Restriction map for
pFN21AE1980
(Linear) (Six-base) MAPSORT of: KIEE1980.seq Check: 3726 from: 1 to: 1671
KIEE1980.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 722 1204 1671
Size: 722 482 467
Fragments arranged by size:
722 482 467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1297 1553 1671
Size: 90 1207 256 118
Fragments arranged by size:
1207 256 118 90
AflII C'TTAA_G
Cuts at: 0 892 1671
Size: 892 779
AflIII A'CryG_T
Cuts at: 0 628 1671
Size: 628 1043
ApaI G_GGCC'C
Cuts at: 0 1430 1671
Size: 1430 241
ApoI r'AATT_y
Cuts at: 0 451 619 1671
Size: 451 168 1052
Fragments arranged by size:
1052 451 168
AvaI C'yCGr_G
Cuts at: 0 998 1671
Size: 998 673
BamHI G'GATC_C
Cuts at: 0 600 1671
Size: 600 1071
BanII G_rGCy'C
Cuts at: 0 1178 1430 1671
Size: 1178 252 241
Fragments arranged by size:
1178 252 241
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 209 435 965 1295 1671
Size: 107 102 226 530 330 376
Fragments arranged by size:
530 376 330 226 107 102
BbvCI CC'TCA_GC
Cuts at: 0 398 1671
Size: 398 1273
BclI T'GATC_A
Cuts at: 0 1217 1671
Size: 1217 454
Bme1580I G_kGCm'C
Cuts at: 0 1430 1671
Size: 1430 241
BmgBI CAC'GTC
Cuts at: 0 166 631 1671
Size: 166 465 1040
Fragments arranged by size:
1040 465 166
BmtI G_CTAG'C
Cuts at: 0 286 1671
Size: 286 1385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1671
Size: 42 1629
Bpu10I CC'TnA_GC
Cuts at: 0 398 1671
Size: 398 1273
BsaI GGTCTCn'nnnn_
Cuts at: 0 1049 1671
Size: 1049 622
BsaAI yAC'GTr
Cuts at: 0 975 1671
Size: 975 696
BsaBI GATnn'nnATC
Cuts at: 0 599 1671
Size: 599 1072
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 964 994 1671
Size: 964 30 677
Fragments arranged by size:
964 677 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1399 1671
Size: 1399 272
BseYI C'CCAG_C
Cuts at: 0 207 1243 1256 1302 1347 1671
Size: 207 1036 13 46 45 324
Fragments arranged by size:
1036 324 207 46 45 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 131 197 1671
Size: 119 12 66 1474
Fragments arranged by size:
1474 119 66 12
BsmI GAATG_Cn'
Cuts at: 0 659 1507 1671
Size: 659 848 164
Fragments arranged by size:
848 659 164
Bsp1286I G_dGCh'C
Cuts at: 0 1178 1430 1671
Size: 1178 252 241
Fragments arranged by size:
1178 252 241
BspHI T'CATG_A
Cuts at: 0 119 1671
Size: 119 1552
BsrBI CCG'CTC
Cuts at: 0 681 1011 1107 1671
Size: 681 330 96 564
Fragments arranged by size:
681 564 330 96
BsrDI GCAATG_nn'
Cuts at: 0 648 1671
Size: 648 1023
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 456 1671
Size: 36 420 1215
Fragments arranged by size:
1215 420 36
BstYI r'GATC_y
Cuts at: 0 600 851 1671
Size: 600 251 820
Fragments arranged by size:
820 600 251
Bsu36I CC'TnA_GG
Cuts at: 0 865 935 1431 1671
Size: 865 70 496 240
Fragments arranged by size:
865 496 240 70
BtgI C'CryG_G
Cuts at: 0 794 1178 1671
Size: 794 384 493
Fragments arranged by size:
794 493 384
BtsI GCAGTG_nn'
Cuts at: 0 392 1671
Size: 392 1279
DraI TTT'AAA
Cuts at: 0 624 1671
Size: 624 1047
DraIII CAC_nnn'GTG
Cuts at: 0 57 1671
Size: 57 1614
EaeI y'GGCC_r
Cuts at: 0 48 1671
Size: 48 1623
EarI CTCTTCn'nnn_
Cuts at: 0 418 548 914 1118 1129 1615 1671
Size: 418 130 366 204 11 486 56
Fragments arranged by size:
486 418 366 204 130 56 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 1554 1671
Size: 418 1136 117
Fragments arranged by size:
1136 418 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1297 1553 1671
Size: 90 1207 256 118
Fragments arranged by size:
1207 256 118 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 868 1671
Size: 868 803
EcoO109I rG'GnC_Cy
Cuts at: 0 1195 1426 1427 1671
Size: 1195 231 1 244
Fragments arranged by size:
1195 244 231 1
EcoRV GAT'ATC
Cuts at: 0 753 1021 1671
Size: 753 268 650
Fragments arranged by size:
753 650 268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1446 1597 1671
Size: 1446 151 74
Fragments arranged by size:
1446 151 74
HaeII r_GCGC'y
Cuts at: 0 1554 1671
Size: 1554 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 422 454 1671
Size: 55 32 335 32 1217
Fragments arranged by size:
1217 335 55 32 32
HincII GTy'rAC
Cuts at: 0 1188 1671
Size: 1188 483
HindIII A'AGCT_T
Cuts at: 0 889 1490 1671
Size: 889 601 181
Fragments arranged by size:
889 601 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1349 1671
Size: 53 1296 322
Fragments arranged by size:
1296 322 53
MscI TGG'CCA
Cuts at: 0 50 1671
Size: 50 1621
MslI CAynn'nnrTG
Cuts at: 0 1369 1671
Size: 1369 302
MspA1I CmG'CkG
Cuts at: 0 211 415 1302 1671
Size: 211 204 887 369
Fragments arranged by size:
887 369 211 204
NcoI C'CATG_G
Cuts at: 0 794 1178 1671
Size: 794 384 493
Fragments arranged by size:
794 493 384
NdeI CA'TA_TG
Cuts at: 0 675 1671
Size: 675 996
NheI G'CTAG_C
Cuts at: 0 282 1671
Size: 282 1389
NspI r_CATG'y
Cuts at: 0 1323 1671
Size: 1323 348
PasI CC'CwG_GG
Cuts at: 0 128 1671
Size: 128 1543
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 1671
Size: 57 1614
PfoI T'CCnGG_A
Cuts at: 0 440 1671
Size: 440 1231
PpuMI rG'GwC_Cy
Cuts at: 0 1195 1671
Size: 1195 476
PshAI GACnn'nnGTC
Cuts at: 0 1200 1671
Size: 1200 471
PspOMI G'GGCC_C
Cuts at: 0 1426 1671
Size: 1426 245
PstI C_TGCA'G
Cuts at: 0 420 1671
Size: 420 1251
PvuII CAG'CTG
Cuts at: 0 211 415 1302 1671
Size: 211 204 887 369
Fragments arranged by size:
887 369 211 204
SfcI C'TryA_G
Cuts at: 0 375 416 1671
Size: 375 41 1255
Fragments arranged by size:
1255 375 41
SmlI C'TyrA_G
Cuts at: 0 892 1671
Size: 892 779
SnaBI TAC'GTA
Cuts at: 0 975 1671
Size: 975 696
SspI AAT'ATT
Cuts at: 0 951 1671
Size: 951 720
StyI C'CwwG_G
Cuts at: 0 181 308 794 1178 1208 1499 1671
Size: 181 127 486 384 30 291 172
Fragments arranged by size:
486 384 291 181 172 127 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 448 493 1079 1127 1671
Size: 448 45 586 48 544
Fragments arranged by size:
586 544 448 48 45
TatI w'GTAC_w
Cuts at: 0 188 1671
Size: 188 1483
Tth111I GACn'n_nGTC
Cuts at: 0 829 1671
Size: 829 842
Enzymes that do cut:
AccI AcuI AflII AflIII ApaI ApoI AvaI BamHI
BanII BbsI BbvCI BclI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BspHI BsrBI BsrDI BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NdeI NheI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SfcI SmlI SnaBI SspI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BcgI BciVI BfrBI BglI BglII
BlpI BmrI BpmI BpuEI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DrdI
EagI EcoICRI EcoRI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SphI SrfI StuI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI