################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BcgI BglI BlnI BseAI BsiWI BsmBI BspEI BspLU11I BssHII BstBI BstEII BstPI Bsu36I CpoI DraIII Eam1105I Eco81I EcoNI EcoO65I FseI FspI HpaI KasI KpnI MluI MroI NaeI NarI NgoMIV NruI NspV PaeR7I PmaCI PmeI PmlI PshAI Psp1406I RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 16 CGCG CG^CG AcyI 3810 GACGCC G[GA]^CG[CT]C AgeI 3149 ACCGGT A^CCGGT Aor51HI 315 AGCGCT AGC^GCT AspI 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 92 CCCGAG C^[CT]CG[GA]G BamHI 923 GGATCC G^GATCC BanI 3861 GGTGCC G^G[CT][GA]CC BbvCI 1111 GCTGAGG CCTCAGC(-5/-2) BclI 1248 TGATCA T^GATCA BcnI 4287 CCCGG CC^[GC]GG BglII 1872 AGATCT A^GATCT BlpI 4751 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4751 GCTCAGC GC^T[ACGT]AGC BsaAI 3644 TACGTA [CT]AC^GT[GA] BsaHI 3810 GACGCC G[GA]^CG[CT]C BsaI 2815 GGTCTC GGTCTC(1/5) BsoBI 92 CCCGAG C^[CT]CG[GA]G BspDI 646 ATCGAT AT^CGAT BsrBI 21 CCGCTC CCGCTC(-3/-3) BsrDI 561 CATTGC GCAATG(2/0) BsrFI 3149 ACCGGT [GA]^CCGG[CT] BssSI 3710 CACGAG CACGAG(-5/-1) Bst1107I 4826 GTATAC GTA^TAC BstAPI 556 GCATGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 16 CGCG CG^CG BstZ17I 4826 GTATAC GTA^TAC CelII 4751 GCTCAGC GC^T[ACGT]AGC CfoI 316 GCGC GCG^C Cfr10I 3149 ACCGGT [GA]^CCGG[CT] ClaI 646 ATCGAT AT^CGAT DraI 1425 TTTAAA TTT^AAA Eco47III 315 AGCGCT AGC^GCT FauI 78 CCCGC CCCGC(4/6) FbaI 1248 TGATCA T^GATCA HaeII 315 AGCGCT [GA]GCGC^[CT] HhaI 316 GCGC GCG^C Hin1I 3810 GACGCC G[GA]^CG[CT]C HinP1I 316 GCGC G^CGC KspI 15 CCGCGG CCGC^GG MvnI 16 CGCG CG^CG NciI 4287 CCCGG CC^[GC]GG NcoI 4232 CCATGG C^CATGG NheI 4756 GCTAGC G^CTAGC NotI 17 GCGGCCGC GC^GGCCGC PacI 1585 TTAATTAA TTAAT^TAA PflFI 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3149 ACCGGT A^CCGGT PvuI 8 CGATCG CGAT^CG SacII 15 CCGCGG CCGC^GG SapI 3893 GAAGAGC GCTCTTC(1/4) SnaBI 3644 TACGTA TAC^GTA SphI 556 GCATGC GCATG^C SspI 2698 AATATT AAT^ATT Tth111I 788 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 2168 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG 5066 CAGAGGCTG BciVI 1566 GGATAC GTATCC(6/5) 4926 GTATCC BsaBI 1525 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3418 GATTTTAATC BsaWI 2561 ACCGGA [AT]^CCGG[AT] 3149 ACCGGT BseRI 67 GAGGAG GAGGAG(10/8) 1030 GAGGAG BsgI 4986 CTGCAC GTGCAG(16/14) 1409 GTGCAG BsmI 468 GAATGC GAATGC(1/-1) 4019 GAATGC Bsp1407I 3456 TGTACA T^GTACA 5037 TGTACA BspHI 4139 TCATGA T^CATGA 4893 TCATGA BsrGI 3456 TGTACA T^GTACA 5037 TGTACA BstXI 848 CCAGTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2004 CCACCCTCATGG DsaI 15 CCGCGG C^C[GA][CT]GG 4232 CCATGG EagI 12 CGGCCG C^GGCCG 18 CGGCCG EarI 1846 GAAGAG CTCTTC(1/4) 3893 GAAGAG EclXI 12 CGGCCG C^GGCCG 18 CGGCCG Eco52I 12 CGGCCG C^GGCCG 18 CGGCCG EcoRI 2233 GAATTC G^AATTC 4479 GAATTC HgaI 3810 GACGC GACGC(5/10) 2616 GCGTC HindIII 193 AAGCTT A^AGCTT 4046 AAGCTT Ksp632I 1846 GAAGAG CTCTTC(1/4) 3893 GAAGAG MamI 1525 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3418 GATTTTAATC MfeI 1502 CAATTG C^AATTG 2479 CAATTG MunI 1502 CAATTG C^AATTG 2479 CAATTG NspI 556 GCATGC [GA]CATG^[CT] 2329 GCATGT PpuMI 1455 AGGACCT [GA]G^G[AT]CC[CT] 2896 GGGACCT PstI 2165 CTGCAG CTGCA^G 2181 CTGCAG PvuII 803 CAGCTG CAG^CTG 1109 CAGCTG RcaI 4139 TCATGA T^CATGA 4893 TCATGA SacI 3627 GAGCTC GAGCT^C 3696 GAGCTC SpeI 2655 ACTAGT A^CTAGT 3169 ACTAGT SspBI 3456 TGTACA T^GTACA 5037 TGTACA XbaI 3091 TCTAGA T^CTAGA 3970 TCTAGA XcmI 1766 CCAACCTGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4648 CCATATTCTGATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanII BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DrdI EaeI EcoO109I EcoRII EcoRV EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NsiI PflMI PleI PshBI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI