################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SmlI SnaBI Sse8387I SspBI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1174 GTCTAC GT^[AC][GT]AC AcyI 30 GGCGCC G[GA]^CG[CT]C AflIII 223 ACATGT A^C[GA][CT]GT Alw44I 380 GTGCAC G^TGCAC ApaI 561 GGGCCC GGGCC^C ApaLI 380 GTGCAC G^TGCAC BalI 524 TGGCCA TGG^CCA BamHI 1582 GGATCC G^GATCC BanII 561 GGGCCC G[GA]GC[CT]^C BbeI 30 GGCGCC GGCGC^C BclI 573 TGATCA T^GATCA BglII 1002 AGATCT A^GATCT BsaHI 30 GGCGCC G[GA]^CG[CT]C BsgI 1366 GTGCAG GTGCAG(16/14) BsmBI 1358 GAGACG CGTCTC(1/5) BspLU11I 223 ACATGT A^CATGT BspMI 589 GCAGGT ACCTGC(4/8) BsrFI 33 GCCGGT [GA]^CCGG[CT] BssSI 621 CACGAG CACGAG(-5/-1) BstXI 1524 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 31 GCGC GCG^C Cfr10I 33 GCCGGT [GA]^CCGG[CT] DraIII 1556 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 524 TGGCCA [CT]^GGCC[GA] EcoNI 1341 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 603 CCCGC CCCGC(4/6) FbaI 573 TGATCA T^GATCA HaeII 30 GGCGCC [GA]GCGC^[CT] HapII 34 CCGG C^CGG HgaI 1360 GACGC GACGC(5/10) HhaI 31 GCGC GCG^C Hin1I 30 GGCGCC G[GA]^CG[CT]C HinP1I 31 GCGC G^CGC HincII 127 GTCAAC GT[CT]^[GA]AC HindII 127 GTCAAC GT[CT]^[GA]AC HpaII 34 CCGG C^CGG KasI 30 GGCGCC G^GCGCC MluNI 524 TGGCCA TGG^CCA MscI 524 TGGCCA TGG^CCA MspI 34 CCGG C^CGG NarI 30 GGCGCC GG^CGCC PflMI 11 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1535 GAGTC GAGTC(4/5) PpuMI 1021 GGGACCC [GA]G^G[AT]CC[CT] PshAI 229 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 30 GGCGCC GGC^GCC SgrAI 32 CGCCGGTG C[GA]^CCGG[CT]G SpeI 1416 ACTAGT A^CTAGT SphI 879 GCATGC GCATG^C SspI 786 AATATT AAT^ATT StuI 1590 AGGCCT AGG^CCT Van91I 11 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 30 GGCGCC G^G[CT][GA]CC 1159 GGTGCC BsaI 440 GAGACC GGTCTC(1/5) 1605 GGTCTC BsrDI 419 GCAATG GCAATG(2/0) 725 GCAATG BstYI 1002 AGATCT [GA]^GATC[CT] 1582 GGATCC DraI 772 TTTAAA TTT^AAA 1147 TTTAAA DrdI 310 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1264 GACTTGGTGGTC EarI 1006 CTCTTC CTCTTC(1/4) 1168 GAAGAG EcoRI 6 GAATTC G^AATTC 682 GAATTC HphI 94 GGTGA GGTGA(8/7) 1304 TCACC Ksp632I 1006 CTCTTC CTCTTC(1/4) 1168 GAAGAG MflI 1002 AGATCT [GA]^GATC[CT] 1582 GGATCC MslI 1126 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1251 CACCATGGTG NspI 223 ACATGT [GA]CATG^[CT] 879 GCATGC SfcI 462 CTGTAG C^T[GA][CT]AG 1196 CTGTAG TaqI 320 TCGA T^CGA 819 TCGA TfiI 703 GAATC G^A[AT]TC 851 GATTC TthHB8I 320 TCGA T^CGA 819 TCGA XhoII 1002 AGATCT [GA]^GATC[CT] 1582 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI