##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MscI        MslI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccIII        351  TCCGGA               T^CCGGA
AfaI          187  GTAC                 GT^AC
AlwI          390  GGATC                GGATC(4/5)
AspHI          28  GAGCAC               G[AT]GC[AT]^C
BbsI          355  GAAGAC               GAAGAC(2/6)
BpuAI         355  GAAGAC               GAAGAC(2/6)
BsaJI         381  CCGGGG               C^C[ACGT][ACGT]GG
BsaWI         351  TCCGGA               [AT]^CCGG[AT]
BseAI         351  TCCGGA               T^CCGGA
BseRI          25  GAGGAG               GAGGAG(10/8)
BsgI          159  GTGCAG               GTGCAG(16/14)
BsiHKAI        28  GAGCAC               G[AT]GC[AT]^C
BspEI         351  TCCGGA               T^CCGGA
BspHI         174  TCATGA               T^CATGA
BsrI          136  ACTGG                ACTGG(1/-1)
BstF5I        378  CATCC                GGATG(2/0)
DdeI          365  CTAAG                C^T[ACGT]AG
DraI          313  TTTAAA               TTT^AAA
FauI          396  CCCGC                CCCGC(4/6)
FokI          378  CATCC                GGATG(9/13)
HaeIII        102  GGCC                 GG^CC
HinfI         327  GATTC                G^A[ACGT]TC
HphI          393  TCACC                GGTGA(8/7)
MroI          351  TCCGGA               T^CCGGA
RcaI          174  TCATGA               T^CATGA
RsaI          187  GTAC                 GT^AC
TfiI          327  GATTC                G^A[AT]TC
Tsp45I         80  GTGAC                ^GT[GC]AC
TspRI          78  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          242  CCAAAGAGTGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI          397  CCGC                 CCGC(-3/-1)
                4  GCGG                
AcsI          123  GAATTT               [GA]^AATT[CT]
              310  AAATTT              
ApoI          123  GAATTT               [GA]^AATT[CT]
              310  AAATTT              
BanII         106  GGGCTC               G[GA]GC[CT]^C
              260  GGGCTC              
BcnI          104  CCGGG                CC^[GC]GG
              381  CCGGG               
BfaI           22  CTAG                 C^TAG
              265  CTAG                
BpmI           34  CTGGAG               CTGGAG(16/14)
              253  CTGGAG              
MaeI           22  CTAG                 C^TAG
              265  CTAG                
MaeIII         80  GTGAC                ^GT[ACGT]AC
              133  GTTAC               
MboII         306  GAAGA                GAAGA(8/7)
              355  GAAGA               
MseI          284  TTAA                 T^TAA
              314  TTAA                
NciI          104  CCGGG                CC^[GC]GG
              381  CCGGG               
SfaNI          68  GCATC                GCATC(5/9)
              377  GCATC               
Tru9I         284  TTAA                 T^TAA
              314  TTAA                
Tsp509I       124  AATT                 ^AATT
              311  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        BbvI        BmyI        BsiYI       BslI        
Bsp1286I    BssKI       BstNI       DpnII       EcoRII      
Fnu4HI      HapII       HpaII       ItaI        MboI        
MnlI        MspI        MvaI        NdeII       NlaIII      
Sau3AI      ScrFI       TaqI        TseI        TthHB8I