################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI KpnI KspI MaeII MamI MfeI MluI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 923 TCCGGA T^CCGGA AflIII 1157 ACATGT A^C[GA][CT]GT AspI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1319 GGCGCC GGCGC^C BbvCI 2326 CCTCAGC CCTCAGC(-5/-2) BcgI 390 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1864 GCCAAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 923 TCCGGA [AT]^CCGG[AT] BseAI 923 TCCGGA T^CCGGA BsgI 1530 CTGCAC GTGCAG(16/14) BsmI 2136 GAATGC GAATGC(1/-1) BspEI 923 TCCGGA T^CCGGA BspLU11I 1157 ACATGT A^CATGT BspMI 692 ACCTGC ACCTGC(4/8) EcoRI 731 GAATTC G^AATTC HincII 985 GTTAAC GT[CT]^[GA]AC HindII 985 GTTAAC GT[CT]^[GA]AC HindIII 2000 AAGCTT A^AGCTT HpaI 985 GTTAAC GTT^AAC KasI 1319 GGCGCC G^GCGCC MroI 923 TCCGGA T^CCGGA NarI 1319 GGCGCC GG^CGCC PaeR7I 290 CTCGAG C^TCGAG PflFI 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 266 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1356 AGTACT AGT^ACT SexAI 1520 ACCTGGT A^CC[AT]GGT SfoI 1319 GGCGCC GGC^GCC SmaI 500 CCCGGG CCC^GGG SpeI 1023 ACTAGT A^CTAGT StuI 1919 AGGCCT AGG^CCT Tth111I 1486 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 290 CTCGAG C^TCGAG XmaCI 500 CCCGGG C^CCGGG XmaI 500 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1570 GTAGAC GT^[AC][GT]AC 1807 GTAGAC AspHI 137 GTGCTC G[AT]GC[AT]^C 1448 GTGCTC BalI 809 TGGCCA TGG^CCA 1626 TGGCCA BbsI 340 GAAGAC GAAGAC(2/6) 1413 GAAGAC BlpI 751 GCTCAGC GC^T[ACGT]AGC 1243 GCTGAGC Bpu1102I 751 GCTCAGC GC^T[ACGT]AGC 1243 GCTGAGC BpuAI 340 GAAGAC GAAGAC(2/6) 1413 GAAGAC BsiHKAI 137 GTGCTC G[AT]GC[AT]^C 1448 GTGCTC BsrBI 1144 GAGCGG CCGCTC(-3/-3) 1246 GAGCGG BsrDI 215 CATTGC GCAATG(2/0) 1728 CATTGC BsrFI 791 ACCGGC [GA]^CCGG[CT] 2169 ACCGGC CelII 751 GCTCAGC GC^T[ACGT]AGC 1243 GCTGAGC Cfr10I 791 ACCGGC [GA]^CCGG[CT] 2169 ACCGGC DrdI 189 GACAAATAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 570 GACCCCTGAGTC DsaI 369 CCACGG C^C[GA][CT]GG 1610 CCACGG EaeI 809 TGGCCA [CT]^GGCC[GA] 1626 TGGCCA MluNI 809 TGGCCA TGG^CCA 1626 TGGCCA MscI 809 TGGCCA TGG^CCA 1626 TGGCCA MseI 281 TTAA T^TAA 986 TTAA MslI 1212 CACGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1309 CACTATGATG PpuMI 264 GGGACCC [GA]G^G[AT]CC[CT] 374 GGGACCT TfiI 89 GATTC G^A[AT]TC 1036 GATTC Tru9I 281 TTAA T^TAA 986 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII