(Linear) (Six-base) MAPSORT of: KIEE3432.seq Check: 2785 from: 1 to: 2424 KIEE3432.orf With 182 enzymes: SgfI * March 22, 2012 16:33 .. AccI GT'mk_AC Cuts at: 0 1571 1808 2424 Size: 1571 237 616 Fragments arranged by size: 1571 616 237 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 2424 Size: 368 2056 AflIII A'CryG_T Cuts at: 0 1157 2424 Size: 1157 1267 AleI CACnn'nnGTG Cuts at: 0 1216 2424 Size: 1216 1208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 83 2424 Size: 83 2341 AlwNI CAG_nnn'CTG Cuts at: 0 313 1893 1935 2415 2424 Size: 313 1580 42 480 9 Fragments arranged by size: 1580 480 313 42 9 ApoI r'AATT_y Cuts at: 0 114 731 1744 1779 2424 Size: 114 617 1013 35 645 Fragments arranged by size: 1013 645 617 114 35 AvaI C'yCGr_G Cuts at: 0 290 500 1063 2424 Size: 290 210 563 1361 Fragments arranged by size: 1361 563 290 210 BamHI G'GATC_C Cuts at: 0 518 713 993 1148 1401 2424 Size: 518 195 280 155 253 1023 Fragments arranged by size: 1023 518 280 253 195 155 BanI G'GyrC_C Cuts at: 0 67 903 1128 1319 1605 2424 Size: 67 836 225 191 286 819 Fragments arranged by size: 836 819 286 225 191 67 BanII G_rGCy'C Cuts at: 0 172 388 753 2424 Size: 172 216 365 1671 Fragments arranged by size: 1671 365 216 172 BbeI G_GCGC'C Cuts at: 0 1323 2424 Size: 1323 1101 BbsI GAAGACnn'nnnn_ Cuts at: 0 347 1420 2424 Size: 347 1073 1004 Fragments arranged by size: 1073 1004 347 BbvCI CC'TCA_GC Cuts at: 0 2327 2424 Size: 2327 97 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 379 413 2424 Size: 379 34 2011 Fragments arranged by size: 2011 379 34 BglI GCCn_nnn'nGGC Cuts at: 0 1870 2424 Size: 1870 554 BlpI GC'TnA_GC Cuts at: 0 752 1244 2424 Size: 752 492 1180 Fragments arranged by size: 1180 752 492 Bme1580I G_kGCm'C Cuts at: 0 1049 1082 1170 1610 2155 2424 Size: 1049 33 88 440 545 269 Fragments arranged by size: 1049 545 440 269 88 33 BmrI ACTGGGnnnn_n' Cuts at: 0 264 1394 1664 2081 2424 Size: 264 1130 270 417 343 Fragments arranged by size: 1130 417 343 270 264 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 360 2424 Size: 360 2064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 671 1086 1193 1441 1992 2424 Size: 671 415 107 248 551 432 Fragments arranged by size: 671 551 432 415 248 107 Bpu10I CC'TnA_GC Cuts at: 0 2327 2424 Size: 2327 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 1237 2424 Size: 781 456 1187 Fragments arranged by size: 1187 781 456 BsaI GGTCTCn'nnnn_ Cuts at: 0 532 776 947 2424 Size: 532 244 171 1477 Fragments arranged by size: 1477 532 244 171 BsaHI Gr'CG_yC Cuts at: 0 948 1235 1320 2424 Size: 948 287 85 1104 Fragments arranged by size: 1104 948 287 85 BsaWI w'CCGG_w Cuts at: 0 923 2424 Size: 923 1501 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2328 2358 2424 Size: 2328 30 66 Fragments arranged by size: 2328 66 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 174 278 346 349 460 550 565 2424 Size: 174 104 68 3 111 90 15 1859 Fragments arranged by size: 1859 174 111 104 90 68 15 3 BseYI C'CCAG_C Cuts at: 0 306 514 658 1075 1196 1281 2424 Size: 306 208 144 417 121 85 1143 Fragments arranged by size: 1143 417 306 208 144 121 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1515 2424 Size: 1515 909 BsiHKAI G_wGCw'C Cuts at: 0 141 1452 2424 Size: 141 1311 972 Fragments arranged by size: 1311 972 141 BsmI GAATG_Cn' Cuts at: 0 2142 2424 Size: 2142 282 Bsp1286I G_dGCh'C Cuts at: 0 141 172 388 753 1049 1082 1170 1452 Size: 141 31 216 365 296 33 88 282 Cuts at: 1452 1610 2155 2424 Size: 158 545 269 Fragments arranged by size: 545 365 296 282 269 216 158 141 88 33 31 BspEI T'CCGG_A Cuts at: 0 923 2424 Size: 923 1501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 701 2424 Size: 701 1723 BsrBI CCG'CTC Cuts at: 0 1146 1248 2424 Size: 1146 102 1176 Fragments arranged by size: 1176 1146 102 BsrDI GCAATG_nn' Cuts at: 0 214 1727 2424 Size: 214 1513 697 Fragments arranged by size: 1513 697 214 BsrFI r'CCGG_y Cuts at: 0 791 2169 2424 Size: 791 1378 255 Fragments arranged by size: 1378 791 255 BstAPI GCAn_nnn'nTGC Cuts at: 0 1086 1893 2265 2424 Size: 1086 807 372 159 Fragments arranged by size: 1086 807 372 159 BstXI CCAn_nnnn'nTGG Cuts at: 0 253 1191 1315 1338 2424 Size: 253 938 124 23 1086 Fragments arranged by size: 1086 938 253 124 23 BstYI r'GATC_y Cuts at: 0 518 713 804 993 1148 1401 2424 Size: 518 195 91 189 155 253 1023 Fragments arranged by size: 1023 518 253 195 189 155 91 Bsu36I CC'TnA_GG Cuts at: 0 203 1817 2128 2213 2424 Size: 203 1614 311 85 211 Fragments arranged by size: 1614 311 211 203 85 BtgI C'CryG_G Cuts at: 0 369 1610 2424 Size: 369 1241 814 Fragments arranged by size: 1241 814 369 BtsI GCAGTG_nn' Cuts at: 0 324 1383 2424 Size: 324 1059 1041 Fragments arranged by size: 1059 1041 324 DrdI GACnn_nn'nnGTC Cuts at: 0 195 576 2424 Size: 195 381 1848 Fragments arranged by size: 1848 381 195 EaeI y'GGCC_r Cuts at: 0 809 1626 2424 Size: 809 817 798 Fragments arranged by size: 817 809 798 EarI CTCTTCn'nnn_ Cuts at: 0 323 527 551 593 865 1547 2424 Size: 323 204 24 42 272 682 877 Fragments arranged by size: 877 682 323 272 204 42 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 671 1086 1193 1441 1992 2424 Size: 368 303 415 107 248 551 432 Fragments arranged by size: 551 432 415 368 303 248 107 EcoNI CCTnn'n_nnAGG Cuts at: 0 578 961 1459 2424 Size: 578 383 498 965 Fragments arranged by size: 965 578 498 383 EcoO109I rG'GnC_Cy Cuts at: 0 265 375 583 612 2323 2424 Size: 265 110 208 29 1711 101 Fragments arranged by size: 1711 265 208 110 101 29 EcoRI G'AATT_C Cuts at: 0 731 2424 Size: 731 1693 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1941 2424 Size: 1941 483 HaeII r_GCGC'y Cuts at: 0 306 1323 1662 2424 Size: 306 1017 339 762 Fragments arranged by size: 1017 762 339 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 418 450 2424 Size: 418 32 1974 Fragments arranged by size: 1974 418 32 HincII GTy'rAC Cuts at: 0 987 2424 Size: 987 1437 HindIII A'AGCT_T Cuts at: 0 2000 2424 Size: 2000 424 HpaI GTT'AAC Cuts at: 0 987 2424 Size: 987 1437 KasI G'GCGC_C Cuts at: 0 1319 2424 Size: 1319 1105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 455 2424 Size: 455 1969 MscI TGG'CCA Cuts at: 0 811 1628 2424 Size: 811 817 796 Fragments arranged by size: 817 811 796 MslI CAynn'nnrTG Cuts at: 0 1216 1313 2424 Size: 1216 97 1111 Fragments arranged by size: 1216 1111 97 MspA1I CmG'CkG Cuts at: 0 253 756 1200 1893 2035 2412 2424 Size: 253 503 444 693 142 377 12 Fragments arranged by size: 693 503 444 377 253 142 12 NarI GG'CG_CC Cuts at: 0 1320 2424 Size: 1320 1104 NspI r_CATG'y Cuts at: 0 1089 1110 1161 2424 Size: 1089 21 51 1263 Fragments arranged by size: 1263 1089 51 21 PasI CC'CwG_GG Cuts at: 0 999 2424 Size: 999 1425 PciI A'CATG_T Cuts at: 0 1157 2424 Size: 1157 1267 PflMI CCAn_nnn'nTGG Cuts at: 0 220 313 1713 2424 Size: 220 93 1400 711 Fragments arranged by size: 1400 711 220 93 PfoI T'CCnGG_A Cuts at: 0 716 1151 2015 2424 Size: 716 435 864 409 Fragments arranged by size: 864 716 435 409 PpuMI rG'GwC_Cy Cuts at: 0 265 375 2424 Size: 265 110 2049 Fragments arranged by size: 2049 265 110 PshAI GACnn'nnGTC Cuts at: 0 270 2424 Size: 270 2154 PspXI vC'TCGA_Gb Cuts at: 0 290 2424 Size: 290 2134 PstI C_TGCA'G Cuts at: 0 102 620 1094 1378 1898 2424 Size: 102 518 474 284 520 526 Fragments arranged by size: 526 520 518 474 284 102 PvuII CAG'CTG Cuts at: 0 253 1200 1893 2035 2412 2424 Size: 253 947 693 142 377 12 Fragments arranged by size: 947 693 377 253 142 12 SanDI GG'GwC_CC Cuts at: 0 265 2424 Size: 265 2159 ScaI AGT'ACT Cuts at: 0 1358 2424 Size: 1358 1066 SexAI A'CCwGG_T Cuts at: 0 1520 2424 Size: 1520 904 SfcI C'TryA_G Cuts at: 0 98 616 1090 1374 1894 2424 Size: 98 518 474 284 520 530 Fragments arranged by size: 530 520 518 474 284 98 SfoI GGC'GCC Cuts at: 0 1321 2424 Size: 1321 1103 SmaI CCC'GGG Cuts at: 0 502 2424 Size: 502 1922 SmlI C'TyrA_G Cuts at: 0 290 796 1252 2424 Size: 290 506 456 1172 Fragments arranged by size: 1172 506 456 290 SpeI A'CTAG_T Cuts at: 0 1023 2424 Size: 1023 1401 StuI AGG'CCT Cuts at: 0 1921 2424 Size: 1921 503 StyI C'CwwG_G Cuts at: 0 1124 1331 1397 1865 2147 2232 2243 2318 Size: 1124 207 66 468 282 85 11 75 Cuts at: 2318 2424 Size: 106 Fragments arranged by size: 1124 468 282 207 106 85 75 66 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 556 1367 1554 2424 Size: 556 811 187 870 Fragments arranged by size: 870 811 556 187 TatI w'GTAC_w Cuts at: 0 1356 2424 Size: 1356 1068 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1013 1045 2328 2360 2424 Size: 1013 32 1283 32 64 Fragments arranged by size: 1283 1013 64 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1489 2424 Size: 1489 935 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 162 1714 1936 2424 Size: 162 1552 222 488 Fragments arranged by size: 1552 488 222 162 XhoI C'TCGA_G Cuts at: 0 290 2424 Size: 290 2134 XmaI C'CCGG_G Cuts at: 0 500 2424 Size: 500 1924 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI MslI MspA1I NarI NspI PasI PciI PflMI PfoI PpuMI PshAI PspXI PstI PvuII SanDI ScaI SexAI SfcI SfoI SmaI SmlI SpeI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRV FseI FspI FspAI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI XmnI ZraI