(Circular) (Six-base) MAPSORT of: pFN21AE9257.seq Check: 1935 from: 1 to: 7282 pFN21AE9257 7282 bp With 182 enzymes: SgfI * December 13, 2010 19:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3798 5092 278 Size: 53 83 186 936 2262 1294 2468 Fragments arranged by size: 2468 2262 1294 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4625 1937 Size: 2688 4594 Acc65I G'GTAC_C Cuts at: 4446 4603 4446 Size: 157 7125 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3425 3548 3719 4041 4500 5345 6729 6997 Size: 1793 123 171 322 459 845 1384 268 Cuts at: 6997 1632 Size: 1917 Fragments arranged by size: 1917 1793 1384 845 459 322 268 171 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7085 AflIII A'CryG_T Cuts at: 1203 3534 6181 7158 1203 Size: 2331 2647 977 1327 Fragments arranged by size: 2647 2331 1327 977 AgeI A'CCGG_T Cuts at: 6113 6113 Size: 7282 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 2531 4208 4373 1166 Size: 1365 1677 165 4075 Fragments arranged by size: 4075 1677 1365 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3167 1184 Size: 1983 5299 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2223 2255 3500 3532 2223 Size: 32 1245 32 5973 Fragments arranged by size: 5973 1245 32 32 AlwNI CAG_nnn'CTG Cuts at: 2488 3682 5025 6597 2488 Size: 1194 1343 1572 3173 Fragments arranged by size: 3173 1572 1343 1194 ApaLI G'TGCA_C Cuts at: 2920 3388 5339 6495 2920 Size: 468 1951 1156 3707 Fragments arranged by size: 3707 1951 1156 468 ApoI r'AATT_y Cuts at: 1493 3191 4591 4817 4969 7078 1493 Size: 1698 1400 226 152 2109 1697 Fragments arranged by size: 2109 1698 1697 1400 226 152 AseI AT'TA_AT Cuts at: 160 1032 5836 160 Size: 872 4804 1606 Fragments arranged by size: 4804 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2203 3436 4103 4607 1870 Size: 73 68 192 1233 667 504 4545 Fragments arranged by size: 4545 1233 667 504 192 73 68 BamHI G'GATC_C Cuts at: 4612 4979 4612 Size: 367 6915 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 3522 4134 4446 4603 Size: 325 217 627 1331 404 612 312 157 Cuts at: 4603 6058 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1331 627 612 404 325 312 217 157 BanII G_rGCy'C Cuts at: 1391 3513 3951 4601 1391 Size: 2122 438 650 4072 Fragments arranged by size: 4072 2122 650 438 BbeI G_GCGC'C Cuts at: 3122 3122 Size: 7282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5473 5507 1903 Size: 34 3536 34 3678 Fragments arranged by size: 3678 3536 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5174 6384 5174 Size: 1210 6072 BclI T'GATC_A Cuts at: 1548 3096 1548 Size: 1548 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3127 4439 5893 Size: 107 122 71 631 605 1455 1312 1454 Cuts at: 5893 136 Size: 1525 Fragments arranged by size: 1525 1455 1454 1312 631 605 122 107 71 BglII A'GATC_T Cuts at: 2259 3432 7277 2259 Size: 1173 3845 2264 Fragments arranged by size: 3845 2264 1173 BlpI GC'TnA_GC Cuts at: 4699 4699 Size: 7282 Bme1580I G_kGCm'C Cuts at: 1790 2204 2924 3392 5343 6499 1790 Size: 414 720 468 1951 1156 2573 Fragments arranged by size: 2573 1951 1156 720 468 414 BmgBI CAC'GTC Cuts at: 1318 2494 3226 4303 1318 Size: 1176 732 1077 4297 Fragments arranged by size: 4297 1176 1077 732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2926 3240 5013 Size: 416 53 83 399 24 1509 314 1773 Cuts at: 5013 5971 442 Size: 958 1753 Fragments arranged by size: 1773 1753 1509 958 416 399 314 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 3047 5942 1239 Size: 1130 678 2895 2579 Fragments arranged by size: 2895 2579 1130 678 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5407 6272 6570 5407 Size: 865 298 6119 Fragments arranged by size: 6119 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4509 5945 882 Size: 617 3010 1436 2219 Fragments arranged by size: 3010 2219 1436 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 4375 5073 493 Size: 711 2333 838 698 2702 Fragments arranged by size: 2702 2333 838 711 698 BsaBI GATnn'nnATC Cuts at: 7276 7276 Size: 7282 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3119 3303 3581 3795 Size: 53 83 186 936 1586 184 278 214 Cuts at: 3795 3861 5089 5471 6106 275 Size: 66 1228 382 635 1451 Fragments arranged by size: 1586 1451 1228 936 635 382 278 214 186 184 83 66 53 BsaWI w'CCGG_w Cuts at: 5715 6113 6387 6534 6945 5715 Size: 398 274 147 411 6052 Fragments arranged by size: 6052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3500 3530 3603 3633 3780 Size: 30 165 30 2097 30 73 30 147 Cuts at: 3780 3810 1178 Size: 30 4650 Fragments arranged by size: 4650 2097 165 147 73 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2221 3941 4318 1186 Size: 1035 1720 377 4150 Fragments arranged by size: 4150 1720 1035 377 BseYI C'CCAG_C Cuts at: 2459 3378 3622 3715 4402 6485 6981 2459 Size: 919 244 93 687 2083 496 2760 Fragments arranged by size: 2760 2083 919 687 496 244 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3665 3680 3917 4138 4145 4366 1163 Size: 573 1929 15 237 221 7 221 4079 Fragments arranged by size: 4079 1929 573 237 221 221 15 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 4732 5493 6521 1540 Size: 275 186 1126 1605 761 1028 2301 Fragments arranged by size: 2301 1605 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2924 3392 3951 5343 5428 6499 2924 Size: 468 559 1392 85 1071 3707 Fragments arranged by size: 3707 1392 1071 559 468 85 BsmI GAATG_Cn' Cuts at: 4793 4886 4793 Size: 93 7189 BsmBI CGTCTCn'nnnn_ Cuts at: 2468 3435 2468 Size: 967 6315 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2204 2924 3392 3513 3951 4601 5343 Size: 399 414 720 468 121 438 650 742 Cuts at: 5343 5428 6499 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 650 468 438 414 399 121 85 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 2499 5171 6901 2499 Size: 2672 1730 2880 Fragments arranged by size: 2880 2672 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4027 4637 844 Size: 3183 610 3489 Fragments arranged by size: 3489 3183 610 BsrBI CCG'CTC Cuts at: 1394 1823 2904 4171 5169 1394 Size: 429 1081 1267 998 3507 Fragments arranged by size: 3507 1267 1081 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2857 3360 3834 5777 5951 65 Size: 1183 1609 503 474 1943 174 1396 Fragments arranged by size: 1943 1609 1396 1183 503 474 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 3757 3906 5926 6113 1700 Size: 110 208 1739 149 2020 187 2869 Fragments arranged by size: 2869 2020 1739 208 187 149 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7282 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 4049 5342 6354 4049 Size: 1293 1012 4977 Fragments arranged by size: 4977 1293 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2039 3643 794 Size: 1245 1604 4433 Fragments arranged by size: 4433 1604 1245 BstBI TT'CG_AA Cuts at: 2139 6086 2139 Size: 3947 3335 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7282 BstXI CCAn_nnnn'nTGG Cuts at: 4496 4496 Size: 7282 BstYI r'GATC_y Cuts at: 1972 2259 3175 3432 4344 4612 4979 5364 5381 Size: 287 916 257 912 268 367 385 17 Cuts at: 5381 6833 6919 6931 7277 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 916 912 385 367 346 287 268 257 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2532 3843 3936 513 Size: 552 1467 1311 93 3859 Fragments arranged by size: 3859 1467 1311 552 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6543 Fragments arranged by size: 6543 683 56 BtsI GCAGTG_nn' Cuts at: 3644 4798 5592 5612 3644 Size: 1154 794 20 5314 Fragments arranged by size: 5314 1154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7247 DraI TTT'AAA Cuts at: 2251 2725 4586 2251 Size: 474 1861 4947 Fragments arranged by size: 4947 1861 474 DraIII CAC_nnn'GTG Cuts at: 3639 4429 4456 3639 Size: 790 27 6465 Fragments arranged by size: 6465 790 27 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6289 809 Size: 507 4973 1802 Fragments arranged by size: 4973 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3630 3930 4059 Size: 54 1425 50 275 18 1800 300 129 Cuts at: 4059 4530 4729 5618 7247 8 Size: 471 199 889 1629 43 Fragments arranged by size: 1800 1629 1425 889 471 300 275 199 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4729 1537 Size: 275 2917 4090 Fragments arranged by size: 4090 2917 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2825 3129 4124 4154 5212 1353 Size: 1472 304 995 30 1058 3423 Fragments arranged by size: 3423 1472 1058 995 304 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3512 5869 6243 6389 387 Size: 1064 138 1923 2357 374 146 1280 Fragments arranged by size: 2357 1923 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3949 3949 Size: 7282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 3047 3425 3548 3719 4041 4500 Size: 393 737 678 378 123 171 322 459 Cuts at: 4500 5345 5942 6729 6997 1239 Size: 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 737 678 597 459 393 378 322 268 171 123 EcoO109I rG'GnC_Cy Cuts at: 2100 2485 2100 Size: 385 6897 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 EcoRV GAT'ATC Cuts at: 2185 2185 Size: 7282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4305 4305 Size: 7282 FspI TGC'GCA Cuts at: 4565 5788 4565 Size: 1223 6059 HaeII r_GCGC'y Cuts at: 1678 3122 3385 4409 6429 1678 Size: 1444 263 1024 2020 2531 Fragments arranged by size: 2531 2020 1444 1024 263 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3780 3812 4001 4033 5923 Size: 32 135 32 2208 32 189 32 1890 Cuts at: 5923 5955 5997 6029 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2208 1890 189 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2215 4626 4872 669 Size: 988 281 277 2411 246 3079 Fragments arranged by size: 3079 2411 988 281 277 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7282 HpaI GTT'AAC Cuts at: 2215 4872 2215 Size: 2657 4625 KasI G'GCGC_C Cuts at: 3118 3118 Size: 7282 KpnI G_GTAC'C Cuts at: 4450 4607 4450 Size: 157 7125 MfeI C'AATT_G Cuts at: 4881 4881 Size: 7282 MluI A'CGCG_T Cuts at: 7158 7158 Size: 7282 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2974 3711 6396 6580 1305 Size: 1669 737 2685 184 2007 Fragments arranged by size: 2685 2007 1669 737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3632 4061 4532 10 Size: 54 1425 343 1800 429 471 2760 Fragments arranged by size: 2760 1800 1425 471 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2531 4121 4208 4373 4473 5240 Size: 648 1146 219 1590 87 165 100 767 Cuts at: 5240 5599 5758 518 Size: 359 159 2042 Fragments arranged by size: 2042 1590 1146 767 648 359 219 165 159 100 87 MspA1I CmG'CkG Cuts at: 1606 2890 3553 4360 4698 5375 6523 6768 1606 Size: 1284 663 807 338 677 1148 245 2120 Fragments arranged by size: 2120 1284 1148 807 677 663 338 245 NaeI GCC'GGC Cuts at: 2020 2020 Size: 7282 NarI GG'CG_CC Cuts at: 3119 3119 Size: 7282 NcoI C'CATG_G Cuts at: 513 1065 3936 513 Size: 552 2871 3859 Fragments arranged by size: 3859 2871 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7282 NgoMIV G'CCGG_C Cuts at: 2018 2018 Size: 7282 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7282 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 3316 3454 4640 6185 1132 Size: 97 2087 138 1186 1545 2229 Fragments arranged by size: 2229 2087 1545 1186 138 97 PasI CC'CwG_GG Cuts at: 1348 3745 1348 Size: 2397 4885 PciI A'CATG_T Cuts at: 6181 6181 Size: 7282 PflMI CCAn_nnn'nTGG Cuts at: 1108 2403 2488 4456 4528 1108 Size: 1295 85 1968 72 3862 Fragments arranged by size: 3862 1968 1295 85 72 PfoI T'CCnGG_A Cuts at: 2105 2105 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5351 5383 6864 6896 3500 Size: 32 1819 32 1481 32 3886 Fragments arranged by size: 3886 1819 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2100 2100 Size: 7282 PshAI GACnn'nnGTC Cuts at: 1613 3293 1613 Size: 1680 5602 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PstI C_TGCA'G Cuts at: 830 2895 3246 3552 4344 4634 830 Size: 2065 351 306 792 290 3478 Fragments arranged by size: 3478 2065 792 351 306 290 PvuI CG_AT'CG Cuts at: 2001 3127 2001 Size: 1126 6156 PvuII CAG'CTG Cuts at: 3553 4360 3553 Size: 807 6475 SacI G_AGCT'C Cuts at: 3951 3951 Size: 7282 SalI G'TCGA_C Cuts at: 1936 4624 1936 Size: 2688 4594 SapI GCTCTTCn'nnn_ Cuts at: 4154 4154 Size: 7282 SbfI CC_TGCA'GG Cuts at: 4634 4634 Size: 7282 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 4425 4452 4425 Size: 27 7255 SfcI C'TryA_G Cuts at: 826 1045 2891 3242 3548 4340 4630 5765 6446 Size: 219 1846 351 306 792 290 1135 681 Cuts at: 6446 6637 826 Size: 191 1471 Fragments arranged by size: 1846 1471 1135 792 681 351 306 290 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4439 4439 Size: 7282 SfoI GGC'GCC Cuts at: 3120 3120 Size: 7282 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 3757 1700 Size: 2057 5225 SmaI CCC'GGG Cuts at: 1872 4105 4609 1872 Size: 2233 504 4545 Fragments arranged by size: 4545 2233 504 SmlI C'TyrA_G Cuts at: 820 1017 1943 3436 5386 6287 6549 820 Size: 197 926 1493 1950 901 262 1553 Fragments arranged by size: 1950 1553 1493 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5073 493 Size: 4580 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 3454 4640 1132 Size: 2322 1186 3774 Fragments arranged by size: 3774 2322 1186 SspI AAT'ATT Cuts at: 5 52 2653 5206 5 Size: 47 2601 2553 2081 Fragments arranged by size: 2601 2553 2081 47 StuI AGG'CCT Cuts at: 1517 4073 4288 1517 Size: 2556 215 4511 Fragments arranged by size: 4511 2556 215 StyI C'CwwG_G Cuts at: 513 1065 2059 3936 4533 4721 513 Size: 552 994 1877 597 188 3074 Fragments arranged by size: 3074 1877 994 597 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 4241 5309 5326 5479 5664 6109 Size: 1255 815 1846 1068 17 153 185 445 Cuts at: 6109 325 Size: 1498 Fragments arranged by size: 1846 1498 1255 1068 815 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 4572 5528 Size: 274 80 33 51 1443 30 2565 956 Cuts at: 5528 7240 96 Size: 1712 138 Fragments arranged by size: 2565 1712 1443 956 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2498 2530 2827 2859 3004 3036 3603 3635 7242 Size: 32 297 32 145 32 567 32 3607 Cuts at: 7242 7274 2498 Size: 32 2506 Fragments arranged by size: 3607 2506 567 297 145 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2495 3682 4013 1316 Size: 1179 1187 331 4585 Fragments arranged by size: 4585 1187 1179 331 XbaI T'CTAG_A Cuts at: 4618 4618 Size: 7282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3841 4450 3841 Size: 609 6673 XhoI C'TCGA_G Cuts at: 1943 3436 1943 Size: 1493 5789 XmaI C'CCGG_G Cuts at: 1870 4103 4607 1870 Size: 2233 504 4545 Fragments arranged by size: 4545 2233 504 XmnI GAAnn'nnTTC Cuts at: 3410 5411 3410 Size: 2001 5281 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3796 5090 276 Size: 53 83 186 936 2262 1294 2468 Fragments arranged by size: 2468 2262 1294 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SrfI SwaI