Restriction map for
pFN21AE9255
(Linear) (Six-base) MAPSORT of: KIEE9255.seq Check: 1470 from: 1 to: 3591
KIEE9255.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 3591
Size: 306 3285
AccI GT'mk_AC
Cuts at: 0 882 1661 3591
Size: 882 779 1930
Fragments arranged by size:
1930 882 779
AclI AA'CG_TT
Cuts at: 0 1805 3461 3591
Size: 1805 1656 130
Fragments arranged by size:
1805 1656 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 772 1130 1325 1831 2189 2792 2890
Size: 746 26 358 195 506 358 603 98
Cuts at: 2890 3002 3591
Size: 112 589
Fragments arranged by size:
746 603 589 506 358 358 195 112
98 26
AflIII A'CryG_T
Cuts at: 0 3579 3591
Size: 3579 12
AhdI GACnn_n'nnGTC
Cuts at: 0 3123 3591
Size: 3123 468
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 3591
Size: 1157 2434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 272 304 3591
Size: 272 32 3287
Fragments arranged by size:
3287 272 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2353 2980 3390 3591
Size: 2353 627 410 201
Fragments arranged by size:
2353 627 410 201
ApoI r'AATT_y
Cuts at: 0 1739 3041 3591
Size: 1739 1302 550
Fragments arranged by size:
1739 1302 550
AseI AT'TA_AT
Cuts at: 0 2822 3289 3591
Size: 2822 467 302
Fragments arranged by size:
2822 467 302
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 722 755 3591
Size: 722 33 2836
Fragments arranged by size:
2836 722 33
BamHI G'GATC_C
Cuts at: 0 1533 3591
Size: 1533 2058
BanII G_rGCy'C
Cuts at: 0 2091 2486 3591
Size: 2091 395 1105
Fragments arranged by size:
2091 1105 395
BbsI GAAGACnn'nnnn_
Cuts at: 0 3065 3591
Size: 3065 526
BbvCI CC'TCA_GC
Cuts at: 0 749 2576 3591
Size: 749 1827 1015
Fragments arranged by size:
1827 1015 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1438 1472 3591
Size: 1438 34 2119
Fragments arranged by size:
2119 1438 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2802 3591
Size: 2802 789
BfrBI ATG'CAT
Cuts at: 0 3 2419 3591
Size: 3 2416 1172
Fragments arranged by size:
2416 1172 3
BglI GCCn_nnn'nGGC
Cuts at: 0 1252 3591
Size: 1252 2339
BlpI GC'TnA_GC
Cuts at: 0 569 744 917 3591
Size: 569 175 173 2674
Fragments arranged by size:
2674 569 175 173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1660 1796 3591
Size: 1660 136 1795
Fragments arranged by size:
1795 1660 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 896 1655 2182 3223 3591
Size: 458 438 759 527 1041 368
Fragments arranged by size:
1041 759 527 458 438 368
Bpu10I CC'TnA_GC
Cuts at: 0 749 2576 3591
Size: 749 1827 1015
Fragments arranged by size:
1827 1015 749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 2470 2597 3591
Size: 940 1530 127 994
Fragments arranged by size:
1530 994 940 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1949 1979 3566 3591
Size: 1949 30 1587 25
Fragments arranged by size:
1949 1587 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2281 3591
Size: 2281 1310
BseYI C'CCAG_C
Cuts at: 0 144 3591
Size: 144 3447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1109 3591
Size: 524 585 2482
Fragments arranged by size:
2482 585 524
BsiHKAI G_wGCw'C
Cuts at: 0 2001 2486 3579 3591
Size: 2001 485 1093 12
Fragments arranged by size:
2001 1093 485 12
Bsp1286I G_dGCh'C
Cuts at: 0 2001 2091 2486 3579 3591
Size: 2001 90 395 1093 12
Fragments arranged by size:
2001 1093 395 90 12
BspHI T'CATG_A
Cuts at: 0 291 834 3591
Size: 291 543 2757
Fragments arranged by size:
2757 543 291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 2098 3324 3591
Size: 306 1792 1226 267
Fragments arranged by size:
1792 1226 306 267
BsrDI GCAATG_nn'
Cuts at: 0 1862 3591
Size: 1862 1729
BssSI C'ACGA_G
Cuts at: 0 1457 3591
Size: 1457 2134
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1192 3591
Size: 1192 2399
BstEII G'GTnAC_C
Cuts at: 0 2622 3591
Size: 2622 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 1914 3379 3483 3591
Size: 123 1791 1465 104 108
Fragments arranged by size:
1791 1465 123 108 104
BstYI r'GATC_y
Cuts at: 0 384 1533 3357 3591
Size: 384 1149 1824 234
Fragments arranged by size:
1824 1149 384 234
BstZ17I GTA'TAC
Cuts at: 0 883 3591
Size: 883 2708
Bsu36I CC'TnA_GG
Cuts at: 0 3219 3591
Size: 3219 372
BtsI GCAGTG_nn'
Cuts at: 0 2120 2158 3591
Size: 2120 38 1433
Fragments arranged by size:
2120 1433 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 299 334 3591
Size: 299 35 3257
Fragments arranged by size:
3257 299 35
EaeI y'GGCC_r
Cuts at: 0 2844 3591
Size: 2844 747
EarI CTCTTCn'nnn_
Cuts at: 0 82 394 596 2090 2165 2474 2612 2978
Size: 82 312 202 1494 75 309 138 366
Cuts at: 2978 3007 3313 3591
Size: 29 306 278
Fragments arranged by size:
1494 366 312 309 306 278 202 138
82 75 29
EcoICRI GAG'CTC
Cuts at: 0 2484 3591
Size: 2484 1107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 746 772 896 1130 1325 1655 1831
Size: 458 288 26 124 234 195 330 176
Cuts at: 1831 2182 2189 2792 2890 3002 3223 3591
Size: 351 7 603 98 112 221 368
Fragments arranged by size:
603 458 368 351 330 288 234 221
195 176 124 112 98 26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 3337 3591
Size: 163 3174 254
Fragments arranged by size:
3174 254 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 922 1207 1396 3234 3591
Size: 922 285 189 1838 357
Fragments arranged by size:
1838 922 357 285 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 625 657 2485 2517 3109 3141
Size: 469 32 124 32 1828 32 592 32
Cuts at: 3141 3591
Size: 450
Fragments arranged by size:
1828 592 469 450 124 32 32 32
32
HincII GTy'rAC
Cuts at: 0 414 3591
Size: 414 3177
HindIII A'AGCT_T
Cuts at: 0 784 3591
Size: 784 2807
HpaI GTT'AAC
Cuts at: 0 414 3591
Size: 414 3177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 179 637 3591
Size: 140 39 458 2954
Fragments arranged by size:
2954 458 140 39
MscI TGG'CCA
Cuts at: 0 2846 3591
Size: 2846 745
MslI CAynn'nnrTG
Cuts at: 0 1139 3424 3591
Size: 1139 2285 167
Fragments arranged by size:
2285 1139 167
MspA1I CmG'CkG
Cuts at: 0 505 748 1123 1153 1452 2575 3591
Size: 505 243 375 30 299 1123 1016
Fragments arranged by size:
1123 1016 505 375 299 243 30
NdeI CA'TA_TG
Cuts at: 0 1485 3591
Size: 1485 2106
NsiI A_TGCA'T
Cuts at: 0 5 2421 3591
Size: 5 2416 1170
Fragments arranged by size:
2416 1170 5
NspI r_CATG'y
Cuts at: 0 977 1081 1276 3583 3591
Size: 977 104 195 2307 8
Fragments arranged by size:
2307 977 195 104 8
PciI A'CATG_T
Cuts at: 0 3579 3591
Size: 3579 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1935 1967 3591
Size: 1935 32 1624
Fragments arranged by size:
1935 1624 32
PshAI GACnn'nnGTC
Cuts at: 0 55 3591
Size: 55 3536
PsiI TTA'TAA
Cuts at: 0 781 3591
Size: 781 2810
PvuII CAG'CTG
Cuts at: 0 505 748 1123 1452 2575 3591
Size: 505 243 375 329 1123 1016
Fragments arranged by size:
1123 1016 505 375 329 243
SacI G_AGCT'C
Cuts at: 0 2486 3591
Size: 2486 1105
SapI GCTCTTCn'nnn_
Cuts at: 0 2474 2978 3591
Size: 2474 504 613
Fragments arranged by size:
2474 613 504
SfcI C'TryA_G
Cuts at: 0 551 1329 3053 3497 3591
Size: 551 778 1724 444 94
Fragments arranged by size:
1724 778 551 444 94
SmlI C'TyrA_G
Cuts at: 0 955 2485 2612 3591
Size: 955 1530 127 979
Fragments arranged by size:
1530 979 955 127
SphI G_CATG'C
Cuts at: 0 1081 1276 3591
Size: 1081 195 2315
Fragments arranged by size:
2315 1081 195
SspI AAT'ATT
Cuts at: 0 343 3093 3591
Size: 343 2750 498
Fragments arranged by size:
2750 498 343
StyI C'CwwG_G
Cuts at: 0 1823 3353 3554 3591
Size: 1823 1530 201 37
Fragments arranged by size:
1823 1530 201 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 3582 3591
Size: 67 3515 9
Fragments arranged by size:
3515 67 9
TatI w'GTAC_w
Cuts at: 0 378 3591
Size: 378 3213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1710 1742 1949 1974 1981 2006 3566 3591
Size: 1710 32 207 25 7 25 1560 25
Fragments arranged by size:
1710 1560 207 32 25 25 25 7
XbaI T'CTAG_A
Cuts at: 0 21 1350 2091 3591
Size: 21 1329 741 1500
Fragments arranged by size:
1500 1329 741 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1544 3591
Size: 1544 2047
XmnI GAAnn'nnTTC
Cuts at: 0 3534 3591
Size: 3534 57
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AhdI AlfI AloI
AlwNI ApoI AseI BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI BplI BpmI Bpu10I
BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI
FalI Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NdeI NsiI NspI PciI PpiI PshAI PsiI
PvuII SacI SapI SfcI SmlI SphI SspI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AleI ApaI ApaLI
AscI AsiSI AvaI AvrII BanI BbeI BclI BglII
Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BstBI BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI Tth111I XhoI XmaI ZraI