Restriction map for
pFN21AE3221
(Linear) (Six-base) MAPSORT of: KIEE3221.seq Check: 2265 from: 1 to: 1503
KIEE3221.orf
With 182 enzymes: SgfI *
March 22, 2012 16:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 955 1503
Size: 139 816 548
Fragments arranged by size:
816 548 139
AfeI AGC'GCT
Cuts at: 0 454 1503
Size: 454 1049
AflIII A'CryG_T
Cuts at: 0 374 1503
Size: 374 1129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 969 1503
Size: 969 534
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1503
Size: 471 1032
ApaI G_GGCC'C
Cuts at: 0 33 1503
Size: 33 1470
ApaLI G'TGCA_C
Cuts at: 0 459 925 1414 1503
Size: 459 466 489 89
Fragments arranged by size:
489 466 459 89
ApoI r'AATT_y
Cuts at: 0 1108 1503
Size: 1108 395
AvaI C'yCGr_G
Cuts at: 0 32 440 779 863 1503
Size: 32 408 339 84 640
Fragments arranged by size:
640 408 339 84 32
BanI G'GyrC_C
Cuts at: 0 18 584 1503
Size: 18 566 919
Fragments arranged by size:
919 566 18
BanII G_rGCy'C
Cuts at: 0 33 215 1241 1412 1503
Size: 33 182 1026 171 91
Fragments arranged by size:
1026 182 171 91 33
BbeI G_GCGC'C
Cuts at: 0 22 1503
Size: 22 1481
BbsI GAAGACnn'nnnn_
Cuts at: 0 1009 1346 1503
Size: 1009 337 157
Fragments arranged by size:
1009 337 157
BciVI GTATCCnnnnn_n'
Cuts at: 0 324 1503
Size: 324 1179
BglI GCCn_nnn'nGGC
Cuts at: 0 241 1056 1503
Size: 241 815 447
Fragments arranged by size:
815 447 241
BlpI GC'TnA_GC
Cuts at: 0 481 638 1503
Size: 481 157 865
Fragments arranged by size:
865 481 157
Bme1580I G_kGCm'C
Cuts at: 0 33 463 929 1418 1503
Size: 33 430 466 489 85
Fragments arranged by size:
489 466 430 85 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 945 1503
Size: 945 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 760 1135 1306 1486 1503
Size: 106 654 375 171 180 17
Fragments arranged by size:
654 375 180 171 106 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1465 1503
Size: 376 1089 38
Fragments arranged by size:
1089 376 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 1246 1503
Size: 1246 257
BsaAI yAC'GTr
Cuts at: 0 375 1503
Size: 375 1128
BsaHI Gr'CG_yC
Cuts at: 0 19 1231 1503
Size: 19 1212 272
Fragments arranged by size:
1212 272 19
BsaWI w'CCGG_w
Cuts at: 0 1442 1503
Size: 1442 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 1503
Size: 703 30 770
Fragments arranged by size:
770 703 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 265 805 889 1012 1219 1503
Size: 199 66 540 84 123 207 284
Fragments arranged by size:
540 284 207 199 123 84 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 646 1006 1333 1503
Size: 475 171 360 327 170
Fragments arranged by size:
475 360 327 171 170
BsiEI CG_ry'CG
Cuts at: 0 1443 1503
Size: 1443 60
BsiHKAI G_wGCw'C
Cuts at: 0 163 215 463 917 929 1412 1418 1474
Size: 163 52 248 454 12 483 6 56
Cuts at: 1474 1503
Size: 29
Fragments arranged by size:
483 454 248 163 56 52 29 12
6
Bsp1286I G_dGCh'C
Cuts at: 0 33 163 215 463 917 929 1241 1412
Size: 33 130 52 248 454 12 312 171
Cuts at: 1412 1418 1474 1503
Size: 6 56 29
Fragments arranged by size:
454 312 248 171 130 56 52 33
29 12 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 668 1028 1379 1503
Size: 117 551 360 351 124
Fragments arranged by size:
551 360 351 124 117
BsrBI CCG'CTC
Cuts at: 0 45 51 1503
Size: 45 6 1452
Fragments arranged by size:
1452 45 6
BsrDI GCAATG_nn'
Cuts at: 0 797 1503
Size: 797 706
BsrFI r'CCGG_y
Cuts at: 0 15 878 1343 1352 1503
Size: 15 863 465 9 151
Fragments arranged by size:
863 465 151 15 9
BssHII G'CGCG_C
Cuts at: 0 1306 1503
Size: 1306 197
BssSI C'ACGA_G
Cuts at: 0 247 1411 1432 1503
Size: 247 1164 21 71
Fragments arranged by size:
1164 247 71 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 282 1503
Size: 282 1221
BstXI CCAn_nnnn'nTGG
Cuts at: 0 769 1014 1503
Size: 769 245 489
Fragments arranged by size:
769 489 245
BstYI r'GATC_y
Cuts at: 0 257 467 1503
Size: 257 210 1036
Fragments arranged by size:
1036 257 210
BtgI C'CryG_G
Cuts at: 0 173 516 1013 1488 1503
Size: 173 343 497 475 15
Fragments arranged by size:
497 475 343 173 15
BtsI GCAGTG_nn'
Cuts at: 0 951 1075 1282 1503
Size: 951 124 207 221
Fragments arranged by size:
951 221 207 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1470 1503
Size: 1470 33
DraIII CAC_nnn'GTG
Cuts at: 0 912 1503
Size: 912 591
DrdI GACnn_nn'nnGTC
Cuts at: 0 781 1503
Size: 781 722
EaeI y'GGCC_r
Cuts at: 0 692 1454 1503
Size: 692 762 49
Fragments arranged by size:
762 692 49
EarI CTCTTCn'nnn_
Cuts at: 0 907 922 1503
Size: 907 15 581
Fragments arranged by size:
907 581 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1051 1503
Size: 1051 452
EcoICRI GAG'CTC
Cuts at: 0 213 1410 1503
Size: 213 1197 93
Fragments arranged by size:
1197 213 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 139 760 955 1135 1306 1486 1503
Size: 106 33 621 195 180 171 180 17
Fragments arranged by size:
621 195 180 180 171 106 33 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 348 1432 1503
Size: 348 1084 71
Fragments arranged by size:
1084 348 71
EcoO109I rG'GnC_Cy
Cuts at: 0 867 1058 1395 1493 1503
Size: 867 191 337 98 10
Fragments arranged by size:
867 337 191 98 10
HaeII r_GCGC'y
Cuts at: 0 22 456 1391 1503
Size: 22 434 935 112
Fragments arranged by size:
935 434 112 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 703 735 1387 1419 1503
Size: 361 32 310 32 652 32 84
Fragments arranged by size:
652 361 310 84 32 32 32
KasI G'GCGC_C
Cuts at: 0 18 1503
Size: 18 1485
MslI CAynn'nnrTG
Cuts at: 0 1270 1503
Size: 1270 233
MspA1I CmG'CkG
Cuts at: 0 279 706 1065 1359 1503
Size: 279 427 359 294 144
Fragments arranged by size:
427 359 294 279 144
NaeI GCC'GGC
Cuts at: 0 17 880 1503
Size: 17 863 623
Fragments arranged by size:
863 623 17
NarI GG'CG_CC
Cuts at: 0 19 1503
Size: 19 1484
NcoI C'CATG_G
Cuts at: 0 173 1013 1503
Size: 173 840 490
Fragments arranged by size:
840 490 173
NgoMIV G'CCGG_C
Cuts at: 0 15 878 1503
Size: 15 863 625
Fragments arranged by size:
863 625 15
NspI r_CATG'y
Cuts at: 0 147 837 1503
Size: 147 690 666
Fragments arranged by size:
690 666 147
PasI CC'CwG_GG
Cuts at: 0 1170 1503
Size: 1170 333
PflMI CCAn_nnn'nTGG
Cuts at: 0 682 1503
Size: 682 821
PfoI T'CCnGG_A
Cuts at: 0 83 1112 1463 1503
Size: 83 1029 351 40
Fragments arranged by size:
1029 351 83 40
PpuMI rG'GwC_Cy
Cuts at: 0 867 1395 1493 1503
Size: 867 528 98 10
Fragments arranged by size:
867 528 98 10
PspOMI G'GGCC_C
Cuts at: 0 29 1503
Size: 29 1474
PstI C_TGCA'G
Cuts at: 0 502 1070 1503
Size: 502 568 433
Fragments arranged by size:
568 502 433
PvuII CAG'CTG
Cuts at: 0 279 1359 1503
Size: 279 1080 144
Fragments arranged by size:
1080 279 144
SacI G_AGCT'C
Cuts at: 0 215 1412 1503
Size: 215 1197 91
Fragments arranged by size:
1197 215 91
SanDI GG'GwC_CC
Cuts at: 0 867 1493 1503
Size: 867 626 10
Fragments arranged by size:
867 626 10
SapI GCTCTTCn'nnn_
Cuts at: 0 922 1503
Size: 922 581
ScaI AGT'ACT
Cuts at: 0 322 1503
Size: 322 1181
SfcI C'TryA_G
Cuts at: 0 498 1066 1503
Size: 498 568 437
Fragments arranged by size:
568 498 437
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1056 1503
Size: 1056 447
SfoI GGC'GCC
Cuts at: 0 20 1503
Size: 20 1483
SmaI CCC'GGG
Cuts at: 0 34 442 781 865 1503
Size: 34 408 339 84 638
Fragments arranged by size:
638 408 339 84 34
SmlI C'TyrA_G
Cuts at: 0 391 1480 1503
Size: 391 1089 23
Fragments arranged by size:
1089 391 23
SrfI GCCC'GGGC
Cuts at: 0 442 1503
Size: 442 1061
StuI AGG'CCT
Cuts at: 0 235 343 1503
Size: 235 108 1160
Fragments arranged by size:
1160 235 108
StyI C'CwwG_G
Cuts at: 0 135 173 1007 1013 1503
Size: 135 38 834 6 490
Fragments arranged by size:
834 490 135 38 6
TatI w'GTAC_w
Cuts at: 0 320 750 1503
Size: 320 430 753
Fragments arranged by size:
753 430 320
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 1503
Size: 82 32 1389
Fragments arranged by size:
1389 82 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 1503
Size: 1167 336
XmaI C'CCGG_G
Cuts at: 0 32 440 779 863 1503
Size: 32 408 339 84 640
Fragments arranged by size:
640 408 339 84 32
XmnI GAAnn'nnTTC
Cuts at: 0 235 1503
Size: 235 1268
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BciVI BglI BlpI
Bme1580I BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI
SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BclI BfrBI BglII BmgBI BmrI BmtI
Bpu10I BsaBI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
EagI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TaqII Tth111I XbaI XhoI ZraI