(Circular) (Six-base) MAPSORT of: pFN21AE4362.seq Check: 9855 from: 1 to: 5845 pFN21AE4362 5845 bp With 182 enzymes: SgfI * March 28, 2012 13:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2819 2819 Size: 5845 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3908 5292 5560 1632 Size: 2276 1384 268 1917 Fragments arranged by size: 2276 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 2474 1166 Size: 1308 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 3588 5160 3588 Size: 1572 4273 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2174 2335 2515 3038 3154 3380 3532 5641 Size: 681 161 180 523 116 226 152 2109 Cuts at: 5641 1493 Size: 1697 Fragments arranged by size: 2109 1697 681 523 226 180 161 152 116 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 3170 1870 Size: 73 1227 4545 Fragments arranged by size: 4545 1227 73 AvrII C'CTAG_G Cuts at: 2620 2620 Size: 5845 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 3166 4621 618 Size: 325 217 627 222 1157 1455 1842 Fragments arranged by size: 1842 1455 1157 627 325 222 217 BanII G_rGCy'C Cuts at: 1391 3164 1391 Size: 1773 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 928 Size: 971 501 4373 Fragments arranged by size: 4373 971 501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 2377 1548 Size: 829 5016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2012 3906 5062 1790 Size: 222 1894 1156 2573 Fragments arranged by size: 2573 1894 1156 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2189 3576 4534 Size: 416 53 83 399 24 772 1387 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1387 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 2487 5839 2487 Size: 3352 2493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 1186 Size: 1768 4077 BseYI C'CCAG_C Cuts at: 2877 5048 5544 2877 Size: 2171 496 3178 Fragments arranged by size: 3178 2171 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2847 1163 Size: 573 1111 4161 Fragments arranged by size: 4161 1111 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2956 3295 4056 5084 1540 Size: 275 186 955 339 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 339 275 186 BsiHKAI G_wGCw'C Cuts at: 3906 3991 5062 3906 Size: 85 1071 4689 Fragments arranged by size: 4689 1071 85 BsmI GAATG_Cn' Cuts at: 2118 3356 3449 2118 Size: 1238 93 4514 Fragments arranged by size: 4514 1238 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3164 3906 3991 5062 1391 Size: 399 222 1152 742 85 1071 2174 Fragments arranged by size: 2174 1152 1071 742 399 222 85 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 2107 3734 5464 2107 Size: 1627 1730 2488 Fragments arranged by size: 2488 1730 1627 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 2397 2423 2819 3200 844 Size: 1366 187 26 396 381 3489 Fragments arranged by size: 3489 1366 396 381 187 26 BsrBI CCG'CTC Cuts at: 1394 1823 3732 1394 Size: 429 1909 3507 Fragments arranged by size: 3507 1909 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 4340 4514 65 Size: 1183 879 2213 174 1396 Fragments arranged by size: 2213 1396 1183 879 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 2864 96 Size: 2768 3077 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 2587 3058 1184 Size: 1403 471 3971 Fragments arranged by size: 3971 1403 471 BstXI CCAn_nnnn'nTGG Cuts at: 2444 2444 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2834 3175 3542 3927 3944 5396 5482 5494 Size: 862 341 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 862 385 367 346 341 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2029 487 Size: 683 56 803 4303 Fragments arranged by size: 4303 803 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 2166 1554 Size: 612 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2953 3292 Size: 54 1425 50 275 18 376 747 339 Cuts at: 3292 4181 5810 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 747 376 339 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2953 3292 1537 Size: 275 1141 339 4090 Fragments arranged by size: 4090 1141 339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 3775 1353 Size: 720 1702 3423 Fragments arranged by size: 3423 1702 720 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3908 4505 5292 5560 1239 Size: 393 2276 597 787 268 1524 Fragments arranged by size: 2276 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2182 2182 Size: 5845 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 EcoRV GAT'ATC Cuts at: 2485 2485 Size: 5845 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 4486 4518 4560 Size: 32 135 32 943 32 1939 32 42 Cuts at: 4560 4592 1373 Size: 32 2626 Fragments arranged by size: 2626 1939 943 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2966 4959 5143 1305 Size: 1661 1993 184 2007 Fragments arranged by size: 2007 1993 1661 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2208 10 Size: 54 1425 343 376 3647 Fragments arranged by size: 3647 1425 376 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 2296 2474 3803 4162 4321 518 Size: 648 985 145 178 1329 359 159 2042 Fragments arranged by size: 2042 1329 985 648 359 178 159 145 MspA1I CmG'CkG Cuts at: 1606 3261 3938 5086 5331 1606 Size: 1655 677 1148 245 2120 Fragments arranged by size: 2120 1655 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 2725 1348 Size: 1377 4468 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2049 1108 Size: 941 4904 PfoI T'CCnGG_A Cuts at: 2772 2772 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2255 1613 Size: 642 5203 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PstI C_TGCA'G Cuts at: 830 2221 3197 830 Size: 1391 976 3478 Fragments arranged by size: 3478 1391 976 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SexAI A'CCwGG_T Cuts at: 2263 2263 Size: 5845 SfcI C'TryA_G Cuts at: 826 1045 2217 3193 4328 5009 5200 826 Size: 219 1172 976 1135 681 191 1471 Fragments arranged by size: 1471 1172 1135 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2004 2013 2100 2583 2620 2796 3112 Size: 552 939 9 87 483 37 176 316 Cuts at: 3112 3284 513 Size: 172 3074 Fragments arranged by size: 3074 939 552 483 316 176 172 87 37 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2453 2466 2864 Size: 274 80 33 51 1443 476 13 398 Cuts at: 2864 2888 4091 5803 96 Size: 24 1203 1712 138 Fragments arranged by size: 1712 1443 1203 476 398 274 138 80 51 33 24 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 2803 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI