################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 226 CGCG CG^CG AcsI 382 AAATTC [GA]^AATT[CT] AfaI 126 GTAC GT^AC AflIII 320 ACGTGT A^C[GA][CT]GT AlwI 282 GATCC GGATC(4/5) AocI 234 CCTGAGG CC^T[ACGT]AGG ApaI 308 GGGCCC GGGCC^C ApoI 382 AAATTC [GA]^AATT[CT] AvaI 311 CCCGGG C^[CT]CG[GA]G AvaII 96 GGACC G^G[AT]CC BbsI 136 GAAGAC GAAGAC(2/6) BglI 167 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 136 GAAGAC GAAGAC(2/6) BsiYI 171 CCTGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 171 CCTGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 377 GAGAC GTCTC(1/5) BsmFI 314 GGGAC GGGAC(10/14) BsoBI 311 CCCGGG C^[CT]CG[GA]G BsrI 128 ACTGG ACTGG(1/-1) BstF5I 213 CATCC GGATG(2/0) BstNI 171 CCTGG CC^[AT]GG BstUI 226 CGCG CG^CG Bsu36I 234 CCTGAGG CC^T[ACGT]AGG DdeI 235 CTGAG C^T[ACGT]AG Eco81I 234 CCTGAGG CC^T[ACGT]AGG EcoNI 171 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 171 CCTGG ^CC[AT]GG EcoT14I 200 CCAAGG C^C[AT][AT]GG FokI 213 CATCC GGATG(9/13) HapII 312 CCGG C^CGG HgaI 224 GACGC GACGC(5/10) HinfI 454 GAATC G^A[ACGT]TC HpaII 312 CCGG C^CGG MaeII 320 ACGT A^CGT MspI 312 CCGG C^CGG MvaI 171 CCTGG CC^[AT]GG MvnI 226 CGCG CG^CG NlaIII 402 CATG CATG^ RsaI 126 GTAC GT^AC ScaI 125 AGTACT AGT^ACT SmaI 311 CCCGGG CCC^GGG SmlI 145 CTCAAG C^T[CT][GA]AG StyI 200 CCAAGG C^C[AT][AT]GG TfiI 454 GAATC G^A[AT]TC Tsp45I 161 GTGAC ^GT[GC]AC Tsp509I 383 AATT ^AATT XmaCI 311 CCCGGG C^CCGGG XmaI 311 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BanI 247 GGCACC G^G[CT][GA]CC 445 GGCACC BanII 183 GGGCTC G[GA]GC[CT]^C 308 GGGCCC BcnI 311 CCCGG CC^[GC]GG 312 CCGGG BmyI 183 GGGCTC G[AGT]GC[ACT]^C 308 GGGCCC Bsp1286I 183 GGGCTC G[AGT]GC[ACT]^C 308 GGGCCC FauI 73 GCGGG CCCGC(4/6) 306 GCGGG HaeIII 204 GGCC GG^CC 309 GGCC HphI 300 GGTGA GGTGA(8/7) 327 TCACC MaeIII 80 GTAAC ^GT[ACGT]AC 161 GTGAC NciI 311 CCCGG CC^[GC]GG 312 CCGGG SfaNI 33 GATGC GCATC(5/9) 303 GATGC TaqI 293 TCGA T^CGA 332 TCGA TthHB8I 293 TCGA T^CGA 332 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI BbvI BsaJI BssKI Cac8I Cfr13I DpnII Fnu4HI ItaI MboI MboII MnlI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TseI