Restriction map for
pFN21AE5572
(Circular) (Six-base) MAPSORT of: pFN21AE5572.seq Check: 9257 from: 1 to: 6088
pFN21AE5572 6088 bp
With 182 enzymes: SgfI *
May 22, 2017 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2831 2831
Size: 6088
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2640 3095 3276 4151 5535 5803 1632
Size: 1008 455 181 875 1384 268 1917
Fragments arranged by size:
1917 1384 1008 875 455 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2402 3146 3183 4987 5964 1203
Size: 1199 744 37 1804 977 1327
Fragments arranged by size:
1804 1327 1199 977 744 37
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 3831 5403 3831
Size: 1572 4516
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2121 2261 2611 3044 3397 3623 3775 5884
Size: 628 140 350 433 353 226 152 2109
Cuts at: 5884 1493
Size: 1697
Fragments arranged by size:
2109 1697 628 433 353 350 226 152
140
AseI AT'TA_AT
Cuts at: 160 1032 2340 2931 4642 160
Size: 872 1308 591 1711 1606
Fragments arranged by size:
1711 1606 1308 872 591
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2679 3413 1870
Size: 73 736 734 4545
Fragments arranged by size:
4545 736 734 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3327 3360 3327
Size: 33 6055
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3409 4864 618
Size: 325 217 627 1622 1455 1842
Fragments arranged by size:
1842 1622 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3073 3143 928
Size: 971 1174 70 3873
Fragments arranged by size:
3873 1174 971 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4279 4313 1903
Size: 34 50 34 2258 34 3678
Fragments arranged by size:
3678 2258 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3355 3980 5190 3355
Size: 625 1210 4253
Fragments arranged by size:
4253 1210 625
BclI T'GATC_A
Cuts at: 1548 2022 2101 1548
Size: 474 79 5535
Fragments arranged by size:
5535 474 79
BfrBI ATG'CAT
Cuts at: 2753 2753
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4748 1239
Size: 3509 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2204 4213 5078 5376 2204
Size: 2009 865 298 2916
Fragments arranged by size:
2916 2009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 2286 6082 2286
Size: 3796 2292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5863
Fragments arranged by size:
5863 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2873 1186
Size: 1687 4401
BseYI C'CCAG_C
Cuts at: 3069 3294 5291 5787 3069
Size: 225 1997 496 3370
Fragments arranged by size:
3370 1997 496 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4149 4234 5305 4149
Size: 85 1071 4932
Fragments arranged by size:
4932 1071 85
BsmI GAATG_Cn'
Cuts at: 2753 3599 3692 2753
Size: 846 93 5149
Fragments arranged by size:
5149 846 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3407 4149 4234 5305 1391
Size: 399 1617 742 85 1071 2174
Fragments arranged by size:
2174 1617 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2104 3977 5707 2104
Size: 1873 1730 2485
Fragments arranged by size:
2485 1873 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2716 2831 3443 844
Size: 1872 115 612 3489
Fragments arranged by size:
3489 1872 612 115
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 3009 3318 4583 4757 65
Size: 1183 1701 60 309 1265 174 1396
Fragments arranged by size:
1701 1396 1265 1183 309 174 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2087 96
Size: 1991 4097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2226 4148 5160 2226
Size: 1922 1012 3154
Fragments arranged by size:
3154 1922 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 2896 4892 2896
Size: 1996 4092
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 3050 3418 3785 4170 4187 5639 5725 5737
Size: 1078 368 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1078 385 368 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5536
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 3604 4398 4418 2529
Size: 1075 794 20 4199
Fragments arranged by size:
4199 1075 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 2313 3392 2313
Size: 1079 5009
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 3535 4424
Size: 54 1425 50 275 18 302 1403 889
Cuts at: 4424 6053 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1403 889 302 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2536 3070 3297 4018 1353
Size: 804 379 534 227 721 3423
Fragments arranged by size:
3423 804 721 534 379 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2640 3095 3276 4151 4748 5535 5803
Size: 393 1008 455 181 875 597 787 268
Cuts at: 5803 1239
Size: 1524
Fragments arranged by size:
1524 1008 875 787 597 455 393 268
181
EcoO109I rG'GnC_Cy
Cuts at: 3242 3242
Size: 6088
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
EcoRV GAT'ATC
Cuts at: 2069 2486 2069
Size: 417 5671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2198 2198
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2754 2786 4729
Size: 32 135 32 486 32 664 32 1943
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1943 664 486 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3136 3358 3432 3678 669
Size: 988 281 1198 222 74 246 3079
Fragments arranged by size:
3079 1198 988 281 246 222 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5202 5386 1305
Size: 3897 184 2007
Fragments arranged by size:
3897 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2533 4046 4405 4564 518
Size: 648 858 509 1513 359 159 2042
Fragments arranged by size:
2042 1513 858 648 509 359 159
MspA1I CmG'CkG
Cuts at: 1606 3504 4181 5329 5574 1606
Size: 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5536
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NsiI A_TGCA'T
Cuts at: 2755 2755
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2406 3150 3187 3446 4991 1132
Size: 97 1177 744 37 259 1545 2229
Fragments arranged by size:
2229 1545 1177 744 259 97 37
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 2402 3146 3183 4987 2402
Size: 744 37 1804 3503
Fragments arranged by size:
3503 1804 744 37
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3242 3242
Size: 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 3242 3242
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 2707 2707
Size: 6088
SfcI C'TryA_G
Cuts at: 826 1045 3436 4571 5252 5443 826
Size: 219 2391 1135 681 191 1471
Fragments arranged by size:
2391 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 4192 5093 5355 820
Size: 197 926 240 2009 901 262 1553
Fragments arranged by size:
2009 1553 926 901 262 240 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 2854 4012 5
Size: 47 2802 1158 2081
Fragments arranged by size:
2802 2081 1158 47
StuI AGG'CCT
Cuts at: 1517 2043 1517
Size: 526 5562
StyI C'CwwG_G
Cuts at: 513 1065 2720 3339 3527 513
Size: 552 1655 619 188 3074
Fragments arranged by size:
3074 1655 619 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 3153 4115 4132 4285 4470 4915
Size: 1255 1092 481 962 17 153 185 445
Cuts at: 4915 325
Size: 1498
Fragments arranged by size:
1498 1255 1092 962 481 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2087 2474 2937
Size: 274 80 33 51 1443 110 387 463
Cuts at: 2937 4334 6046 96
Size: 1397 1712 138
Fragments arranged by size:
1712 1443 1397 463 387 274 138 110
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2192 2224 6048 6080 2192
Size: 32 3824 32 2200
Fragments arranged by size:
3824 2200 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 2545 3035 4217 2545
Size: 490 1182 4416
Fragments arranged by size:
4416 1182 490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI XcmI