(Circular) (Six-base) MAPSORT of: pFN21AB3035.seq Check: 9296 from: 1 to: 6568 pFN21AB3035 6568 bp With 182 enzymes: SgfI * April 28, 2014 11:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2737 3162 3436 2737 Size: 425 274 5869 Fragments arranged by size: 5869 425 274 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 1937 Size: 1974 4594 Acc65I G'GTAC_C Cuts at: 3889 3889 Size: 6568 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2876 3557 4631 6015 6283 1632 Size: 404 840 681 1074 1384 268 1917 Fragments arranged by size: 1917 1384 1074 840 681 404 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6371 AflIII A'CryG_T Cuts at: 1203 2777 5467 6444 1203 Size: 1574 2690 977 1327 Fragments arranged by size: 2690 1574 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3170 1184 Size: 1986 4582 AlwNI CAG_nnn'CTG Cuts at: 2851 2854 4311 5883 2851 Size: 3 1457 1572 3536 Fragments arranged by size: 3536 1572 1457 3 ApaI G_GGCC'C Cuts at: 3116 3184 3409 3116 Size: 68 225 6275 Fragments arranged by size: 6275 225 68 ApaLI G'TGCA_C Cuts at: 3423 4625 5781 3423 Size: 1202 1156 4210 Fragments arranged by size: 4210 1202 1156 ApoI r'AATT_y Cuts at: 1493 3877 4103 4255 6364 1493 Size: 2384 226 152 2109 1697 Fragments arranged by size: 2384 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 3109 3175 3402 3714 3893 1870 Size: 73 1166 66 227 312 179 4545 Fragments arranged by size: 4545 1166 312 227 179 73 66 BamHI G'GATC_C Cuts at: 3898 4265 3898 Size: 367 6201 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2521 2579 3889 5344 618 Size: 325 217 627 734 58 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 734 627 325 217 58 BanII G_rGCy'C Cuts at: 1391 3049 3116 3174 3184 3409 3454 3695 3887 Size: 1658 67 58 10 225 45 241 192 Cuts at: 3887 1391 Size: 4072 Fragments arranged by size: 4072 1658 241 225 192 67 58 45 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5597 BbvCI CC'TCA_GC Cuts at: 3137 3390 3137 Size: 253 6315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3575 3609 3719 3753 4759 4793 1903 Size: 34 1638 34 110 34 1006 34 3678 Fragments arranged by size: 3678 1638 1006 110 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4460 5670 4460 Size: 1210 5358 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2111 2937 Size: 107 122 71 631 605 337 102 826 Cuts at: 2937 3163 3225 3316 5179 136 Size: 226 62 91 1863 1525 Fragments arranged by size: 1863 1525 826 631 605 337 226 122 107 102 91 71 62 BglII A'GATC_T Cuts at: 6563 6563 Size: 6568 BlpI GC'TnA_GC Cuts at: 2673 3048 3985 2673 Size: 375 937 5256 Fragments arranged by size: 5256 937 375 Bme1580I G_kGCm'C Cuts at: 1790 2107 2190 2526 3081 3116 3184 3307 3409 Size: 317 83 336 555 35 68 123 102 Cuts at: 3409 3427 4629 5785 1790 Size: 18 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 555 336 317 123 102 83 68 35 18 BmgBI CAC'GTC Cuts at: 1318 3507 3513 1318 Size: 2189 6 4373 Fragments arranged by size: 4373 2189 6 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4299 5257 442 Size: 416 53 83 399 24 2882 958 1753 Fragments arranged by size: 2882 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6568 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2290 2478 2726 2911 3127 3188 5228 1239 Size: 1051 188 248 185 216 61 2040 2579 Fragments arranged by size: 2579 2040 1051 248 216 188 185 61 Bpu10I CC'TnA_GC Cuts at: 2766 3137 3390 2766 Size: 371 253 5944 Fragments arranged by size: 5944 371 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4693 5558 5856 4693 Size: 865 298 5405 Fragments arranged by size: 5405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2907 5231 882 Size: 617 1408 2324 2219 Fragments arranged by size: 2324 2219 1408 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 2778 4359 493 Size: 711 882 692 1581 2702 Fragments arranged by size: 2702 1581 882 711 692 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3521 3666 4375 4757 Size: 53 83 186 936 1988 145 709 382 Cuts at: 4757 5392 275 Size: 635 1451 Fragments arranged by size: 1988 1451 936 709 635 382 186 145 83 53 BsaWI w'CCGG_w Cuts at: 2871 5001 5399 5673 5820 6231 2871 Size: 2130 398 274 147 411 3208 Fragments arranged by size: 3208 2130 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2422 2452 1178 Size: 30 165 30 1019 30 5294 Fragments arranged by size: 5294 1019 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 2435 2729 3544 3713 1186 Size: 1246 3 294 815 169 4041 Fragments arranged by size: 4041 1246 815 294 169 3 BseYI C'CCAG_C Cuts at: 2202 3070 3259 3306 3311 3408 3454 3657 3669 Size: 868 189 47 5 97 46 203 12 Cuts at: 3669 5771 6267 2202 Size: 2102 496 2503 Fragments arranged by size: 2503 2102 868 496 203 189 97 47 46 12 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 2831 3605 3782 1163 Size: 573 888 207 774 177 3949 Fragments arranged by size: 3949 888 774 573 207 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2260 3229 4018 4779 5807 1540 Size: 275 186 259 969 789 761 1028 2301 Fragments arranged by size: 2301 1028 969 789 761 275 259 186 BsiHKAI G_wGCw'C Cuts at: 2868 2897 3049 3243 3427 4629 4714 5785 2868 Size: 29 152 194 184 1202 85 1071 3651 Fragments arranged by size: 3651 1202 1071 194 184 152 85 29 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 3512 2417 Size: 1095 5473 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2190 2526 2868 2897 3049 3081 Size: 399 317 83 336 342 29 152 32 Cuts at: 3081 3116 3174 3184 3243 3307 3409 3427 3454 Size: 35 58 10 59 64 102 18 27 Cuts at: 3454 3695 3887 4629 4714 5785 1391 Size: 241 192 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 336 317 241 192 152 102 85 83 64 59 58 35 32 29 27 18 10 BspEI T'CCGG_A Cuts at: 2871 6231 2871 Size: 3360 3208 BspHI T'CATG_A Cuts at: 4457 6187 4457 Size: 1730 4838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2737 3162 3436 3491 3627 3804 3923 844 Size: 1893 425 274 55 136 177 119 3489 Fragments arranged by size: 3489 1893 425 274 177 136 119 55 BsrBI CCG'CTC Cuts at: 1394 1823 4455 1394 Size: 429 2632 3507 Fragments arranged by size: 3507 2632 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3214 5063 5237 65 Size: 1183 1966 1849 174 1396 Fragments arranged by size: 1966 1849 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5212 5399 1700 Size: 110 3402 187 2869 Fragments arranged by size: 3402 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2780 2799 96 Size: 2684 19 3865 Fragments arranged by size: 3865 2684 19 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 3727 794 Size: 2933 3635 BstBI TT'CG_AA Cuts at: 5372 5372 Size: 6568 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstXI CCAn_nnnn'nTGG Cuts at: 2554 2554 Size: 6568 BstYI r'GATC_y Cuts at: 1972 3898 4265 4650 4667 6119 6205 6217 6563 Size: 1926 367 385 17 1452 86 12 346 Cuts at: 6563 1972 Size: 1977 Fragments arranged by size: 1977 1926 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2108 2108 Size: 6568 BtgI C'CryG_G Cuts at: 513 1065 2004 3161 513 Size: 552 939 1157 3920 Fragments arranged by size: 3920 1157 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3814 487 Size: 683 56 2588 3241 Fragments arranged by size: 3241 2588 683 56 BtsI GCAGTG_nn' Cuts at: 2783 4084 4878 4898 2783 Size: 1301 794 20 4453 Fragments arranged by size: 4453 1301 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DraIII CAC_nnn'GTG Cuts at: 2364 2364 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2257 2751 3085 Size: 54 1425 50 275 18 427 494 334 Cuts at: 3085 3195 3226 4015 4904 6533 8 Size: 110 31 789 889 1629 43 Fragments arranged by size: 1629 1425 889 789 494 427 334 275 110 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2257 3226 4015 1537 Size: 275 445 969 789 4090 Fragments arranged by size: 4090 969 789 445 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2567 3562 4498 1353 Size: 1214 995 936 3423 Fragments arranged by size: 3423 1214 995 936 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2429 2729 5155 5529 5675 387 Size: 1064 138 840 300 2426 374 146 1280 Fragments arranged by size: 2426 1280 1064 840 374 300 146 138 EcoICRI GAG'CTC Cuts at: 3047 3047 Size: 6568 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2290 2478 2726 2876 2911 3127 Size: 393 404 254 188 248 150 35 216 Cuts at: 3127 3188 3557 4631 5228 6015 6283 1239 Size: 61 369 1074 597 787 268 1524 Fragments arranged by size: 1524 1074 787 597 404 393 369 268 254 248 216 188 150 61 35 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2744 2708 Size: 36 6532 EcoO109I rG'GnC_Cy Cuts at: 2112 2619 3180 2112 Size: 507 561 5500 Fragments arranged by size: 5500 561 507 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 EcoRV GAT'ATC Cuts at: 3027 3027 Size: 6568 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3604 3604 Size: 6568 FspI TGC'GCA Cuts at: 5074 5074 Size: 6568 HaeII r_GCGC'y Cuts at: 1678 2015 2953 3571 5715 1678 Size: 337 938 618 2144 2531 Fragments arranged by size: 2531 2144 938 618 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5209 5241 5283 5315 1373 Size: 32 135 32 3637 32 42 32 2626 Fragments arranged by size: 3637 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3061 3912 4158 669 Size: 988 281 1123 851 246 3079 Fragments arranged by size: 3079 1123 988 851 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6568 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KpnI G_GTAC'C Cuts at: 3893 3893 Size: 6568 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 3495 5682 5866 1305 Size: 969 1221 2187 184 2007 Fragments arranged by size: 2187 2007 1221 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2753 10 Size: 54 1425 343 921 3825 Fragments arranged by size: 3825 1425 921 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4526 4885 5044 518 Size: 648 3360 359 159 2042 Fragments arranged by size: 3360 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2604 2851 3163 3225 3786 3847 3853 3984 Size: 998 247 312 62 561 61 6 131 Cuts at: 3984 4661 5809 6054 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 561 312 247 245 131 62 61 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5077 Fragments arranged by size: 5077 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6568 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 2800 3926 5471 1132 Size: 97 1571 1126 1545 2229 Fragments arranged by size: 2229 1571 1545 1126 97 PasI CC'CwG_GG Cuts at: 1348 2272 3688 1348 Size: 924 1416 4228 Fragments arranged by size: 4228 1416 924 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2749 1108 Size: 1641 4927 PfoI T'CCnGG_A Cuts at: 2528 2528 Size: 6568 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PmlI CAC'GTG Cuts at: 2086 2778 2086 Size: 692 5876 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 6150 6182 4637 Size: 32 1481 32 5023 Fragments arranged by size: 5023 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PspOMI G'GGCC_C Cuts at: 3112 3180 3405 3112 Size: 68 225 6275 Fragments arranged by size: 6275 225 68 PspXI vC'TCGA_Gb Cuts at: 3714 3714 Size: 6568 PstI C_TGCA'G Cuts at: 830 2694 2790 2982 3488 3858 3920 830 Size: 1864 96 192 506 370 62 3478 Fragments arranged by size: 3478 1864 506 370 192 96 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2851 3853 2851 Size: 1002 5566 SacI G_AGCT'C Cuts at: 3049 3049 Size: 6568 SacII CC_GC'GG Cuts at: 3164 3164 Size: 6568 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SfcI C'TryA_G Cuts at: 826 1045 2690 2786 2978 3484 3766 3854 3916 Size: 219 1645 96 192 506 282 88 62 Cuts at: 3916 5051 5732 5923 826 Size: 1135 681 191 1471 Fragments arranged by size: 1645 1471 1135 681 506 282 219 192 191 96 88 62 SfiI GGCCn_nnn'nGGCC Cuts at: 3225 3225 Size: 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 3111 3404 3895 1872 Size: 1239 293 491 4545 Fragments arranged by size: 4545 1239 491 293 SmlI C'TyrA_G Cuts at: 820 1017 1943 3714 4672 5573 5835 820 Size: 197 926 1771 958 901 262 1553 Fragments arranged by size: 1771 1553 958 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4359 493 Size: 3866 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 3095 1517 Size: 1578 4990 StyI C'CwwG_G Cuts at: 513 1065 2004 2182 2908 4007 513 Size: 552 939 178 726 1099 3074 Fragments arranged by size: 3074 1099 939 726 552 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2646 3449 3671 4595 4612 4765 4950 Size: 1255 1066 803 222 924 17 153 185 Cuts at: 4950 5395 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1066 924 803 445 222 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2780 2799 3600 Size: 274 80 33 51 1443 803 19 801 Cuts at: 3600 4814 6526 96 Size: 1214 1712 138 Fragments arranged by size: 1712 1443 1214 803 801 274 138 80 51 33 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2422 2454 2563 2595 2762 2794 6528 6560 2422 Size: 32 109 32 167 32 3734 32 2430 Fragments arranged by size: 3734 2430 167 109 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6568 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 3714 1943 Size: 1771 4797 XmaI C'CCGG_G Cuts at: 1870 3109 3402 3893 1870 Size: 1239 293 491 4545 Fragments arranged by size: 4545 1239 491 293 XmnI GAAnn'nnTTC Cuts at: 3587 4697 3587 Size: 1110 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI XcmI