################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 1108 AAATTC [GA]^AATT[CT] AflIII 374 ACGTGT A^C[GA][CT]GT AlwNI 466 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 452 AGCGCT AGC^GCT ApaI 29 GGGCCC GGGCC^C ApoI 1108 AAATTC [GA]^AATT[CT] Asp700I 231 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 18 GGCGCC GGCGC^C BciVI 330 GGATAC GTATCC(6/5) BfaI 169 CTAG C^TAG BsaAI 373 TACGTG [CT]AC^GT[GA] BsaI 1252 GAGACC GGTCTC(1/5) BsaWI 1442 ACCGGA [AT]^CCGG[AT] BsiEI 1440 CGACCG CG[GA][CT]^CG BsrDI 798 CATTGC GCAATG(2/0) BssHII 1306 GCGCGC G^CGCGC BstAPI 276 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 907 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 775 GACACCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 452 AGCGCT AGC^GCT HgaI 1230 GACGC GACGC(5/10) KasI 18 GGCGCC G^GCGCC MaeI 169 CTAG C^TAG MaeII 374 ACGT A^CGT MslI 1266 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 18 GGCGCC GG^CGCC PflMI 676 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1246 GACTC GAGTC(4/5) SapI 915 GCTCTTC GCTCTTC(1/4) ScaI 320 AGTACT AGT^ACT SfiI 1049 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 18 GGCGCC GGC^GCC Van91I 676 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1160 CCACTCGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 231 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 18 GGCGCC G[GA]^CG[CT]C 1230 GACGCC AlwI 258 GATCC GGATC(4/5) 468 GATCC BanI 18 GGCGCC G^G[CT][GA]CC 584 GGCACC BbsI 1002 GAAGAC GAAGAC(2/6) 1339 GAAGAC BglI 235 GCCTTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1050 GCCCCGCAGGC BlpI 480 GCTCAGC GC^T[ACGT]AGC 637 GCTGAGC Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC 637 GCTGAGC BpuAI 1002 GAAGAC GAAGAC(2/6) 1339 GAAGAC BsaHI 18 GGCGCC G[GA]^CG[CT]C 1230 GACGCC BsrBI 43 GAGCGG CCGCTC(-3/-3) 49 GAGCGG BstXI 762 CCAGACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1007 CCAAGGCCATGG BstYI 257 AGATCC [GA]^GATC[CT] 467 AGATCC CelII 480 GCTCAGC GC^T[ACGT]AGC 637 GCTGAGC EaeI 692 TGGCCG [CT]^GGCC[GA] 1454 TGGCCG EarI 901 CTCTTC CTCTTC(1/4) 916 CTCTTC EcoNI 344 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1428 CCTCCACGAGG Hin1I 18 GGCGCC G[GA]^CG[CT]C 1230 GACGCC Ksp632I 901 CTCTTC CTCTTC(1/4) 916 CTCTTC MaeIII 784 GTCAC ^GT[ACGT]AC 824 GTGAC MflI 257 AGATCC [GA]^GATC[CT] 467 AGATCC NaeI 15 GCCGGC GCC^GGC 878 GCCGGC NcoI 173 CCATGG C^CATGG 1013 CCATGG NgoMIV 15 GCCGGC G^CCGGC 878 GCCGGC NspI 143 ACATGC [GA]CATG^[CT] 833 ACATGC PstI 498 CTGCAG CTGCA^G 1066 CTGCAG PvuII 277 CAGCTG CAG^CTG 1357 CAGCTG SacI 211 GAGCTC GAGCT^C 1408 GAGCTC SfaNI 1272 GATGC GCATC(5/9) 1314 GCATC SfcI 498 CTGCAG C^T[GA][CT]AG 1066 CTGCAG SmlI 391 CTCAAG C^T[CT][GA]AG 1480 CTCAAG StuI 233 AGGCCT AGG^CCT 341 AGGCCT TfiI 590 GAATC G^A[AT]TC 1133 GAATC Tsp45I 784 GTCAC ^GT[GC]AC 824 GTGAC Tsp509I 398 AATT ^AATT 1109 AATT XhoII 257 AGATCC [GA]^GATC[CT] 467 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AfaI AluI Alw44I ApaLI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI