(Linear) (Six-base) MAPSORT of: KIEE3322.seq Check: 9943 from: 1 to: 2739 KIEE3322.orf With 182 enzymes: SgfI * December 5, 2014 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 218 2739 Size: 218 2521 AccI GT'mk_AC Cuts at: 0 1441 1922 2739 Size: 1441 481 817 Fragments arranged by size: 1441 817 481 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 2713 2739 Size: 448 2265 26 Fragments arranged by size: 2265 448 26 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1352 2739 Size: 1352 1387 AlwNI CAG_nnn'CTG Cuts at: 0 271 1111 2265 2739 Size: 271 840 1154 474 Fragments arranged by size: 1154 840 474 271 ApaLI G'TGCA_C Cuts at: 0 1519 2739 Size: 1519 1220 ApoI r'AATT_y Cuts at: 0 577 995 1228 1893 2180 2355 2739 Size: 577 418 233 665 287 175 384 Fragments arranged by size: 665 577 418 384 287 233 175 BanI G'GyrC_C Cuts at: 0 1660 2290 2739 Size: 1660 630 449 Fragments arranged by size: 1660 630 449 BbsI GAAGACnn'nnnn_ Cuts at: 0 751 1634 1872 2509 2739 Size: 751 883 238 637 230 Fragments arranged by size: 883 751 637 238 230 BbvCI CC'TCA_GC Cuts at: 0 2255 2739 Size: 2255 484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 611 645 2739 Size: 611 34 2094 Fragments arranged by size: 2094 611 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2602 2739 Size: 2602 137 BfrBI ATG'CAT Cuts at: 0 716 2652 2739 Size: 716 1936 87 Fragments arranged by size: 1936 716 87 BglII A'GATC_T Cuts at: 0 2555 2660 2739 Size: 2555 105 79 Fragments arranged by size: 2555 105 79 Bme1580I G_kGCm'C Cuts at: 0 1523 2078 2303 2739 Size: 1523 555 225 436 Fragments arranged by size: 1523 555 436 225 BmrI ACTGGGnnnn_n' Cuts at: 0 59 2380 2639 2739 Size: 59 2321 259 100 Fragments arranged by size: 2321 259 100 59 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2036 2739 Size: 2036 703 Bpu10I CC'TnA_GC Cuts at: 0 2255 2739 Size: 2255 484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1351 1495 1701 2739 Size: 1351 144 206 1038 Fragments arranged by size: 1351 1038 206 144 BsaI GGTCTCn'nnnn_ Cuts at: 0 324 2716 2739 Size: 324 2392 23 Fragments arranged by size: 2392 324 23 BsaAI yAC'GTr Cuts at: 0 19 2739 Size: 19 2720 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2734 2739 Size: 2734 5 BseYI C'CCAG_C Cuts at: 0 2696 2739 Size: 2696 43 BsiHKAI G_wGCw'C Cuts at: 0 1502 1523 2739 Size: 1502 21 1216 Fragments arranged by size: 1502 1216 21 BsmI GAATG_Cn' Cuts at: 0 73 551 924 1939 2697 2739 Size: 73 478 373 1015 758 42 Fragments arranged by size: 1015 758 478 373 73 42 Bsp1286I G_dGCh'C Cuts at: 0 1502 1523 2078 2303 2739 Size: 1502 21 555 225 436 Fragments arranged by size: 1502 555 436 225 21 BspHI T'CATG_A Cuts at: 0 719 1149 2739 Size: 719 430 1590 Fragments arranged by size: 1590 719 430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 218 2034 2739 Size: 218 1816 705 Fragments arranged by size: 1816 705 218 BsrDI GCAATG_nn' Cuts at: 0 1137 1334 1647 1659 2739 Size: 1137 197 313 12 1080 Fragments arranged by size: 1137 1080 313 197 12 BsrFI r'CCGG_y Cuts at: 0 2471 2739 Size: 2471 268 BstAPI GCAn_nnn'nTGC Cuts at: 0 2265 2739 Size: 2265 474 BstBI TT'CG_AA Cuts at: 0 1728 2739 Size: 1728 1011 BstXI CCAn_nnnn'nTGG Cuts at: 0 1023 2014 2739 Size: 1023 991 725 Fragments arranged by size: 1023 991 725 BstYI r'GATC_y Cuts at: 0 2555 2660 2739 Size: 2555 105 79 Fragments arranged by size: 2555 105 79 BstZ17I GTA'TAC Cuts at: 0 1923 2739 Size: 1923 816 BtgI C'CryG_G Cuts at: 0 882 985 1125 2739 Size: 882 103 140 1614 Fragments arranged by size: 1614 882 140 103 BtsI GCAGTG_nn' Cuts at: 0 92 929 2227 2263 2739 Size: 92 837 1298 36 476 Fragments arranged by size: 1298 837 476 92 36 DraI TTT'AAA Cuts at: 0 1044 1634 1851 2739 Size: 1044 590 217 888 Fragments arranged by size: 1044 888 590 217 DraIII CAC_nnn'GTG Cuts at: 0 833 2739 Size: 833 1906 DrdI GACnn_nn'nnGTC Cuts at: 0 1612 2511 2739 Size: 1612 899 228 Fragments arranged by size: 1612 899 228 EarI CTCTTCn'nnn_ Cuts at: 0 529 677 1463 1679 2663 2681 2739 Size: 529 148 786 216 984 18 58 Fragments arranged by size: 984 786 529 216 148 58 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 2036 2713 2739 Size: 448 1588 677 26 Fragments arranged by size: 1588 677 448 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 2731 2739 Size: 2731 8 EcoRI G'AATT_C Cuts at: 0 577 2180 2355 2739 Size: 577 1603 175 384 Fragments arranged by size: 1603 577 384 175 EcoRV GAT'ATC Cuts at: 0 56 1788 2739 Size: 56 1732 951 Fragments arranged by size: 1732 951 56 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 306 2739 Size: 306 2433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 397 429 1804 1836 1900 1932 2127 2159 Size: 397 32 1375 32 64 32 195 32 Cuts at: 2159 2739 Size: 580 Fragments arranged by size: 1375 580 397 195 64 32 32 32 32 HincII GTy'rAC Cuts at: 0 2061 2739 Size: 2061 678 HindIII A'AGCT_T Cuts at: 0 1346 2274 2739 Size: 1346 928 465 Fragments arranged by size: 1346 928 465 MfeI C'AATT_G Cuts at: 0 277 661 2739 Size: 277 384 2078 Fragments arranged by size: 2078 384 277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1494 2739 Size: 1494 1245 MslI CAynn'nnrTG Cuts at: 0 1130 1652 2739 Size: 1130 522 1087 Fragments arranged by size: 1130 1087 522 MspA1I CmG'CkG Cuts at: 0 1111 2739 Size: 1111 1628 NcoI C'CATG_G Cuts at: 0 985 1125 2739 Size: 985 140 1614 Fragments arranged by size: 1614 985 140 NsiI A_TGCA'T Cuts at: 0 718 2654 2739 Size: 718 1936 85 Fragments arranged by size: 1936 718 85 NspI r_CATG'y Cuts at: 0 2652 2739 Size: 2652 87 PasI CC'CwG_GG Cuts at: 0 2295 2739 Size: 2295 444 PflMI CCAn_nnn'nTGG Cuts at: 0 1804 2739 Size: 1804 935 PfoI T'CCnGG_A Cuts at: 0 2594 2739 Size: 2594 145 PshAI GACnn'nnGTC Cuts at: 0 457 755 2739 Size: 457 298 1984 Fragments arranged by size: 1984 457 298 PstI C_TGCA'G Cuts at: 0 1116 2739 Size: 1116 1623 PvuII CAG'CTG Cuts at: 0 1111 2739 Size: 1111 1628 SapI GCTCTTCn'nnn_ Cuts at: 0 1463 2739 Size: 1463 1276 SexAI A'CCwGG_T Cuts at: 0 333 2739 Size: 333 2406 SfcI C'TryA_G Cuts at: 0 189 599 1112 1569 2338 2739 Size: 189 410 513 457 769 401 Fragments arranged by size: 769 513 457 410 401 189 SmlI C'TyrA_G Cuts at: 0 1366 1474 1680 2739 Size: 1366 108 206 1059 Fragments arranged by size: 1366 1059 206 108 SspI AAT'ATT Cuts at: 0 165 1630 2739 Size: 165 1465 1109 Fragments arranged by size: 1465 1109 165 StyI C'CwwG_G Cuts at: 0 617 985 1125 1884 2429 2739 Size: 617 368 140 759 545 310 Fragments arranged by size: 759 617 545 368 310 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2308 2739 Size: 2308 431 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 720 752 1492 1524 2739 Size: 720 32 740 32 1215 Fragments arranged by size: 1215 740 720 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1788 2739 Size: 1788 951 XmnI GAAnn'nnTTC Cuts at: 0 286 863 1893 2739 Size: 286 577 1030 846 Fragments arranged by size: 1030 846 577 286 Enzymes that do cut: AarI AccI AcuI AlfI AlwNI ApaLI ApoI BanI BbsI BbvCI BcgI BciVI BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI DraI DraIII DrdI EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PshAI PstI PvuII SapI SexAI SfcI SmlI SspI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BclI BglI BlpI BmgBI BmtI BplI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstEII Bsu36I BtgZI ClaI CspCI EaeI EagI EciI EcoICRI EcoO109I FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XhoI XmaI ZraI