Restriction map for
pFN21AE2368
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# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AscI AseI AsnI
AvrII BbrPI BclI BfrI BlnI
BsiWI BsmI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EcoRV
EcoT22I FbaI FseI HindIII HpaI
MfeI MunI NotI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 4220 GGTACC G^GTACC
AccI 3832 GTAGAC GT^[AC][GT]AC
AgeI 1016 ACCGGT A^CCGGT
AhdI 6028 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4220 GGTACC G^GTACC
AspEI 6028 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5929 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3982 GGCGCC GGCGC^C
BciVI 1359 GGATAC GTATCC(6/5)
BsaAI 5845 TACGTG [CT]AC^GT[GA]
BsaBI 2109 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5126 CGGCCG CG[GA][CT]^CG
BssSI 1333 CACGAG CACGAG(-5/-1)
BstEII 4623 GGTGACC G^GT[ACGT]ACC
BstPI 4623 GGTGACC G^GT[ACGT]ACC
DraI 4249 TTTAAA TTT^AAA
EagI 5126 CGGCCG C^GGCCG
Eam1105I 6028 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5126 CGGCCG C^GGCCG
Eco52I 5126 CGGCCG C^GGCCG
EcoO65I 4623 GGTGACC G^GT[ACGT]ACC
EcoRI 2230 GAATTC G^AATTC
KasI 3982 GGCGCC G^GCGCC
KpnI 4220 GGTACC GGTAC^C
MamI 2109 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 551 ACGCGT A^CGCGT
MseI 4250 TTAA T^TAA
NaeI 5128 GCCGGC GCC^GGC
NarI 3982 GGCGCC GG^CGCC
NdeI 3737 CATATG CA^TATG
NgoMIV 5128 GCCGGC G^CCGGC
NheI 2535 GCTAGC G^CTAGC
PaeR7I 533 CTCGAG C^TCGAG
PflFI 5929 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1016 ACCGGT A^CCGGT
PshAI 4648 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 171 CCTGCAGG CCTGCA^GG
SfoI 3982 GGCGCC GGC^GCC
SmaI 3115 CCCGGG CCC^GGG
Sse8387I 171 CCTGCAGG CCTGCA^GG
Tru9I 4250 TTAA T^TAA
Tth111I 5929 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 533 CTCGAG C^TCGAG
XmaCI 3115 CCCGGG C^CCGGG
XmaI 3115 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 3835 GACGTC GACGT^C
4588 GACGTC
Alw44I 414 GTGCAC G^TGCAC
3914 GTGCAC
AocI 825 CCTAAGG CC^T[ACGT]AGG
1143 CCTCAGG
ApaLI 414 GTGCAC G^TGCAC
3914 GTGCAC
AviII 842 TGCGCA TGC^GCA
3578 TGCGCA
BamHI 3120 GGATCC G^GATCC
4922 GGATCC
BglI 1908 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2628 GCCTCGCTGGC
BlpI 2814 GCTCAGC GC^T[ACGT]AGC
3439 GCTGAGC
Bpu1102I 2814 GCTCAGC GC^T[ACGT]AGC
3439 GCTGAGC
BsgI 547 CTGCAC GTGCAG(16/14)
2828 GTGCAG
Bsp1407I 4334 TGTACA T^GTACA
5294 TGTACA
BsrBI 365 GAGCGG CCGCTC(-3/-3)
3688 GAGCGG
BsrFI 1016 ACCGGT [GA]^CCGG[CT]
5128 GCCGGC
BsrGI 4334 TGTACA T^GTACA
5294 TGTACA
Bsu36I 825 CCTAAGG CC^T[ACGT]AGG
1143 CCTCAGG
CelII 2814 GCTCAGC GC^T[ACGT]AGC
3439 GCTGAGC
Cfr10I 1016 ACCGGT [GA]^CCGG[CT]
5128 GCCGGC
DraIII 2169 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
3595 CACATCGTG
Eco81I 825 CCTAAGG CC^T[ACGT]AGG
1143 CCTCAGG
FspI 842 TGCGCA TGC^GCA
3578 TGCGCA
HincII 4195 GTCAAC GT[CT]^[GA]AC
5287 GTCAAC
HindII 4195 GTCAAC GT[CT]^[GA]AC
5287 GTCAAC
KspI 99 CCGCGG CCGC^GG
1575 CCGCGG
SacII 99 CCGCGG CCGC^GG
1575 CCGCGG
SexAI 1208 ACCTGGT A^CC[AT]GGT
2258 ACCTGGT
SspBI 4334 TGTACA T^GTACA
5294 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI