################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 196 GTCTAC GT^[AC][GT]AC AccII 59 CGCG CG^CG AfaI 346 GTAC GT^AC AflIII 200 ACATGT A^C[GA][CT]GT BclI 287 TGATCA T^GATCA BcnI 450 CCCGG CC^[GC]GG BglI 368 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 243 CTGGAG CTGGAG(16/14) BsaAI 363 TACGTG [CT]AC^GT[GA] BsaBI 111 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 241 CCCTGG C^C[ACGT][ACGT]GG BseRI 147 CTCCTC GAGGAG(10/8) BspLU11I 200 ACATGT A^CATGT BspMI 209 ACCTGC ACCTGC(4/8) BstUI 59 CGCG CG^CG Cac8I 43 GCCTGC GC[ACGT]^[ACGT]GC FauI 439 CCCGC CCCGC(4/6) FbaI 287 TGATCA T^GATCA HaeII 14 AGCGCC [GA]GCGC^[CT] HapII 451 CCGG C^CGG HpaII 451 CCGG C^CGG MaeII 364 ACGT A^CGT MamI 111 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 254 TCTTC GAAGA(8/7) MspI 451 CCGG C^CGG MvnI 59 CGCG CG^CG MwoI 368 GCCTCTTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 450 CCCGG CC^[GC]GG NlaIII 201 CATG CATG^ NlaIV 24 GGCTCC GG[ACGT]^[ACGT]CC NspI 200 ACATGT [GA]CATG^[CT] RsaI 346 GTAC GT^AC SfaNI 382 GATGC GCATC(5/9) TaqI 66 TCGA T^CGA TthHB8I 66 TCGA T^CGA XcmI 226 CCATTCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 111 GATCC GGATC(4/5) 171 GATCC BsgI 45 CTGCAC GTGCAG(16/14) 211 CTGCAC BsiYI 264 CCTCATAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 439 CCCGCAGATGG BslI 264 CCTCATAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 439 CCCGCAGATGG BsrI 236 ACTGG ACTGG(1/-1) 348 ACTGG CfoI 15 GCGC GCG^C 60 GCGC DdeI 11 CTGAG C^T[ACGT]AG 89 CTGAG HhaI 15 GCGC GCG^C 60 GCGC HinP1I 15 GCGC G^CGC 60 GCGC HinfI 7 GATTC G^A[ACGT]TC 74 GAATC MaeIII 206 GTGAC ^GT[ACGT]AC 391 GTCAC TfiI 7 GATTC G^A[AT]TC 74 GAATC Tsp45I 206 GTGAC ^GT[GC]AC 391 GTCAC TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^ 432 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI BbvI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HaeIII HphI ItaI MboI MnlI MvaI NdeII Sau3AI Sau96I ScrFI TseI