(Circular) (Six-base) MAPSORT of: pFN21AE2368.seq Check: 6275 from: 1 to: 10750 pFN21AE2368 10750 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6232 7755 6232 Size: 1523 9227 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5844 6597 8560 278 Size: 53 83 186 936 4308 753 1963 2468 Fragments arranged by size: 4308 2468 1963 936 753 186 83 53 AccI GT'mk_AC Cuts at: 1937 5838 8093 1937 Size: 3901 2255 4594 Fragments arranged by size: 4594 3901 2255 Acc65I G'GTAC_C Cuts at: 6225 8071 6225 Size: 1846 8904 AclI AA'CG_TT Cuts at: 1563 8877 9250 1563 Size: 7314 373 3063 Fragments arranged by size: 7314 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 3438 3642 4813 5529 6028 7309 7634 Size: 1100 706 204 1171 716 499 1281 325 Cuts at: 7634 8813 10197 10465 1632 Size: 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1281 1179 1171 1100 716 706 499 325 268 204 AfeI AGC'GCT Cuts at: 2862 3302 4082 5664 2862 Size: 440 780 1582 7948 Fragments arranged by size: 7948 1582 780 440 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10553 AflIII A'CryG_T Cuts at: 1203 2556 3005 6336 7071 7296 9649 10626 1203 Size: 1353 449 3331 735 225 2353 977 1327 Fragments arranged by size: 3331 2353 1353 1327 977 735 449 225 AgeI A'CCGG_T Cuts at: 3021 9581 3021 Size: 6560 4190 AhdI GACnn_n'nnGTC Cuts at: 8038 9479 8038 Size: 1441 9309 AleI CACnn'nnGTG Cuts at: 1166 6131 1166 Size: 4965 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7763 1184 Size: 6579 4171 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3733 3765 7619 7651 3733 Size: 32 3854 32 6832 Fragments arranged by size: 6832 3854 32 32 AlwNI CAG_nnn'CTG Cuts at: 3461 3931 4853 5283 5303 8493 10065 3461 Size: 470 922 430 20 3190 1572 4146 Fragments arranged by size: 4146 3190 1572 922 470 430 20 ApaI G_GGCC'C Cuts at: 2683 2731 3924 3991 5894 2683 Size: 48 1193 67 1903 7539 Fragments arranged by size: 7539 1903 1193 67 48 ApaLI G'TGCA_C Cuts at: 2419 5919 8807 9963 2419 Size: 3500 2888 1156 3206 Fragments arranged by size: 3500 3206 2888 1156 ApoI r'AATT_y Cuts at: 1493 2627 4235 7400 8059 8285 8437 10546 1493 Size: 1134 1608 3165 659 226 152 2109 1697 Fragments arranged by size: 3165 2109 1697 1608 1134 659 226 152 AseI AT'TA_AT Cuts at: 160 1032 9304 160 Size: 872 8272 1606 Fragments arranged by size: 8272 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10750 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2144 2538 5120 7215 8075 1870 Size: 73 82 119 394 2582 2095 860 4545 Fragments arranged by size: 4545 2582 2095 860 394 119 82 73 BamHI G'GATC_C Cuts at: 5125 6927 8080 8447 5125 Size: 1802 1153 367 7428 Fragments arranged by size: 7428 1802 1153 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2870 2960 3024 3691 Size: 325 217 627 243 840 90 64 667 Cuts at: 3691 5059 5114 5987 6225 7743 8071 9526 618 Size: 1368 55 873 238 1518 328 1455 1842 Fragments arranged by size: 1842 1518 1455 1368 873 840 667 627 328 325 243 238 217 90 64 55 BanII G_rGCy'C Cuts at: 1391 2229 2241 2683 2731 3924 3991 5136 5451 Size: 838 12 442 48 1193 67 1145 315 Cuts at: 5451 5894 5941 6509 7235 7356 7674 8069 1391 Size: 443 47 568 726 121 318 395 4072 Fragments arranged by size: 4072 1193 1145 838 726 568 443 442 395 318 315 121 67 48 47 12 BbeI G_GCGC'C Cuts at: 5991 5991 Size: 10750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2720 2770 3444 4261 4441 4750 5705 Size: 971 821 50 674 817 180 309 955 Cuts at: 5705 5740 928 Size: 35 5938 Fragments arranged by size: 5938 971 955 821 817 674 309 180 50 35 BbvCI CC'TCA_GC Cuts at: 2786 3548 3992 4607 7151 2786 Size: 762 444 615 2544 6385 Fragments arranged by size: 6385 2544 762 615 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2529 2563 3059 3093 5309 5343 7802 Size: 34 592 34 496 34 2216 34 2459 Cuts at: 7802 7836 8941 8975 1903 Size: 34 1105 34 3678 Fragments arranged by size: 3678 2459 2216 1105 592 496 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3358 8642 9852 3358 Size: 5284 1210 4256 Fragments arranged by size: 5284 4256 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10750 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3919 4639 9361 Size: 107 122 71 631 605 2247 720 4722 Cuts at: 9361 136 Size: 1525 Fragments arranged by size: 4722 2247 1525 720 631 605 122 107 71 BglII A'GATC_T Cuts at: 2200 3165 7389 7659 7999 10745 2200 Size: 965 4224 270 340 2746 2205 Fragments arranged by size: 4224 2746 2205 965 340 270 BlpI GC'TnA_GC Cuts at: 4820 5445 8167 4820 Size: 625 2722 7403 Fragments arranged by size: 7403 2722 625 Bme1580I G_kGCm'C Cuts at: 1790 2033 2423 2683 2731 3696 3721 3924 3991 Size: 243 390 260 48 965 25 203 67 Cuts at: 3991 4519 5117 5148 5210 5894 5923 6818 8811 Size: 528 598 31 62 684 29 895 1993 Cuts at: 8811 9967 1790 Size: 1156 2573 Fragments arranged by size: 2573 1993 1156 965 895 684 598 528 390 260 243 203 67 62 48 31 29 25 BmgBI CAC'GTC Cuts at: 1318 7128 1318 Size: 5810 4940 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3401 3726 3824 Size: 416 53 83 399 24 1984 325 98 Cuts at: 3824 4529 7179 8481 9439 442 Size: 705 2650 1302 958 1753 Fragments arranged by size: 2650 1984 1753 1302 958 705 416 399 325 98 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4544 1056 Size: 3488 7262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7856 7885 7856 Size: 29 10721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 4232 4865 5305 5474 5777 6277 7337 Size: 1025 1968 633 440 169 303 500 1060 Cuts at: 7337 7876 7949 9410 1239 Size: 539 73 1461 2579 Fragments arranged by size: 2579 1968 1461 1060 1025 633 539 500 440 303 169 73 Bpu10I CC'TnA_GC Cuts at: 2786 3548 3992 4376 4607 7151 7827 2786 Size: 762 444 384 231 2544 676 5709 Fragments arranged by size: 5709 2544 762 676 444 384 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2936 3311 5738 5753 8875 9740 10038 2936 Size: 375 2427 15 3122 865 298 3648 Fragments arranged by size: 3648 3122 2427 865 375 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3275 3603 3811 4700 4958 5384 9413 Size: 617 1776 328 208 889 258 426 4029 Cuts at: 9413 882 Size: 2219 Fragments arranged by size: 4029 2219 1776 889 617 426 328 258 208 BsaAI yAC'GTr Cuts at: 493 1204 7852 8541 493 Size: 711 6648 689 2702 Fragments arranged by size: 6648 2702 711 689 BsaBI GATnn'nnATC Cuts at: 4118 10744 4118 Size: 6626 4124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5841 5988 6594 7608 Size: 53 83 186 936 4308 147 606 1014 Cuts at: 7608 8557 8939 9574 275 Size: 949 382 635 1451 Fragments arranged by size: 4308 1451 1014 949 936 635 606 382 186 147 83 53 BsaWI w'CCGG_w Cuts at: 2313 2364 3010 3021 5249 5269 6170 8023 9183 Size: 51 646 11 2228 20 901 1853 1160 Cuts at: 9183 9581 9855 10002 10413 2313 Size: 398 274 147 411 2650 Fragments arranged by size: 2650 2228 1853 1160 901 646 411 398 274 147 51 20 11 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3733 3763 5204 5234 5393 Size: 30 165 30 2330 30 1441 30 159 Cuts at: 5393 5423 6836 6866 7619 7649 1178 Size: 30 1413 30 753 30 4279 Fragments arranged by size: 4279 2330 1441 1413 753 165 159 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2165 3269 3302 3451 3479 3848 3923 Size: 976 3 1104 33 149 28 369 75 Cuts at: 3923 3980 4049 4256 5207 5228 5231 5299 5705 Size: 57 69 207 951 21 3 68 406 Cuts at: 5705 6005 6482 7139 7355 7643 7888 7897 1186 Size: 300 477 657 216 288 245 9 4039 Fragments arranged by size: 4039 1104 976 951 657 477 406 369 300 288 245 216 207 149 75 69 68 57 33 28 21 9 3 3 BseYI C'CCAG_C Cuts at: 2166 2451 2999 3123 4077 4390 5009 5238 6502 Size: 285 548 124 954 313 619 229 1264 Cuts at: 6502 7137 7234 9953 10449 2166 Size: 635 97 2719 496 2467 Fragments arranged by size: 2719 2467 1264 954 635 619 548 496 313 285 229 124 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 4854 1163 Size: 573 801 2317 7059 Fragments arranged by size: 7059 2317 801 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7134 8200 8961 9989 1540 Size: 275 186 5133 1066 761 1028 2301 Fragments arranged by size: 5133 2301 1066 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2423 3882 3979 4218 5451 5613 5923 5941 Size: 194 1459 97 239 1233 162 310 18 Cuts at: 5941 6825 7349 8811 8896 9967 2229 Size: 884 524 1462 85 1071 3012 Fragments arranged by size: 3012 1462 1459 1233 1071 884 524 310 239 194 162 97 85 18 BsmI GAATG_Cn' Cuts at: 8261 8354 8261 Size: 93 10657 BsmBI CGTCTCn'nnnn_ Cuts at: 4076 5042 5848 4076 Size: 966 806 8978 Fragments arranged by size: 8978 966 806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2229 2241 2423 2683 2731 3696 Size: 399 243 196 12 182 260 48 965 Cuts at: 3696 3721 3882 3924 3979 3991 4218 4519 5117 Size: 25 161 42 55 12 227 301 598 Cuts at: 5117 5136 5148 5210 5451 5613 5894 5923 5941 Size: 19 12 62 241 162 281 29 18 Cuts at: 5941 6509 6818 6825 7235 7349 7356 7674 8069 Size: 568 309 7 410 114 7 318 395 Cuts at: 8069 8811 8896 9967 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 965 742 598 568 410 399 395 318 309 301 281 260 243 241 227 196 182 162 161 114 85 62 55 48 42 29 25 19 18 12 12 12 7 7 BspEI T'CCGG_A Cuts at: 2364 3010 5269 8023 10413 2364 Size: 646 2259 2754 2390 2701 Fragments arranged by size: 2754 2701 2390 2259 646 BspHI T'CATG_A Cuts at: 5640 6003 6459 6951 8639 10369 5640 Size: 363 456 492 1688 1730 6021 Fragments arranged by size: 6021 1730 1688 492 456 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 2184 2955 4243 5600 6232 6702 7527 Size: 1193 147 771 1288 1357 632 470 825 Cuts at: 7527 7755 8105 844 Size: 228 350 3489 Fragments arranged by size: 3489 1357 1288 1193 825 771 632 470 350 228 147 BsrBI CCG'CTC Cuts at: 1394 1823 2372 5695 8637 1394 Size: 429 549 3323 2942 3507 Fragments arranged by size: 3507 3323 2942 549 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2650 3249 3525 3802 4308 5016 7213 Size: 1183 1402 599 276 277 506 708 2197 Cuts at: 7213 7302 7546 9245 9419 65 Size: 89 244 1699 174 1396 Fragments arranged by size: 2197 1699 1402 1396 1183 708 599 506 277 276 244 174 89 BsrFI r'CCGG_y Cuts at: 1700 1810 3021 7133 9394 9581 1700 Size: 110 1211 4112 2261 187 2869 Fragments arranged by size: 4112 2869 2261 1211 187 110 BsrGI T'GTAC_A Cuts at: 96 6339 7299 96 Size: 6243 960 3547 Fragments arranged by size: 6243 3547 960 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10750 BssSI C'ACGA_G Cuts at: 3338 8810 9822 3338 Size: 5472 1012 4266 Fragments arranged by size: 5472 4266 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3632 4988 5592 7107 7548 7750 794 Size: 2838 1356 604 1515 441 202 3794 Fragments arranged by size: 3794 2838 1515 1356 604 441 202 BstBI TT'CG_AA Cuts at: 9554 9554 Size: 10750 BstEII G'GTnAC_C Cuts at: 1184 6628 1184 Size: 5444 5306 BstXI CCAn_nnnn'nTGG Cuts at: 2586 4021 4062 5155 5872 6640 7315 2586 Size: 1435 41 1093 717 768 675 6021 Fragments arranged by size: 6021 1435 1093 768 717 675 41 BstYI r'GATC_y Cuts at: 1972 2200 3165 5125 5460 6927 7389 7617 7659 Size: 228 965 1960 335 1467 462 228 42 Cuts at: 7659 7999 8080 8447 8832 8849 10301 10387 10399 Size: 340 81 367 385 17 1452 86 12 Cuts at: 10399 10745 1972 Size: 346 1977 Fragments arranged by size: 1977 1960 1467 1452 965 462 385 367 346 340 335 228 228 86 81 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2831 3149 2831 Size: 318 10432 BtgI C'CryG_G Cuts at: 513 1065 2004 2104 2579 2883 3052 3234 3315 Size: 552 939 100 475 304 169 182 81 Cuts at: 3315 3541 3580 3766 6533 7239 7335 7524 7716 Size: 226 39 186 2767 706 96 189 192 Cuts at: 7716 513 Size: 3547 Fragments arranged by size: 3547 2767 939 706 552 475 304 226 192 189 186 182 169 100 96 81 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3259 5134 7828 487 Size: 683 56 2033 1875 2694 3409 Fragments arranged by size: 3409 2694 2033 1875 683 56 BtsI GCAGTG_nn' Cuts at: 3683 5360 5662 5903 8266 9060 9080 3683 Size: 1677 302 241 2363 794 20 5353 Fragments arranged by size: 5353 2363 1677 794 302 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10750 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10715 DraI TTT'AAA Cuts at: 6256 8054 6256 Size: 1798 8952 DraIII CAC_nnn'GTG Cuts at: 4179 5605 4179 Size: 1426 9324 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2900 3151 3732 9757 809 Size: 507 1584 251 581 6025 1802 Fragments arranged by size: 6025 1802 1584 581 507 251 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3231 3754 4458 Size: 54 1425 50 275 18 1401 523 704 Cuts at: 4458 5157 5473 5862 6681 6705 7029 7131 7332 Size: 699 316 389 819 24 324 102 201 Cuts at: 7332 7713 8197 9086 10715 8 Size: 381 484 889 1629 43 Fragments arranged by size: 1629 1425 1401 889 819 704 699 523 484 389 381 324 316 275 201 102 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 7131 8197 1537 Size: 275 5319 1066 4090 Fragments arranged by size: 5319 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 2822 3104 4020 4205 4808 5178 6300 Size: 1052 417 282 916 185 603 370 1122 Cuts at: 6300 6869 6977 6998 7118 7256 7698 7787 7809 Size: 569 108 21 120 138 442 89 22 Cuts at: 7809 8680 1353 Size: 871 3423 Fragments arranged by size: 3423 1122 1052 916 871 603 569 442 417 370 282 185 138 120 108 89 22 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3092 3851 4424 6866 9337 9711 Size: 1064 138 1503 759 573 2442 2471 374 Cuts at: 9711 9857 387 Size: 146 1280 Fragments arranged by size: 2471 2442 1503 1280 1064 759 573 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 5449 5939 2227 Size: 3222 490 7038 Fragments arranged by size: 7038 3222 490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2732 3438 3642 4232 4813 4865 Size: 393 632 468 706 204 590 581 52 Cuts at: 4865 5305 5474 5529 5777 6028 6277 7309 7337 Size: 440 169 55 248 251 249 1032 28 Cuts at: 7337 7634 7876 7949 8813 9410 10197 10465 1239 Size: 297 242 73 864 597 787 268 1524 Fragments arranged by size: 1524 1032 864 787 706 632 597 590 581 468 440 393 297 268 251 249 248 242 204 169 73 55 52 28 EcoNI CCTnn'n_nnAGG Cuts at: 3601 5474 6239 7361 7738 3601 Size: 1873 765 1122 377 6613 Fragments arranged by size: 6613 1873 1122 765 377 EcoO109I rG'GnC_Cy Cuts at: 2248 2575 2680 2727 2728 3204 3343 3921 3988 Size: 327 105 47 1 476 139 578 67 Cuts at: 3988 4909 5263 5280 5374 5488 5557 5891 7582 Size: 921 354 17 94 114 69 334 1691 Cuts at: 7582 7837 2248 Size: 255 5161 Fragments arranged by size: 5161 1691 921 578 476 354 334 327 255 139 114 105 94 69 67 47 17 1 EcoRI G'AATT_C Cuts at: 4235 8059 8437 4235 Size: 3824 378 6548 Fragments arranged by size: 6548 3824 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 5815 6604 6865 2326 Size: 3489 789 261 6211 Fragments arranged by size: 6211 3489 789 261 FspI TGC'GCA Cuts at: 2849 5585 9256 2849 Size: 2736 3671 4343 Fragments arranged by size: 4343 3671 2736 HaeII r_GCGC'y Cuts at: 1678 2864 3133 3304 4084 4211 5527 5666 5991 Size: 1186 269 171 780 127 1316 139 325 Cuts at: 5991 9897 1678 Size: 3906 2531 Fragments arranged by size: 3906 2531 1316 1186 780 325 269 171 139 127 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2521 2553 3356 3388 3824 Size: 32 135 32 949 32 803 32 436 Cuts at: 3824 3856 4604 4636 4686 4718 5204 5236 5393 Size: 32 748 32 50 32 486 32 157 Cuts at: 5393 5425 7448 7480 8024 8056 9391 9423 9465 Size: 32 2023 32 544 32 1335 32 42 Cuts at: 9465 9497 1373 Size: 32 2626 Fragments arranged by size: 2626 2023 1335 949 803 748 544 486 436 157 135 50 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6202 7294 8094 8340 669 Size: 988 281 4264 1092 800 246 3079 Fragments arranged by size: 4264 3079 1092 988 800 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 10750 HpaI GTT'AAC Cuts at: 8340 8340 Size: 10750 KasI G'GCGC_C Cuts at: 5987 5987 Size: 10750 KpnI G_GTAC'C Cuts at: 6229 8075 6229 Size: 1846 8904 MfeI C'AATT_G Cuts at: 8349 8349 Size: 10750 MluI A'CGCG_T Cuts at: 2556 10626 2556 Size: 8070 2680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4237 4360 9864 10048 1305 Size: 2932 123 5504 184 2007 Fragments arranged by size: 5504 2932 2007 184 123 MscI TGG'CCA Cuts at: 10 64 1489 1832 3756 4460 5864 6683 6707 Size: 54 1425 343 1924 704 1404 819 24 Cuts at: 6707 7031 7334 10 Size: 324 303 3426 Fragments arranged by size: 3426 1924 1425 1404 819 704 343 324 303 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 3320 4175 4550 4727 5153 5639 5963 Size: 648 2154 855 375 177 426 486 324 Cuts at: 5963 6056 6131 6458 6602 6638 7511 8708 9067 Size: 93 75 327 144 36 873 1197 359 Cuts at: 9067 9226 518 Size: 159 2042 Fragments arranged by size: 2154 2042 1197 873 855 648 486 426 375 359 327 324 177 159 144 93 75 36 MspA1I CmG'CkG Cuts at: 1606 2106 2170 3582 3656 3985 4108 4885 4966 Size: 500 64 1412 74 329 123 777 81 Cuts at: 4966 5005 5013 5444 5616 6284 6771 7803 8166 Size: 39 8 431 172 668 487 1032 363 Cuts at: 8166 8843 9991 10236 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1412 1148 1032 777 677 668 500 487 431 363 329 245 172 123 81 74 64 39 8 NaeI GCC'GGC Cuts at: 7135 7135 Size: 10750 NarI GG'CG_CC Cuts at: 5988 5988 Size: 10750 NcoI C'CATG_G Cuts at: 513 1065 2004 2579 3315 3766 7524 7716 513 Size: 552 939 575 736 451 3758 192 3547 Fragments arranged by size: 3758 3547 939 736 575 552 451 192 NdeI CA'TA_TG Cuts at: 387 5743 387 Size: 5356 5394 NgoMIV G'CCGG_C Cuts at: 7133 7133 Size: 10750 NheI G'CTAG_C Cuts at: 1052 4540 1052 Size: 3488 7262 NotI GC'GGCC_GC Cuts at: 8197 8197 Size: 10750 NspI r_CATG'y Cuts at: 1132 1229 2410 3009 4579 6340 7075 7105 7300 Size: 97 1181 599 1570 1761 735 30 195 Cuts at: 7300 7772 8108 9653 1132 Size: 472 336 1545 2229 Fragments arranged by size: 2229 1761 1570 1545 1181 735 599 472 336 195 97 30 PasI CC'CwG_GG Cuts at: 1348 3721 4996 5378 5561 5872 1348 Size: 2373 1275 382 183 311 6226 Fragments arranged by size: 6226 2373 1275 382 311 183 PciI A'CATG_T Cuts at: 3005 6336 7071 7296 9649 3005 Size: 3331 735 225 2353 4106 Fragments arranged by size: 4106 3331 2353 735 225 PflMI CCAn_nnn'nTGG Cuts at: 1108 4467 5283 5605 5811 6771 7552 1108 Size: 3359 816 322 206 960 781 4306 Fragments arranged by size: 4306 3359 960 816 781 322 206 PfoI T'CCnGG_A Cuts at: 3306 3736 5276 5451 7314 3306 Size: 430 1540 175 1863 6742 Fragments arranged by size: 6742 1863 1540 430 175 PmeI GTTT'AAAC Cuts at: 8054 8054 Size: 10750 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2948 2980 3733 3765 7619 7651 8819 8851 10332 Size: 32 753 32 3854 32 1168 32 1481 Cuts at: 10332 10364 2948 Size: 32 3334 Fragments arranged by size: 3854 3334 1481 1168 753 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2575 3204 5280 5488 7582 2248 Size: 327 629 2076 208 2094 5416 Fragments arranged by size: 5416 2094 2076 629 327 208 PshAI GACnn'nnGTC Cuts at: 1613 6657 1613 Size: 5044 5706 PsiI TTA'TAA Cuts at: 8320 8320 Size: 10750 PspOMI G'GGCC_C Cuts at: 2679 2727 3920 3987 5890 2679 Size: 48 1193 67 1903 7539 Fragments arranged by size: 7539 1903 1193 67 48 PspXI vC'TCGA_Gb Cuts at: 2538 2538 Size: 10750 PstI C_TGCA'G Cuts at: 830 2181 2814 4254 6243 8102 830 Size: 1351 633 1440 1989 1859 3478 Fragments arranged by size: 3478 1989 1859 1440 1351 633 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10750 PvuII CAG'CTG Cuts at: 2170 4885 5005 5013 5616 6284 6771 2170 Size: 2715 120 8 603 668 487 6149 Fragments arranged by size: 6149 2715 668 603 487 120 8 SacI G_AGCT'C Cuts at: 2229 5451 5941 2229 Size: 3222 490 7038 Fragments arranged by size: 7038 3222 490 SacII CC_GC'GG Cuts at: 2107 3583 2107 Size: 1476 9274 SalI G'TCGA_C Cuts at: 1936 8092 1936 Size: 6156 4594 SanDI GG'GwC_CC Cuts at: 2575 2575 Size: 10750 SapI GCTCTTCn'nnn_ Cuts at: 2405 2822 6300 6869 6998 2405 Size: 417 3478 569 129 6157 Fragments arranged by size: 6157 3478 569 417 129 SbfI CC_TGCA'GG Cuts at: 2181 8102 2181 Size: 5921 4829 ScaI AGT'ACT Cuts at: 5798 6482 6845 8998 10710 5798 Size: 684 363 2153 1712 5838 Fragments arranged by size: 5838 2153 1712 684 363 SexAI A'CCwGG_T Cuts at: 3213 4263 3213 Size: 1050 9700 SfcI C'TryA_G Cuts at: 826 1045 2177 2263 2810 4250 6239 7984 8098 Size: 219 1132 86 547 1440 1989 1745 114 Cuts at: 8098 9233 9914 10105 826 Size: 1135 681 191 1471 Fragments arranged by size: 1989 1745 1471 1440 1135 1132 681 547 219 191 114 86 SfoI GGC'GCC Cuts at: 5989 5989 Size: 10750 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10750 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10750 SmaI CCC'GGG Cuts at: 1872 5122 8077 1872 Size: 3250 2955 4545 Fragments arranged by size: 4545 3250 2955 SmlI C'TyrA_G Cuts at: 820 1017 1943 2538 2951 3326 5717 5768 8854 Size: 197 926 595 413 375 2391 51 3086 Cuts at: 8854 9755 10017 820 Size: 901 262 1553 Fragments arranged by size: 3086 2391 1553 926 901 595 413 375 262 197 51 SnaBI TAC'GTA Cuts at: 493 8541 493 Size: 8048 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10750 SphI G_CATG'C Cuts at: 1132 4579 7105 7772 8108 1132 Size: 3447 2526 667 336 3774 Fragments arranged by size: 3774 3447 2526 667 336 SspI AAT'ATT Cuts at: 5 52 8674 5 Size: 47 8622 2081 Fragments arranged by size: 8622 2081 47 StuI AGG'CCT Cuts at: 1517 2183 4626 6587 1517 Size: 666 2443 1961 5680 Fragments arranged by size: 5680 2443 1961 666 StyI C'CwwG_G Cuts at: 513 1065 2004 2579 3315 3384 3766 6189 6921 Size: 552 939 575 736 69 382 2423 732 Cuts at: 6921 7524 7716 7879 8189 513 Size: 603 192 163 310 3074 Fragments arranged by size: 3074 2423 939 736 732 603 575 552 382 310 192 163 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 3299 5764 7426 8777 8794 8947 Size: 1255 705 1014 2465 1662 1351 17 153 Cuts at: 8947 9132 9577 325 Size: 185 445 1498 Fragments arranged by size: 2465 1662 1498 1351 1255 1014 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 5796 6180 Size: 274 80 33 51 1443 510 3309 384 Cuts at: 6180 6339 6480 6843 7299 8996 10708 96 Size: 159 141 363 456 1697 1712 138 Fragments arranged by size: 3309 1712 1697 1443 510 456 384 363 274 159 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5902 5934 10710 10742 5902 Size: 32 4776 32 5910 Fragments arranged by size: 5910 4776 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7937 1316 Size: 6621 4129 XbaI T'CTAG_A Cuts at: 8086 8086 Size: 10750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 3358 3764 5568 6499 7024 7582 2881 Size: 477 406 1804 931 525 558 6049 Fragments arranged by size: 6049 1804 931 558 525 477 406 XhoI C'TCGA_G Cuts at: 1943 2538 1943 Size: 595 10155 XmaI C'CCGG_G Cuts at: 1870 5120 8075 1870 Size: 3250 2955 4545 Fragments arranged by size: 4545 3250 2955 XmnI GAAnn'nnTTC Cuts at: 4352 4802 5798 6779 6845 8879 4352 Size: 450 996 981 66 2034 6223 Fragments arranged by size: 6223 2034 996 981 450 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5842 6595 8558 276 Size: 53 83 186 936 4308 753 1963 2468 Fragments arranged by size: 4308 2468 1963 936 753 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SfiI SrfI SwaI