(Circular) (Six-base) MAPSORT of: pFN21AE1230.seq Check: 9454 from: 1 to: 5734 pFN21AE1230 5734 bp With 182 enzymes: SgfI * April 28, 2014 11:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2551 2824 2551 Size: 273 5461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2308 3797 5181 5449 1632 Size: 676 1489 1384 268 1917 Fragments arranged by size: 1917 1489 1384 676 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 4633 5610 1203 Size: 3430 977 1327 Fragments arranged by size: 3430 1327 977 AgeI A'CCGG_T Cuts at: 2128 4565 2128 Size: 2437 3297 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AlwNI CAG_nnn'CTG Cuts at: 2683 3477 5049 2683 Size: 794 1572 3368 Fragments arranged by size: 3368 1572 794 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2425 3059 1870 Size: 73 482 634 4545 Fragments arranged by size: 4545 634 482 73 BamHI G'GATC_C Cuts at: 2367 3064 3431 2367 Size: 697 367 4670 Fragments arranged by size: 4670 697 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2582 3055 4510 618 Size: 325 217 627 795 473 1455 1842 Fragments arranged by size: 1842 1455 795 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2701 2813 3053 1391 Size: 1310 112 240 4072 Fragments arranged by size: 4072 1310 240 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3925 3959 1903 Size: 34 50 34 1904 34 3678 Fragments arranged by size: 3678 1904 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2580 4345 136 Size: 107 122 71 631 605 908 1765 1525 Fragments arranged by size: 1765 1525 908 631 605 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2585 3795 4951 1790 Size: 795 1210 1156 2573 Fragments arranged by size: 2573 1210 1156 795 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 3465 4423 Size: 416 53 83 399 24 760 1288 958 Cuts at: 4423 442 Size: 1753 Fragments arranged by size: 1753 1288 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2102 2150 1056 Size: 1046 48 4640 Fragments arranged by size: 4640 1046 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 4394 1239 Size: 1244 1911 2579 Fragments arranged by size: 2579 1911 1244 Bpu10I CC'TnA_GC Cuts at: 2183 2183 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3011 3859 4724 5022 3011 Size: 848 865 298 3723 Fragments arranged by size: 3723 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2524 3525 493 Size: 711 1320 1001 2702 Fragments arranged by size: 2702 1320 1001 711 BsaBI GATnn'nnATC Cuts at: 5728 5728 Size: 5734 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2128 4167 4565 4839 4986 5397 2128 Size: 2039 398 274 147 411 2465 Fragments arranged by size: 2465 2039 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 1178 Size: 30 165 30 1041 30 4438 Fragments arranged by size: 4438 1041 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2546 1186 Size: 1234 126 4374 Fragments arranged by size: 4374 1234 126 BseYI C'CCAG_C Cuts at: 2174 4937 5433 2174 Size: 2763 496 2475 Fragments arranged by size: 2763 2475 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 2330 2498 1163 Size: 573 417 177 168 4399 Fragments arranged by size: 4399 573 417 177 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2579 2662 3184 3945 4973 1540 Size: 275 186 578 83 522 761 1028 2301 Fragments arranged by size: 2301 1028 761 578 522 275 186 83 BsiHKAI G_wGCw'C Cuts at: 2701 3795 3880 4951 2701 Size: 1094 85 1071 3484 Fragments arranged by size: 3484 1094 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2585 2701 2813 3053 3795 3880 4951 Size: 399 795 116 112 240 742 85 1071 Cuts at: 4951 1391 Size: 2174 Fragments arranged by size: 2174 1071 795 742 399 240 116 112 85 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 2859 3623 5353 2859 Size: 764 1730 3240 Fragments arranged by size: 3240 1730 764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2551 2824 3089 844 Size: 1707 273 265 3489 Fragments arranged by size: 3489 1707 273 265 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2128 2338 2447 2574 2661 4378 4565 Size: 110 318 210 109 127 87 1717 187 Cuts at: 4565 1700 Size: 2869 Fragments arranged by size: 2869 1717 318 210 187 127 110 109 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 2725 1184 Size: 1541 4193 BstXI CCAn_nnnn'nTGG Cuts at: 2640 2920 2640 Size: 280 5454 BstYI r'GATC_y Cuts at: 1972 2367 2493 2880 3064 3431 3816 3833 5285 Size: 395 126 387 184 367 385 17 1452 Cuts at: 5285 5371 5383 5729 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 395 387 385 367 346 184 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2355 2773 513 Size: 552 972 318 418 3474 Fragments arranged by size: 3474 972 552 418 318 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2811 487 Size: 683 56 1585 3410 Fragments arranged by size: 3410 1585 683 56 BtsI GCAGTG_nn' Cuts at: 3250 4044 4064 3250 Size: 794 20 4920 Fragments arranged by size: 4920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2099 2134 529 Size: 35 1535 35 4129 Fragments arranged by size: 4129 1535 35 35 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DraIII CAC_nnn'GTG Cuts at: 2844 2844 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2358 2576 Size: 54 1425 50 275 18 429 99 218 Cuts at: 2576 2655 2922 3181 4070 5699 8 Size: 79 267 259 889 1629 43 Fragments arranged by size: 1629 1425 889 429 275 267 259 218 99 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2576 3181 1537 Size: 275 764 605 4090 Fragments arranged by size: 4090 764 605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3664 1353 Size: 2311 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2474 2919 4321 4695 4841 387 Size: 1064 138 885 445 1402 374 146 1280 Fragments arranged by size: 1402 1280 1064 885 445 374 146 138 EcoICRI GAG'CTC Cuts at: 2699 2699 Size: 5734 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2308 2483 3797 4394 5181 5449 1239 Size: 393 676 175 1314 597 787 268 1524 Fragments arranged by size: 1524 1314 787 676 597 393 268 175 EcoNI CCTnn'n_nnAGG Cuts at: 2486 2675 2486 Size: 189 5545 EcoO109I rG'GnC_Cy Cuts at: 2171 2554 2680 2171 Size: 383 126 5225 Fragments arranged by size: 5225 383 126 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2355 4881 1678 Size: 677 2526 2531 Fragments arranged by size: 2531 2526 677 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2898 2930 4375 4407 4449 Size: 32 135 32 1326 32 1445 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 1445 1326 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4848 5032 1305 Size: 3543 184 2007 Fragments arranged by size: 3543 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 2924 10 Size: 54 1425 343 429 663 2820 Fragments arranged by size: 2820 1425 663 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 2778 2858 3692 4051 4210 518 Size: 648 1554 58 80 834 359 159 2042 Fragments arranged by size: 2042 1554 834 648 359 159 80 58 MspA1I CmG'CkG Cuts at: 1606 2095 3150 3827 4975 5220 1606 Size: 489 1055 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 677 489 245 NaeI GCC'GGC Cuts at: 2576 2576 Size: 5734 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4210 Fragments arranged by size: 4210 972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NgoMIV G'CCGG_C Cuts at: 2574 2574 Size: 5734 NheI G'CTAG_C Cuts at: 1052 2098 2146 1052 Size: 1046 48 4640 Fragments arranged by size: 4640 1046 48 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 3092 4637 1132 Size: 97 1863 1545 2229 Fragments arranged by size: 2229 1863 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5734 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5734 PfoI T'CCnGG_A Cuts at: 2462 2499 2462 Size: 37 5697 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PmlI CAC'GTG Cuts at: 2524 2524 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3835 5316 5348 3803 Size: 32 1481 32 4189 Fragments arranged by size: 4189 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2171 2171 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PstI C_TGCA'G Cuts at: 830 2562 3086 830 Size: 1732 524 3478 Fragments arranged by size: 3478 1732 524 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 SacI G_AGCT'C Cuts at: 2701 2701 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SanDI GG'GwC_CC Cuts at: 2171 2171 Size: 5734 SbfI CC_TGCA'GG Cuts at: 2562 3086 2562 Size: 524 5210 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 2558 2672 2704 3082 4217 4898 5089 Size: 219 1513 114 32 378 1135 681 191 Cuts at: 5089 826 Size: 1471 Fragments arranged by size: 1513 1471 1135 681 378 219 191 114 32 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 2427 3061 1872 Size: 555 634 4545 Fragments arranged by size: 4545 634 555 SmlI C'TyrA_G Cuts at: 820 1017 1943 3026 3838 4739 5001 820 Size: 197 926 1083 812 901 262 1553 Fragments arranged by size: 1553 1083 926 901 812 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 3092 1132 Size: 1960 3774 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2037 3173 513 Size: 552 972 1136 3074 Fragments arranged by size: 3074 1136 972 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3980 5692 96 Size: 274 80 33 51 1443 2003 1712 138 Fragments arranged by size: 2003 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 2618 1316 Size: 1302 4432 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 2737 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5734 XmaI C'CCGG_G Cuts at: 1870 2425 3059 1870 Size: 555 634 4545 Fragments arranged by size: 4545 634 555 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI