################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AciI 312 GCGG CCGC(-3/-1) AcsI 323 GAATTC [GA]^AATT[CT] ApoI 323 GAATTC [GA]^AATT[CT] AvaI 339 CTCGGG C^[CT]CG[GA]G BalI 35 TGGCCA TGG^CCA BanI 159 GGCACC G^G[CT][GA]CC BmyI 158 GGGCAC G[AGT]GC[ACT]^C BseRI 69 CTCCTC GAGGAG(10/8) BsgI 121 GTGCAG GTGCAG(16/14) BsoBI 339 CTCGGG C^[CT]CG[GA]G Bsp1286I 158 GGGCAC G[AGT]GC[ACT]^C EaeI 35 TGGCCA [CT]^GGCC[GA] EarI 318 GAAGAG CTCTTC(1/4) EcoRI 323 GAATTC G^AATTC HphI 362 TCACC GGTGA(8/7) Ksp632I 318 GAAGAG CTCTTC(1/4) MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA MseI 251 TTAA T^TAA MspA1I 133 CAGCTG C[AC]G^C[GT]G NlaIII 54 CATG CATG^ NlaIV 159 GGCACC GG[ACGT]^[ACGT]CC NspI 53 GCATGC [GA]CATG^[CT] PleI 150 GAGTC GAGTC(4/5) PvuII 133 CAGCTG CAG^CTG SfaNI 273 GCATC GCATC(5/9) SmlI 127 CTTGAG C^T[CT][GA]AG SphI 53 GCATGC GCATG^C Tru9I 251 TTAA T^TAA Tsp509I 324 AATT ^AATT ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 97 GATCC GGATC(4/5) 372 GGATC AocI 71 CCTCAGG CC^T[ACGT]AGG 178 CCTAAGG BpmI 222 CTCCAG CTGGAG(16/14) 266 CTCCAG BsaJI 386 CCTGGG C^C[ACGT][ACGT]GG 415 CCCAGG Bsu36I 71 CCTCAGG CC^T[ACGT]AGG 178 CCTAAGG Cfr13I 413 GGCCC G^G[ACGT]CC 444 GGCCC DpnII 97 GATC ^GATC 373 GATC Eco81I 71 CCTCAGG CC^T[ACGT]AGG 178 CCTAAGG MboI 97 GATC ^GATC 373 GATC NdeII 97 GATC ^GATC 373 GATC PstI 328 CTGCAG CTGCA^G 350 CTGCAG Sau3AI 97 GATC ^GATC 373 GATC Sau96I 413 GGCCC G^G[ACGT]CC 444 GGCCC TfiI 63 GATTC G^A[AT]TC 450 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BbvI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboII MnlI MvaI MwoI ScrFI SfcI TseI TspRI