(Linear) (Six-base) MAPSORT of: KIEE2331.seq Check: 3615 from: 1 to: 2181 KIEE2331.orf With 182 enzymes: SgfI * March 30, 2011 17:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 701 1434 2181 Size: 701 733 747 Fragments arranged by size: 747 733 701 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 775 1442 2181 Size: 331 444 667 739 Fragments arranged by size: 739 667 444 331 AflIII A'CryG_T Cuts at: 0 831 2181 Size: 831 1350 AhdI GACnn_n'nnGTC Cuts at: 0 462 2181 Size: 462 1719 AlwNI CAG_nnn'CTG Cuts at: 0 1386 1524 2181 Size: 1386 138 657 Fragments arranged by size: 1386 657 138 AseI AT'TA_AT Cuts at: 0 740 1085 2181 Size: 740 345 1096 Fragments arranged by size: 1096 740 345 AvaI C'yCGr_G Cuts at: 0 282 332 2017 2181 Size: 282 50 1685 164 Fragments arranged by size: 1685 282 164 50 BanI G'GyrC_C Cuts at: 0 46 1885 2181 Size: 46 1839 296 Fragments arranged by size: 1839 296 46 BanII G_rGCy'C Cuts at: 0 846 2150 2181 Size: 846 1304 31 Fragments arranged by size: 1304 846 31 BbeI G_GCGC'C Cuts at: 0 50 2181 Size: 50 2131 BbsI GAAGACnn'nnnn_ Cuts at: 0 923 1223 1430 2181 Size: 923 300 207 751 Fragments arranged by size: 923 751 300 207 BciVI GTATCCnnnnn_n' Cuts at: 0 513 1387 2181 Size: 513 874 794 Fragments arranged by size: 874 794 513 BclI T'GATC_A Cuts at: 0 72 1640 2181 Size: 72 1568 541 Fragments arranged by size: 1568 541 72 BglI GCCn_nnn'nGGC Cuts at: 0 850 2181 Size: 850 1331 BglII A'GATC_T Cuts at: 0 1277 2181 Size: 1277 904 BlpI GC'TnA_GC Cuts at: 0 2068 2181 Size: 2068 113 Bme1580I G_kGCm'C Cuts at: 0 1799 1890 2181 Size: 1799 91 291 Fragments arranged by size: 1799 291 91 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 224 2181 Size: 136 88 1957 Fragments arranged by size: 1957 136 88 Bpu10I CC'TnA_GC Cuts at: 0 1525 2181 Size: 1525 656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1219 2181 Size: 1219 962 BsaI GGTCTCn'nnnn_ Cuts at: 0 128 1300 2181 Size: 128 1172 881 Fragments arranged by size: 1172 881 128 BsaBI GATnn'nnATC Cuts at: 0 670 2181 Size: 670 1511 BsaHI Gr'CG_yC Cuts at: 0 47 2181 Size: 47 2134 BsaWI w'CCGG_w Cuts at: 0 1187 2181 Size: 1187 994 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 222 252 1104 1134 2181 Size: 222 30 852 30 1047 Fragments arranged by size: 1047 852 222 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 373 786 1372 2181 Size: 373 413 586 809 Fragments arranged by size: 809 586 413 373 BseYI C'CCAG_C Cuts at: 0 254 845 2149 2181 Size: 254 591 1304 32 Fragments arranged by size: 1304 591 254 32 BsiHKAI G_wGCw'C Cuts at: 0 629 899 1473 2181 Size: 629 270 574 708 Fragments arranged by size: 708 629 574 270 BsmI GAATG_Cn' Cuts at: 0 2013 2181 Size: 2013 168 Bsp1286I G_dGCh'C Cuts at: 0 629 846 899 1473 1799 1890 2150 2181 Size: 629 217 53 574 326 91 260 31 Fragments arranged by size: 629 574 326 260 217 91 53 31 BspEI T'CCGG_A Cuts at: 0 1187 2181 Size: 1187 994 BspHI T'CATG_A Cuts at: 0 437 2181 Size: 437 1744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 701 1318 1434 1449 1687 2181 Size: 701 617 116 15 238 494 Fragments arranged by size: 701 617 494 238 116 15 BsrBI CCG'CTC Cuts at: 0 404 2181 Size: 404 1777 BsrDI GCAATG_nn' Cuts at: 0 204 2181 Size: 204 1977 BsrFI r'CCGG_y Cuts at: 0 1824 2181 Size: 1824 357 BsrGI T'GTAC_A Cuts at: 0 1052 2181 Size: 1052 1129 BstBI TT'CG_AA Cuts at: 0 981 2181 Size: 981 1200 BstXI CCAn_nnnn'nTGG Cuts at: 0 63 2181 Size: 63 2118 BstYI r'GATC_y Cuts at: 0 1277 1348 1538 2114 2181 Size: 1277 71 190 576 67 Fragments arranged by size: 1277 576 190 71 67 Bsu36I CC'TnA_GG Cuts at: 0 1784 2181 Size: 1784 397 BtgI C'CryG_G Cuts at: 0 11 501 1103 1844 2181 Size: 11 490 602 741 337 Fragments arranged by size: 741 602 490 337 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1947 2181 Size: 1947 234 BtsI GCAGTG_nn' Cuts at: 0 740 1029 2181 Size: 740 289 1152 Fragments arranged by size: 1152 740 289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1005 1040 2181 Size: 1005 35 1141 Fragments arranged by size: 1141 1005 35 EaeI y'GGCC_r Cuts at: 0 29 40 320 605 675 1239 2181 Size: 29 11 280 285 70 564 942 Fragments arranged by size: 942 564 285 280 70 29 11 EarI CTCTTCn'nnn_ Cuts at: 0 1769 2181 Size: 1769 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1540 1719 2077 2120 2181 Size: 1540 179 358 43 61 Fragments arranged by size: 1540 358 179 61 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 224 331 775 1442 2181 Size: 136 88 107 444 667 739 Fragments arranged by size: 739 667 444 136 107 88 EcoNI CCTnn'n_nnAGG Cuts at: 0 1683 2181 Size: 1683 498 EcoO109I rG'GnC_Cy Cuts at: 0 1676 2181 Size: 1676 505 EcoRV GAT'ATC Cuts at: 0 1836 2181 Size: 1836 345 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 465 1007 1906 2181 Size: 465 542 899 275 Fragments arranged by size: 899 542 465 275 HaeII r_GCGC'y Cuts at: 0 50 2181 Size: 50 2131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 361 393 448 480 988 1020 Size: 106 32 223 32 55 32 508 32 Cuts at: 1020 1122 1154 1570 1602 2181 Size: 102 32 416 32 579 Fragments arranged by size: 579 508 416 223 106 102 55 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1095 2181 Size: 1095 1086 HindIII A'AGCT_T Cuts at: 0 1072 2181 Size: 1072 1109 KasI G'GCGC_C Cuts at: 0 46 2181 Size: 46 2135 MscI TGG'CCA Cuts at: 0 31 322 607 2181 Size: 31 291 285 1574 Fragments arranged by size: 1574 291 285 31 MslI CAynn'nnrTG Cuts at: 0 391 1175 2181 Size: 391 784 1006 Fragments arranged by size: 1006 784 391 MspA1I CmG'CkG Cuts at: 0 13 597 849 879 2181 Size: 13 584 252 30 1302 Fragments arranged by size: 1302 584 252 30 13 NarI GG'CG_CC Cuts at: 0 47 2181 Size: 47 2134 NcoI C'CATG_G Cuts at: 0 501 1103 2181 Size: 501 602 1078 Fragments arranged by size: 1078 602 501 NdeI CA'TA_TG Cuts at: 0 1871 2181 Size: 1871 310 NspI r_CATG'y Cuts at: 0 160 462 835 1152 2181 Size: 160 302 373 317 1029 Fragments arranged by size: 1029 373 317 302 160 PciI A'CATG_T Cuts at: 0 831 2181 Size: 831 1350 PflMI CCAn_nnn'nTGG Cuts at: 0 38 654 2181 Size: 38 616 1527 Fragments arranged by size: 1527 616 38 PfoI T'CCnGG_A Cuts at: 0 911 2093 2181 Size: 911 1182 88 Fragments arranged by size: 1182 911 88 PpuMI rG'GwC_Cy Cuts at: 0 1676 2181 Size: 1676 505 PsiI TTA'TAA Cuts at: 0 361 1067 2181 Size: 361 706 1114 Fragments arranged by size: 1114 706 361 PspXI vC'TCGA_Gb Cuts at: 0 282 2181 Size: 282 1899 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1513 1545 2181 Size: 1513 32 636 Fragments arranged by size: 1513 636 32 PstI C_TGCA'G Cuts at: 0 596 1520 2181 Size: 596 924 661 Fragments arranged by size: 924 661 596 PvuII CAG'CTG Cuts at: 0 879 2181 Size: 879 1302 SacII CC_GC'GG Cuts at: 0 14 2181 Size: 14 2167 ScaI AGT'ACT Cuts at: 0 1984 2181 Size: 1984 197 SfcI C'TryA_G Cuts at: 0 592 1516 2181 Size: 592 924 665 Fragments arranged by size: 924 665 592 SfoI GGC'GCC Cuts at: 0 48 2181 Size: 48 2133 SmaI CCC'GGG Cuts at: 0 334 2181 Size: 334 1847 SmlI C'TyrA_G Cuts at: 0 282 1198 2181 Size: 282 916 983 Fragments arranged by size: 983 916 282 SphI G_CATG'C Cuts at: 0 160 2181 Size: 160 2021 SspI AAT'ATT Cuts at: 0 142 792 2181 Size: 142 650 1389 Fragments arranged by size: 1389 650 142 StyI C'CwwG_G Cuts at: 0 501 602 1103 2141 2181 Size: 501 101 501 1038 40 Fragments arranged by size: 1038 501 501 101 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 107 1354 2181 Size: 107 1247 827 Fragments arranged by size: 1247 827 107 TatI w'GTAC_w Cuts at: 0 1052 1154 1982 2181 Size: 1052 102 828 199 Fragments arranged by size: 1052 828 199 102 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 821 853 1104 1136 1333 1365 2181 Size: 821 32 251 32 197 32 816 Fragments arranged by size: 821 816 251 197 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 19 2164 2181 Size: 19 2145 17 Fragments arranged by size: 2145 19 17 XhoI C'TCGA_G Cuts at: 0 282 2181 Size: 282 1899 XmaI C'CCGG_G Cuts at: 0 332 2181 Size: 332 1849 Enzymes that do cut: AarI AcuI AflIII AhdI AlwNI AseI AvaI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NarI NcoI NdeI NspI PciI PflMI PfoI PpuMI PsiI PspXI PsrI PstI PvuII SacII ScaI SfcI SfoI SmaI SmlI SphI SspI StyI TaqII TatI TstI Tth111I XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BfrBI BmgBI BmrI BmtI BplI BsaAI BsgI BsiEI BsiWI BsmBI BssHII BssSI BstAPI BstEII BstZ17I ClaI DraI DraIII DrdI EagI EcoICRI EcoRI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PspOMI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI XbaI XcmI XmnI ZraI