]^[GA]AC KspI 1156 CCGCGG CCGC^GG MaeI 1000 CTAG C^TAG MaeIII 623 GTGAC ^GT[ACGT]AC MboI 351 GATC ^GATC MluNI 746 TGGCCA TGG^CCA MroI 866 TCCGGA T^CCGGA MscI 746 TGGCCA TGG^CCA NdeII 351 GATC ^GATC NspI 791 GCATGT [GA]CATG^[CT] PaeR7I 1709 CTCGAG C^TCGAG PflMI 738 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 373 GACTC GAGTC(4/5) SacI 1040 GAGCTC GAGCT^C SacII 1156 CCGCGG CCGC^GG Sau3AI 351 GATC ^GATC SfiI 1213 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1709 CTCGAG C^T[CT][GA]AG StuI 1088 AGGCCT AGG^CCT TfiI 136 GATTC G^A[AT]TC Tsp45I 623 GTGAC ^GT[GC]AC Van91I 738 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1709 CTCGAG C^TCGAG XmnI 1578 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 1515 GACGCC G[GA]^CG[CT]C 1660 GACGCC AlwNI 841 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 844 CAGCTGCTG BanI 516 GGTGCC G^G[CT][GA]CC 574 GGTGCC BbrPI 79 CACGTG CAC^GTG 771 CACGTG BbvCI 1131 CCTCAGC CCTCAGC(-5/-2) 1384 GCTGAGG BcgI 1581 CGACTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1725 GCAGGAATATCG BlpI 667 GCTGAGC GC^T[ACGT]AGC 1042 GCTCAGC Bpu1102I 667 GCTGAGC GC^T[ACGT]AGC 1042 GCTCAGC BsaAI 79 CACGTG [CT]AC^GT[GA] 771 CACGTG BsaHI 1515 GACGCC G[GA]^CG[CT]C 1660 GACGCC BsiEI 252 CGGCCG CG[GA][CT]^CG 1221 CGGCCG BsmBI 418 GAGACG CGTCTC(1/5) 1513 GAGACG BsmFI 836 GGGAC GGGAC(10/14) 606 GTCCC Bsp1407I 775 TGTACA T^GTACA 794 TGTACA BsrGI 775 TGTACA T^GTACA 794 TGTACA CelII 667 GCTGAGC GC^T[ACGT]AGC 1042 GCTCAGC EagI 252 CGGCCG C^GGCCG 1221 CGGCCG EarI 1551 CTCTTC CTCTTC(1/4) 567 GAAGAG EclXI 252 CGGCCG C^GGCCG 1221 CGGCCG Eco52I 252 CGGCCG C^GGCCG 1221 CGGCCG EcoNI 699 CCTGACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 735 CCTCCAGCAGG EcoT14I 177 CCAAGG C^C[AT][AT]GG 903 CCTTGG Hin1I 1515 GACGCC G[GA]^CG[CT]C 1660 GACGCC HinfI 136 GATTC G^A[ACGT]TC 373 GACTC Ksp632I 1551 CTCTTC CTCTTC(1/4) 567 GAAGAG PmaCI 79 CACGTG CAC^GTG 771 CACGTG PmlI 79 CACGTG CAC^GTG 771 CACGTG PvuII 844 CAGCTG CAG^CTG 1846 CAGCTG SfaNI 284 GCATC GCATC(5/9) 1634 GCATC SmaI 1104 CCCGGG CCC^GGG 1397 CCCGGG SspBI 775 TGTACA T^GTACA 794 TGTACA StyI 177 CCAAGG C^C[AT][AT]GG 903 CCTTGG XmaCI 1104 CCCGGG C^CCGGG 1397 CCCGGG XmaI 1104 CCCGGG C^CCGGG 1397 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AfaI AluI ApaI AspHI AvaI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfcI TaqI TseI TspRI TthHB8I