(Circular) (Six-base) MAPSORT of: pFN21AE4382.seq Check: 1869 from: 1 to: 5914 pFN21AE4382 5914 bp With 182 enzymes: SgfI * March 28, 2012 14:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2829 3724 278 Size: 53 83 186 936 1293 895 2468 Fragments arranged by size: 2468 1293 936 895 186 83 53 AccI GT'mk_AC Cuts at: 1937 3257 1937 Size: 1320 4594 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3129 3188 3977 5361 5629 1632 Size: 1497 59 789 1384 268 1917 Fragments arranged by size: 1917 1497 1384 789 268 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 2224 4643 2224 Size: 2419 3495 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5914 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2441 2473 2441 Size: 32 5882 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3160 3223 3449 3601 5710 1493 Size: 1667 63 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 3239 1870 Size: 73 1296 4545 Fragments arranged by size: 4545 1296 73 BamHI G'GATC_C Cuts at: 2416 3244 3611 2416 Size: 828 367 4719 Fragments arranged by size: 4719 828 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 4690 618 Size: 325 217 627 1448 1455 1842 Fragments arranged by size: 1842 1455 1448 627 325 217 BanII G_rGCy'C Cuts at: 1391 3233 1391 Size: 1842 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2772 3117 3176 928 Size: 971 873 345 59 3666 Fragments arranged by size: 3666 971 873 345 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4105 4139 1903 Size: 34 2168 34 3678 Fragments arranged by size: 3678 2168 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 2206 1548 Size: 658 5256 BfrBI ATG'CAT Cuts at: 2259 2259 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 2198 3331 2198 Size: 1133 4781 Bme1580I G_kGCm'C Cuts at: 1790 3975 5131 1790 Size: 2185 1156 2573 Fragments arranged by size: 2573 2185 1156 BmgBI CAC'GTC Cuts at: 1318 2243 1318 Size: 925 4989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3645 4603 442 Size: 416 53 83 399 24 2228 958 1753 Fragments arranged by size: 2228 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 3162 4574 1239 Size: 1625 298 1412 2579 Fragments arranged by size: 2579 1625 1412 298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4039 4904 5202 4039 Size: 865 298 4751 Fragments arranged by size: 4751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2506 3028 4577 882 Size: 617 1007 522 1549 2219 Fragments arranged by size: 2219 1549 1007 617 522 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2826 3003 3721 4103 Size: 53 83 186 936 1293 177 718 382 Cuts at: 4103 4738 275 Size: 635 1451 Fragments arranged by size: 1451 1293 936 718 635 382 186 177 83 53 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3046 1186 Size: 1860 4054 BseYI C'CCAG_C Cuts at: 2727 2833 5117 5613 2727 Size: 106 2284 496 3028 Fragments arranged by size: 3028 2284 496 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 1163 Size: 573 1078 4263 Fragments arranged by size: 4263 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2930 3975 4060 5131 2930 Size: 1045 85 1071 3713 Fragments arranged by size: 3713 1071 1045 85 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2930 3233 3975 4060 5131 1391 Size: 399 1140 303 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 742 399 303 85 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 844 Size: 2425 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3801 1394 Size: 429 1978 3507 Fragments arranged by size: 3507 1978 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2571 4409 4583 65 Size: 1183 879 444 1838 174 1396 Fragments arranged by size: 1838 1396 1183 879 444 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4558 4745 1700 Size: 110 2748 187 2869 Fragments arranged by size: 2869 2748 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2416 3244 3611 3996 4013 5465 5551 5563 Size: 444 828 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 444 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3029 3029 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 2004 2265 513 Size: 552 939 261 4162 Fragments arranged by size: 4162 939 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 2797 3430 4224 4244 2797 Size: 633 794 20 4467 Fragments arranged by size: 4467 794 633 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 3361 4250 Size: 54 1425 50 275 18 331 1200 889 Cuts at: 4250 5879 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1200 889 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3844 1353 Size: 2491 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2864 3129 3162 3188 3977 4574 5361 Size: 393 1232 265 33 26 789 597 787 Cuts at: 5361 5629 1239 Size: 268 1524 Fragments arranged by size: 1524 1232 789 787 597 393 268 265 33 26 EcoNI CCTnn'n_nnAGG Cuts at: 3203 3203 Size: 5914 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FspI TGC'GCA Cuts at: 4420 4420 Size: 5914 HaeII r_GCGC'y Cuts at: 1678 2236 2603 5061 1678 Size: 558 367 2458 2531 Fragments arranged by size: 2531 2458 558 367 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 3025 3057 4555 Size: 32 135 32 638 32 783 32 1498 Cuts at: 4555 4587 4629 4661 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1498 783 638 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 2994 748 Size: 2246 3668 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 5028 5212 1305 Size: 1281 2442 184 2007 Fragments arranged by size: 2442 2007 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 10 Size: 54 1425 343 331 3761 Fragments arranged by size: 3761 1425 343 331 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2264 2275 2295 2661 3872 4231 Size: 648 889 209 11 20 366 1211 359 Cuts at: 4231 4390 518 Size: 159 2042 Fragments arranged by size: 2042 1211 889 648 366 359 209 159 20 11 MspA1I CmG'CkG Cuts at: 1606 2525 3330 4007 5155 5400 1606 Size: 919 805 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 805 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2265 513 Size: 552 939 261 4162 Fragments arranged by size: 4162 939 552 261 NdeI CA'TA_TG Cuts at: 387 2272 387 Size: 1885 4029 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NsiI A_TGCA'T Cuts at: 2261 2261 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2197 2867 3272 4817 1132 Size: 97 968 670 405 1545 2229 Fragments arranged by size: 2229 1545 968 670 405 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PfoI T'CCnGG_A Cuts at: 2027 2880 2027 Size: 853 5061 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 2112 3484 2112 Size: 1372 4542 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2741 2773 2741 Size: 32 5882 PstI C_TGCA'G Cuts at: 830 2483 3266 830 Size: 1653 783 3478 Fragments arranged by size: 3478 1653 783 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2525 2525 Size: 5914 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2479 3262 4397 5078 5269 826 Size: 219 1434 783 1135 681 191 1471 Fragments arranged by size: 1471 1434 1135 783 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 4919 5181 820 Size: 197 926 2075 901 262 1553 Fragments arranged by size: 2075 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 2323 3838 5 Size: 47 2271 1515 2081 Fragments arranged by size: 2271 2081 1515 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2004 2265 3353 513 Size: 552 939 261 1088 3074 Fragments arranged by size: 3074 1088 939 552 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3941 3958 4111 4296 4741 325 Size: 1255 2361 17 153 185 445 1498 Fragments arranged by size: 2361 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3060 3182 4160 Size: 274 80 33 51 1443 1083 122 978 Cuts at: 4160 5872 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1083 978 274 138 122 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 2390 1316 Size: 1074 4840 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2849 2849 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2827 3722 276 Size: 53 83 186 936 1293 895 2468 Fragments arranged by size: 2468 1293 936 895 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI