(Circular) (Six-base) MAPSORT of: pFN21AE3322.seq Check: 3505 from: 1 to: 7444 pFN21AE3322 7444 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2223 2223 Size: 7444 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5254 278 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3446 3927 4787 1937 Size: 1509 481 860 4594 Fragments arranged by size: 4594 1509 860 481 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 4718 5507 6891 7159 1632 Size: 821 2265 789 1384 268 1917 Fragments arranged by size: 2265 1917 1384 821 789 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 6343 7320 1203 Size: 5140 977 1327 Fragments arranged by size: 5140 1327 977 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3357 1184 Size: 2173 5271 AlwNI CAG_nnn'CTG Cuts at: 2276 3116 4270 5187 6759 2276 Size: 840 1154 917 1572 2961 Fragments arranged by size: 2961 1572 1154 917 840 ApaLI G'TGCA_C Cuts at: 3524 5501 6657 3524 Size: 1977 1156 4311 Fragments arranged by size: 4311 1977 1156 ApoI r'AATT_y Cuts at: 1493 2582 3000 3233 3898 4185 4360 4753 4979 Size: 1089 418 233 665 287 175 393 226 Cuts at: 4979 5131 7240 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1089 665 418 393 287 233 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 5998 160 Size: 872 4966 1606 Fragments arranged by size: 4966 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 BamHI G'GATC_C Cuts at: 4774 5141 4774 Size: 367 7077 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3665 4295 4765 6220 618 Size: 325 217 627 1878 630 470 1455 1842 Fragments arranged by size: 1878 1842 1455 630 627 470 325 217 BanII G_rGCy'C Cuts at: 1391 4763 1391 Size: 3372 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2756 3639 3877 4514 928 Size: 971 857 883 238 637 3858 Fragments arranged by size: 3858 971 883 857 637 238 BbvCI CC'TCA_GC Cuts at: 4260 4260 Size: 7444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2616 2650 5635 5669 1903 Size: 34 679 34 2985 34 3678 Fragments arranged by size: 3678 2985 679 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5336 6546 4607 Size: 729 1210 5505 Fragments arranged by size: 5505 1210 729 BclI T'GATC_A Cuts at: 1548 1548 Size: 7444 BfrBI ATG'CAT Cuts at: 2721 4657 2721 Size: 1936 5508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6055 136 Size: 107 122 71 631 605 4383 1525 Fragments arranged by size: 4383 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4560 4665 7439 4560 Size: 105 2774 4565 Fragments arranged by size: 4565 2774 105 BlpI GC'TnA_GC Cuts at: 4861 4861 Size: 7444 Bme1580I G_kGCm'C Cuts at: 1790 3528 4083 4308 5505 6661 1790 Size: 1738 555 225 1197 1156 2573 Fragments arranged by size: 2573 1738 1197 1156 555 225 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 4385 4644 Size: 416 53 83 399 24 647 2321 259 Cuts at: 4644 5175 6133 442 Size: 531 958 1753 Fragments arranged by size: 2321 1753 958 647 531 416 399 259 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4041 6104 1239 Size: 2802 2063 2579 Fragments arranged by size: 2802 2579 2063 Bpu10I CC'TnA_GC Cuts at: 4260 4260 Size: 7444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3356 3500 3706 5569 6434 6732 3356 Size: 144 206 1863 865 298 4068 Fragments arranged by size: 4068 1863 865 298 206 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2329 4721 6107 882 Size: 617 830 2392 1386 2219 Fragments arranged by size: 2392 2219 1386 830 617 BsaAI yAC'GTr Cuts at: 493 1204 2024 5235 493 Size: 711 820 3211 2702 Fragments arranged by size: 3211 2702 820 711 BsaBI GATnn'nnATC Cuts at: 7438 7438 Size: 7444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5251 5633 6268 275 Size: 53 83 186 936 3718 382 635 1451 Fragments arranged by size: 3718 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5877 6275 6549 6696 7107 5877 Size: 398 274 147 411 6214 Fragments arranged by size: 6214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7219 Fragments arranged by size: 7219 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4739 1186 Size: 3553 3891 BseYI C'CCAG_C Cuts at: 4701 6647 7143 4701 Size: 1946 496 5002 Fragments arranged by size: 5002 1946 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6871 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3507 3528 5505 5590 6661 3507 Size: 21 1977 85 1071 4290 Fragments arranged by size: 4290 1977 1071 85 21 BsmI GAATG_Cn' Cuts at: 2078 2556 2929 3944 4702 4955 5048 2078 Size: 478 373 1015 758 253 93 4474 Fragments arranged by size: 4474 1015 758 478 373 253 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3507 3528 4083 4308 4763 5505 5590 Size: 399 1717 21 555 225 455 742 85 Cuts at: 5590 6661 1391 Size: 1071 2174 Fragments arranged by size: 2174 1717 1071 742 555 455 399 225 85 21 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 2724 3154 5333 7063 2724 Size: 430 2179 1730 3105 Fragments arranged by size: 3105 2179 1730 430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 4039 4799 844 Size: 1379 1816 760 3489 Fragments arranged by size: 3489 1816 1379 760 BsrBI CCG'CTC Cuts at: 1394 1823 5331 1394 Size: 429 3508 3507 Fragments arranged by size: 3508 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3142 3339 3652 3664 5939 6113 65 Size: 1183 1894 197 313 12 2275 174 1396 Fragments arranged by size: 2275 1894 1396 1183 313 197 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 4476 6088 6275 1700 Size: 110 2666 1612 187 2869 Fragments arranged by size: 2869 2666 1612 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7444 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 4270 794 Size: 3476 3968 BstBI TT'CG_AA Cuts at: 3733 6248 3733 Size: 2515 4929 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7444 BstXI CCAn_nnnn'nTGG Cuts at: 3028 4019 3028 Size: 991 6453 BstYI r'GATC_y Cuts at: 1972 4560 4665 4774 5141 5526 5543 6995 7081 Size: 2588 105 109 367 385 17 1452 86 Cuts at: 7081 7093 7439 1972 Size: 12 346 1977 Fragments arranged by size: 2588 1977 1452 385 367 346 109 105 86 17 12 BstZ17I GTA'TAC Cuts at: 3928 3928 Size: 7444 BtgI C'CryG_G Cuts at: 513 1065 2004 2887 2990 3130 513 Size: 552 939 883 103 140 4827 Fragments arranged by size: 4827 939 883 552 140 103 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2934 4232 4268 4960 5754 5774 2097 Size: 837 1298 36 692 794 20 3767 Fragments arranged by size: 3767 1298 837 794 692 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 3049 3639 3856 4748 3049 Size: 590 217 892 5745 Fragments arranged by size: 5745 892 590 217 DraIII CAC_nnn'GTG Cuts at: 2838 2838 Size: 7444 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3617 4516 6451 809 Size: 507 2301 899 1935 1802 Fragments arranged by size: 2301 1935 1802 899 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4891 5780 7409 Size: 54 1425 50 275 18 3061 889 1629 Cuts at: 7409 8 Size: 43 Fragments arranged by size: 3061 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2682 3468 3684 4668 4686 5374 1353 Size: 1181 148 786 216 984 18 688 3423 Fragments arranged by size: 3423 1181 984 786 688 216 148 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6031 6405 6551 387 Size: 1064 138 4442 374 146 1280 Fragments arranged by size: 4442 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 4041 4718 5507 6104 6891 7159 Size: 393 821 1588 677 789 597 787 268 Cuts at: 7159 1239 Size: 1524 Fragments arranged by size: 1588 1524 821 789 787 677 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4736 4736 Size: 7444 EcoRI G'AATT_C Cuts at: 2582 4185 4360 4753 5131 2582 Size: 1603 175 393 378 4895 Fragments arranged by size: 4895 1603 393 378 175 EcoRV GAT'ATC Cuts at: 2061 3793 2061 Size: 1732 5712 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 7444 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 6591 1678 Size: 4913 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 3809 3841 3905 Size: 32 135 32 830 32 1375 32 64 Cuts at: 3905 3937 4132 4164 6085 6117 6159 6191 1373 Size: 32 195 32 1921 32 42 32 2626 Fragments arranged by size: 2626 1921 1375 830 195 135 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4066 4788 5034 669 Size: 988 281 2128 722 246 3079 Fragments arranged by size: 3079 2128 988 722 281 246 HindIII A'AGCT_T Cuts at: 748 3351 4279 748 Size: 2603 928 3913 Fragments arranged by size: 3913 2603 928 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 2282 2666 5043 2282 Size: 384 2377 4683 Fragments arranged by size: 4683 2377 384 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3499 6558 6742 1305 Size: 2194 3059 184 2007 Fragments arranged by size: 3059 2194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5622 Fragments arranged by size: 5622 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3135 3657 5402 5761 5920 518 Size: 648 1969 522 1745 359 159 2042 Fragments arranged by size: 2042 1969 1745 648 522 359 159 MspA1I CmG'CkG Cuts at: 1606 3116 4860 5537 6685 6930 1606 Size: 1510 1744 677 1148 245 2120 Fragments arranged by size: 2120 1744 1510 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2990 3130 513 Size: 552 939 986 140 4827 Fragments arranged by size: 4827 986 939 552 140 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NsiI A_TGCA'T Cuts at: 2723 4659 2723 Size: 1936 5508 NspI r_CATG'y Cuts at: 1132 1229 4657 4802 6347 1132 Size: 97 3428 145 1545 2229 Fragments arranged by size: 3428 2229 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 4300 1348 Size: 2952 4492 PciI A'CATG_T Cuts at: 6343 6343 Size: 7444 PflMI CCAn_nnn'nTGG Cuts at: 1108 3809 1108 Size: 2701 4743 PfoI T'CCnGG_A Cuts at: 4599 4599 Size: 7444 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2462 2760 1613 Size: 849 298 6297 Fragments arranged by size: 6297 849 298 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PstI C_TGCA'G Cuts at: 830 3121 4796 830 Size: 2291 1675 3478 Fragments arranged by size: 3478 2291 1675 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 3116 3116 Size: 7444 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SapI GCTCTTCn'nnn_ Cuts at: 3468 3468 Size: 7444 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 5692 7404 5692 Size: 1712 5732 SexAI A'CCwGG_T Cuts at: 2338 2338 Size: 7444 SfcI C'TryA_G Cuts at: 826 1045 2194 2604 3117 3574 4343 4792 5927 Size: 219 1149 410 513 457 769 449 1135 Cuts at: 5927 6608 6799 826 Size: 681 191 1471 Fragments arranged by size: 1471 1149 1135 769 681 513 457 449 410 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3371 3479 3685 5548 6449 6711 Size: 197 926 1428 108 206 1863 901 262 Cuts at: 6711 820 Size: 1553 Fragments arranged by size: 1863 1553 1428 926 901 262 206 197 108 SnaBI TAC'GTA Cuts at: 493 5235 493 Size: 4742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 2170 3635 5368 5 Size: 47 2118 1465 1733 2081 Fragments arranged by size: 2118 2081 1733 1465 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7444 StyI C'CwwG_G Cuts at: 513 1065 2004 2622 2990 3130 3889 4434 4883 Size: 552 939 618 368 140 759 545 449 Cuts at: 4883 513 Size: 3074 Fragments arranged by size: 3074 939 759 618 552 545 449 368 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4313 5471 5488 5641 5826 6271 325 Size: 1255 2733 1158 17 153 185 445 1498 Fragments arranged by size: 2733 1498 1255 1158 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5690 7402 96 Size: 274 80 33 51 1443 3713 1712 138 Fragments arranged by size: 3713 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2725 2757 3497 3529 7404 7436 2725 Size: 32 740 32 3875 32 2733 Fragments arranged by size: 3875 2733 740 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3793 3793 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 2291 2868 3898 5573 2291 Size: 577 1030 1675 4162 Fragments arranged by size: 4162 1675 1030 577 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5252 276 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI