(Circular) (Six-base) MAPSORT of: pFN21AE2331.seq Check: 5278 from: 1 to: 6886 pFN21AE2331 6886 bp With 182 enzymes: SgfI * March 30, 2011 18:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2706 3439 2706 Size: 733 6153 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 4185 5013 5386 1563 Size: 2622 828 373 3063 Fragments arranged by size: 3063 2622 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 2780 3447 4949 6333 6601 1632 Size: 704 444 667 1502 1384 268 1917 Fragments arranged by size: 1917 1502 1384 704 667 444 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 2836 5785 6762 1203 Size: 1633 2949 977 1327 Fragments arranged by size: 2949 1633 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 2467 5615 2467 Size: 3148 3738 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 3391 3529 4629 6201 3391 Size: 138 1100 1572 4076 Fragments arranged by size: 4076 1572 1100 138 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 4195 4421 4573 6682 1493 Size: 2702 226 152 2109 1697 Fragments arranged by size: 2702 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2745 3090 5440 160 Size: 872 1713 345 2350 1606 Fragments arranged by size: 2350 1713 1606 872 345 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2287 2337 4022 4211 1870 Size: 73 344 50 1685 189 4545 Fragments arranged by size: 4545 1685 344 189 73 50 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 3890 4207 5662 618 Size: 325 217 627 264 1839 317 1455 1842 Fragments arranged by size: 1842 1839 1455 627 325 317 264 217 BanII G_rGCy'C Cuts at: 1391 2851 4155 4205 1391 Size: 1460 1304 50 4072 Fragments arranged by size: 4072 1460 1304 50 BbeI G_GCGC'C Cuts at: 2055 2055 Size: 6886 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2928 3228 3435 928 Size: 971 1029 300 207 4379 Fragments arranged by size: 4379 1029 971 300 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5077 5111 1903 Size: 34 50 34 3056 34 3678 Fragments arranged by size: 3678 3056 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2518 3392 4778 5988 2518 Size: 874 1386 1210 3416 Fragments arranged by size: 3416 1386 1210 874 BclI T'GATC_A Cuts at: 1548 2077 3645 1548 Size: 529 1568 4789 Fragments arranged by size: 4789 1568 529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2855 5497 136 Size: 107 122 71 631 605 1183 2642 1525 Fragments arranged by size: 2642 1525 1183 631 605 122 107 71 BglII A'GATC_T Cuts at: 3282 6881 3282 Size: 3599 3287 BlpI GC'TnA_GC Cuts at: 4073 4303 4073 Size: 230 6656 Bme1580I G_kGCm'C Cuts at: 1790 3804 3895 4947 6103 1790 Size: 2014 91 1052 1156 2573 Fragments arranged by size: 2573 2014 1156 1052 91 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4617 5575 442 Size: 416 53 83 399 24 3200 958 1753 Fragments arranged by size: 3200 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2229 5546 1239 Size: 902 88 3317 2579 Fragments arranged by size: 3317 2579 902 88 Bpu10I CC'TnA_GC Cuts at: 3530 3530 Size: 6886 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3224 5011 5876 6174 3224 Size: 1787 865 298 3936 Fragments arranged by size: 3936 1787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2133 3305 5549 882 Size: 617 634 1172 2244 2219 Fragments arranged by size: 2244 2219 1172 634 617 BsaAI yAC'GTr Cuts at: 493 1204 4677 493 Size: 711 3473 2702 Fragments arranged by size: 3473 2702 711 BsaBI GATnn'nnATC Cuts at: 2675 6880 2675 Size: 4205 2681 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 4693 5075 5710 Size: 53 83 186 936 519 2641 382 635 Cuts at: 5710 275 Size: 1451 Fragments arranged by size: 2641 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3192 5319 5717 5991 6138 6549 3192 Size: 2127 398 274 147 411 3529 Fragments arranged by size: 3529 2127 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2227 2257 3109 3139 1178 Size: 30 165 30 824 30 852 30 4925 Fragments arranged by size: 4925 852 824 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2791 3377 1186 Size: 1192 413 586 4695 Fragments arranged by size: 4695 1192 586 413 BseYI C'CCAG_C Cuts at: 2259 2850 4154 6089 6585 2259 Size: 591 1304 1935 496 2560 Fragments arranged by size: 2560 1935 1304 591 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6313 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2634 2904 3478 4947 5032 6103 2634 Size: 270 574 1469 85 1071 3417 Fragments arranged by size: 3417 1469 1071 574 270 85 BsmI GAATG_Cn' Cuts at: 4018 4397 4490 4018 Size: 379 93 6414 Fragments arranged by size: 6414 379 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2634 2851 2904 3478 3804 3895 4155 Size: 399 844 217 53 574 326 91 260 Cuts at: 4155 4205 4947 5032 6103 1391 Size: 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 844 742 574 399 326 260 217 91 85 53 50 BspEI T'CCGG_A Cuts at: 3192 6549 3192 Size: 3357 3529 BspHI T'CATG_A Cuts at: 2442 4775 6505 2442 Size: 2333 1730 2823 Fragments arranged by size: 2823 2333 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 3323 3439 3454 3692 4241 844 Size: 1862 617 116 15 238 549 3489 Fragments arranged by size: 3489 1862 617 549 238 116 15 BsrBI CCG'CTC Cuts at: 1394 1823 2409 4773 1394 Size: 429 586 2364 3507 Fragments arranged by size: 3507 2364 586 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2209 5381 5555 65 Size: 1183 961 3172 174 1396 Fragments arranged by size: 3172 1396 1183 961 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3829 5530 5717 1700 Size: 110 2019 1701 187 2869 Fragments arranged by size: 2869 2019 1701 187 110 BsrGI T'GTAC_A Cuts at: 96 3057 96 Size: 2961 3925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 4946 5958 4946 Size: 1012 5874 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6886 BstBI TT'CG_AA Cuts at: 2986 5690 2986 Size: 2704 4182 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2068 Size: 6886 BstYI r'GATC_y Cuts at: 1972 3282 3353 3543 4119 4216 4583 4968 4985 Size: 1310 71 190 576 97 367 385 17 Cuts at: 4985 6437 6523 6535 6881 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1310 576 385 367 346 190 97 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3789 3789 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 2016 2506 3108 3849 513 Size: 552 951 490 602 741 3550 Fragments arranged by size: 3550 951 741 602 552 490 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3952 487 Size: 683 56 2726 3421 Fragments arranged by size: 3421 2726 683 56 BtsI GCAGTG_nn' Cuts at: 2745 3034 4402 5196 5216 2745 Size: 289 1368 794 20 4415 Fragments arranged by size: 4415 1368 794 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3010 3045 529 Size: 35 2446 35 4370 Fragments arranged by size: 4370 2446 35 35 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2045 2325 Size: 54 1425 50 275 18 204 11 280 Cuts at: 2325 2610 2680 3244 4333 5222 6851 8 Size: 285 70 564 1089 889 1629 43 Fragments arranged by size: 1629 1425 1089 889 564 285 280 275 204 70 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3774 4816 1353 Size: 2421 1042 3423 Fragments arranged by size: 3423 2421 1042 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3545 3724 4082 4125 5473 5847 Size: 1064 138 1956 179 358 43 1348 374 Cuts at: 5847 5993 387 Size: 146 1280 Fragments arranged by size: 1956 1348 1280 1064 374 358 179 146 138 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2229 2336 2780 3447 4949 5546 Size: 393 509 88 107 444 667 1502 597 Cuts at: 5546 6333 6601 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1502 787 667 597 509 444 393 268 107 88 EcoNI CCTnn'n_nnAGG Cuts at: 3688 3688 Size: 6886 EcoO109I rG'GnC_Cy Cuts at: 3681 3681 Size: 6886 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 EcoRV GAT'ATC Cuts at: 3841 3841 Size: 6886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2470 3012 3911 2470 Size: 542 899 5445 Fragments arranged by size: 5445 899 542 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 2055 6033 1678 Size: 377 3978 2531 Fragments arranged by size: 3978 2531 377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2366 2398 2453 Size: 32 135 32 539 32 223 32 55 Cuts at: 2453 2485 2993 3025 3127 3159 3575 3607 5527 Size: 32 508 32 102 32 416 32 1920 Cuts at: 5527 5559 5601 5633 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1920 539 508 416 223 135 102 55 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3100 4230 4476 669 Size: 988 281 1162 1130 246 3079 Fragments arranged by size: 3079 1162 1130 988 281 246 HindIII A'AGCT_T Cuts at: 748 3077 748 Size: 2329 4557 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KasI G'GCGC_C Cuts at: 2051 2051 Size: 6886 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6000 6184 1305 Size: 4695 184 2007 Fragments arranged by size: 4695 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2327 2612 10 Size: 54 1425 343 204 291 285 4284 Fragments arranged by size: 4284 1425 343 291 285 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3180 4844 5203 5362 518 Size: 648 1230 784 1664 359 159 2042 Fragments arranged by size: 2042 1664 1230 784 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2602 2854 2884 4302 4979 6127 6372 Size: 412 584 252 30 1418 677 1148 245 Cuts at: 6372 1606 Size: 2120 Fragments arranged by size: 2120 1418 1148 677 584 412 252 245 30 NarI GG'CG_CC Cuts at: 2052 2052 Size: 6886 NcoI C'CATG_G Cuts at: 513 1065 2506 3108 513 Size: 552 1441 602 4291 Fragments arranged by size: 4291 1441 602 552 NdeI CA'TA_TG Cuts at: 387 3876 387 Size: 3489 3397 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 2165 2467 2840 3157 4244 5789 1132 Size: 97 936 302 373 317 1087 1545 2229 Fragments arranged by size: 2229 1545 1087 936 373 317 302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 2836 5785 2836 Size: 2949 3937 PflMI CCAn_nnn'nTGG Cuts at: 1108 2043 2659 1108 Size: 935 616 5335 Fragments arranged by size: 5335 935 616 PfoI T'CCnGG_A Cuts at: 2916 4098 2916 Size: 1182 5704 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3681 3681 Size: 6886 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 2366 3072 4456 2366 Size: 706 1384 4796 Fragments arranged by size: 4796 1384 706 PspXI vC'TCGA_Gb Cuts at: 2287 2287 Size: 6886 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3518 3550 3518 Size: 32 6854 PstI C_TGCA'G Cuts at: 830 2601 3525 4238 830 Size: 1771 924 713 3478 Fragments arranged by size: 3478 1771 924 713 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 2884 2884 Size: 6886 SacII CC_GC'GG Cuts at: 2019 2019 Size: 6886 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 3989 5134 6846 3989 Size: 1145 1712 4029 Fragments arranged by size: 4029 1712 1145 SfcI C'TryA_G Cuts at: 826 1045 2597 3521 4234 5369 6050 6241 826 Size: 219 1552 924 713 1135 681 191 1471 Fragments arranged by size: 1552 1471 1135 924 713 681 219 191 SfoI GGC'GCC Cuts at: 2053 2053 Size: 6886 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 2339 4213 1872 Size: 467 1874 4545 Fragments arranged by size: 4545 1874 467 SmlI C'TyrA_G Cuts at: 820 1017 1943 2287 3203 4990 5891 6153 820 Size: 197 926 344 916 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 916 901 344 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 2165 4244 1132 Size: 1033 2079 3774 Fragments arranged by size: 3774 2079 1033 SspI AAT'ATT Cuts at: 5 52 2147 2797 4810 5 Size: 47 2095 650 2013 2081 Fragments arranged by size: 2095 2081 2013 650 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2506 2607 3108 4146 4325 513 Size: 552 1441 101 501 1038 179 3074 Fragments arranged by size: 3074 1441 1038 552 501 179 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2112 3359 4913 4930 5083 5268 5713 Size: 1255 532 1247 1554 17 153 185 445 Cuts at: 5713 325 Size: 1498 Fragments arranged by size: 1554 1498 1255 1247 532 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3057 3159 3987 Size: 274 80 33 51 1443 1080 102 828 Cuts at: 3987 5132 6844 96 Size: 1145 1712 138 Fragments arranged by size: 1712 1443 1145 1080 828 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2826 2858 3109 3141 3338 3370 6846 6878 2826 Size: 32 251 32 197 32 3476 32 2834 Fragments arranged by size: 3476 2834 251 197 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2024 4169 1316 Size: 708 2145 4033 Fragments arranged by size: 4033 2145 708 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XhoI C'TCGA_G Cuts at: 1943 2287 1943 Size: 344 6542 XmaI C'CCGG_G Cuts at: 1870 2337 4211 1870 Size: 467 1874 4545 Fragments arranged by size: 4545 1874 467 XmnI GAAnn'nnTTC Cuts at: 5015 5015 Size: 6886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI