(Linear) (Six-base) MAPSORT of: KIEE2323.seq Check: 9174 from: 1 to: 5124 KIEE2323.orf With 182 enzymes: SgfI * January 27, 2011 13:24 .. AccI GT'mk_AC Cuts at: 0 978 2627 4827 5124 Size: 978 1649 2200 297 Fragments arranged by size: 2200 1649 978 297 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 571 827 1865 2324 4064 5124 Size: 49 522 256 1038 459 1740 1060 Fragments arranged by size: 1740 1060 1038 522 459 256 49 AfeI AGC'GCT Cuts at: 0 317 5124 Size: 317 4807 AflII C'TTAA_G Cuts at: 0 111 1561 1663 4149 5124 Size: 111 1450 102 2486 975 Fragments arranged by size: 2486 1450 975 111 102 AgeI A'CCGG_T Cuts at: 0 3149 5124 Size: 3149 1975 AleI CACnn'nnGTG Cuts at: 0 4818 5124 Size: 4818 306 AlwNI CAG_nnn'CTG Cuts at: 0 2173 5071 5124 Size: 2173 2898 53 Fragments arranged by size: 2898 2173 53 ApoI r'AATT_y Cuts at: 0 755 915 1828 2156 2233 3246 3297 3550 Size: 755 160 913 328 77 1013 51 253 Cuts at: 3550 3561 3846 4431 4479 4580 5124 Size: 11 285 585 48 101 544 Fragments arranged by size: 1013 913 755 585 544 328 285 253 160 101 77 51 48 11 AseI AT'TA_AT Cuts at: 0 505 896 4193 4862 5124 Size: 505 391 3297 669 262 Fragments arranged by size: 3297 669 505 391 262 AvaI C'yCGr_G Cuts at: 0 92 5124 Size: 92 5032 BamHI G'GATC_C Cuts at: 0 923 5124 Size: 923 4201 BanI G'GyrC_C Cuts at: 0 3861 5124 Size: 3861 1263 BanII G_rGCy'C Cuts at: 0 2921 3410 3631 3700 5124 Size: 2921 489 221 69 1424 Fragments arranged by size: 2921 1424 489 221 69 BbsI GAAGACnn'nnnn_ Cuts at: 0 830 1000 1465 1731 1927 2342 3515 4393 Size: 830 170 465 266 196 415 1173 878 Cuts at: 4393 4529 5124 Size: 136 595 Fragments arranged by size: 1173 878 830 595 465 415 266 196 170 136 BbvCI CC'TCA_GC Cuts at: 0 1112 5124 Size: 1112 4012 BciVI GTATCCnnnnn_n' Cuts at: 0 1560 4937 5124 Size: 1560 3377 187 Fragments arranged by size: 3377 1560 187 BclI T'GATC_A Cuts at: 0 1248 5124 Size: 1248 3876 BfrBI ATG'CAT Cuts at: 0 560 4023 4159 5055 5124 Size: 560 3463 136 896 69 Fragments arranged by size: 3463 896 560 136 69 BglII A'GATC_T Cuts at: 0 1872 5124 Size: 1872 3252 BlpI GC'TnA_GC Cuts at: 0 4752 5124 Size: 4752 372 BmgBI CAC'GTC Cuts at: 0 2625 5124 Size: 2625 2499 BmrI ACTGGGnnnn_n' Cuts at: 0 238 2976 3404 4910 5124 Size: 238 2738 428 1506 214 Fragments arranged by size: 2738 1506 428 238 214 BmtI G_CTAG'C Cuts at: 0 4760 5124 Size: 4760 364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 139 5124 Size: 139 4985 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 334 3851 4043 4436 5124 Size: 324 10 3517 192 393 688 Fragments arranged by size: 3517 688 393 324 192 10 Bpu10I CC'TnA_GC Cuts at: 0 1112 1460 5124 Size: 1112 348 3664 Fragments arranged by size: 3664 1112 348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3684 4727 5124 Size: 3684 1043 397 Fragments arranged by size: 3684 1043 397 BsaI GGTCTCn'nnnn_ Cuts at: 0 2821 5124 Size: 2821 2303 BsaAI yAC'GTr Cuts at: 0 3646 5124 Size: 3646 1478 BsaBI GATnn'nnATC Cuts at: 0 1529 3422 5124 Size: 1529 1893 1702 Fragments arranged by size: 1893 1702 1529 BsaHI Gr'CG_yC Cuts at: 0 3811 5124 Size: 3811 1313 BsaWI w'CCGG_w Cuts at: 0 2561 3149 5124 Size: 2561 588 1975 Fragments arranged by size: 2561 1975 588 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4409 4439 5124 Size: 4409 30 685 Fragments arranged by size: 4409 685 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 1045 5124 Size: 82 963 4079 Fragments arranged by size: 4079 963 82 BseYI C'CCAG_C Cuts at: 0 1747 2270 4597 5124 Size: 1747 523 2327 527 Fragments arranged by size: 2327 1747 527 523 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1430 4971 5124 Size: 1430 3541 153 Fragments arranged by size: 3541 1430 153 BsiEI CG_ry'CG Cuts at: 0 11 15 21 5124 Size: 11 4 6 5103 Fragments arranged by size: 5103 11 6 4 BsiHKAI G_wGCw'C Cuts at: 0 3631 3700 3900 5124 Size: 3631 69 200 1224 Fragments arranged by size: 3631 1224 200 69 BsmI GAATG_Cn' Cuts at: 0 474 4025 5124 Size: 474 3551 1099 Fragments arranged by size: 3551 1099 474 Bsp1286I G_dGCh'C Cuts at: 0 2921 3410 3631 3700 3900 5124 Size: 2921 489 221 69 200 1224 Fragments arranged by size: 2921 1224 489 221 200 69 BspHI T'CATG_A Cuts at: 0 4139 4893 5124 Size: 4139 754 231 Fragments arranged by size: 4139 754 231 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 877 2639 4124 5124 Size: 877 1762 1485 1000 Fragments arranged by size: 1762 1485 1000 877 BsrBI CCG'CTC Cuts at: 0 23 5124 Size: 23 5101 BsrDI GCAATG_nn' Cuts at: 0 560 5124 Size: 560 4564 BsrFI r'CCGG_y Cuts at: 0 3149 5124 Size: 3149 1975 BsrGI T'GTAC_A Cuts at: 0 3456 5037 5124 Size: 3456 1581 87 Fragments arranged by size: 3456 1581 87 BssSI C'ACGA_G Cuts at: 0 3710 5124 Size: 3710 1414 BstAPI GCAn_nnn'nTGC Cuts at: 0 562 5124 Size: 562 4562 BstXI CCAn_nnnn'nTGG Cuts at: 0 855 2011 5124 Size: 855 1156 3113 Fragments arranged by size: 3113 1156 855 BstYI r'GATC_y Cuts at: 0 84 923 1059 1144 1773 1872 5124 Size: 84 839 136 85 629 99 3252 Fragments arranged by size: 3252 839 629 136 99 85 84 BstZ17I GTA'TAC Cuts at: 0 4828 5124 Size: 4828 296 BtgI C'CryG_G Cuts at: 0 15 4232 5124 Size: 15 4217 892 Fragments arranged by size: 4217 892 15 BtsI GCAGTG_nn' Cuts at: 0 1338 2043 3342 4734 4983 5124 Size: 1338 705 1299 1392 249 141 Fragments arranged by size: 1392 1338 1299 705 249 141 ClaI AT'CG_AT Cuts at: 0 647 5124 Size: 647 4477 DraI TTT'AAA Cuts at: 0 1427 5124 Size: 1427 3697 DrdI GACnn_nn'nnGTC Cuts at: 0 547 3816 5003 5124 Size: 547 3269 1187 121 Fragments arranged by size: 3269 1187 547 121 EaeI y'GGCC_r Cuts at: 0 12 18 134 210 1669 3001 5124 Size: 12 6 116 76 1459 1332 2123 Fragments arranged by size: 2123 1459 1332 116 76 12 6 EagI C'GGCC_G Cuts at: 0 12 18 5124 Size: 12 6 5106 Fragments arranged by size: 5106 12 6 EarI CTCTTCn'nnn_ Cuts at: 0 1841 3888 5124 Size: 1841 2047 1236 Fragments arranged by size: 2047 1841 1236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 66 5124 Size: 66 5058 EcoICRI GAG'CTC Cuts at: 0 3629 3698 5124 Size: 3629 69 1426 Fragments arranged by size: 3629 1426 69 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 324 334 571 827 1865 2324 3851 Size: 49 275 10 237 256 1038 459 1527 Cuts at: 3851 4043 4064 4436 5124 Size: 192 21 372 688 Fragments arranged by size: 1527 1038 688 459 372 275 256 237 192 49 21 10 EcoO109I rG'GnC_Cy Cuts at: 0 675 1456 2897 5124 Size: 675 781 1441 2227 Fragments arranged by size: 2227 1441 781 675 EcoRI G'AATT_C Cuts at: 0 2233 4479 5124 Size: 2233 2246 645 Fragments arranged by size: 2246 2233 645 EcoRV GAT'ATC Cuts at: 0 249 325 1380 2316 3189 5124 Size: 249 76 1055 936 873 1935 Fragments arranged by size: 1935 1055 936 873 249 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1645 2794 5124 Size: 1645 1149 2330 Fragments arranged by size: 2330 1645 1149 HaeII r_GCGC'y Cuts at: 0 319 5124 Size: 319 4805 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 314 346 915 947 1240 1256 1272 1288 Size: 314 32 569 32 293 16 16 16 Cuts at: 1288 2970 3002 3687 3719 4723 4755 5124 Size: 1682 32 685 32 1004 32 369 Fragments arranged by size: 1682 1004 685 569 369 314 293 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 0 1969 2520 3083 5124 Size: 1969 551 563 2041 Fragments arranged by size: 2041 1969 563 551 HindIII A'AGCT_T Cuts at: 0 193 4046 5124 Size: 193 3853 1078 Fragments arranged by size: 3853 1078 193 MfeI C'AATT_G Cuts at: 0 1502 2479 5124 Size: 1502 977 2645 Fragments arranged by size: 2645 1502 977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 495 1063 2575 2666 4987 5124 Size: 495 568 1512 91 2321 137 Fragments arranged by size: 2321 1512 568 495 137 91 MscI TGG'CCA Cuts at: 0 136 212 1671 3003 5124 Size: 136 76 1459 1332 2121 Fragments arranged by size: 2121 1459 1332 136 76 MslI CAynn'nnrTG Cuts at: 0 2009 2365 2645 3101 3431 4093 4493 4547 Size: 2009 356 280 456 330 662 400 54 Cuts at: 4547 4818 5124 Size: 271 306 Fragments arranged by size: 2009 662 456 400 356 330 306 280 271 54 MspA1I CmG'CkG Cuts at: 0 17 62 81 805 1111 5124 Size: 17 45 19 724 306 4013 Fragments arranged by size: 4013 724 306 45 19 17 NcoI C'CATG_G Cuts at: 0 4232 5124 Size: 4232 892 NdeI CA'TA_TG Cuts at: 0 1295 3635 4251 4948 5124 Size: 1295 2340 616 697 176 Fragments arranged by size: 2340 1295 697 616 176 NheI G'CTAG_C Cuts at: 0 4756 5124 Size: 4756 368 NotI GC'GGCC_GC Cuts at: 0 18 5124 Size: 18 5106 NsiI A_TGCA'T Cuts at: 0 562 4025 4161 5057 5124 Size: 562 3463 136 896 67 Fragments arranged by size: 3463 896 562 136 67 NspI r_CATG'y Cuts at: 0 560 2333 5124 Size: 560 1773 2791 Fragments arranged by size: 2791 1773 560 PacI TTA_AT'TAA Cuts at: 0 1589 5124 Size: 1589 3535 PflMI CCAn_nnn'nTGG Cuts at: 0 132 2685 3532 5124 Size: 132 2553 847 1592 Fragments arranged by size: 2553 1592 847 132 PpuMI rG'GwC_Cy Cuts at: 0 1456 2897 5124 Size: 1456 1441 2227 Fragments arranged by size: 2227 1456 1441 PstI C_TGCA'G Cuts at: 0 2169 2185 5124 Size: 2169 16 2939 Fragments arranged by size: 2939 2169 16 PvuI CG_AT'CG Cuts at: 0 11 5124 Size: 11 5113 PvuII CAG'CTG Cuts at: 0 805 1111 5124 Size: 805 306 4013 Fragments arranged by size: 4013 805 306 SacI G_AGCT'C Cuts at: 0 3631 3700 5124 Size: 3631 69 1424 Fragments arranged by size: 3631 1424 69 SacII CC_GC'GG Cuts at: 0 18 5124 Size: 18 5106 SapI GCTCTTCn'nnn_ Cuts at: 0 3888 5124 Size: 3888 1236 ScaI AGT'ACT Cuts at: 0 944 3768 3780 5124 Size: 944 2824 12 1344 Fragments arranged by size: 2824 1344 944 12 SfcI C'TryA_G Cuts at: 0 783 2118 2165 2181 2827 4006 4534 5099 Size: 783 1335 47 16 646 1179 528 565 Cuts at: 5099 5124 Size: 25 Fragments arranged by size: 1335 1179 783 646 565 528 47 25 16 SmlI C'TyrA_G Cuts at: 0 111 1561 1663 3699 4149 4742 5124 Size: 111 1450 102 2036 450 593 382 Fragments arranged by size: 2036 1450 593 450 382 111 102 SnaBI TAC'GTA Cuts at: 0 3646 5124 Size: 3646 1478 SpeI A'CTAG_T Cuts at: 0 2655 3169 5124 Size: 2655 514 1955 Fragments arranged by size: 2655 1955 514 SphI G_CATG'C Cuts at: 0 560 5124 Size: 560 4564 SspI AAT'ATT Cuts at: 0 2700 5124 Size: 2700 2424 StyI C'CwwG_G Cuts at: 0 1385 1787 2388 2786 4232 5124 Size: 1385 402 601 398 1446 892 Fragments arranged by size: 1446 1385 892 601 402 398 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3236 3459 5124 Size: 3236 223 1665 Fragments arranged by size: 3236 1665 223 TatI w'GTAC_w Cuts at: 0 942 2658 2691 2910 3172 3456 3520 3766 Size: 942 1716 33 219 262 284 64 246 Cuts at: 3766 3778 4516 5037 5124 Size: 12 738 521 87 Fragments arranged by size: 1716 942 738 521 284 262 246 219 87 64 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1296 1328 5124 Size: 1296 32 3796 Fragments arranged by size: 3796 1296 32 Tth111I GACn'n_nGTC Cuts at: 0 791 5124 Size: 791 4333 XbaI T'CTAG_A Cuts at: 0 3091 3970 5124 Size: 3091 879 1154 Fragments arranged by size: 3091 1154 879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1773 4655 5124 Size: 1773 2882 469 Fragments arranged by size: 2882 1773 469 XmnI GAAnn'nnTTC Cuts at: 0 1832 1995 4694 5027 5124 Size: 1832 163 2699 333 97 Fragments arranged by size: 2699 1832 333 163 97 Enzymes that do cut: AccI AcuI AfeI AflII AgeI AleI AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I BtgI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PflMI PpuMI PstI PvuI PvuII SacI SacII SapI ScaI SfcI SmlI SnaBI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BglI Bme1580I BsiWI BsmBI BspEI BssHII BstBI BstEII Bsu36I BtgZI CspCI DraIII EcoNI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NruI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI RsrII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SrfI StuI SwaI XhoI XmaI ZraI