################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BanI BbeI BbrPI BcgI BciVI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AflII 892 CTTAAG C^TTAAG AflIII 628 ACACGT A^C[GA][CT]GT ApaI 1426 GGGCCC GGGCC^C AspI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 998 CTCGGG C^[CT]CG[GA]G BalI 48 TGGCCA TGG^CCA BamHI 600 GGATCC G^GATCC BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BclI 1217 TGATCA T^GATCA BfaI 283 CTAG C^TAG BfrI 892 CTTAAG C^TTAAG BsaAI 973 TACGTA [CT]AC^GT[GA] BsaBI 595 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1055 GAGACC GGTCTC(1/5) BseRI 1384 GAGGAG GAGGAG(10/8) BsoBI 998 CTCGGG C^[CT]CG[GA]G BspHI 119 TCATGA T^CATGA BsrDI 641 GCAATG GCAATG(2/0) CfoI 1551 GCGC GCG^C DraI 622 TTTAAA TTT^AAA DraIII 52 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 48 TGGCCA [CT]^GGCC[GA] EcoNI 864 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1217 TGATCA T^GATCA HaeII 1550 GGCGCT [GA]GCGC^[CT] HhaI 1551 GCGC GCG^C HinP1I 1551 GCGC G^CGC HincII 1186 GTTGAC GT[CT]^[GA]AC HindII 1186 GTTGAC GT[CT]^[GA]AC MaeI 283 CTAG C^TAG MamI 595 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 48 TGGCCA TGG^CCA MscI 48 TGGCCA TGG^CCA MslI 1365 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 674 CATATG CA^TATG NheI 282 GCTAGC G^CTAGC NspI 1319 GCATGT [GA]CATG^[CT] PflFI 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 51 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 445 GAGTC GAGTC(4/5) PpuMI 1194 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1196 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 416 CTGCAG CTGCA^G RcaI 119 TCATGA T^CATGA SfaNI 177 GATGC GCATC(5/9) SmlI 892 CTTAAG C^T[CT][GA]AG SnaBI 973 TACGTA TAC^GTA SspI 949 AATATT AAT^ATT TfiI 1270 GAATC G^A[AT]TC Tth111I 826 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 51 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 721 GTAGAC GT^[AC][GT]AC 1203 GTCTAC AcsI 451 AAATTC [GA]^AATT[CT] 619 GAATTT ApoI 451 AAATTC [GA]^AATT[CT] 619 GAATTT BanII 1174 GAGCCC G[GA]GC[CT]^C 1426 GGGCCC BcnI 297 CCGGG CC^[GC]GG 700 CCGGG BmyI 1174 GAGCCC G[AGT]GC[ACT]^C 1426 GGGCCC BsmI 653 GAATGC GAATGC(1/-1) 1507 GCATTC Bsp1286I 1174 GAGCCC G[AGT]GC[ACT]^C 1426 GGGCCC BsrI 1512 CCAGT ACTGG(1/-1) 1590 CCAGT BstXI 29 CCAACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 449 CCAAATTCTTGG BstYI 600 GGATCC [GA]^GATC[CT] 851 AGATCC DsaI 794 CCATGG C^C[GA][CT]GG 1178 CCATGG EcoRV 751 GATATC GAT^ATC 1019 GATATC FauI 1011 GCGGG CCCGC(4/6) 1107 GCGGG HindIII 889 AAGCTT A^AGCTT 1490 AAGCTT HinfI 445 GAGTC G^A[ACGT]TC 1270 GAATC MflI 600 GGATCC [GA]^GATC[CT] 851 AGATCC NciI 297 CCGGG CC^[GC]GG 700 CCGGG NcoI 794 CCATGG C^CATGG 1178 CCATGG SfcI 375 CTATAG C^T[GA][CT]AG 416 CTGCAG XhoII 600 GGATCC [GA]^GATC[CT] 851 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AvaII BbsI BbvI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrBI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI StyI Tru9I TseI Tsp45I Tsp509I TspRI