################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstEII BstPI BstUI Bsu36I CelII CfoI ClaI CpoI DraII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Alw44I 1519 GTGCAC G^TGCAC ApaLI 1519 GTGCAC G^TGCAC BbvCI 2254 CCTCAGC CCTCAGC(-5/-2) BcgI 622 GCAGATTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2591 GTATCC GTATCC(6/5) BpmI 2015 CTGGAG CTGGAG(16/14) BsaAI 17 TACGTG [CT]AC^GT[GA] BseRI 2719 GAGGAG GAGGAG(10/8) BsmFI 451 GGGAC GGGAC(10/14) BsrFI 2471 ACCGGC [GA]^CCGG[CT] Bst1107I 1921 GTATAC GTA^TAC BstAPI 2259 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1727 TTCGAA TT^CGAA BstZ17I 1921 GTATAC GTA^TAC Cfr10I 2471 ACCGGC [GA]^CCGG[CT] Cfr13I 2601 GGCCC G^G[ACGT]CC DraIII 828 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 2727 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 2580 GCGGG CCCGC(4/6) HapII 2472 CCGG C^CGG HincII 2059 GTTGAC GT[CT]^[GA]AC HindII 2059 GTTGAC GT[CT]^[GA]AC HpaII 2472 CCGG C^CGG MspA1I 1109 CAGCTG C[AC]G^C[GT]G MspI 2472 CCGG C^CGG NspI 2648 ACATGC [GA]CATG^[CT] NspV 1727 TTCGAA TT^CGAA PflMI 1798 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1112 CTGCAG CTGCA^G PvuII 1109 CAGCTG CAG^CTG SapI 1468 GAAGAGC GCTCTTC(1/4) Sau96I 2601 GGCCC G^G[ACGT]CC SexAI 333 ACCTGGT A^CC[AT]GGT SfuI 1727 TTCGAA TT^CGAA Van91I 1798 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1781 CCAAAGATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 1440 GTCTAC GT^[AC][GT]AC 1921 GTATAC AciI 506 GCGG CCGC(-3/-1) 2580 GCGG AfaI 1881 GTAC GT^AC 2634 GTAC AlwI 1270 GATCC GGATC(4/5) 1807 GGATC AspHI 1498 GAGCAC G[AT]GC[AT]^C 1519 GTGCAC BanI 1660 GGCACC G^G[CT][GA]CC 2290 GGCACC BglII 2555 AGATCT A^GATCT 2660 AGATCT BsaI 330 GAGACC GGTCTC(1/5) 2722 GAGACC BsiHKAI 1498 GAGCAC G[AT]GC[AT]^C 1519 GTGCAC BspHI 719 TCATGA T^CATGA 1149 TCATGA BspMI 209 ACCTGC ACCTGC(4/8) 2025 ACCTGC BstXI 1016 CCATGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2007 CCACAAAGCTGG BstYI 2555 AGATCT [GA]^GATC[CT] 2660 AGATCT DrdI 1606 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2505 GACTCTAAAGTC EcoRV 54 GATATC GAT^ATC 1786 GATATC EcoT22I 714 ATGCAT ATGCA^T 2650 ATGCAT HindIII 1346 AAGCTT A^AGCTT 2274 AAGCTT HphI 207 TCACC GGTGA(8/7) 1557 TCACC MaeII 18 ACGT A^CGT 1975 ACGT MfeI 277 CAATTG C^AATTG 661 CAATTG MflI 2555 AGATCT [GA]^GATC[CT] 2660 AGATCT MslI 1126 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1648 CATTGCAATG MunI 277 CAATTG C^AATTG 661 CAATTG NcoI 985 CCATGG C^CATGG 1125 CCATGG NsiI 714 ATGCAT ATGCA^T 2650 ATGCAT PshAI 453 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 751 GACATTGGTC RcaI 719 TCATGA T^CATGA 1149 TCATGA RsaI 1881 GTAC GT^AC 2634 GTAC SspI 163 AATATT AAT^ATT 1628 AATATT Tsp45I 818 GTGAC ^GT[GC]AC 2225 GTGAC XhoII 2555 AGATCT [GA]^GATC[CT] 2660 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AcsI AluI AlwNI ApoI Asp700I BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI DsaI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI