(Circular) (Six-base) MAPSORT of: pFN21AE1254.seq Check: 5669 from: 1 to: 6346 pFN21AE1254 6346 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2009 2354 3689 1937 Size: 72 345 1335 4594 Fragments arranged by size: 4594 1335 345 72 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 3079 4473 4846 1563 Size: 1516 1394 373 3063 Fragments arranged by size: 3063 1516 1394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 2724 2990 4409 5793 6061 1632 Size: 482 610 266 1419 1384 268 1917 Fragments arranged by size: 1917 1419 1384 610 482 268 266 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 5245 6222 1203 Size: 4042 977 1327 Fragments arranged by size: 4042 1327 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 2739 5075 2739 Size: 2336 4010 AleI CACnn'nnGTG Cuts at: 1166 2580 2726 1166 Size: 1414 146 4786 Fragments arranged by size: 4786 1414 146 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6346 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 2543 Size: 32 6314 AlwNI CAG_nnn'CTG Cuts at: 4089 5661 4089 Size: 1572 4774 ApaLI G'TGCA_C Cuts at: 4403 5559 4403 Size: 1156 5190 ApoI r'AATT_y Cuts at: 1493 2524 3496 3655 3881 4033 6142 1493 Size: 1031 972 159 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 972 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 2445 4900 160 Size: 872 1413 2455 1606 Fragments arranged by size: 2455 1606 1413 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 3671 1870 Size: 73 1728 4545 Fragments arranged by size: 4545 1728 73 BamHI G'GATC_C Cuts at: 2856 3676 4043 2856 Size: 820 367 5159 Fragments arranged by size: 5159 820 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3667 5122 618 Size: 325 217 627 1880 1455 1842 Fragments arranged by size: 1880 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2878 3665 1391 Size: 1487 787 4072 Fragments arranged by size: 4072 1487 787 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2124 2292 2406 2738 3537 928 Size: 971 225 168 114 332 799 3737 Fragments arranged by size: 3737 971 799 332 225 168 114 BbvCI CC'TCA_GC Cuts at: 2990 2990 Size: 6346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2750 2784 4537 4571 1903 Size: 34 813 34 1753 34 3678 Fragments arranged by size: 3678 1753 813 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2993 3101 4238 5448 2993 Size: 108 1137 1210 3891 Fragments arranged by size: 3891 1210 1137 108 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3416 4957 136 Size: 107 122 71 631 605 1744 1541 1525 Fragments arranged by size: 1744 1541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3763 3763 Size: 6346 Bme1580I G_kGCm'C Cuts at: 1790 3412 4407 5563 1790 Size: 1622 995 1156 2573 Fragments arranged by size: 2573 1622 1156 995 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4077 5035 442 Size: 416 53 83 399 24 2660 958 1753 Fragments arranged by size: 2660 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3261 3582 3261 Size: 321 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 5006 1239 Size: 1625 2142 2579 Fragments arranged by size: 2579 2142 1625 Bpu10I CC'TnA_GC Cuts at: 2153 2990 2153 Size: 837 5509 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2696 4471 5336 5634 2696 Size: 1775 865 298 3408 Fragments arranged by size: 3408 1775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4137 493 Size: 711 2933 2702 Fragments arranged by size: 2933 2702 711 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4153 4535 5170 275 Size: 53 83 186 936 2620 382 635 1451 Fragments arranged by size: 2620 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4779 5177 5451 5598 6009 4779 Size: 398 274 147 411 5116 Fragments arranged by size: 5116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2360 2390 3253 3283 1178 Size: 30 165 30 957 30 863 30 4241 Fragments arranged by size: 4241 957 863 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2102 2375 2378 2381 2384 2387 2405 Size: 871 45 273 3 3 3 3 18 Cuts at: 2405 3250 3258 3261 3264 3273 3276 3279 3282 Size: 845 8 3 3 9 3 3 3 Cuts at: 3282 3318 3321 3324 3333 1186 Size: 36 3 3 9 4199 Fragments arranged by size: 4199 871 845 273 45 36 18 9 9 8 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2260 2563 2995 5549 6045 2260 Size: 303 432 2554 496 2561 Fragments arranged by size: 2561 2554 496 432 303 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2790 1163 Size: 573 1054 4719 Fragments arranged by size: 4719 1054 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3796 4557 5585 1540 Size: 275 186 1795 761 1028 2301 Fragments arranged by size: 2301 1795 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 2878 2898 4407 4492 5563 2811 Size: 67 20 1509 85 1071 3594 Fragments arranged by size: 3594 1509 1071 85 67 20 BsmI GAATG_Cn' Cuts at: 3857 3950 3857 Size: 93 6253 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2811 2878 2898 3412 3665 4407 4492 Size: 399 1021 67 20 514 253 742 85 Cuts at: 4492 5563 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1021 742 514 399 253 85 67 20 BspEI T'CCGG_A Cuts at: 6009 6009 Size: 6346 BspHI T'CATG_A Cuts at: 2868 3568 4235 5965 2868 Size: 700 667 1730 3249 Fragments arranged by size: 3249 1730 700 667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 844 Size: 2857 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4233 1394 Size: 429 2410 3507 Fragments arranged by size: 3507 2410 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2548 2635 2781 3378 4841 5015 65 Size: 1183 1300 87 146 597 1463 174 1396 Fragments arranged by size: 1463 1396 1300 1183 597 174 146 87 BsrFI r'CCGG_y Cuts at: 1700 1810 4990 5177 1700 Size: 110 3180 187 2869 Fragments arranged by size: 3180 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6346 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 4406 5418 4406 Size: 1012 5334 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6346 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6346 BstYI r'GATC_y Cuts at: 1972 2856 3676 4043 4428 4445 5897 5983 5995 Size: 884 820 367 385 17 1452 86 12 Cuts at: 5995 6341 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 884 820 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2355 2355 Size: 6346 BtgI C'CryG_G Cuts at: 513 1065 2145 2775 513 Size: 552 1080 630 4084 Fragments arranged by size: 4084 1080 630 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 3862 4656 4676 3862 Size: 794 20 5532 Fragments arranged by size: 5532 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 2503 3650 2503 Size: 1147 5199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5353 809 Size: 507 4037 1802 Fragments arranged by size: 4037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2745 2778 2862 Size: 54 1425 50 275 18 915 33 84 Cuts at: 2862 3258 3793 4682 6311 8 Size: 396 535 889 1629 43 Fragments arranged by size: 1629 1425 915 889 535 396 275 84 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2301 2382 2406 3169 4276 1353 Size: 667 281 81 24 763 1107 3423 Fragments arranged by size: 3423 1107 763 667 281 81 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2876 2876 Size: 6346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2724 2864 2990 4409 5006 5793 Size: 393 482 610 140 126 1419 597 787 Cuts at: 5793 6061 1239 Size: 268 1524 Fragments arranged by size: 1524 1419 787 610 597 482 393 268 140 126 EcoO109I rG'GnC_Cy Cuts at: 3119 3119 Size: 6346 EcoRI G'AATT_C Cuts at: 3655 4033 3655 Size: 378 5968 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 6346 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 5493 1678 Size: 3815 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 2725 2757 3226 Size: 32 135 32 788 32 333 32 469 Cuts at: 3226 3258 4987 5019 5061 5093 1373 Size: 32 1729 32 42 32 2626 Fragments arranged by size: 2626 1729 788 469 333 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3690 3936 669 Size: 988 281 1752 246 3079 Fragments arranged by size: 3079 1752 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6346 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 2424 3945 2424 Size: 1521 4825 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2859 5460 5644 1305 Size: 789 765 2601 184 2007 Fragments arranged by size: 2601 2007 789 765 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 2780 2864 3260 10 Size: 54 1425 343 915 33 84 396 3096 Fragments arranged by size: 3096 1425 915 396 343 84 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2580 2726 4304 4663 4822 518 Size: 648 1414 146 1578 359 159 2042 Fragments arranged by size: 2042 1578 1414 648 359 159 146 MspA1I CmG'CkG Cuts at: 1606 2567 3762 4439 5587 5832 1606 Size: 961 1195 677 1148 245 2120 Fragments arranged by size: 2120 1195 1148 961 677 245 NcoI C'CATG_G Cuts at: 513 1065 2775 513 Size: 552 1710 4084 Fragments arranged by size: 4084 1710 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 2759 3704 5249 1132 Size: 97 1530 945 1545 2229 Fragments arranged by size: 2229 1545 1530 945 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 3141 1108 Size: 2033 4313 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3119 3119 Size: 6346 PshAI GACnn'nnGTC Cuts at: 1613 3137 1613 Size: 1524 4822 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PstI C_TGCA'G Cuts at: 830 3698 830 Size: 2868 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 2567 2567 Size: 6346 SacI G_AGCT'C Cuts at: 2878 2878 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SapI GCTCTTCn'nnn_ Cuts at: 2301 3169 2301 Size: 868 5478 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SfcI C'TryA_G Cuts at: 826 1045 2592 3694 4829 5510 5701 826 Size: 219 1547 1102 1135 681 191 1471 Fragments arranged by size: 1547 1471 1135 1102 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 3673 1872 Size: 1801 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2711 4450 5351 5613 820 Size: 197 926 768 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 768 262 197 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 2759 3704 1132 Size: 1627 945 3774 Fragments arranged by size: 3774 1627 945 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2775 3785 513 Size: 552 1710 1010 3074 Fragments arranged by size: 3074 1710 1010 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4373 4390 4543 4728 5173 325 Size: 1255 2793 17 153 185 445 1498 Fragments arranged by size: 2793 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4592 6304 96 Size: 274 80 33 51 1443 2615 1712 138 Fragments arranged by size: 2615 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3253 3285 6306 6338 3253 Size: 32 3021 32 3261 Fragments arranged by size: 3261 3021 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 3671 1870 Size: 1801 4545 XmnI GAAnn'nnTTC Cuts at: 2735 3588 4475 2735 Size: 853 887 4606 Fragments arranged by size: 4606 887 853 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI