################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AfaI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AvaI AviII AvrII BanI BbeI BbrPI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PstI PvuI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 1073 AACGTT AA^CGTT AhdI 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 439 ATTAAT AT^TAAT AsnI 439 ATTAAT AT^TAAT AspEI 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 851 GGATCC G^GATCC BanII 869 GAGCTC G[GA]GC[CT]^C BbvCI 984 GCTGAGG CCTCAGC(-5/-2) BcgI 756 CGACGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1405 GCCCAACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 874 CTCCAG CTGGAG(16/14) BsgI 764 GTGCAG GTGCAG(16/14) BsmAI 1245 GTCTC GTCTC(1/5) Bst1107I 348 GTATAC GTA^TAC BstYI 851 GGATCC [GA]^GATC[CT] BstZ17I 348 GTATAC GTA^TAC Cac8I 750 GCATGC GC[ACGT]^[ACGT]GC DraI 496 TTTAAA TTT^AAA DraII 1113 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 729 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1113 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 770 CCATGG C^C[AT][AT]GG MfeI 419 CAATTG C^AATTG MflI 851 GGATCC [GA]^GATC[CT] MspA1I 560 CAGCTG C[AC]G^C[GT]G MunI 419 CAATTG C^AATTG NcoI 770 CCATGG C^CATGG NspI 750 GCATGC [GA]CATG^[CT] PflMI 1130 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1113 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1128 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 439 ATTAAT AT^TAAT Psp1406I 1073 AACGTT AA^CGTT PvuII 560 CAGCTG CAG^CTG SacI 869 GAGCTC GAGCT^C SfaNI 786 GATGC GCATC(5/9) SfcI 587 CTACAG C^T[GA][CT]AG SmlI 706 CTCAAG C^T[CT][GA]AG SphI 750 GCATGC GCATG^C StyI 770 CCATGG C^C[AT][AT]GG Tsp45I 401 GTGAC ^GT[GC]AC Van91I 1130 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 851 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 3 GTCTAC GT^[AC][GT]AC 348 GTATAC AcsI 519 AAATTT [GA]^AATT[CT] 1491 AAATTC AlwI 852 GATCC GGATC(4/5) 851 GGATC ApoI 519 AAATTT [GA]^AATT[CT] 1491 AAATTC Asp700I 726 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1579 GAAGCAATTC BciVI 994 GGATAC GTATCC(6/5) 1102 GGATAC BfaI 97 CTAG C^TAG 1154 CTAG BspHI 863 TCATGA T^CATGA 1563 TCATGA BstF5I 1196 CATCC GGATG(2/0) 54 GGATG DpnII 852 GATC ^GATC 1556 GATC DsaI 140 CCGTGG C^C[GA][CT]GG 770 CCATGG FauI 689 CCCGC CCCGC(4/6) 1614 GCGGG FokI 1196 CATCC GGATG(9/13) 54 GGATG HgaI 601 GACGC GACGC(5/10) 978 GACGC MaeI 97 CTAG C^TAG 1154 CTAG MaeIII 323 GTAAC ^GT[ACGT]AC 401 GTGAC MboI 852 GATC ^GATC 1556 GATC MslI 571 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 717 CACTGTGGTG NdeII 852 GATC ^GATC 1556 GATC RcaI 863 TCATGA T^CATGA 1563 TCATGA SapI 301 GAAGAGC GCTCTTC(1/4) 1157 GCTCTTC Sau3AI 852 GATC ^GATC 1556 GATC TaqI 47 TCGA T^CGA 824 TCGA TfiI 40 GAATC G^A[AT]TC 1569 GAATC TthHB8I 47 TCGA T^CGA 824 TCGA XmnI 726 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1579 GAAGCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AspHI AvaII BalI BbsI BbvI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrDI BssKI BstNI Cfr13I DdeI EaeI EarI EcoRII Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeII MboII MluNI MnlI MscI MseI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI Tru9I TseI Tsp509I TspRI