(Circular) (Six-base) MAPSORT of: pFN21AE1980.seq Check: 1789 from: 1 to: 6376 pFN21AE1980 6376 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3209 3719 1937 Size: 790 482 510 4594 Fragments arranged by size: 4594 790 510 482 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2095 3302 3558 4439 5823 6091 1632 Size: 463 1207 256 881 1384 268 1917 Fragments arranged by size: 1917 1384 1207 881 463 268 256 AflII C'TTAA_G Cuts at: 820 1017 2897 820 Size: 197 1880 4299 Fragments arranged by size: 4299 1880 197 AflIII A'CryG_T Cuts at: 1203 2633 5275 6252 1203 Size: 1430 2642 977 1327 Fragments arranged by size: 2642 1430 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 4119 5691 4119 Size: 1572 4804 ApaI G_GGCC'C Cuts at: 3435 3435 Size: 6376 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2456 2624 3685 3911 4063 6172 1493 Size: 963 168 1061 226 152 2109 1697 Fragments arranged by size: 2109 1697 1061 963 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3003 3701 1870 Size: 73 1060 698 4545 Fragments arranged by size: 4545 1060 698 73 BamHI G'GATC_C Cuts at: 2605 3706 4073 2605 Size: 1101 367 4908 Fragments arranged by size: 4908 1101 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3697 5152 618 Size: 325 217 627 1910 1455 1842 Fragments arranged by size: 1910 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3183 3435 3695 1391 Size: 1792 252 260 4072 Fragments arranged by size: 4072 1792 260 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 2214 2440 2970 3300 928 Size: 971 213 102 226 530 330 4004 Fragments arranged by size: 4004 971 530 330 226 213 102 BbvCI CC'TCA_GC Cuts at: 2403 2403 Size: 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4567 4601 1903 Size: 34 50 34 2546 34 3678 Fragments arranged by size: 3678 2546 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 3222 1548 Size: 1674 4702 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 3793 3793 Size: 6376 Bme1580I G_kGCm'C Cuts at: 1790 3435 4437 5593 1790 Size: 1645 1002 1156 2573 Fragments arranged by size: 2573 1645 1156 1002 BmgBI CAC'GTC Cuts at: 1318 2171 2636 1318 Size: 853 465 5058 Fragments arranged by size: 5058 853 465 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4107 5065 442 Size: 416 53 83 399 24 2690 958 1753 Fragments arranged by size: 2690 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2291 1056 Size: 1235 5141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5036 1239 Size: 3797 2579 Bpu10I CC'TnA_GC Cuts at: 2403 2403 Size: 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4501 5366 5664 4501 Size: 865 298 5213 Fragments arranged by size: 5213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3054 5039 882 Size: 617 1555 1985 2219 Fragments arranged by size: 2219 1985 1555 617 BsaAI yAC'GTr Cuts at: 493 1204 2980 4167 493 Size: 711 1776 1187 2702 Fragments arranged by size: 2702 1776 1187 711 BsaBI GATnn'nnATC Cuts at: 2604 6370 2604 Size: 3766 2610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2969 2999 1178 Size: 30 165 30 1566 30 4555 Fragments arranged by size: 4555 1566 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3404 1186 Size: 2218 4158 BseYI C'CCAG_C Cuts at: 2212 3248 3261 3307 3352 5579 6075 2212 Size: 1036 13 46 45 2227 496 2513 Fragments arranged by size: 2513 2227 1036 496 46 45 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2136 2202 1163 Size: 573 388 12 66 5337 Fragments arranged by size: 5337 573 388 66 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4437 4522 5593 4437 Size: 85 1071 5220 Fragments arranged by size: 5220 1071 85 BsmI GAATG_Cn' Cuts at: 2664 3512 3887 3980 2664 Size: 848 375 93 5060 Fragments arranged by size: 5060 848 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3183 3435 3695 4437 4522 5593 1391 Size: 399 1393 252 260 742 85 1071 2174 Fragments arranged by size: 2174 1393 1071 742 399 260 252 85 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 2124 4265 5995 2124 Size: 2141 1730 2505 Fragments arranged by size: 2505 2141 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3731 844 Size: 2887 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2686 3016 3112 4263 1394 Size: 429 863 330 96 1151 3507 Fragments arranged by size: 3507 1151 863 429 330 96 BsrDI GCAATG_nn' Cuts at: 65 1248 2653 4871 5045 65 Size: 1183 1405 2218 174 1396 Fragments arranged by size: 2218 1405 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5020 5207 1700 Size: 110 3210 187 2869 Fragments arranged by size: 3210 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6376 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2461 2041 Size: 420 5956 BstYI r'GATC_y Cuts at: 1972 2605 2856 3706 4073 4458 4475 5927 6013 Size: 633 251 850 367 385 17 1452 86 Cuts at: 6013 6025 6371 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 850 633 385 367 346 251 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2870 2940 3436 2870 Size: 70 496 5810 Fragments arranged by size: 5810 496 70 BtgI C'CryG_G Cuts at: 513 1065 2799 3183 513 Size: 552 1734 384 3706 Fragments arranged by size: 3706 1734 552 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 2397 3892 4686 4706 2397 Size: 1495 794 20 4067 Fragments arranged by size: 4067 1495 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 2629 3680 2629 Size: 1051 5325 DraIII CAC_nnn'GTG Cuts at: 2062 2062 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 3823 4712 Size: 54 1425 50 275 18 223 1770 889 Cuts at: 4712 6341 8 Size: 1629 43 Fragments arranged by size: 1770 1629 1425 889 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2553 2919 3123 3134 3620 4306 1353 Size: 1070 130 366 204 11 486 686 3423 Fragments arranged by size: 3423 1070 686 486 366 204 130 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2423 3559 4963 5337 5483 387 Size: 1064 138 834 1136 1404 374 146 1280 Fragments arranged by size: 1404 1280 1136 1064 834 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 3302 3558 4439 5036 5823 6091 Size: 393 463 1207 256 881 597 787 268 Cuts at: 6091 1239 Size: 1524 Fragments arranged by size: 1524 1207 881 787 597 463 393 268 256 EcoNI CCTnn'n_nnAGG Cuts at: 2873 2873 Size: 6376 EcoO109I rG'GnC_Cy Cuts at: 3200 3431 3432 3200 Size: 231 1 6144 Fragments arranged by size: 6144 231 1 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 EcoRV GAT'ATC Cuts at: 2758 3026 2758 Size: 268 6108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3451 3602 3451 Size: 151 6225 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 3559 5523 1678 Size: 1881 1964 2531 Fragments arranged by size: 2531 1964 1881 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2427 2459 5017 Size: 32 135 32 488 32 335 32 2558 Cuts at: 5017 5049 5091 5123 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2558 488 335 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3193 3720 3966 669 Size: 988 281 1255 527 246 3079 Fragments arranged by size: 3079 1255 988 527 281 246 HindIII A'AGCT_T Cuts at: 748 2894 3495 748 Size: 2146 601 3629 Fragments arranged by size: 3629 2146 601 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 3354 5490 5674 1305 Size: 753 1296 2136 184 2007 Fragments arranged by size: 2136 2007 1296 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 10 Size: 54 1425 343 223 4331 Fragments arranged by size: 4331 1425 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 3374 4334 4693 4852 518 Size: 648 2208 960 359 159 2042 Fragments arranged by size: 2208 2042 960 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2216 2420 3307 3792 4469 5617 5862 1606 Size: 610 204 887 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 610 485 245 204 NcoI C'CATG_G Cuts at: 513 1065 2799 3183 513 Size: 552 1734 384 3706 Fragments arranged by size: 3706 1734 552 384 NdeI CA'TA_TG Cuts at: 387 2680 387 Size: 2293 4083 NheI G'CTAG_C Cuts at: 1052 2287 1052 Size: 1235 5141 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3328 3734 5279 1132 Size: 97 2099 406 1545 2229 Fragments arranged by size: 2229 2099 1545 406 97 PasI CC'CwG_GG Cuts at: 1348 2133 1348 Size: 785 5591 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 1108 Size: 954 5422 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 6376 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3200 3200 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 3205 1613 Size: 1592 4784 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 3431 3431 Size: 6376 PstI C_TGCA'G Cuts at: 830 2425 3728 830 Size: 1595 1303 3478 Fragments arranged by size: 3478 1595 1303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2216 2420 3307 2216 Size: 204 887 5285 Fragments arranged by size: 5285 887 204 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2380 2421 3724 4859 5540 5731 826 Size: 219 1335 41 1303 1135 681 191 1471 Fragments arranged by size: 1471 1335 1303 1135 681 219 191 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2897 4480 5381 5643 820 Size: 197 926 954 1583 901 262 1553 Fragments arranged by size: 1583 1553 954 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2980 4167 493 Size: 2487 1187 2702 Fragments arranged by size: 2702 2487 1187 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 2956 4300 5 Size: 47 2904 1344 2081 Fragments arranged by size: 2904 2081 1344 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2186 2313 2799 3183 3213 3504 3815 Size: 552 1121 127 486 384 30 291 311 Cuts at: 3815 513 Size: 3074 Fragments arranged by size: 3074 1121 552 486 384 311 291 127 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 2498 3084 3132 4403 4420 4573 Size: 1255 873 45 586 48 1271 17 153 Cuts at: 4573 4758 5203 325 Size: 185 445 1498 Fragments arranged by size: 1498 1271 1255 873 586 445 185 153 48 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 4622 6334 Size: 274 80 33 51 1443 216 2429 1712 Cuts at: 6334 96 Size: 138 Fragments arranged by size: 2429 1712 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6336 6368 6336 Size: 32 6344 Tth111I GACn'n_nGTC Cuts at: 1316 2834 1316 Size: 1518 4858 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI