################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AgeI 123 ACCGGT A^CCGGT AlwNI 673 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 692 GAGCTC G[AT]GC[AT]^C AspI 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 420 CCCGGG C^[CT]CG[GA]G BamHI 362 GGATCC G^GATCC BanI 577 GGCACC G^G[CT][GA]CC BbrPI 517 CACGTG CAC^GTG BglI 569 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 493 CTCCAG CTGGAG(16/14) BsaAI 517 CACGTG [CT]AC^GT[GA] BsaWI 123 ACCGGT [AT]^CCGG[AT] BsiHKAI 692 GAGCTC G[AT]GC[AT]^C BsmAI 902 GAGAC GTCTC(1/5) BsoBI 420 CCCGGG C^[CT]CG[GA]G BspHI 854 TCATGA T^CATGA BstEII 720 GGTCACC G^GT[ACGT]ACC BstPI 720 GGTCACC G^GT[ACGT]ACC CfoI 347 GCGC GCG^C DdeI 178 CTCAG C^T[ACGT]AG DraIII 834 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 571 CGGCCG C^GGCCG EclXI 571 CGGCCG C^GGCCG Eco52I 571 CGGCCG C^GGCCG EcoO65I 720 GGTCACC G^GT[ACGT]ACC EcoT14I 32 CCATGG C^C[AT][AT]GG HaeII 346 AGCGCC [GA]GCGC^[CT] HhaI 347 GCGC GCG^C HinP1I 347 GCGC G^CGC MseI 959 TTAA T^TAA MspA1I 88 CAGCGG C[AC]G^C[GT]G NaeI 569 GCCGGC GCC^GGC NcoI 32 CCATGG C^CATGG NgoMIV 569 GCCGGC G^CCGGC PflFI 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 123 ACCGGT A^CCGGT PleI 937 GACTC GAGTC(4/5) PmaCI 517 CACGTG CAC^GTG PmlI 517 CACGTG CAC^GTG PpuMI 165 GGGACCC [GA]G^G[AT]CC[CT] PstI 553 CTGCAG CTGCA^G RcaI 854 TCATGA T^CATGA SacI 692 GAGCTC GAGCT^C SbfI 552 CCTGCAGG CCTGCA^GG SmaI 420 CCCGGG CCC^GGG SmlI 1021 CTCAAG C^T[CT][GA]AG Sse8387I 552 CCTGCAGG CCTGCA^GG StyI 32 CCATGG C^C[AT][AT]GG Tru9I 959 TTAA T^TAA Tsp45I 721 GTCAC ^GT[GC]AC Tsp509I 922 AATT ^AATT Tth111I 610 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 725 CCAGTAATGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 420 CCCGGG C^CCGGG XmaI 420 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BalI 254 TGGCCA TGG^CCA 917 TGGCCA BanII 692 GAGCTC G[GA]GC[CT]^C 804 GGGCTC BfaI 94 CTAG C^TAG 142 CTAG BseRI 400 GAGGAG GAGGAG(10/8) 526 GAGGAG BsiEI 571 CGGCCG CG[GA][CT]^CG 654 CGACCG BspMI 810 ACCTGC ACCTGC(4/8) 555 GCAGGT BstXI 628 CCAAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 908 CCATCTACCTGG EcoNI 477 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 666 CCTACAGCAGG MaeI 94 CTAG C^TAG 142 CTAG MluNI 254 TGGCCA TGG^CCA 917 TGGCCA MscI 254 TGGCCA TGG^CCA 917 TGGCCA NheI 93 GCTAGC G^CTAGC 141 GCTAGC SfaNI 15 GATGC GCATC(5/9) 294 GATGC TfiI 102 GATTC G^A[AT]TC 323 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TseI TspRI XhoII