################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 103 GTCTAC GT^[AC][GT]AC AciI 348 CCGC CCGC(-3/-1) AcsI 364 GAATTC [GA]^AATT[CT] AfaI 435 GTAC GT^AC ApoI 364 GAATTC [GA]^AATT[CT] AvaII 113 GGACC G^G[AT]CC BamHI 297 GGATCC G^GATCC BanI 208 GGTGCC G^G[CT][GA]CC BbrPI 292 CACGTG CAC^GTG BbsI 24 GAAGAC GAAGAC(2/6) BfaI 205 CTAG C^TAG BmyI 249 GGGCAC G[AGT]GC[ACT]^C BpuAI 24 GAAGAC GAAGAC(2/6) BsaAI 292 CACGTG [CT]AC^GT[GA] BseRI 19 GAGGAG GAGGAG(10/8) Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C BsrDI 323 GCAATG GCAATG(2/0) BsrI 96 ACTGG ACTGG(1/-1) BstF5I 63 GGATG GGATG(2/0) DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 364 GAATTC G^AATTC EcoT14I 109 CCTTGG C^C[AT][AT]GG FauI 347 CCCGC CCCGC(4/6) FokI 63 GGATG GGATG(9/13) MaeI 205 CTAG C^TAG MaeIII 281 GTGAC ^GT[ACGT]AC MboII 24 GAAGA GAAGA(8/7) MspA1I 262 CAGCTG C[AC]G^C[GT]G NlaIII 190 CATG CATG^ PmaCI 292 CACGTG CAC^GTG PmlI 292 CACGTG CAC^GTG PshAI 27 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 262 CAGCTG CAG^CTG RsaI 435 GTAC GT^AC SmlI 244 CTCAAG C^T[CT][GA]AG StyI 109 CCTTGG C^C[AT][AT]GG Tsp45I 281 GTGAC ^GT[GC]AC Tsp509I 365 AATT ^AATT TspRI 277 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AocI 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG BsgI 274 CTGCAC GTGCAG(16/14) 289 CTGCAC BstYI 141 GGATCT [GA]^GATC[CT] 297 GGATCC Bsu36I 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG DpnII 142 GATC ^GATC 298 GATC Eco81I 16 CCTGAGG CC^T[ACGT]AGG 175 CCTAAGG HinfI 12 GACTC G^A[ACGT]TC 130 GAGTC MaeII 59 ACGT A^CGT 293 ACGT MboI 142 GATC ^GATC 298 GATC MflI 141 GGATCT [GA]^GATC[CT] 297 GGATCC NdeII 142 GATC ^GATC 298 GATC PleI 12 GACTC GAGTC(4/5) 130 GAGTC Sau3AI 142 GATC ^GATC 298 GATC XhoII 141 GGATCT [GA]^GATC[CT] 297 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI AlwI BbvI BsaJI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HphI ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI SfaNI TseI