################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TfiI Tru9I TseI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AluI 159 AGCT AG^CT Alw44I 273 GTGCAC G^TGCAC AlwI 292 GATCC GGATC(4/5) AlwNI 97 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 273 GTGCAC G^TGCAC AspHI 273 GTGCAC G[AT]GC[AT]^C BbsI 15 GAAGAC GAAGAC(2/6) BclI 110 TGATCA T^GATCA BfaI 47 CTAG C^TAG BpmI 31 CTGGAG CTGGAG(16/14) BpuAI 15 GAAGAC GAAGAC(2/6) BsaJI 84 CCTGGG C^C[ACGT][ACGT]GG BsiHKAI 273 GTGCAC G[AT]GC[AT]^C BstAPI 245 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 18 GACGAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 5 TGGCCG [CT]^GGCC[GA] FbaI 110 TGATCA T^GATCA Fnu4HI 7 GCCGC GC^[ACGT]GC HaeIII 6 GGCC GG^CC ItaI 7 GCCGC GC^[ACGT]GC MaeI 47 CTAG C^TAG MaeII 80 ACGT A^CGT MaeIII 299 GTGAC ^GT[ACGT]AC MnlI 165 GAGG CCTC(7/6) MslI 217 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 50 GGCTCC GG[ACGT]^[ACGT]CC ScaI 182 AGTACT AGT^ACT SspI 232 AATATT AAT^ATT TaqI 37 TCGA T^CGA Tsp45I 299 GTGAC ^GT[GC]AC Tsp509I 131 AATT ^AATT TthHB8I 37 TCGA T^CGA XcmI 213 CCATCACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 8 CCGC CCGC(-3/-1) 254 GCGG BmyI 243 GGGCAC G[AGT]GC[ACT]^C 273 GTGCAC Bsp1286I 243 GGGCAC G[AGT]GC[ACT]^C 273 GTGCAC BssKI 84 CCTGG ^CC[ACGT]GG 295 CCAGG BstNI 84 CCTGG CC^[AT]GG 295 CCAGG Cac8I 250 GCATGC GC[ACGT]^[ACGT]GC 320 GCATGC EcoRII 84 CCTGG ^CC[AT]GG 295 CCAGG HphI 298 GGTGA GGTGA(8/7) 337 GGTGA MboII 15 GAAGA GAAGA(8/7) 105 GAAGA MvaI 84 CCTGG CC^[AT]GG 295 CCAGG MwoI 190 GCTATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 245 GCACAGCATGC NlaIII 251 CATG CATG^ 321 CATG NspI 250 GCATGC [GA]CATG^[CT] 320 GCATGC ScrFI 84 CCTGG CC^[ACGT]GG 295 CCAGG SfcI 219 CTACAG C^T[GA][CT]AG 264 CTACAG SphI 250 GCATGC GCATG^C 320 GCATGC TspRI 222 CAGTG CA[GC]TG[ACGT][ACGT]^ 278 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AfaI BsrI DpnII HinfI MboI NdeII PleI RsaI Sau3AI