Restriction map for
pFN21AE9255
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BanI BbeI BbrPI
BclI BcnI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BstBI BstUI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
FbaI FseI FspI HaeII HapII
HgaI Hin1I HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 3579 ACATGT A^C[GA][CT]GT
AhdI 3118 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3218 CCTTAGG CC^T[ACGT]AGG
Asp700I 3530 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3118 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 50 GGACC G^G[AT]CC
BalI 2844 TGGCCA TGG^CCA
BamHI 1533 GGATCC G^GATCC
BbsI 3058 GAAGAC GAAGAC(2/6)
BcgI 1449 GCAGCTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2791 GTATCC GTATCC(6/5)
BglI 1246 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3058 GAAGAC GAAGAC(2/6)
BseRI 2266 GAGGAG GAGGAG(10/8)
BsmFI 1981 GGGAC GGGAC(10/14)
BspLU11I 3579 ACATGT A^CATGT
BsrDI 1855 GCAATG GCAATG(2/0)
BssSI 1457 CTCGTG CACGAG(-5/-1)
Bst1107I 881 GTATAC GTA^TAC
BstAPI 1186 GCAACTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2622 GGTGACC G^GT[ACGT]ACC
BstPI 2622 GGTGACC G^GT[ACGT]ACC
BstZ17I 881 GTATAC GTA^TAC
Bsu36I 3218 CCTTAGG CC^T[ACGT]AGG
EaeI 2844 TGGCCA [CT]^GGCC[GA]
Eam1105I 3118 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3218 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2622 GGTGACC G^GT[ACGT]ACC
FauI 2085 GCGGG CCCGC(4/6)
HincII 412 GTTAAC GT[CT]^[GA]AC
HindII 412 GTTAAC GT[CT]^[GA]AC
HindIII 784 AAGCTT A^AGCTT
HpaI 412 GTTAAC GTT^AAC
MluNI 2844 TGGCCA TGG^CCA
MscI 2844 TGGCCA TGG^CCA
NdeI 1484 CATATG CA^TATG
PshAI 51 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2482 GAGCTC GAGCT^C
XcmI 1537 CCAGAGAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3530 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 881 GTATAC GT^[AC][GT]AC
1660 GTAGAC
AciI 1151 CCGC CCGC(-3/-1)
2085 GCGG
AclI 1804 AACGTT AA^CGTT
3460 AACGTT
AcsI 1739 AAATTT [GA]^AATT[CT]
3041 AAATTC
ApoI 1739 AAATTT [GA]^AATT[CT]
3041 AAATTC
AseI 2821 ATTAAT AT^TAAT
3288 ATTAAT
AsnI 2821 ATTAAT AT^TAAT
3288 ATTAAT
BanII 2087 GGGCTC G[GA]GC[CT]^C
2482 GAGCTC
BbvCI 748 GCTGAGG CCTCAGC(-5/-2)
2575 GCTGAGG
BsgI 539 CTGCAC GTGCAG(16/14)
1124 CTGCAC
BspHI 291 TCATGA T^CATGA
834 TCATGA
CfoI 944 GCGC GCG^C
1443 GCGC
EcoNI 159 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3333 CCTTGATGAGG
EcoT22I 1 ATGCAT ATGCA^T
2417 ATGCAT
HhaI 944 GCGC GCG^C
1443 GCGC
HinP1I 944 GCGC G^CGC
1443 GCGC
MslI 1135 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3420 CATTGTGGTG
NlaIV 508 GGAACC GG[ACGT]^[ACGT]CC
1533 GGATCC
NsiI 1 ATGCAT ATGCA^T
2417 ATGCAT
PshBI 2821 ATTAAT AT^TAAT
3288 ATTAAT
Psp1406I 1804 AACGTT AA^CGTT
3460 AACGTT
RcaI 291 TCATGA T^CATGA
834 TCATGA
SapI 2479 GAAGAGC GCTCTTC(1/4)
2983 GAAGAGC
SphI 1077 GCATGC GCATG^C
1272 GCATGC
SspI 341 AATATT AAT^ATT
3091 AATATT
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
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AfaI AluI AlwI AlwNI AspHI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII