(Circular) (Six-base) MAPSORT of: pFN21AE3221.seq Check: 2260 from: 1 to: 6208 pFN21AE3221 6208 bp With 182 enzymes: SgfI * March 28, 2012 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 2960 4271 5655 5923 1632 Size: 512 816 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 816 512 268 AfeI AGC'GCT Cuts at: 2459 2459 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2379 5107 6084 1203 Size: 1176 2728 977 1327 Fragments arranged by size: 2728 1327 1176 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2974 1184 Size: 1790 4418 AlwNI CAG_nnn'CTG Cuts at: 2476 3951 5523 2476 Size: 1475 1572 3161 Fragments arranged by size: 3161 1572 1475 ApaI G_GGCC'C Cuts at: 2038 2038 Size: 6208 ApaLI G'TGCA_C Cuts at: 2464 2930 3419 4265 5421 2464 Size: 466 489 846 1156 3251 Fragments arranged by size: 3251 1156 846 489 466 ApoI r'AATT_y Cuts at: 1493 3113 3517 3743 3895 6004 1493 Size: 1620 404 226 152 2109 1697 Fragments arranged by size: 2109 1697 1620 404 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2445 2784 2868 3533 1870 Size: 73 94 408 339 84 665 4545 Fragments arranged by size: 4545 665 408 339 94 84 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2023 2589 3529 4984 618 Size: 325 217 627 236 566 940 1455 1842 Fragments arranged by size: 1842 1455 940 627 566 325 236 217 BanII G_rGCy'C Cuts at: 1391 2038 2220 3246 3417 3527 1391 Size: 647 182 1026 171 110 4072 Fragments arranged by size: 4072 1026 647 182 171 110 BbeI G_GCGC'C Cuts at: 2027 2027 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3014 3351 928 Size: 971 1115 337 3785 Fragments arranged by size: 3785 1115 971 337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2329 4100 5310 2329 Size: 1771 1210 3227 Fragments arranged by size: 3227 1771 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2246 3061 Size: 107 122 71 631 605 337 237 815 Cuts at: 3061 4819 136 Size: 1758 1525 Fragments arranged by size: 1758 1525 815 631 605 337 237 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 2486 2643 3625 2486 Size: 157 982 5069 Fragments arranged by size: 5069 982 157 Bme1580I G_kGCm'C Cuts at: 1790 2038 2468 2934 3423 4269 5425 1790 Size: 248 430 466 489 846 1156 2573 Fragments arranged by size: 2573 1156 846 489 466 430 248 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2950 2950 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2765 3140 3311 3491 4868 1239 Size: 872 654 375 171 180 1377 2579 Fragments arranged by size: 2579 1377 872 654 375 180 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 3470 4333 5198 5496 2381 Size: 1089 863 865 298 3093 Fragments arranged by size: 3093 1089 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3251 4871 882 Size: 617 1752 1620 2219 Fragments arranged by size: 2219 1752 1620 617 BsaAI yAC'GTr Cuts at: 493 1204 2380 3999 493 Size: 711 1176 1619 2702 Fragments arranged by size: 2702 1619 1176 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 3236 4015 4397 Size: 53 83 186 936 491 1212 779 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1451 1212 936 779 635 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3447 4641 5039 5313 5460 5871 3447 Size: 1194 398 274 147 411 3784 Fragments arranged by size: 3784 1194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 1178 Size: 30 165 30 1305 30 4648 Fragments arranged by size: 4648 1305 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2270 2810 2894 3017 3224 1186 Size: 1018 66 540 84 123 207 4170 Fragments arranged by size: 4170 1018 540 207 123 84 66 BseYI C'CCAG_C Cuts at: 5411 5907 5411 Size: 496 5712 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 2651 3011 3338 1163 Size: 573 744 171 360 327 4033 Fragments arranged by size: 4033 744 573 360 327 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 3658 4419 5447 1540 Size: 275 186 1447 210 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 210 186 BsiHKAI G_wGCw'C Cuts at: 2168 2220 2468 2922 2934 3417 3423 3479 4269 Size: 52 248 454 12 483 6 56 790 Cuts at: 4269 4354 5425 2168 Size: 85 1071 2951 Fragments arranged by size: 2951 1071 790 483 454 248 85 56 52 12 6 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2168 2220 2468 2922 2934 3246 Size: 399 248 130 52 248 454 12 312 Cuts at: 3246 3417 3423 3479 3527 4269 4354 5425 1391 Size: 171 6 56 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 312 248 248 171 130 85 56 52 48 12 6 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2673 3033 3384 3563 844 Size: 1278 551 360 351 179 3489 Fragments arranged by size: 3489 1278 551 360 351 179 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2056 4095 1394 Size: 429 227 6 2039 3507 Fragments arranged by size: 3507 2039 429 227 6 BsrDI GCAATG_nn' Cuts at: 65 1248 2802 4703 4877 65 Size: 1183 1554 1901 174 1396 Fragments arranged by size: 1901 1554 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2883 3348 3357 4852 5039 1700 Size: 110 210 863 465 9 1495 187 2869 Fragments arranged by size: 2869 1495 863 465 210 187 110 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 3311 1925 Size: 1386 4822 BssSI C'ACGA_G Cuts at: 2252 3416 3437 4268 5280 2252 Size: 1164 21 831 1012 3180 Fragments arranged by size: 3180 1164 1012 831 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 2287 794 Size: 1493 4715 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 2774 3019 2774 Size: 245 5963 BstYI r'GATC_y Cuts at: 1972 2262 2472 3538 3905 4290 4307 5759 5845 Size: 290 210 1066 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1066 385 367 346 290 210 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2178 2521 3018 3493 513 Size: 552 939 174 343 497 475 3228 Fragments arranged by size: 3228 939 552 497 475 343 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5469 Fragments arranged by size: 5469 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3080 3287 3724 4518 4538 2956 Size: 124 207 437 794 20 4626 Fragments arranged by size: 4626 794 437 207 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3475 3510 529 Size: 35 2911 35 3227 Fragments arranged by size: 3227 2911 35 35 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 2917 2917 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2786 5215 809 Size: 507 1470 2429 1802 Fragments arranged by size: 2429 1802 1470 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2697 3459 3655 Size: 54 1425 50 275 18 867 762 196 Cuts at: 3655 4544 6173 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 867 762 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2912 2927 4138 1353 Size: 1559 15 1211 3423 Fragments arranged by size: 3423 1559 1211 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3056 4795 5169 5315 387 Size: 1064 138 1467 1739 374 146 1280 Fragments arranged by size: 1739 1467 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 3415 2218 Size: 1197 5011 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2144 2765 2960 3140 3311 3491 Size: 393 479 33 621 195 180 171 180 Cuts at: 3491 4271 4868 5655 5923 1239 Size: 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 621 597 479 393 268 195 180 180 171 33 EcoNI CCTnn'n_nnAGG Cuts at: 2353 3437 2353 Size: 1084 5124 EcoO109I rG'GnC_Cy Cuts at: 2872 3063 3400 3498 2872 Size: 191 337 98 5582 Fragments arranged by size: 5582 337 191 98 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2027 2461 3396 5355 1678 Size: 349 434 935 1959 2531 Fragments arranged by size: 2531 1959 935 434 349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2366 2398 2708 2740 3392 Size: 32 135 32 794 32 310 32 652 Cuts at: 3392 3424 4849 4881 4923 4955 1373 Size: 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 794 652 310 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2023 2023 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4386 Fragments arranged by size: 4386 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3275 4166 4525 4684 518 Size: 648 2109 891 359 159 2042 Fragments arranged by size: 2109 2042 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2284 2711 3070 3364 3624 4301 5449 5694 Size: 678 427 359 294 260 677 1148 245 Cuts at: 5694 1606 Size: 2120 Fragments arranged by size: 2120 1148 678 677 427 359 294 260 245 NaeI GCC'GGC Cuts at: 2022 2885 2022 Size: 863 5345 NarI GG'CG_CC Cuts at: 2024 2024 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 2004 2178 3018 513 Size: 552 939 174 840 3703 Fragments arranged by size: 3703 939 840 552 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2020 2883 2020 Size: 863 5345 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2152 2842 3566 5111 1132 Size: 97 923 690 724 1545 2229 Fragments arranged by size: 2229 1545 923 724 690 97 PasI CC'CwG_GG Cuts at: 1348 3175 1348 Size: 1827 4381 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 2687 1108 Size: 1579 4629 PfoI T'CCnGG_A Cuts at: 2088 3117 3468 2088 Size: 1029 351 4828 Fragments arranged by size: 4828 1029 351 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2872 3400 3498 2872 Size: 528 98 5582 Fragments arranged by size: 5582 528 98 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 2034 2034 Size: 6208 PstI C_TGCA'G Cuts at: 830 2507 3075 3560 830 Size: 1677 568 485 3478 Fragments arranged by size: 3478 1677 568 485 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2284 3364 2284 Size: 1080 5128 SacI G_AGCT'C Cuts at: 2220 3417 2220 Size: 1197 5011 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SanDI GG'GwC_CC Cuts at: 2872 3498 2872 Size: 626 5582 SapI GCTCTTCn'nnn_ Cuts at: 2927 2927 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 2327 4456 6168 2327 Size: 2129 1712 2367 Fragments arranged by size: 2367 2129 1712 SfcI C'TryA_G Cuts at: 826 1045 2503 3071 3556 4691 5372 5563 826 Size: 219 1458 568 485 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 681 568 485 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3061 3061 Size: 6208 SfoI GGC'GCC Cuts at: 2025 2025 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2039 2447 2786 2870 3535 1872 Size: 167 408 339 84 665 4545 Fragments arranged by size: 4545 665 408 339 167 84 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 3485 4312 5213 5475 820 Size: 197 926 453 1089 827 901 262 1553 Fragments arranged by size: 1553 1089 926 901 827 453 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SrfI GCCC'GGGC Cuts at: 2447 2447 Size: 6208 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2240 2348 1517 Size: 723 108 5377 Fragments arranged by size: 5377 723 108 StyI C'CwwG_G Cuts at: 513 1065 2004 2140 2178 3012 3018 3647 513 Size: 552 939 136 38 834 6 629 3074 Fragments arranged by size: 3074 939 834 629 552 136 38 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4235 4252 4405 4590 5035 325 Size: 1255 2655 17 153 185 445 1498 Fragments arranged by size: 2655 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2755 4454 Size: 274 80 33 51 1443 348 430 1699 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1712 1699 1443 430 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 6168 6200 2087 Size: 32 4049 32 2095 Fragments arranged by size: 4049 2095 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3172 3172 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2037 2445 2784 2868 3533 1870 Size: 167 408 339 84 665 4545 Fragments arranged by size: 4545 665 408 339 167 84 XmnI GAAnn'nnTTC Cuts at: 2240 4337 2240 Size: 2097 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SwaI