(Circular) (Six-base) MAPSORT of: pFN21AE3432.seq Check: 8278 from: 1 to: 7129 pFN21AE3432 7129 bp With 182 enzymes: SgfI * March 28, 2012 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4939 278 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3576 3813 4472 1937 Size: 1639 237 659 4594 Fragments arranged by size: 4594 1639 659 237 Acc65I G'GTAC_C Cuts at: 4450 4450 Size: 7129 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2373 5192 6576 6844 1632 Size: 741 2819 1384 268 1917 Fragments arranged by size: 2819 1917 1384 741 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 3162 6028 7005 1203 Size: 1959 2866 977 1327 Fragments arranged by size: 2866 1959 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 5858 5858 Size: 7129 AleI CACnn'nnGTG Cuts at: 1166 3221 1166 Size: 2055 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2088 1184 Size: 904 6225 AlwNI CAG_nnn'CTG Cuts at: 2318 3898 3940 4420 4872 6444 2318 Size: 1580 42 480 452 1572 3003 Fragments arranged by size: 3003 1580 1572 480 452 42 ApaLI G'TGCA_C Cuts at: 5186 6342 5186 Size: 1156 5973 ApoI r'AATT_y Cuts at: 1493 2119 2736 3749 3784 4438 4664 4816 6925 Size: 626 617 1013 35 654 226 152 2109 Cuts at: 6925 1493 Size: 1697 Fragments arranged by size: 2109 1697 1013 654 626 617 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 2295 2505 3068 4454 1870 Size: 73 352 210 563 1386 4545 Fragments arranged by size: 4545 1386 563 352 210 73 BamHI G'GATC_C Cuts at: 2523 2718 2998 3153 3406 4459 4826 2523 Size: 195 280 155 253 1053 367 4826 Fragments arranged by size: 4826 1053 367 280 253 195 155 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2908 3133 3324 3610 Size: 325 217 627 285 836 225 191 286 Cuts at: 3610 4450 5905 618 Size: 840 1455 1842 Fragments arranged by size: 1842 1455 840 836 627 325 286 285 225 217 191 BanII G_rGCy'C Cuts at: 1391 2177 2393 2758 4448 1391 Size: 786 216 365 1690 4072 Fragments arranged by size: 4072 1690 786 365 216 BbeI G_GCGC'C Cuts at: 3328 3328 Size: 7129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2352 3425 928 Size: 971 453 1073 4632 Fragments arranged by size: 4632 1073 971 453 BbvCI CC'TCA_GC Cuts at: 4332 4332 Size: 7129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2384 2418 5320 5354 1903 Size: 34 447 34 2902 34 3678 Fragments arranged by size: 3678 2902 447 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5021 6231 5021 Size: 1210 5919 BclI T'GATC_A Cuts at: 1548 1548 Size: 7129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3875 5740 136 Size: 107 122 71 631 605 2203 1865 1525 Fragments arranged by size: 2203 1865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7124 7124 Size: 7129 BlpI GC'TnA_GC Cuts at: 2757 3249 4546 2757 Size: 492 1297 5340 Fragments arranged by size: 5340 1297 492 Bme1580I G_kGCm'C Cuts at: 1790 3054 3087 3175 3615 4160 5190 6346 1790 Size: 1264 33 88 440 545 1030 1156 2573 Fragments arranged by size: 2573 1264 1156 1030 545 440 88 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2269 3399 3669 Size: 416 53 83 399 24 852 1130 270 Cuts at: 3669 4086 4860 5818 442 Size: 417 774 958 1753 Fragments arranged by size: 1753 1130 958 852 774 417 416 399 270 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 7129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2676 3091 3198 3446 3997 5789 1239 Size: 1437 415 107 248 551 1792 2579 Fragments arranged by size: 2579 1792 1437 551 415 248 107 Bpu10I CC'TnA_GC Cuts at: 4332 4332 Size: 7129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2786 3242 5254 6119 6417 2786 Size: 456 2012 865 298 3498 Fragments arranged by size: 3498 2012 865 456 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2537 2781 2952 5792 882 Size: 617 1038 244 171 2840 2219 Fragments arranged by size: 2840 2219 1038 617 244 171 BsaAI yAC'GTr Cuts at: 493 1204 4920 493 Size: 711 3716 2702 Fragments arranged by size: 3716 2702 711 BsaBI GATnn'nnATC Cuts at: 7123 7123 Size: 7129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2953 3240 3325 4936 Size: 53 83 186 936 1420 287 85 1611 Cuts at: 4936 5318 5953 275 Size: 382 635 1451 Fragments arranged by size: 1611 1451 1420 936 635 382 287 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2928 5562 5960 6234 6381 6792 2928 Size: 2634 398 274 147 411 3265 Fragments arranged by size: 3265 2634 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4333 4363 1178 Size: 30 165 30 2930 30 3944 Fragments arranged by size: 3944 2930 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 2283 2351 2354 2465 2555 2570 1186 Size: 993 104 68 3 111 90 15 5745 Fragments arranged by size: 5745 993 111 104 90 68 15 3 BseYI C'CCAG_C Cuts at: 2311 2519 2663 3080 3201 3286 6332 6828 2311 Size: 208 144 417 121 85 3046 496 2612 Fragments arranged by size: 3046 2612 496 417 208 144 121 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3520 1163 Size: 573 1784 4772 Fragments arranged by size: 4772 1784 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4579 5340 6368 1540 Size: 275 186 2578 761 1028 2301 Fragments arranged by size: 2578 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2146 3457 5190 5275 6346 2146 Size: 1311 1733 85 1071 2929 Fragments arranged by size: 2929 1733 1311 1071 85 BsmI GAATG_Cn' Cuts at: 4147 4640 4733 4147 Size: 493 93 6543 Fragments arranged by size: 6543 493 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2177 2393 2758 3054 3087 3175 Size: 399 356 31 216 365 296 33 88 Cuts at: 3175 3457 3615 4160 4448 5190 5275 6346 1391 Size: 282 158 545 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 365 356 296 288 282 216 158 88 85 33 31 BspEI T'CCGG_A Cuts at: 2928 6792 2928 Size: 3864 3265 BspHI T'CATG_A Cuts at: 5018 6748 5018 Size: 1730 5399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 4484 844 Size: 1862 1778 3489 Fragments arranged by size: 3489 1862 1778 BsrBI CCG'CTC Cuts at: 1394 1823 3151 3253 5016 1394 Size: 429 1328 102 1763 3507 Fragments arranged by size: 3507 1763 1328 429 102 BsrDI GCAATG_nn' Cuts at: 65 1248 2219 3732 5624 5798 65 Size: 1183 971 1513 1892 174 1396 Fragments arranged by size: 1892 1513 1396 1183 971 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2796 4174 5773 5960 1700 Size: 110 986 1378 1599 187 2869 Fragments arranged by size: 2869 1599 1378 986 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 5189 6201 5189 Size: 1012 6117 BstAPI GCAn_nnn'nTGC Cuts at: 794 3091 3898 4270 794 Size: 2297 807 372 3653 Fragments arranged by size: 3653 2297 807 372 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstXI CCAn_nnnn'nTGG Cuts at: 2258 3196 3320 3343 2258 Size: 938 124 23 6044 Fragments arranged by size: 6044 938 124 23 BstYI r'GATC_y Cuts at: 1972 2523 2718 2809 2998 3153 3406 4459 4826 Size: 551 195 91 189 155 253 1053 367 Cuts at: 4826 5211 5228 6680 6766 6778 7124 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1053 551 385 367 346 253 195 189 155 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2208 3822 4133 4218 2208 Size: 1614 311 85 5119 Fragments arranged by size: 5119 1614 311 85 BtgI C'CryG_G Cuts at: 513 1065 2004 2374 3615 513 Size: 552 939 370 1241 4027 Fragments arranged by size: 4027 1241 939 552 370 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6390 Fragments arranged by size: 6390 683 56 BtsI GCAGTG_nn' Cuts at: 2329 3388 4645 5439 5459 2329 Size: 1059 1257 794 20 3999 Fragments arranged by size: 3999 1257 1059 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 4433 4433 Size: 7129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2200 2581 6136 809 Size: 507 884 381 3555 1802 Fragments arranged by size: 3555 1802 884 507 381 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 3631 4576 Size: 54 1425 50 275 18 984 817 945 Cuts at: 4576 5465 7094 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 984 945 889 817 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4576 1537 Size: 275 2764 4090 Fragments arranged by size: 4090 2764 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2328 2532 2556 2598 2870 3552 5059 1353 Size: 975 204 24 42 272 682 1507 3423 Fragments arranged by size: 3423 1507 975 682 272 204 42 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5716 6090 6236 387 Size: 1064 138 4127 374 146 1280 Fragments arranged by size: 4127 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2373 2676 3091 3198 3446 3997 5192 Size: 393 741 303 415 107 248 551 1195 Cuts at: 5192 5789 6576 6844 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1195 787 741 597 551 415 393 303 268 248 107 EcoNI CCTnn'n_nnAGG Cuts at: 2583 2966 3464 2583 Size: 383 498 6248 Fragments arranged by size: 6248 498 383 EcoO109I rG'GnC_Cy Cuts at: 2270 2380 2588 2617 4328 2270 Size: 110 208 29 1711 5071 Fragments arranged by size: 5071 1711 208 110 29 EcoRI G'AATT_C Cuts at: 2736 4438 4816 2736 Size: 1702 378 5049 Fragments arranged by size: 5049 1702 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3946 3946 Size: 7129 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 2311 3328 3667 6276 1678 Size: 633 1017 339 2609 2531 Fragments arranged by size: 2609 2531 1017 633 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 5770 5802 5844 Size: 32 135 32 851 32 3315 32 42 Cuts at: 5844 5876 1373 Size: 32 2626 Fragments arranged by size: 3315 2626 851 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2992 4473 4719 669 Size: 988 281 1054 1481 246 3079 Fragments arranged by size: 3079 1481 1054 988 281 246 HindIII A'AGCT_T Cuts at: 748 4005 748 Size: 3257 3872 HpaI GTT'AAC Cuts at: 2992 4719 2992 Size: 1727 5402 KasI G'GCGC_C Cuts at: 3324 3324 Size: 7129 KpnI G_GTAC'C Cuts at: 4454 4454 Size: 7129 MfeI C'AATT_G Cuts at: 4728 4728 Size: 7129 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 2460 6243 6427 1305 Size: 693 462 3783 184 2007 Fragments arranged by size: 3783 2007 693 462 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2816 3633 10 Size: 54 1425 343 984 817 3506 Fragments arranged by size: 3506 1425 984 817 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3221 3318 5087 5446 5605 518 Size: 648 2055 97 1769 359 159 2042 Fragments arranged by size: 2055 2042 1769 648 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2258 2761 3205 3898 4040 4417 4545 5222 Size: 652 503 444 693 142 377 128 677 Cuts at: 5222 6370 6615 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 693 677 652 503 444 377 245 142 128 NarI GG'CG_CC Cuts at: 3325 3325 Size: 7129 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5638 Fragments arranged by size: 5638 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 3094 3115 3166 4487 6032 1132 Size: 97 1865 21 51 1321 1545 2229 Fragments arranged by size: 2229 1865 1545 1321 97 51 21 PasI CC'CwG_GG Cuts at: 1348 3004 1348 Size: 1656 5473 PciI A'CATG_T Cuts at: 3162 6028 3162 Size: 2866 4263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2225 2318 3718 1108 Size: 1117 93 1400 4519 Fragments arranged by size: 4519 1400 1117 93 PfoI T'CCnGG_A Cuts at: 2721 3156 4020 2721 Size: 435 864 5830 Fragments arranged by size: 5830 864 435 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 2380 2270 Size: 110 7019 PshAI GACnn'nnGTC Cuts at: 1613 2275 1613 Size: 662 6467 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PspXI vC'TCGA_Gb Cuts at: 2295 2295 Size: 7129 PstI C_TGCA'G Cuts at: 830 2107 2625 3099 3383 3903 4481 830 Size: 1277 518 474 284 520 578 3478 Fragments arranged by size: 3478 1277 578 520 518 474 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 PvuII CAG'CTG Cuts at: 2258 3205 3898 4040 4417 2258 Size: 947 693 142 377 4970 Fragments arranged by size: 4970 947 693 377 142 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SanDI GG'GwC_CC Cuts at: 2270 2270 Size: 7129 SbfI CC_TGCA'GG Cuts at: 4481 4481 Size: 7129 ScaI AGT'ACT Cuts at: 3363 5377 7089 3363 Size: 2014 1712 3403 Fragments arranged by size: 3403 2014 1712 SexAI A'CCwGG_T Cuts at: 3525 3525 Size: 7129 SfcI C'TryA_G Cuts at: 826 1045 2103 2621 3095 3379 3899 4477 5612 Size: 219 1058 518 474 284 520 578 1135 Cuts at: 5612 6293 6484 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1058 681 578 520 518 474 284 219 191 SfoI GGC'GCC Cuts at: 3326 3326 Size: 7129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 2507 4456 1872 Size: 635 1949 4545 Fragments arranged by size: 4545 1949 635 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 2801 3257 5233 6134 6396 Size: 197 926 352 506 456 1976 901 262 Cuts at: 6396 820 Size: 1553 Fragments arranged by size: 1976 1553 926 901 506 456 352 262 197 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 3028 152 Size: 2876 4253 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 5053 5 Size: 47 5001 2081 Fragments arranged by size: 5001 2081 47 StuI AGG'CCT Cuts at: 1517 3926 1517 Size: 2409 4720 StyI C'CwwG_G Cuts at: 513 1065 2004 3129 3336 3402 3870 4152 4237 Size: 552 939 1125 207 66 468 282 85 Cuts at: 4237 4248 4323 4568 513 Size: 11 75 245 3074 Fragments arranged by size: 3074 1125 939 552 468 282 245 207 85 75 66 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 3372 3559 5156 5173 5326 5511 Size: 1255 981 811 187 1597 17 153 185 Cuts at: 5511 5956 325 Size: 445 1498 Fragments arranged by size: 1597 1498 1255 981 811 445 187 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3361 5375 7087 Size: 274 80 33 51 1443 1384 2014 1712 Cuts at: 7087 96 Size: 138 Fragments arranged by size: 2014 1712 1443 1384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3018 3050 4333 4365 7089 7121 3018 Size: 32 1283 32 2724 32 3026 Fragments arranged by size: 3026 2724 1283 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3494 1316 Size: 2178 4951 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 3719 3941 2167 Size: 1552 222 5355 Fragments arranged by size: 5355 1552 222 XhoI C'TCGA_G Cuts at: 1943 2295 1943 Size: 352 6777 XmaI C'CCGG_G Cuts at: 1870 2505 4454 1870 Size: 635 1949 4545 Fragments arranged by size: 4545 1949 635 XmnI GAAnn'nnTTC Cuts at: 5258 5258 Size: 7129 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4937 276 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI