################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BlnI BsaAI BsgI BsiEI BsiWI BsmBI BspDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HpaI KpnI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I StuI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccIII 1187 TCCGGA T^CCGGA AcyI 46 GGCGCC G[GA]^CG[CT]C AflIII 831 ACATGT A^C[GA][CT]GT AhdI 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1783 CCTCAGG CC^T[ACGT]AGG AspEI 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 46 GGCGCC GGCGC^C BglI 844 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1277 AGATCT A^GATCT BlpI 2067 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2067 GCTCAGC GC^T[ACGT]AGC BsaBI 666 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 46 GGCGCC G[GA]^CG[CT]C BsaWI 1187 TCCGGA [AT]^CCGG[AT] BseAI 1187 TCCGGA T^CCGGA BsmI 2007 GAATGC GAATGC(1/-1) Bsp1407I 1052 TGTACA T^GTACA BspEI 1187 TCCGGA T^CCGGA BspHI 437 TCATGA T^CATGA BspLU11I 831 ACATGT A^CATGT BsrBI 402 GAGCGG CCGCTC(-3/-3) BsrDI 197 GCAATG GCAATG(2/0) BsrFI 1824 GCCGGT [GA]^CCGG[CT] BsrGI 1052 TGTACA T^GTACA BstBI 980 TTCGAA TT^CGAA BstXI 56 CCAAACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1783 CCTCAGG CC^T[ACGT]AGG CelII 2067 GCTCAGC GC^T[ACGT]AGC Cfr10I 1824 GCCGGT [GA]^CCGG[CT] DraII 1675 GGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 457 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1774 GAAGAG CTCTTC(1/4) Eco81I 1783 CCTCAGG CC^T[ACGT]AGG EcoNI 1679 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1675 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1834 GATATC GAT^ATC HaeII 46 GGCGCC [GA]GCGC^[CT] Hin1I 46 GGCGCC G[GA]^CG[CT]C HincII 1093 GTCAAC GT[CT]^[GA]AC HindII 1093 GTCAAC GT[CT]^[GA]AC HindIII 1072 AAGCTT A^AGCTT KasI 46 GGCGCC G^GCGCC Ksp632I 1774 GAAGAG CTCTTC(1/4) KspI 11 CCGCGG CCGC^GG MaeII 343 ACGT A^CGT MamI 666 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1187 TCCGGA T^CCGGA NarI 46 GGCGCC GG^CGCC NdeI 1870 CATATG CA^TATG NspV 980 TTCGAA TT^CGAA PaeR7I 282 CTCGAG C^TCGAG PpuMI 1675 GGGACCT [GA]G^G[AT]CC[CT] PvuII 877 CAGCTG CAG^CTG RcaI 437 TCATGA T^CATGA SacII 11 CCGCGG CCGC^GG ScaI 1982 AGTACT AGT^ACT SfoI 46 GGCGCC GGC^GCC SfuI 980 TTCGAA TT^CGAA SmaI 332 CCCGGG CCC^GGG SphI 156 GCATGC GCATG^C SspBI 1052 TGTACA T^GTACA XhoI 282 CTCGAG C^TCGAG XmaCI 332 CCCGGG C^CCGGG XmaI 332 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 1381 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1519 CAGAACCTG AseI 739 ATTAAT AT^TAAT 1084 ATTAAT AsnI 739 ATTAAT AT^TAAT 1084 ATTAAT AspI 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2161 GACTTCGTC BanI 46 GGCGCC G^G[CT][GA]CC 1885 GGTGCC BanII 842 GAGCCC G[GA]GC[CT]^C 2146 GAGCCC BciVI 519 GGATAC GTATCC(6/5) 1376 GTATCC BclI 72 TGATCA T^GATCA 1640 TGATCA BfaI 1301 CTAG C^TAG 1487 CTAG BpmI 151 CTCCAG CTGGAG(16/14) 239 CTCCAG BsaI 1306 GAGACC GGTCTC(1/5) 122 GGTCTC FbaI 72 TGATCA T^GATCA 1640 TGATCA HgaI 2043 GACGC GACGC(5/10) 2122 GACGC MaeI 1301 CTAG C^TAG 1487 CTAG MslI 387 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1171 CACCAAGATG NcoI 501 CCATGG C^CATGG 1103 CCATGG PflFI 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2161 GACTTCGTC PflMI 32 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 648 CCAGAAAGTGG PshBI 739 ATTAAT AT^TAAT 1084 ATTAAT PstI 592 CTGCAG CTGCA^G 1516 CTGCAG SfcI 592 CTGCAG C^T[GA][CT]AG 1516 CTGCAG SmlI 282 CTCGAG C^T[CT][GA]AG 1198 CTTGAG SspI 140 AATATT AAT^ATT 790 AATATT Tsp45I 340 GTCAC ^GT[GC]AC 1849 GTGAC Tth111I 16 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2161 GACTTCGTC Van91I 32 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 648 CCAGAAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII