################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 866 CCGC CCGC(-3/-1) AvrII 615 CCTAGG C^CTAGG BalI 201 TGGCCA TGG^CCA BanI 4 GGCACC G^G[CT][GA]CC BbsI 388 GAAGAC GAAGAC(2/6) BclI 372 TGATCA T^GATCA BlnI 615 CCTAGG C^CTAGG BmyI 3 GGGCAC G[AGT]GC[ACT]^C BpuAI 388 GAAGAC GAAGAC(2/6) BsaBI 478 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 934 GAGGAG GAGGAG(10/8) BsgI 821 GTGCAG GTGCAG(16/14) BsiEI 948 CGGCCG CG[GA][CT]^CG BsmAI 749 GAGAC GTCTC(1/5) BsmI 113 GCATTC GAATGC(1/-1) Bsp1286I 3 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 859 TGTACA T^GTACA BspDI 160 ATCGAT AT^CGAT BspHI 102 TCATGA T^CATGA BsrDI 123 CATTGC GCAATG(2/0) BsrGI 859 TGTACA T^GTACA BstXI 432 CCATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 829 GGATCT [GA]^GATC[CT] ClaI 160 ATCGAT AT^CGAT DraII 176 AGGGCCT [GA]G^G[ACGT]CC[CT] EagI 948 CGGCCG C^GGCCG EarI 73 GAAGAG CTCTTC(1/4) EclXI 948 CGGCCG C^GGCCG Eco52I 948 CGGCCG C^GGCCG EcoO109I 176 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 478 GATATC GAT^ATC FbaI 372 TGATCA T^GATCA Ksp632I 73 GAAGAG CTCTTC(1/4) MaeII 251 ACGT A^CGT MamI 478 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 829 GGATCT [GA]^GATC[CT] MluNI 201 TGGCCA TGG^CCA MscI 201 TGGCCA TGG^CCA MseI 23 TTAA T^TAA PflMI 38 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 318 GAGTC GAGTC(4/5) PshAI 246 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 212 CTGCAG CTGCA^G RcaI 102 TCATGA T^CATGA SexAI 258 ACCAGGT A^CC[AT]GGT SfcI 212 CTGCAG C^T[GA][CT]AG SspBI 859 TGTACA T^GTACA Tru9I 23 TTAA T^TAA Van91I 38 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 791 CCAAGGGAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 829 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AlwI 829 GGATC GGATC(4/5) 877 GGATC BstEII 582 GGTCACC G^GT[ACGT]ACC 1053 GGTGACC BstPI 582 GGTCACC G^GT[ACGT]ACC 1053 GGTGACC Cfr13I 177 GGGCC G^G[ACGT]CC 235 GGGCC DdeI 316 CTGAG C^T[ACGT]AG 458 CTCAG EaeI 201 TGGCCA [CT]^GGCC[GA] 948 CGGCCG EcoO65I 582 GGTCACC G^GT[ACGT]ACC 1053 GGTGACC MboII 73 GAAGA GAAGA(8/7) 388 GAAGA Sau96I 177 GGGCC G^G[ACGT]CC 235 GGGCC TaqI 161 TCGA T^CGA 595 TCGA TthHB8I 161 TCGA T^CGA 595 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BspMI BsrI BssKI BstF5I BstNI Cac8I DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI StyI TfiI TseI Tsp45I Tsp509I TspRI